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The map label for this gene is mdeA [H]

Identifier: 192291478

GI number: 192291478

Start: 3346611

End: 3347891

Strand: Reverse

Name: mdeA [H]

Synonym: Rpal_3102

Alternate gene names: 192291478

Gene position: 3347891-3346611 (Counterclockwise)

Preceding gene: 192291479

Following gene: 192291477

Centisome position: 58.28

GC content: 65.57

Gene sequence:

>1281_bases
ATGACCGAACGCAACCCAGGATTCGCGACGCTCGCGGTCCATGCCGGCGCCCAGCCCGATCCCACCACCGGTGCGCGCGC
GACGCCGATCTATCAGACCACATCGTTCGTCTTCAACGACGCCGACCATGCCGCCTCGCTGTTCGGCCTGCAGGCGTTCG
GCAACATCTACACCCGCATCACCAACCCGACGACGTCGGTGCTCGAAGAGCGCGTCGCCGCGCTCGAAGGCGGCACCGCC
GCGCTCGCCACCGCTTCGGGCCACGCCGCCCAGCTCGTCGCGCTGCAGCAACTGATGCAGCCCGGCGACGAGTTTATCGC
CGCGCGCAAACTCTACGGCGGCTCGATCAATCAGTTCACCCACGCCTTCAAGAGCTTCGGCTGGAACGTGGTGTGGGCCG
ACACCGACGACCTCGCCAGCTTCCAGCGGGCGGTGTCGCCGAAGACCAAGGCGATCTTCATCGAGTCGATCGCCAATCCG
GGCGGCAGCATCACCGACATCGAGGCGGTGGCGGAAGTCGCGCGCAATGCGGGCGTGCCGCTGATCGTCGACAACACGTT
GGCGACGCCCTACCTGATCCGCCCGATCGACCATGGCGCGGACATCGTGGTGCATTCGCTCACCAAGTTCCTGGGCGGCC
ACGGCAACTCGCTCGGTGGTATCATCGTCGATGCCGGCACCTTCGACTGGTCGAAGGACGGCAAATATCCGATGCTGAGC
GAGCCGCGCCCCGAATATCACGGCCTGAAGATCCAGGAGACGTTCGGCAACTTCTCGTTCGCGATTGCCTGCCGCGTGCT
CGGCCTGCGCGATCTCGGGCCCGCGCTGTCGCCGTTCAACGCCTTTCTGCTGCTGACCGGCATCGAGACGCTGCCGCTGC
GTATGCAGAAGCACTGCGAGAACGCCAAGGCGATTGCTGAATTCCTCTCCACCCACAAGGCGGTGGACGAGGTCAACTAC
TCCGGCCTCGCCTCCAGCAAATACGCCGCCCTCGCCCGCAAATATGCGCCGAAGGGCGCCGGCGCGGTGTTCACCTTCAG
CCTCAAGGGCGGCTATCAGGCGGGCATCGATCTGGTCGCCAATCTGAAGCTGTTCTCGCATTTGGCCAATGTCGGCGACA
CTCGCTCGCTGATCATCCACCCGGCCTCGACCACCCACAGCCAGCTTGACGACGCCCAGAAGACCGCCGCCGGCGCCGCG
CCCAACATGGTGCGGGTGTCGATCGGCATCGAGGACAAGGACGACCTGATCGCCGACCTCGATCAGGCACTCGGCGGCTG
A

Upstream 100 bases:

>100_bases
GTCTGAACGCTGCAGCACGCCTGCCCCGCCTGCGCTTGACCTGGTCCGGGGTTCCGATCAGATAGCCTAAACCATCAGCC
ACAGTACGGAGGGATCGATC

Downstream 100 bases:

>100_bases
CGCCGCGAGGCCGCTAGGGGATCGGCGATGACCGGCCATTTCGAAACGCGGCGGCTCGAGCAGCTCAGCAACACCATCTT
CGGTGTGGCGATGACGCTGC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTERNPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRITNPTTSVLEERVAALEGGTA
ALATASGHAAQLVALQQLMQPGDEFIAARKLYGGSINQFTHAFKSFGWNVVWADTDDLASFQRAVSPKTKAIFIESIANP
GGSITDIEAVAEVARNAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKDGKYPMLS
EPRPEYHGLKIQETFGNFSFAIACRVLGLRDLGPALSPFNAFLLLTGIETLPLRMQKHCENAKAIAEFLSTHKAVDEVNY
SGLASSKYAALARKYAPKGAGAVFTFSLKGGYQAGIDLVANLKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAA
PNMVRVSIGIEDKDDLIADLDQALGG

Sequences:

>Translated_426_residues
MTERNPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRITNPTTSVLEERVAALEGGTA
ALATASGHAAQLVALQQLMQPGDEFIAARKLYGGSINQFTHAFKSFGWNVVWADTDDLASFQRAVSPKTKAIFIESIANP
GGSITDIEAVAEVARNAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKDGKYPMLS
EPRPEYHGLKIQETFGNFSFAIACRVLGLRDLGPALSPFNAFLLLTGIETLPLRMQKHCENAKAIAEFLSTHKAVDEVNY
SGLASSKYAALARKYAPKGAGAVFTFSLKGGYQAGIDLVANLKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAA
PNMVRVSIGIEDKDDLIADLDQALGG
>Mature_425_residues
TERNPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRITNPTTSVLEERVAALEGGTAA
LATASGHAAQLVALQQLMQPGDEFIAARKLYGGSINQFTHAFKSFGWNVVWADTDDLASFQRAVSPKTKAIFIESIANPG
GSITDIEAVAEVARNAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKDGKYPMLSE
PRPEYHGLKIQETFGNFSFAIACRVLGLRDLGPALSPFNAFLLLTGIETLPLRMQKHCENAKAIAEFLSTHKAVDEVNYS
GLASSKYAALARKYAPKGAGAVFTFSLKGGYQAGIDLVANLKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAAP
NMVRVSIGIEDKDDLIADLDQALGG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=425, Percent_Identity=32.2352941176471, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI299473759, Length=425, Percent_Identity=28.9411764705882, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI25453487, Length=424, Percent_Identity=28.7735849056604, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790375, Length=413, Percent_Identity=30.2663438256659, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1789383, Length=425, Percent_Identity=25.6470588235294, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=31.1004784688995, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=423, Percent_Identity=33.3333333333333, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=31.9148936170213, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=45.9584295612009, Blast_Score=337, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=32.1513002364066, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=441, Percent_Identity=26.530612244898, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=26.5734265734266, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321170, Length=362, Percent_Identity=25.6906077348066, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=30.7328605200946, Blast_Score=184, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45137; Mature: 45006

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERNPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRI
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEHHHEECCCHHHHHHHHHHHHHHHHEEE
TNPTTSVLEERVAALEGGTAALATASGHAAQLVALQQLMQPGDEFIAARKLYGGSINQFT
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHH
HAFKSFGWNVVWADTDDLASFQRAVSPKTKAIFIESIANPGGSITDIEAVAEVARNAGVP
HHHHHCCCEEEEECCHHHHHHHHHCCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
LIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKDGKYPMLS
EEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
EPRPEYHGLKIQETFGNFSFAIACRVLGLRDLGPALSPFNAFLLLTGIETLPLRMQKHCE
CCCCCCCCEEEEEHHCCCHHEEHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHC
NAKAIAEFLSTHKAVDEVNYSGLASSKYAALARKYAPKGAGAVFTFSLKGGYQAGIDLVA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
NLKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAAPNMVRVSIGIEDKDDLIADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
DQALGG
HHHHCC
>Mature Secondary Structure 
TERNPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRI
CCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEHHHEECCCHHHHHHHHHHHHHHHHEEE
TNPTTSVLEERVAALEGGTAALATASGHAAQLVALQQLMQPGDEFIAARKLYGGSINQFT
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHH
HAFKSFGWNVVWADTDDLASFQRAVSPKTKAIFIESIANPGGSITDIEAVAEVARNAGVP
HHHHHCCCEEEEECCHHHHHHHHHCCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
LIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKDGKYPMLS
EEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
EPRPEYHGLKIQETFGNFSFAIACRVLGLRDLGPALSPFNAFLLLTGIETLPLRMQKHCE
CCCCCCCCEEEEEHHCCCHHEEHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHC
NAKAIAEFLSTHKAVDEVNYSGLASSKYAALARKYAPKGAGAVFTFSLKGGYQAGIDLVA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
NLKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAAPNMVRVSIGIEDKDDLIADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
DQALGG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]