| Definition | Rhodopseudomonas palustris TIE-1 chromosome, complete genome. |
|---|---|
| Accession | NC_011004 |
| Length | 5,744,041 |
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The map label for this gene is paaF [C]
Identifier: 192291480
GI number: 192291480
Start: 3348720
End: 3349547
Strand: Reverse
Name: paaF [C]
Synonym: Rpal_3104
Alternate gene names: 192291480
Gene position: 3349547-3348720 (Counterclockwise)
Preceding gene: 192291486
Following gene: 192291479
Centisome position: 58.31
GC content: 66.3
Gene sequence:
>828_bases ATGACCGTGTTGCCCGCTTTCGCAGCGACCCAGCCTTCACCGATTCTGCTGCGTGAGACCGTCGATGGCGGTGTTGCAGT GCTGACGTTGAACAGACCGGCCGCGCGCAACAGCCTGTCGCTGGAAATGATCGACGATCTGCACGCGGCGCTGGATCTGA TCGCGCGCGACGATGGCGTGCGTGTCGTGGTTATCGCCGCCAATGGCCCGGCGTTCTGCGCCGGCCATGACCTCAAAGAG CTGACCGCGCATCGCAATGACGCCGATCGCGGCCGTGCCAGCTTCACCCGGATCATGACCGCCTGCAGTGCGATGATGCA GGCGATCGTCAAGCTGCCCAAGCCGGTGATCGCGGCGGTGCAGGGCGTCGCCACCGCGGCGGGCTGTCAGCTGGTCGCGA GCTGCGACCTCGCGATCGCATCCGAGCAGGCGACCTTCGCGACGCCCGGCGTCGATATCGGACTGTTCTGCTCCACCCCG ATGGTGGCGCTGTCGCGCAACGTGCCGCGCAAGAACGCGATGCACATGCTACTGACCGGCGAGCCGGTCGGCGCCGAAGA GGCCCGCCAGATCGGCCTGATCAACCGCGTGGTGCCGCACGGCACCGAGCGCGCCGCGGCCGTCGCACTGGCCGAGCAGA TCGCCGGCAAGTCGGCCCACACCATCAAGATCGGCAAGGAGGCGTTCTACCGCCAGGCCGAGCTCAACCTCGCCGACGCC TATCACTACGCCTCGCAGGTGATGGCCGAGAACATGATGGCCGCCGATGCCGAAGAAGGCATTTGTGCCTTCCTGGAAAA GCGCCAGCCGGAATGGAAGGATCGTTGA
Upstream 100 bases:
>100_bases CATGTGTCATGTCGTTCCCGCCCCTGCTCTCGCTTCGTTCGGCTTGTTACAATAAGGTGACGCTCGTTCCCAGCGCTTCA TTTTGTTCCCGGACGTCGCC
Downstream 100 bases:
>100_bases TCCGCGGCGTCGACCATGCCCCGTCATTGCGAGCGAAGCGAAGCAATCCAGGGCCAAGCACCGGAAGCTGGATTGCTTCG TCGCGGAGCCTGTGCTCGGA
Product: enoyl-CoA hydratase
Products: NA
Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]
Number of amino acids: Translated: 275; Mature: 274
Protein sequence:
>275_residues MTVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGVRVVVIAANGPAFCAGHDLKE LTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAVQGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTP MVALSRNVPRKNAMHMLLTGEPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR
Sequences:
>Translated_275_residues MTVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGVRVVVIAANGPAFCAGHDLKE LTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAVQGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTP MVALSRNVPRKNAMHMLLTGEPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR >Mature_274_residues TVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGVRVVVIAANGPAFCAGHDLKEL TAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAVQGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTPM VALSRNVPRKNAMHMLLTGEPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADAY HYASQVMAENMMAADAEEGICAFLEKRQPEWKDR
Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]
Homologues:
Organism=Homo sapiens, GI31542718, Length=263, Percent_Identity=45.6273764258555, Blast_Score=239, Evalue=2e-63, Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=32.9411764705882, Blast_Score=134, Evalue=1e-31, Organism=Homo sapiens, GI4502327, Length=267, Percent_Identity=28.0898876404494, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI221136756, Length=261, Percent_Identity=26.8199233716475, Blast_Score=89, Evalue=5e-18, Organism=Homo sapiens, GI221136753, Length=261, Percent_Identity=26.8199233716475, Blast_Score=89, Evalue=5e-18, Organism=Homo sapiens, GI4757968, Length=268, Percent_Identity=26.865671641791, Blast_Score=88, Evalue=7e-18, Organism=Homo sapiens, GI49355787, Length=268, Percent_Identity=26.865671641791, Blast_Score=88, Evalue=7e-18, Organism=Homo sapiens, GI221307494, Length=261, Percent_Identity=26.8199233716475, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=24.5136186770428, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=24.5136186770428, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=24.5136186770428, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI25453481, Length=255, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI51538573, Length=255, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI4757966, Length=185, Percent_Identity=30.2702702702703, Blast_Score=82, Evalue=5e-16, Organism=Homo sapiens, GI51538581, Length=185, Percent_Identity=30.2702702702703, Blast_Score=82, Evalue=5e-16, Organism=Homo sapiens, GI70995211, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=3e-15, Organism=Homo sapiens, GI116235487, Length=181, Percent_Identity=31.4917127071823, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=5e-13, Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI20127408, Length=186, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=2e-12, Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=35.3174603174603, Blast_Score=128, Evalue=5e-31, Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=4e-25, Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=35.1145038167939, Blast_Score=97, Evalue=2e-21, Organism=Escherichia coli, GI87082183, Length=253, Percent_Identity=28.0632411067194, Blast_Score=94, Evalue=7e-21, Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=27.2373540856031, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1790281, Length=187, Percent_Identity=24.5989304812834, Blast_Score=68, Evalue=7e-13, Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=27.8606965174129, Blast_Score=65, Evalue=5e-12, Organism=Caenorhabditis elegans, GI17540306, Length=271, Percent_Identity=44.280442804428, Blast_Score=214, Evalue=3e-56, Organism=Caenorhabditis elegans, GI17554946, Length=272, Percent_Identity=33.0882352941176, Blast_Score=143, Evalue=9e-35, Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=32.9365079365079, Blast_Score=125, Evalue=2e-29, Organism=Caenorhabditis elegans, GI17560910, Length=264, Percent_Identity=29.5454545454545, Blast_Score=100, Evalue=7e-22, Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=27.027027027027, Blast_Score=96, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=25.5725190839695, Blast_Score=94, Evalue=7e-20, Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=27.7056277056277, Blast_Score=88, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=31.6037735849057, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=28.0632411067194, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI25144160, Length=182, Percent_Identity=28.021978021978, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI25144157, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17558304, Length=222, Percent_Identity=24.3243243243243, Blast_Score=65, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6320241, Length=202, Percent_Identity=27.2277227722772, Blast_Score=81, Evalue=2e-16, Organism=Drosophila melanogaster, GI19922422, Length=270, Percent_Identity=39.2592592592593, Blast_Score=188, Evalue=3e-48, Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=31.8725099601594, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=31.8725099601594, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI24653139, Length=270, Percent_Identity=31.4814814814815, Blast_Score=118, Evalue=4e-27, Organism=Drosophila melanogaster, GI21357171, Length=273, Percent_Identity=27.4725274725275, Blast_Score=99, Evalue=4e-21, Organism=Drosophila melanogaster, GI24650670, Length=281, Percent_Identity=29.1814946619217, Blast_Score=97, Evalue=9e-21, Organism=Drosophila melanogaster, GI19920382, Length=241, Percent_Identity=27.8008298755187, Blast_Score=87, Evalue=9e-18, Organism=Drosophila melanogaster, GI24654903, Length=179, Percent_Identity=30.7262569832402, Blast_Score=83, Evalue=2e-16, Organism=Drosophila melanogaster, GI24583165, Length=181, Percent_Identity=29.2817679558011, Blast_Score=79, Evalue=4e-15, Organism=Drosophila melanogaster, GI19921018, Length=181, Percent_Identity=24.8618784530387, Blast_Score=66, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014748 - InterPro: IPR001753 - InterPro: IPR018376 [H]
Pfam domain/function: PF00378 ECH [H]
EC number: =4.2.1.116 [H]
Molecular weight: Translated: 29195; Mature: 29064
Theoretical pI: Translated: 6.39; Mature: 6.39
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.2 %Cys (Translated Protein) 4.0 %Met (Translated Protein) 6.2 %Cys+Met (Translated Protein) 2.2 %Cys (Mature Protein) 3.6 %Met (Mature Protein) 5.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGV CCCCCCHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC RVVVIAANGPAFCAGHDLKELTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAV EEEEEECCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH QGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTPMVALSRNVPRKNAMHMLLTG HHHHHHHHHHHEECCCEEEECCCCEEECCCCCEEEECCCCHHHHHCCCCCCCCEEEEEEC EPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCHHHH YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC >Mature Secondary Structure TVLPAFAATQPSPILLRETVDGGVAVLTLNRPAARNSLSLEMIDDLHAALDLIARDDGV CCCCCHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC RVVVIAANGPAFCAGHDLKELTAHRNDADRGRASFTRIMTACSAMMQAIVKLPKPVIAAV EEEEEECCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH QGVATAAGCQLVASCDLAIASEQATFATPGVDIGLFCSTPMVALSRNVPRKNAMHMLLTG HHHHHHHHHHHEECCCEEEECCCCEEECCCCCEEEECCCCHHHHHCCCCCCCCEEEEEEC EPVGAEEARQIGLINRVVPHGTERAAAVALAEQIAGKSAHTIKIGKEAFYRQAELNLADA CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCHHHH YHYASQVMAENMMAADAEEGICAFLEKRQPEWKDR HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA