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The map label for this gene is groEL [H]

Identifier: 192290841

GI number: 192290841

Start: 2653092

End: 2654729

Strand: Reverse

Name: groEL [H]

Synonym: Rpal_2457

Alternate gene names: 192290841

Gene position: 2654729-2653092 (Counterclockwise)

Preceding gene: 192290842

Following gene: 192290840

Centisome position: 46.22

GC content: 65.32

Gene sequence:

>1638_bases
ATGTCCGCTAAAGAAGTCAAATTCGGCGTCGACGCCCGCGACCGCATGCTGCGTGGCGTGGACATTCTCGCCAATGCCGT
GAAGGTCACGCTGGGTCCGAAGGGCCGCAACGTCGTCCTCGACAAGTCGTTCGGCGCGCCGCGCATCACCAAGGACGGCG
TCACCGTCGCCAAGGACATCGAGCTCGACGACAAGTTCGAGAACATGGGCGCGCAGATGGTGCGCGAAGTCGCCTCGAAG
TCGGCCGACGCCGCGGGTGACGGCACCACCACCGCGACCGTGCTGGCCCAGGCGATCGTCCGCGAAGGCGCCAAGGCGGT
TGCCGCCGGCATGAACCCGATGGATCTGAAGCGCGGTATCGATCTGGCGGTGGAAGCCGTCGTCGCCGACCTCGTCAAGA
ACTCCAAGAAGGTCACCTCGAACGACGAGATTGCCCAGGTCGGCACCATCTCGGCCAACGGTGACGCCGAGATCGGCAAG
TTCCTCGCCGACGCGATGAAGAAGGTCGGCAACGAGGGTGTCATCACCGTCGAGGAAGCCAAGTCGCTCGAGACCGAACT
CGACGTCGTCGAGGGCATGCAGTTCGACCGCGGCTACATCTCGCCCTACTTCGTCACCAACGCCGACAAGATGCGCGTCG
AATTCGACGACGCCTACATCCTGATCAATGAGAAGAAGCTCTCCAACCTCAACGAGCTGCTGCCGCTGCTCGAGGCGGTG
GTGCAGACCGGCAAGCCGCTGGTGATCGTTGCGGAAGACGTCGAAGGCGAGGCTCTCGCCACCCTCGTCGTCAACCGTCT
GCGCGGCGGCCTCAAGGTCGCGGCCGTCAAGGCGCCGGGCTTCGGTGATCGCCGCAAGGCCATGCTGCAGGACATCGCGA
TCCTGACCGGCGGCCAGGCGATCTCGGAAGACCTCGGCATCAAGATGGAGAACGTCACCCTGCAGATGCTGGGTCGCGCC
AAGAAGGTGATGATCGACAAGGAAAACACCACGATCGTCAACGGCGCCGGCAAGAAGGCCGACATCGAGGCCCGCGTCGC
ACAGATCAAGGCGCAGATCGAGGAAACCACCTCGGACTACGACCGCGAGAAGCTGCAGGAGCGTCTGGCCAAGCTCGCCG
GCGGCGTCGCGGTGATCCGCGTCGGCGGTGCGACCGAGGTCGAGGTGAAGGAGCGCAAGGATCGCGTTGATGACGCGATG
CACGCCACCCGCGCCGCGGTCGAAGAAGGCATCGTCCCGGGCGGCGGCGTCGCACTGCTGCGCGCCTCCGAGCAGCTCAA
GGGCCTCAAGACCAAGAACGACGACCAGAAGACCGGCGTCGAGATCGTGCGCCGCGCCCTCTCCGCTCCGGCCCGCCAGA
TCGCCATCAACGCCGGCGAAGATGGCTCGGTGATCGTCGGCAAGGTGCTCGAGAAGGAGCAGTACGCGTTCGGCTTCGAC
TCGCAGTCGGGCGAATACGGCGACCTGGTCAAGAAGGGCATCATCGACCCGACCAAGGTGGTGCGCACCGCGATCCAGAA
CGCCGCCTCGGTGGCCGCGCTGCTGATCACCACCGAAGCGATGATCGCCGAACTGCCGAAGAAGAACGCCGGCCCCGCAA
TGCCCCCGGGCGGCGGCATGGGCGGCATGGACTTCTAA

Upstream 100 bases:

>100_bases
ACGTTCATCGCCTCGCCCTTCGAGACGCCGCCCTTGCCCCTCAGGTTCGGTCGGCTCCTCGGGATGAGGACACAACGATC
CAACCCAAGGGAAACCTCTT

Downstream 100 bases:

>100_bases
GCGAAGTCACCATCGCCACAGACAACGAAGCCCGGCGGAAACGCCGGGCTTTTTTGTTGGCGCAAGCAGGGCCGCAGAGG
TTCTCGTTGGCCATTCCGTG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKDIELDDKFENMGAQMVREVASK
SADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKMENVTLQMLGRA
KKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAM
HATRAAVEEGIVPGGGVALLRASEQLKGLKTKNDDQKTGVEIVRRALSAPARQIAINAGEDGSVIVGKVLEKEQYAFGFD
SQSGEYGDLVKKGIIDPTKVVRTAIQNAASVAALLITTEAMIAELPKKNAGPAMPPGGGMGGMDF

Sequences:

>Translated_545_residues
MSAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKDIELDDKFENMGAQMVREVASK
SADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKMENVTLQMLGRA
KKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAM
HATRAAVEEGIVPGGGVALLRASEQLKGLKTKNDDQKTGVEIVRRALSAPARQIAINAGEDGSVIVGKVLEKEQYAFGFD
SQSGEYGDLVKKGIIDPTKVVRTAIQNAASVAALLITTEAMIAELPKKNAGPAMPPGGGMGGMDF
>Mature_544_residues
SAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKDIELDDKFENMGAQMVREVASKS
ADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGKF
LADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAVV
QTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKMENVTLQMLGRAK
KVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMH
ATRAAVEEGIVPGGGVALLRASEQLKGLKTKNDDQKTGVEIVRRALSAPARQIAINAGEDGSVIVGKVLEKEQYAFGFDS
QSGEYGDLVKKGIIDPTKVVRTAIQNAASVAALLITTEAMIAELPKKNAGPAMPPGGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=55.3672316384181, Blast_Score=590, Evalue=1e-168,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=55.3672316384181, Blast_Score=590, Evalue=1e-168,
Organism=Homo sapiens, GI5453607, Length=539, Percent_Identity=23.0055658627087, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=65.6603773584906, Blast_Score=693, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=54.4423440453686, Blast_Score=576, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI193210679, Length=240, Percent_Identity=50, Blast_Score=227, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=57.4144486692015, Blast_Score=605, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6322524, Length=192, Percent_Identity=25, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322350, Length=545, Percent_Identity=23.302752293578, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=58.3333333333333, Blast_Score=613, Evalue=1e-175,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=58.3333333333333, Blast_Score=613, Evalue=1e-175,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=54.0952380952381, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=54.0952380952381, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=54.0952380952381, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI17864606, Length=546, Percent_Identity=47.2527472527472, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24584129, Length=536, Percent_Identity=36.7537313432836, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI19921262, Length=536, Percent_Identity=36.7537313432836, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57779; Mature: 57648

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKDI
CCCCCEEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCEEEECC
ELDDKFENMGAQMVREVASKSADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCH
DLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCEEEEEECHHHCCHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKMENVTLQMLGRAKKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDY
HHHHHCCEEHHHHHHHHCCCHHEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RASEQLKGLKTKNDDQKTGVEIVRRALSAPARQIAINAGEDGSVIVGKVLEKEQYAFGFD
ECHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHHHEECCC
SQSGEYGDLVKKGIIDPTKVVRTAIQNAASVAALLITTEAMIAELPKKNAGPAMPPGGGM
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GGMDF
CCCCC
>Mature Secondary Structure 
SAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKDI
CCCCEEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCEEEECC
ELDDKFENMGAQMVREVASKSADAAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCH
DLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCEEEEEECHHHCCHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKMENVTLQMLGRAKKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDY
HHHHHCCEEHHHHHHHHCCCHHEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RASEQLKGLKTKNDDQKTGVEIVRRALSAPARQIAINAGEDGSVIVGKVLEKEQYAFGFD
ECHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHHHEECCC
SQSGEYGDLVKKGIIDPTKVVRTAIQNAASVAALLITTEAMIAELPKKNAGPAMPPGGGM
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GGMDF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8101485; 12597275 [H]