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The map label for this gene is puuB [H]

Identifier: 192290068

GI number: 192290068

Start: 1788461

End: 1789696

Strand: Reverse

Name: puuB [H]

Synonym: Rpal_1670

Alternate gene names: 192290068

Gene position: 1789696-1788461 (Counterclockwise)

Preceding gene: 192290073

Following gene: 192290060

Centisome position: 31.16

GC content: 66.26

Gene sequence:

>1236_bases
ATGGATCAGTTCCGTGCCTCTCGGCAAAGCGAACTACGCGGCGGAGTCGCGCCGTGGGCGGCCGGCCTGACACGGCCGAT
CCGCCAGCCGCTCGACCGCAATCTGGCCGTCGACGTGCTGATCGTCGGCGGCGGCATCACCGGCTCGATGATGGCCGAGT
ATCTGGCAAGGCAGGACCGCAAGGTCTGCATCATCGACCGTGAACGCCCCGGCTTCGGCAGCACGGCAGCATCGACCGCG
ATGCTGGAGTGGGAAATCGATCGGTCCTTGACTGAATTGTCGGCGTTCTACGGCTTCGAGCGTGCCGCCGACGTATATCA
GCTCAGCCACAGCGCAGTGGCGGGACTGTACCGGTTGGCCCACTCGCTGCGGCTTCCGAGCGCGCTACGACCGCGCGACA
CGGTCTATCTCGCCGCCGGAGATCACGGCGCGAAAGAGCTGCTCGAAGAGCACGAACTTCGCAAACGCGCCGGCCTGCCC
GGCGCCTTTTTGGGTTATCGCGAGCTCCGATCCGAATACGGCTTCGATCGCGAGGCGGCGATCGTCTCGCCGGGGTCGGC
CGAGGTCGATCCACTTCTGTTGTCGCACGGACTGCTGAGCGCCGCCGCAGAGCGCGGCGCCTCGCTGTTTGACGCCGAGG
CCACGGCTTACGACAGCTTCGGCCGCGGCGTGCATGTGGCAACCGACAATGGCTGCTGCATCGAAGCCGCACACGTCGTG
CTGGCGACCGGCTACATCCTACCCGAGATCGTGACCTCGGACTTGCATAAGACCGCATCGAGCTGGGTGGTGGCAACGCT
GCCGCAGCCGCCGGAGCAACTGTGGCGGGATCGCGCGCTGATCTGGGAAGCGTCGTCCGACTATCTGTACGCGCGGACCA
CGGCGGAAGACCGCATCATCATCGGTGGTGAGGACGACGATCAGGTGACCGACCCGGACGCGCGCGACGCCTTGATGCCG
GAGAAGGCGAAAATCCTGCTCGACAAGCTGCATCGGCTGTGGCCGCAGGCGGCGCCGATCCCGGAATACGTCTGGTCCGG
TGCATTTTCGACCACGGTGGACGGCCTGCCTCTGATCGGCCCAGTGCCCGGCCAGCCGCGGATCTACGCCGCCTACGGCT
ACGGCGGCAACGGAATCACCTTCAGCTATCTCGCGAGCCGATTGATCGGCCGGCTGATCGACGGCGAGCGGCTACCGGCA
CTCGACCACTTCGCACTCGACCGCTCGCCGGTCTGA

Upstream 100 bases:

>100_bases
GCACCAGCTTCAGCAATCGGAAAAGGTCTCGGCCGTGTCGGCGGTGCGAGACCTTTTTCACGATCGGCCGATCGATTTCT
GCCTCGGGAAATGATAAGCA

Downstream 100 bases:

>100_bases
GGCAACGCCCCTACGGCCCCATTGCCCGCGGATCGTTGTACATCCGCTTGATGTCCTTCTGGACGTCGAGCGGCTGCAGC
TTGCTGCTACCCTCGTCCGA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MDQFRASRQSELRGGVAPWAAGLTRPIRQPLDRNLAVDVLIVGGGITGSMMAEYLARQDRKVCIIDRERPGFGSTAASTA
MLEWEIDRSLTELSAFYGFERAADVYQLSHSAVAGLYRLAHSLRLPSALRPRDTVYLAAGDHGAKELLEEHELRKRAGLP
GAFLGYRELRSEYGFDREAAIVSPGSAEVDPLLLSHGLLSAAAERGASLFDAEATAYDSFGRGVHVATDNGCCIEAAHVV
LATGYILPEIVTSDLHKTASSWVVATLPQPPEQLWRDRALIWEASSDYLYARTTAEDRIIIGGEDDDQVTDPDARDALMP
EKAKILLDKLHRLWPQAAPIPEYVWSGAFSTTVDGLPLIGPVPGQPRIYAAYGYGGNGITFSYLASRLIGRLIDGERLPA
LDHFALDRSPV

Sequences:

>Translated_411_residues
MDQFRASRQSELRGGVAPWAAGLTRPIRQPLDRNLAVDVLIVGGGITGSMMAEYLARQDRKVCIIDRERPGFGSTAASTA
MLEWEIDRSLTELSAFYGFERAADVYQLSHSAVAGLYRLAHSLRLPSALRPRDTVYLAAGDHGAKELLEEHELRKRAGLP
GAFLGYRELRSEYGFDREAAIVSPGSAEVDPLLLSHGLLSAAAERGASLFDAEATAYDSFGRGVHVATDNGCCIEAAHVV
LATGYILPEIVTSDLHKTASSWVVATLPQPPEQLWRDRALIWEASSDYLYARTTAEDRIIIGGEDDDQVTDPDARDALMP
EKAKILLDKLHRLWPQAAPIPEYVWSGAFSTTVDGLPLIGPVPGQPRIYAAYGYGGNGITFSYLASRLIGRLIDGERLPA
LDHFALDRSPV
>Mature_411_residues
MDQFRASRQSELRGGVAPWAAGLTRPIRQPLDRNLAVDVLIVGGGITGSMMAEYLARQDRKVCIIDRERPGFGSTAASTA
MLEWEIDRSLTELSAFYGFERAADVYQLSHSAVAGLYRLAHSLRLPSALRPRDTVYLAAGDHGAKELLEEHELRKRAGLP
GAFLGYRELRSEYGFDREAAIVSPGSAEVDPLLLSHGLLSAAAERGASLFDAEATAYDSFGRGVHVATDNGCCIEAAHVV
LATGYILPEIVTSDLHKTASSWVVATLPQPPEQLWRDRALIWEASSDYLYARTTAEDRIIIGGEDDDQVTDPDARDALMP
EKAKILLDKLHRLWPQAAPIPEYVWSGAFSTTVDGLPLIGPVPGQPRIYAAYGYGGNGITFSYLASRLIGRLIDGERLPA
LDHFALDRSPV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=384, Percent_Identity=26.5625, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 44813; Mature: 44813

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQFRASRQSELRGGVAPWAAGLTRPIRQPLDRNLAVDVLIVGGGITGSMMAEYLARQDR
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCC
KVCIIDRERPGFGSTAASTAMLEWEIDRSLTELSAFYGFERAADVYQLSHSAVAGLYRLA
EEEEEECCCCCCCCHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSLRLPSALRPRDTVYLAAGDHGAKELLEEHELRKRAGLPGAFLGYRELRSEYGFDREAA
HHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCE
IVSPGSAEVDPLLLSHGLLSAAAERGASLFDAEATAYDSFGRGVHVATDNGCCIEAAHVV
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCEEEEECCCCCHHHHHHHH
LATGYILPEIVTSDLHKTASSWVVATLPQPPEQLWRDRALIWEASSDYLYARTTAEDRII
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCEEEEECCCCEEEEEECCCCEEE
IGGEDDDQVTDPDARDALMPEKAKILLDKLHRLWPQAAPIPEYVWSGAFSTTVDGLPLIG
ECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHCCCCCCCCC
PVPGQPRIYAAYGYGGNGITFSYLASRLIGRLIDGERLPALDHFALDRSPV
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MDQFRASRQSELRGGVAPWAAGLTRPIRQPLDRNLAVDVLIVGGGITGSMMAEYLARQDR
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCC
KVCIIDRERPGFGSTAASTAMLEWEIDRSLTELSAFYGFERAADVYQLSHSAVAGLYRLA
EEEEEECCCCCCCCHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSLRLPSALRPRDTVYLAAGDHGAKELLEEHELRKRAGLPGAFLGYRELRSEYGFDREAA
HHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCE
IVSPGSAEVDPLLLSHGLLSAAAERGASLFDAEATAYDSFGRGVHVATDNGCCIEAAHVV
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCEEEEECCCCCHHHHHHHH
LATGYILPEIVTSDLHKTASSWVVATLPQPPEQLWRDRALIWEASSDYLYARTTAEDRII
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCEEEEECCCCEEEEEECCCCEEE
IGGEDDDQVTDPDARDALMPEKAKILLDKLHRLWPQAAPIPEYVWSGAFSTTVDGLPLIG
ECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHCCCCCCCCC
PVPGQPRIYAAYGYGGNGITFSYLASRLIGRLIDGERLPALDHFALDRSPV
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]