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The map label for this gene is rimO [H]

Identifier: 192291328

GI number: 192291328

Start: 3222952

End: 3224277

Strand: Reverse

Name: rimO [H]

Synonym: Rpal_2950

Alternate gene names: 192291328

Gene position: 3224277-3222952 (Counterclockwise)

Preceding gene: 192291329

Following gene: 192291324

Centisome position: 56.13

GC content: 66.14

Gene sequence:

>1326_bases
ATGCAGCAGGCCACCGCGCCCAAGATCAGTTTTGTTTCGCTGGGATGCCCCAAGGCCCTGGTCGATTCCGAGCGCATCAT
CACCCGGTTGCGCGCCGAGGGCTACGAGCTGGCGCGCAAGCACGACGGCGCGGACCTCGTCATCGTCAACACCTGCGGCT
TCCTGGACAGTGCCAAGCAGGAATCGCTGGCGGCGATCGGCGAAGCGATGGCGACCAACGGCAAGGTGATCGTCACCGGC
TGCATGGGCGCCGAGCCGGAGCAGATCGAAGCCGCCTATCCCGGCGTGCTGTCGATCACCGGCCCGCAGCAATATGAGAG
CGTGCTCGACGCGGTGCATCGCGCCCGGCCGGCGCTGCACAACCCGCATCTCGATCTGGTCCCCCCGCAGGGCGTGCGGC
TAACGCCGCGGCACTATGCGTATTTGAAGATCTCAGAGGGCTGCAACAATCGCTGCAGCTTCTGCATCATCCCGAAGCTG
CGCGGCGATCTGGTATCACGCCCGGCCGGCGACGTGCTGCGCGAGGCCGAGAAGCTGGTCGCCGCCGGCGTCAAGGAGCT
GCTGGTGATCTCGCAGGACACCTCGGCCTACGGCGTCGATGTGAAGTACGCAGAGAGCCCGTGGAAGGACCGTGCCGTCC
GCGCCAAATTCCTCGATCTGGCCAGCGAACTCGGGGAGCTCGGCGCCTGGGTGCGGCTGCATTACGTCTATCCGTATCCG
CATGTCGACGAGGTGATCGGCTTGATGGCGGACGGCAAGGTGCTGCCTTATCTCGACATCCCATTCCAGCACGCCAGCCC
CGACGTGCTGAAGCTGATGAAGCGCCCGGCCGCTCAGGATAAGACGCTCGACCGGATCAAGCGCTGGCGCGAGCAGTGCC
CGGATCTGGCGCTGCGCTCGACCTTCATTGTGGGCTTCCCCGGCGAGACCGAGCGCGATTTCGAATTCCTGCTCGAATGG
CTCGACGAAGCAGAAATCGATCGTCTCGGCGCGTTCAAATACGAGCCGGTCGCCGGCGCACCGTCCAACGCCCTGCCCGA
TCAGATCGCGGACGAGGTCAAGCAGGAGCGCTGGAACAGGCTGATGGCGCGGCAGCAGGCGATCTCCGCCCGCCGGCTGA
AGCGCAAGGTCGGCACCCGCCAGCAGGTGATCATCGACGAGATCGGCCCGACCGTTACCAAAGGCCGCTCCAAGGCCGAC
GCGCCGGAGATCGACGGCGCCGTCTATCTGTCGAGCCGCCGCCCGCTGCGCGTCGGCGAAATCGTCACCGCCAAGATCGA
CCGCGCCGACGCGTACGACCTGCACGGGACCGTGGCGGGGTTCTAA

Upstream 100 bases:

>100_bases
CCAAAGAGCATTCTTCTCGCCTGAAGGAGTTCCGAGCCGCCTTGTCAGCGCGGCCGATCCGGTTTAGCTCCTCTCACACT
CCAATCCAGGCAGTTCGACC

Downstream 100 bases:

>100_bases
ACTCGCCTCTAACGCACGGCTCCTCGACATCTAATCACGTCATCGCCCGGCTTGTCCGGGCGACCCAGTATTGCAGAGCG
CTGATGTCAAGTAACAGGCG

Product: 30S ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQQATAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADLVIVNTCGFLDSAKQESLAAIGEAMATNGKVIVTG
CMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRARPALHNPHLDLVPPQGVRLTPRHYAYLKISEGCNNRCSFCIIPKL
RGDLVSRPAGDVLREAEKLVAAGVKELLVISQDTSAYGVDVKYAESPWKDRAVRAKFLDLASELGELGAWVRLHYVYPYP
HVDEVIGLMADGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLDRIKRWREQCPDLALRSTFIVGFPGETERDFEFLLEW
LDEAEIDRLGAFKYEPVAGAPSNALPDQIADEVKQERWNRLMARQQAISARRLKRKVGTRQQVIIDEIGPTVTKGRSKAD
APEIDGAVYLSSRRPLRVGEIVTAKIDRADAYDLHGTVAGF

Sequences:

>Translated_441_residues
MQQATAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADLVIVNTCGFLDSAKQESLAAIGEAMATNGKVIVTG
CMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRARPALHNPHLDLVPPQGVRLTPRHYAYLKISEGCNNRCSFCIIPKL
RGDLVSRPAGDVLREAEKLVAAGVKELLVISQDTSAYGVDVKYAESPWKDRAVRAKFLDLASELGELGAWVRLHYVYPYP
HVDEVIGLMADGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLDRIKRWREQCPDLALRSTFIVGFPGETERDFEFLLEW
LDEAEIDRLGAFKYEPVAGAPSNALPDQIADEVKQERWNRLMARQQAISARRLKRKVGTRQQVIIDEIGPTVTKGRSKAD
APEIDGAVYLSSRRPLRVGEIVTAKIDRADAYDLHGTVAGF
>Mature_441_residues
MQQATAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADLVIVNTCGFLDSAKQESLAAIGEAMATNGKVIVTG
CMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRARPALHNPHLDLVPPQGVRLTPRHYAYLKISEGCNNRCSFCIIPKL
RGDLVSRPAGDVLREAEKLVAAGVKELLVISQDTSAYGVDVKYAESPWKDRAVRAKFLDLASELGELGAWVRLHYVYPYP
HVDEVIGLMADGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLDRIKRWREQCPDLALRSTFIVGFPGETERDFEFLLEW
LDEAEIDRLGAFKYEPVAGAPSNALPDQIADEVKQERWNRLMARQQAISARRLKRKVGTRQQVIIDEIGPTVTKGRSKAD
APEIDGAVYLSSRRPLRVGEIVTAKIDRADAYDLHGTVAGF

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=399, Percent_Identity=29.0726817042606, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=24.8953974895398, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=24.8953974895398, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787057, Length=432, Percent_Identity=65.2777777777778, Blast_Score=584, Evalue=1e-168,
Organism=Escherichia coli, GI1786882, Length=401, Percent_Identity=27.930174563591, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=479, Percent_Identity=24.2171189979123, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=28.90625, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=24.8953974895398, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922432, Length=371, Percent_Identity=27.4932614555256, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 48770; Mature: 48770

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQATAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADLVIVNTCGFLDSAKQ
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
ESLAAIGEAMATNGKVIVTGCMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRARPALH
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
NPHLDLVPPQGVRLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPAGDVLREAEKLV
CCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCHHHHHHCCCHHHHHHHHHHHH
AAGVKELLVISQDTSAYGVDVKYAESPWKDRAVRAKFLDLASELGELGAWVRLHYVYPYP
HHCHHHHEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
HVDEVIGLMADGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLDRIKRWREQCPDLALRS
CHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHECC
TFIVGFPGETERDFEFLLEWLDEAEIDRLGAFKYEPVAGAPSNALPDQIADEVKQERWNR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMARQQAISARRLKRKVGTRQQVIIDEIGPTVTKGRSKADAPEIDGAVYLSSRRPLRVGE
HHHHHHHHHHHHHHHHHCCHHEEHHHHCCCCHHCCCCCCCCCCCCCEEEECCCCCEECCC
IVTAKIDRADAYDLHGTVAGF
EEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQATAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADLVIVNTCGFLDSAKQ
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
ESLAAIGEAMATNGKVIVTGCMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRARPALH
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
NPHLDLVPPQGVRLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPAGDVLREAEKLV
CCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCHHHHHHCCCHHHHHHHHHHHH
AAGVKELLVISQDTSAYGVDVKYAESPWKDRAVRAKFLDLASELGELGAWVRLHYVYPYP
HHCHHHHEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
HVDEVIGLMADGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLDRIKRWREQCPDLALRS
CHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHECC
TFIVGFPGETERDFEFLLEWLDEAEIDRLGAFKYEPVAGAPSNALPDQIADEVKQERWNR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMARQQAISARRLKRKVGTRQQVIIDEIGPTVTKGRSKADAPEIDGAVYLSSRRPLRVGE
HHHHHHHHHHHHHHHHHCCHHEEHHHHCCCCHHCCCCCCCCCCCCCEEEECCCCCEECCC
IVTAKIDRADAYDLHGTVAGF
EEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA