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The map label for this gene is cobJ [H]

Identifier: 192290762

GI number: 192290762

Start: 2562355

End: 2563116

Strand: Reverse

Name: cobJ [H]

Synonym: Rpal_2378

Alternate gene names: 192290762

Gene position: 2563116-2562355 (Counterclockwise)

Preceding gene: 192290763

Following gene: 192290760

Centisome position: 44.62

GC content: 67.32

Gene sequence:

>762_bases
ATGACTGGATCAGTGGTGGTTGCCGGCCTCGGGCCCGGCGCTCAGGAACTGATCACGCCGGAAGTCAGCGCAGCGCTGGC
GCTAGCGACTGACATCGTCGGCTACGCACCTTATGTCGCTCGCGTCGCCGAACGCGACGGCTTGCAGCGGCACGCTTCCG
ACAATCGCGAAGAGCTCGATCGCGCCGGCTTCGCGCTGCGGCTGGCGACCGAGGGCCGGCACGTCGTGATCGTCTCCTCT
GGCGATCCGGGCGTGTTCGCGATGGCTGCGGCGCTGTTCGAAGCGATCGAGGCCGGCGATCCGTCGTGGCGCGAGCTCGA
CATCCGCGTGCTCCCCGGCATCAGCGCGATGTTCGCCGCCGCGGCGCGGATCGGCGCGCCGCTCGGCCACGATTTCTGCG
CGATCAACCTGTCGGACAATCTCAAGCCGTGGGAGACCGTCGAGAAGCGGCTGCGCGCGGCGGCCGAAGCCGATTTTGTG
ATCGCGCTGTATAACCCGATCTCGAAGGCACGGCCCTGGCAGCTCGGCCGCGCGTTCGAGCTGCTACGCTCCATACATCC
GACGTCGGTGCCGGTGGTCTTTGCGACCGCTATCAGCGATCCGCGCGAACGTATCGACGTGGCTCCGCTCGGCGACGCGG
TGCCGCAACGCGCCGACATGCGAACGCTGGTGATGATCGGCTCGTCGCAGACCCGGATCATTCCGCGCAGCAGCGGCGCT
GATTTCGTTTACACTCCGCGGTTCTCTGGAGCGGTGTCGTGA

Upstream 100 bases:

>100_bases
GCGCGATGGCCGATCAGGTGGTGATGCCGCTCGCCGAGAAGACGACCGACGACGCGCCCTACTTCTCGATTGTGCTGGTC
CACGGACAGGGGCGACGGCC

Downstream 100 bases:

>100_bases
CCGAGCCAAGCCATCACGTCGGCGACCGTGGCGACACTGCGCCGATCCGGCACCGCAGGCCGCTTCACCATGATCACCGG
CAGCCCAAGTTCGCGCGCCG

Product: precorrin-3B C17-methyltransferase

Products: NA

Alternate protein names: Precorrin-3 methylase; Precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTGSVVVAGLGPGAQELITPEVSAALALATDIVGYAPYVARVAERDGLQRHASDNREELDRAGFALRLATEGRHVVIVSS
GDPGVFAMAAALFEAIEAGDPSWRELDIRVLPGISAMFAAAARIGAPLGHDFCAINLSDNLKPWETVEKRLRAAAEADFV
IALYNPISKARPWQLGRAFELLRSIHPTSVPVVFATAISDPRERIDVAPLGDAVPQRADMRTLVMIGSSQTRIIPRSSGA
DFVYTPRFSGAVS

Sequences:

>Translated_253_residues
MTGSVVVAGLGPGAQELITPEVSAALALATDIVGYAPYVARVAERDGLQRHASDNREELDRAGFALRLATEGRHVVIVSS
GDPGVFAMAAALFEAIEAGDPSWRELDIRVLPGISAMFAAAARIGAPLGHDFCAINLSDNLKPWETVEKRLRAAAEADFV
IALYNPISKARPWQLGRAFELLRSIHPTSVPVVFATAISDPRERIDVAPLGDAVPQRADMRTLVMIGSSQTRIIPRSSGA
DFVYTPRFSGAVS
>Mature_252_residues
TGSVVVAGLGPGAQELITPEVSAALALATDIVGYAPYVARVAERDGLQRHASDNREELDRAGFALRLATEGRHVVIVSSG
DPGVFAMAAALFEAIEAGDPSWRELDIRVLPGISAMFAAAARIGAPLGHDFCAINLSDNLKPWETVEKRLRAAAEADFVI
ALYNPISKARPWQLGRAFELLRSIHPTSVPVVFATAISDPRERIDVAPLGDAVPQRADMRTLVMIGSSQTRIIPRSSGAD
FVYTPRFSGAVS

Specific function: Methyltransferase that catalyzes the methylation of C-17 in precorrin-3B to form precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.131 [H]

Molecular weight: Translated: 26985; Mature: 26854

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSVVVAGLGPGAQELITPEVSAALALATDIVGYAPYVARVAERDGLQRHASDNREELD
CCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHH
RAGFALRLATEGRHVVIVSSGDPGVFAMAAALFEAIEAGDPSWRELDIRVLPGISAMFAA
HCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHH
AARIGAPLGHDFCAINLSDNLKPWETVEKRLRAAAEADFVIALYNPISKARPWQLGRAFE
HHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHH
LLRSIHPTSVPVVFATAISDPRERIDVAPLGDAVPQRADMRTLVMIGSSQTRIIPRSSGA
HHHHCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCC
DFVYTPRFSGAVS
CEEECCCCCCCCC
>Mature Secondary Structure 
TGSVVVAGLGPGAQELITPEVSAALALATDIVGYAPYVARVAERDGLQRHASDNREELD
CCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHH
RAGFALRLATEGRHVVIVSSGDPGVFAMAAALFEAIEAGDPSWRELDIRVLPGISAMFAA
HCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHH
AARIGAPLGHDFCAINLSDNLKPWETVEKRLRAAAEADFVIALYNPISKARPWQLGRAFE
HHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHH
LLRSIHPTSVPVVFATAISDPRERIDVAPLGDAVPQRADMRTLVMIGSSQTRIIPRSSGA
HHHHCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCC
DFVYTPRFSGAVS
CEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211521; 8226690 [H]