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The map label for this gene is rutF [H]

Identifier: 192291258

GI number: 192291258

Start: 3142127

End: 3142696

Strand: Reverse

Name: rutF [H]

Synonym: Rpal_2880

Alternate gene names: 192291258

Gene position: 3142696-3142127 (Counterclockwise)

Preceding gene: 192291259

Following gene: 192291257

Centisome position: 54.71

GC content: 64.74

Gene sequence:

>570_bases
ATGACGCTTGCCATCGAGGACCGTCTTCGTTCCGCCTCGCCAGCCGAGTTCGCCCATGCGATGCGGCAGCTCGCGAGCGG
CGTCAGCGTCATCACCGCCGGCAAAGGCAGCACCCGAGTCGGCATGACCGCCACGGCGGTGACCTCGCTGTCGGCGACGC
CGCCGACGTTGATCGTCTGTCTCAACCAGAAGTCTTCCGCCGGCCCGCTGATCGCTTGCAGTCACGCCTTCGCGGTCAAC
ATCCTCGCTGCCGACCAGATCGAAATCGGCGAACGCTTCGCCGGCAAGGGCGGCCTGAAGGGCGCCGCGCGGTTCGCCGG
TGGTACATGGAATACCGGCATCTCGGGCGCTCCCGTTTTAGCAGGCGCACTCGCGAACATCGAATGCGAGCTCGATGAAG
TTATCGAGCGGCATACCCACGCGATCTTGGTTGGCCGCGTCGTTCACACCTCGACGCTGCCACGCAAAGCCTCACTCACC
TACTGGCAGGGCAGCTACGTCGCCATTGATCGCGACGAAGATCTCGTGCGCCTGGCCGAGGTCAGCGTACCAAGCGCCAA
GCACGGTTAG

Upstream 100 bases:

>100_bases
CGCCAGCGCGGCTCCGCCGAGCTCGCCGCACTCGAAGGTGAGATCCTAAGAGATCTGCTGAAGGGCGCGGAGGACACATT
CGAGCTGTGAGGCTGCTGCC

Downstream 100 bases:

>100_bases
CGTCGAATCCGAAAGCTGACTTACGAACCTGGCAAACAGTCCTTTCCCCAATGAGACGCCGCGCAGCATAGTTCGCACGA
CTGGCTTCGATCTTTGGAAT

Product: flavin reductase domain-containing protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MTLAIEDRLRSASPAEFAHAMRQLASGVSVITAGKGSTRVGMTATAVTSLSATPPTLIVCLNQKSSAGPLIACSHAFAVN
ILAADQIEIGERFAGKGGLKGAARFAGGTWNTGISGAPVLAGALANIECELDEVIERHTHAILVGRVVHTSTLPRKASLT
YWQGSYVAIDRDEDLVRLAEVSVPSAKHG

Sequences:

>Translated_189_residues
MTLAIEDRLRSASPAEFAHAMRQLASGVSVITAGKGSTRVGMTATAVTSLSATPPTLIVCLNQKSSAGPLIACSHAFAVN
ILAADQIEIGERFAGKGGLKGAARFAGGTWNTGISGAPVLAGALANIECELDEVIERHTHAILVGRVVHTSTLPRKASLT
YWQGSYVAIDRDEDLVRLAEVSVPSAKHG
>Mature_188_residues
TLAIEDRLRSASPAEFAHAMRQLASGVSVITAGKGSTRVGMTATAVTSLSATPPTLIVCLNQKSSAGPLIACSHAFAVNI
LAADQIEIGERFAGKGGLKGAARFAGGTWNTGISGAPVLAGALANIECELDEVIERHTHAILVGRVVHTSTLPRKASLTY
WQGSYVAIDRDEDLVRLAEVSVPSAKHG

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=157, Percent_Identity=36.3057324840764, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19615; Mature: 19483

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAIEDRLRSASPAEFAHAMRQLASGVSVITAGKGSTRVGMTATAVTSLSATPPTLIVC
CEEEECHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCEECEEHHHEEECCCCCCEEEEE
LNQKSSAGPLIACSHAFAVNILAADQIEIGERFAGKGGLKGAARFAGGTWNTGISGAPVL
ECCCCCCCCEEEECCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHH
AGALANIECELDEVIERHTHAILVGRVVHTSTLPRKASLTYWQGSYVAIDRDEDLVRLAE
HHHHHCCEECHHHHHHHHHHEEEEEHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEHHH
VSVPSAKHG
CCCCCCCCC
>Mature Secondary Structure 
TLAIEDRLRSASPAEFAHAMRQLASGVSVITAGKGSTRVGMTATAVTSLSATPPTLIVC
EEEECHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCEECEEHHHEEECCCCCCEEEEE
LNQKSSAGPLIACSHAFAVNILAADQIEIGERFAGKGGLKGAARFAGGTWNTGISGAPVL
ECCCCCCCCEEEECCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHH
AGALANIECELDEVIERHTHAILVGRVVHTSTLPRKASLTYWQGSYVAIDRDEDLVRLAE
HHHHHCCEECHHHHHHHHHHEEEEEHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEHHH
VSVPSAKHG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA