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The map label for this gene is nifH2 [H]

Identifier: 192290980

GI number: 192290980

Start: 2807997

End: 2808866

Strand: Reverse

Name: nifH2 [H]

Synonym: Rpal_2597

Alternate gene names: 192290980

Gene position: 2808866-2807997 (Counterclockwise)

Preceding gene: 192290981

Following gene: 192290979

Centisome position: 48.9

GC content: 65.29

Gene sequence:

>870_bases
ATGGCCAACAAATTGAAGCAGATCGCCATCTATGGAAAAGGCGGGATCGGCAAGTCCACCACCACCTCCAACATCAGTGC
GGCCCTTGCGGAGGCCGGCTACAAGGTGATGCAGTTCGGCGCCGACCCGAAGGCTGACTCGACCAACACGCTCCGCGGCG
GCGCCTACATCCCGTCGGTGCTCGACCTGCTGGCGGAACGCCGCCGTGTCGATGCCTACGAGGCGATCTTTCAAGGCTTC
GGCGGAATCTATTGCGTAGAGGCCGGCGGCCCGCAGCCCGGCGTCGGTTGCGCCGGCCGTGGCATCATCACCGCGGTCGA
ACTTCTGAAGCAGCAGCGGGTGTTCGAAGAACTCGACCTCGATTACGTGATCTACGACGTGCTGGGGGACGTGGTGTGCG
GCGGCTTCGCGGTGCCGGTGCGCGAGGGAATCGCCGAACATGTGTTCACCGTGTCGTCGTCCGACTTCATGGCGGTCTAT
GCCGCCAACAACCTGTTTCGCGGCATCGAGCGGTTCTCCAACTCGGGCGGCGCCCTGCTCGGCGGCATCATCGCCAATTC
GATCAATACCGACTTCCAGCGCGACGTGATCGACGATTTCGCCGCGCGCACCGTGACACCGATCGTCCAATACGTGCCGC
GCTCGCTGACCGTCACCCAGGCCGAACTCAGCGGACGCACAACGATCGAAGCTGCACCGCAATCCGCGCAGGCCGACATC
TATCGCCAGCTCGCGCGCCGCATCGCCGAGCACACCGAGTCCAAGGTCCCCACGCCGCTCGATCCGCAGCAGCTCCGCGA
ATGGTCGAGCAGCTGGGCGGATCGGCTGGTCGCGGCGGAACAAGCCGAGCGCGACCGCGCGGTCGCCTGA

Upstream 100 bases:

>100_bases
CGCCGCGCTTTACGGCGACGATCTCCTTGCCGAGCCAACCTTTTCGCACGGCTGACGCCGTCCTCCACACTCATTGAACA
ATTCTGAATAGGAGCCGACC

Downstream 100 bases:

>100_bases
CGCTCCCCGACTCCACCCTGTAGGCACTCAAGAGGACCAAAGATGGCAACTCTCCTGTTCGACGATGGACGCGAGGCGAC
GGCCGCTGAAATCGAGGCAA

Product: nitrogenase iron protein

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MANKLKQIAIYGKGGIGKSTTTSNISAALAEAGYKVMQFGADPKADSTNTLRGGAYIPSVLDLLAERRRVDAYEAIFQGF
GGIYCVEAGGPQPGVGCAGRGIITAVELLKQQRVFEELDLDYVIYDVLGDVVCGGFAVPVREGIAEHVFTVSSSDFMAVY
AANNLFRGIERFSNSGGALLGGIIANSINTDFQRDVIDDFAARTVTPIVQYVPRSLTVTQAELSGRTTIEAAPQSAQADI
YRQLARRIAEHTESKVPTPLDPQQLREWSSSWADRLVAAEQAERDRAVA

Sequences:

>Translated_289_residues
MANKLKQIAIYGKGGIGKSTTTSNISAALAEAGYKVMQFGADPKADSTNTLRGGAYIPSVLDLLAERRRVDAYEAIFQGF
GGIYCVEAGGPQPGVGCAGRGIITAVELLKQQRVFEELDLDYVIYDVLGDVVCGGFAVPVREGIAEHVFTVSSSDFMAVY
AANNLFRGIERFSNSGGALLGGIIANSINTDFQRDVIDDFAARTVTPIVQYVPRSLTVTQAELSGRTTIEAAPQSAQADI
YRQLARRIAEHTESKVPTPLDPQQLREWSSSWADRLVAAEQAERDRAVA
>Mature_288_residues
ANKLKQIAIYGKGGIGKSTTTSNISAALAEAGYKVMQFGADPKADSTNTLRGGAYIPSVLDLLAERRRVDAYEAIFQGFG
GIYCVEAGGPQPGVGCAGRGIITAVELLKQQRVFEELDLDYVIYDVLGDVVCGGFAVPVREGIAEHVFTVSSSDFMAVYA
ANNLFRGIERFSNSGGALLGGIIANSINTDFQRDVIDDFAARTVTPIVQYVPRSLTVTQAELSGRTTIEAAPQSAQADIY
RQLARRIAEHTESKVPTPLDPQQLREWSSSWADRLVAAEQAERDRAVA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31083; Mature: 30952

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKLKQIAIYGKGGIGKSTTTSNISAALAEAGYKVMQFGADPKADSTNTLRGGAYIPSV
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHH
LDLLAERRRVDAYEAIFQGFGGIYCVEAGGPQPGVGCAGRGIITAVELLKQQRVFEELDL
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
DYVIYDVLGDVVCGGFAVPVREGIAEHVFTVSSSDFMAVYAANNLFRGIERFSNSGGALL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCEEEEHHHHHHHHHHHHHCCCCCHHH
GGIIANSINTDFQRDVIDDFAARTVTPIVQYVPRSLTVTQAELSGRTTIEAAPQSAQADI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEECCCCHHHHHH
YRQLARRIAEHTESKVPTPLDPQQLREWSSSWADRLVAAEQAERDRAVA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANKLKQIAIYGKGGIGKSTTTSNISAALAEAGYKVMQFGADPKADSTNTLRGGAYIPSV
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHH
LDLLAERRRVDAYEAIFQGFGGIYCVEAGGPQPGVGCAGRGIITAVELLKQQRVFEELDL
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
DYVIYDVLGDVVCGGFAVPVREGIAEHVFTVSSSDFMAVYAANNLFRGIERFSNSGGALL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCEEEEHHHHHHHHHHHHHCCCCCHHH
GGIIANSINTDFQRDVIDDFAARTVTPIVQYVPRSLTVTQAELSGRTTIEAAPQSAQADI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEECCCCHHHHHH
YRQLARRIAEHTESKVPTPLDPQQLREWSSSWADRLVAAEQAERDRAVA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA