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The map label for this gene is vdh [H]

Identifier: 192290325

GI number: 192290325

Start: 2083555

End: 2084754

Strand: Reverse

Name: vdh [H]

Synonym: Rpal_1932

Alternate gene names: 192290325

Gene position: 2084754-2083555 (Counterclockwise)

Preceding gene: 192290326

Following gene: 192290324

Centisome position: 36.29

GC content: 62.75

Gene sequence:

>1200_bases
ATGAGCGAACGCGCACCCGTCACCGACTGGGTCAACGACTTCGACCACACCGATCCGCGCTGGACCGAAAATCCCTATCC
GATCTGGGACGAGCTGCGCTCGGCCAGTCCGCTGGTCCACACCGACCGGTTTCTCGGCTGCTACATGCCGACGACCTTCG
CGGCGGTGAAGGAAATCTCCTACGACACCGATCATTTCTCGTCGCGGCGGGTGATCGTCCGCAACGTCCGCTCGGAATCG
CCGCCGCCGGCGCCCCCGATCACGTCGGATCCGCCGGAGCACAAGCCTGCGAAGCGGCTGCTATTGCCACCGTTCACGCC
CGATGCGGTCGCCAAGCTCGAGCCACGGGTGCGCGCGATCTGCAACGAACTGATCGACGCCTTCATCGAGGACGAAGGCT
GCGACGCCGCCACCGCCTATACCAAGCACATTCCGGTCAAGACCATCTGCCACATGCTCGGCATTCCGGAAGACGACAGC
GACATCTTCATCCGTTGGATCCACGAGATCCTTGAACTCGGCATCAACGACGATGCCATCCTGATGAAGGCGGTGTTCGA
GATGAGCACCTATTTCCAGGGACATATCGCGCATCGCAAGCAGAAACCGACCGACGACCTGATTAGCACACTGATGAACG
CCCGGGACGATAAAGGGCAACCGCTGTCGGACGCCCATGTGCTCGGCTCGCTGCGGCTGCTGCTGATCGCAGGCATCGAC
ACCACCTGGAGCGCGATCGGCGCGGCGCTGTGGCACCTCGCCACGCATCCGGCCGAACGCGAACGCCTGCTCGCCGAACC
GGAGCTGATGCCGACTGCGATCGAGGAATTCCTGCGCGCCTATTCGCCGGTGACGATGGCCCGCGAGGTGATGAAGGAGA
CATCGATCGCCGGCTGTCCTGTGAAGCCGGGCAACATGGTGCTGCTGTCGTTCCCCACCGCTAATCGTGATCCATCGGTG
TTTCCCGAGGCCGACAAGGTGATGATCGACCGCAAGGAGAATCCGCACGTTGCGTTCGGGCTCGGCATCCATCGCTGCGT
CGGTTCCAATCTGGCGCGGATGGAGATGACCGTTGCGATCGAGGAGTGGCTGAAGCGGATTGCTTCGTTTAGGCTCGACC
CGTCACAAAAAGTGCGCTGGTCCGAGGGCACAGTCCGTGGACCGCGCAGCCTGCCGTTGTTGTTCGGCAGGCCGAGCTGA

Upstream 100 bases:

>100_bases
CTGAGAATGCGATCGTCATCGACGAAGCTTGATCACTCCGCCTTCTCCCGTTCGAGCGGCGCCAAGACCGCACCGATACT
CAGCAGAGGAAACGCCAGAC

Downstream 100 bases:

>100_bases
GCCCTCGCCCGAAAGGTCACCGATGTCCGACGATGCCGAACTGCCGCCGGAGTCCAAACTCACCCGCAGCAAGCAGCGCT
GGGCGGCAGAAGGCAAGTTT

Product: cytochrome P450

Products: NA

Alternate protein names: Cytochrome P450 [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSERAPVTDWVNDFDHTDPRWTENPYPIWDELRSASPLVHTDRFLGCYMPTTFAAVKEISYDTDHFSSRRVIVRNVRSES
PPPAPPITSDPPEHKPAKRLLLPPFTPDAVAKLEPRVRAICNELIDAFIEDEGCDAATAYTKHIPVKTICHMLGIPEDDS
DIFIRWIHEILELGINDDAILMKAVFEMSTYFQGHIAHRKQKPTDDLISTLMNARDDKGQPLSDAHVLGSLRLLLIAGID
TTWSAIGAALWHLATHPAERERLLAEPELMPTAIEEFLRAYSPVTMAREVMKETSIAGCPVKPGNMVLLSFPTANRDPSV
FPEADKVMIDRKENPHVAFGLGIHRCVGSNLARMEMTVAIEEWLKRIASFRLDPSQKVRWSEGTVRGPRSLPLLFGRPS

Sequences:

>Translated_399_residues
MSERAPVTDWVNDFDHTDPRWTENPYPIWDELRSASPLVHTDRFLGCYMPTTFAAVKEISYDTDHFSSRRVIVRNVRSES
PPPAPPITSDPPEHKPAKRLLLPPFTPDAVAKLEPRVRAICNELIDAFIEDEGCDAATAYTKHIPVKTICHMLGIPEDDS
DIFIRWIHEILELGINDDAILMKAVFEMSTYFQGHIAHRKQKPTDDLISTLMNARDDKGQPLSDAHVLGSLRLLLIAGID
TTWSAIGAALWHLATHPAERERLLAEPELMPTAIEEFLRAYSPVTMAREVMKETSIAGCPVKPGNMVLLSFPTANRDPSV
FPEADKVMIDRKENPHVAFGLGIHRCVGSNLARMEMTVAIEEWLKRIASFRLDPSQKVRWSEGTVRGPRSLPLLFGRPS
>Mature_398_residues
SERAPVTDWVNDFDHTDPRWTENPYPIWDELRSASPLVHTDRFLGCYMPTTFAAVKEISYDTDHFSSRRVIVRNVRSESP
PPAPPITSDPPEHKPAKRLLLPPFTPDAVAKLEPRVRAICNELIDAFIEDEGCDAATAYTKHIPVKTICHMLGIPEDDSD
IFIRWIHEILELGINDDAILMKAVFEMSTYFQGHIAHRKQKPTDDLISTLMNARDDKGQPLSDAHVLGSLRLLLIAGIDT
TWSAIGAALWHLATHPAERERLLAEPELMPTAIEEFLRAYSPVTMAREVMKETSIAGCPVKPGNMVLLSFPTANRDPSVF
PEADKVMIDRKENPHVAFGLGIHRCVGSNLARMEMTVAIEEWLKRIASFRLDPSQKVRWSEGTVRGPRSLPLLFGRPS

Specific function: Hydroxylates vitamin D(3) into 25-hydroxyvitamin D(3) and 1-alpha,25-dihydroxyvitamin D(3), its physiologically active forms. It first hydroxylates the C-25 position of vitamin D(3) to form 25-hydroxyvitamin D(3), then subsequently hydroxylates the C- 1-a

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: =1.14.13.15 [H]

Molecular weight: Translated: 44864; Mature: 44733

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERAPVTDWVNDFDHTDPRWTENPYPIWDELRSASPLVHTDRFLGCYMPTTFAAVKEIS
CCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
YDTDHFSSRRVIVRNVRSESPPPAPPITSDPPEHKPAKRLLLPPFTPDAVAKLEPRVRAI
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCHHHHHH
CNELIDAFIEDEGCDAATAYTKHIPVKTICHMLGIPEDDSDIFIRWIHEILELGINDDAI
HHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHH
LMKAVFEMSTYFQGHIAHRKQKPTDDLISTLMNARDDKGQPLSDAHVLGSLRLLLIAGID
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
TTWSAIGAALWHLATHPAERERLLAEPELMPTAIEEFLRAYSPVTMAREVMKETSIAGCP
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
VKPGNMVLLSFPTANRDPSVFPEADKVMIDRKENPHVAFGLGIHRCVGSNLARMEMTVAI
CCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCEEECCHHHHHHCCCHHHHHHHHHH
EEWLKRIASFRLDPSQKVRWSEGTVRGPRSLPLLFGRPS
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCEEECCCC
>Mature Secondary Structure 
SERAPVTDWVNDFDHTDPRWTENPYPIWDELRSASPLVHTDRFLGCYMPTTFAAVKEIS
CCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
YDTDHFSSRRVIVRNVRSESPPPAPPITSDPPEHKPAKRLLLPPFTPDAVAKLEPRVRAI
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCHHHHHH
CNELIDAFIEDEGCDAATAYTKHIPVKTICHMLGIPEDDSDIFIRWIHEILELGINDDAI
HHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHH
LMKAVFEMSTYFQGHIAHRKQKPTDDLISTLMNARDDKGQPLSDAHVLGSLRLLLIAGID
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
TTWSAIGAALWHLATHPAERERLLAEPELMPTAIEEFLRAYSPVTMAREVMKETSIAGCP
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
VKPGNMVLLSFPTANRDPSVFPEADKVMIDRKENPHVAFGLGIHRCVGSNLARMEMTVAI
CCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCEEECCHHHHHHCCCHHHHHHHHHH
EEWLKRIASFRLDPSQKVRWSEGTVRGPRSLPLLFGRPS
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA