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The map label for this gene is gatA [H]

Identifier: 192290053

GI number: 192290053

Start: 1773331

End: 1774707

Strand: Reverse

Name: gatA [H]

Synonym: Rpal_1654

Alternate gene names: 192290053

Gene position: 1774707-1773331 (Counterclockwise)

Preceding gene: 192290054

Following gene: 192290052

Centisome position: 30.9

GC content: 69.14

Gene sequence:

>1377_bases
ATGACCGCCTCCGAGACCGCCGCAGCTGTGGCGAACCGGACCATCACCGCTCTCGCTGCTACCGAGGCCGCGCTCGCGCG
CATCGCCAAGGCGGATGTGACGCTCAATGCCTTCACCGATGTCGTTGCCGAGCGTGCCCGCGCACGCGCACGCGAGATCG
ATGCGGCAATCGCGCGCGGCGAAAGCGTCGGCCCGCTCGCCGGCGTCCCGTTCGCGGTGAAGAATCTGTTCGACGTCGCC
GGCCTCGCCACCCGGGCCGGCTCCAAAATCAACCGCGACCGCAGCCCGGCCGCCGGCGACGCGCCGCTGATCAAGCGGCT
GGAAGCGGCTGGCGCGGTGCTGATCGGCGCGCTCAACATGGGCGAATACGCCTACGACTTCACCGGCGAGAACATCCACG
ACGGCCCGTCGCGCAATCCGCACGACCCCACCCGGATGACCGGCGGCTCCTCCGGCGGATCAGGCGCTGCGGTCGCCGGC
GGCGAGGTGCCGCTCGCGCTCGGCTCGGATACCAACGGCTCGATCCGGGTGCCGTCGTCGCTGTGCGGCATCTTCGGACT
GAAGCCGACCTATGGGCGGCTGCCGCGCACCGGCTCATTCCCGTTCGTGGCAAGCTTCGATCACCTTGGCCCGCTGGCGC
GCAGTGCCGCCGATCTGGCATTGGCCTACGACGCGATGCAGGGGCCGGACGATGGCGACGCCGCCTGTACCACGCGTGCC
TCGGAGCCCGTGTCCGGTCTGCTCGCCCAAGGCATCGACGGCCTGCGGATCGCGAAGGCTGGCGGTTACTTTGAGACTAA
TCTGTTCCCGGAAGCACGCGAAGCTGTCGATCGCGTGGTCAAGGCGCTCGGCATCACCCGCTCGGTCGAACTGCCGGAAG
CCGCCCGAGCTCGTGCCGCGGCTTACGTGATCAGCACAACCGAAGGCGCCTCGCTACATCTCGACCGGCTGCGACGACAA
CCAAACGATTTCGACCCCGCCGTGCGTGACCGGCTGCTGGCCGGCGCGATGGTGCCGGCATCGCTGGTCGATCGCGCACA
GAAATTCCGCCGCTGGTACCGCGCCCGCGTGCTCGAATTGTTCGAGCAGGTCGACGTCATCATCGCCCCGGCAACGCCTT
GCGTCGCGCCCAAGCTCGGACAGGTCACTTTCACGCTCGACGGCGTCGAGCTTCCGGTACGCGCCAACGTGGGCATCCAC
ACTCAGCCGATCTCGTTCATCGGGCTGCCAGTGGTGGCCGCCCCGATCCCGCTCGAACCGCTGCCGATCGGGGTGCAGAT
CATTGCCGCGCCGTGGCGCGAAGACATCGCGCTGCGCGTCGCCGCCGCGCTGGAACGCGCCGATGTCGCCCGCGCCCCGC
GACCGAAGGATTTCTGA

Upstream 100 bases:

>100_bases
AAGCTAGCGCGGATGGTCGATGAATTTCCGCTTCCCGATGAGACCGAGCCTGCGAGCGTGTTCCGTGCCTGAGATCGATT
CCGCCATCTCCACCGACTGG

Downstream 100 bases:

>100_bases
CCATGGATATCGATCTGCCAGAAATTGTCGCCGAAGTCAGCGAAGCCTTCACCCGCTACGAGGCCGCGCTTGTCGGCAAC
GATGTCGCCACGCTCAACGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTASETAAAVANRTITALAATEAALARIAKADVTLNAFTDVVAERARARAREIDAAIARGESVGPLAGVPFAVKNLFDVA
GLATRAGSKINRDRSPAAGDAPLIKRLEAAGAVLIGALNMGEYAYDFTGENIHDGPSRNPHDPTRMTGGSSGGSGAAVAG
GEVPLALGSDTNGSIRVPSSLCGIFGLKPTYGRLPRTGSFPFVASFDHLGPLARSAADLALAYDAMQGPDDGDAACTTRA
SEPVSGLLAQGIDGLRIAKAGGYFETNLFPEAREAVDRVVKALGITRSVELPEAARARAAAYVISTTEGASLHLDRLRRQ
PNDFDPAVRDRLLAGAMVPASLVDRAQKFRRWYRARVLELFEQVDVIIAPATPCVAPKLGQVTFTLDGVELPVRANVGIH
TQPISFIGLPVVAAPIPLEPLPIGVQIIAAPWREDIALRVAAALERADVARAPRPKDF

Sequences:

>Translated_458_residues
MTASETAAAVANRTITALAATEAALARIAKADVTLNAFTDVVAERARARAREIDAAIARGESVGPLAGVPFAVKNLFDVA
GLATRAGSKINRDRSPAAGDAPLIKRLEAAGAVLIGALNMGEYAYDFTGENIHDGPSRNPHDPTRMTGGSSGGSGAAVAG
GEVPLALGSDTNGSIRVPSSLCGIFGLKPTYGRLPRTGSFPFVASFDHLGPLARSAADLALAYDAMQGPDDGDAACTTRA
SEPVSGLLAQGIDGLRIAKAGGYFETNLFPEAREAVDRVVKALGITRSVELPEAARARAAAYVISTTEGASLHLDRLRRQ
PNDFDPAVRDRLLAGAMVPASLVDRAQKFRRWYRARVLELFEQVDVIIAPATPCVAPKLGQVTFTLDGVELPVRANVGIH
TQPISFIGLPVVAAPIPLEPLPIGVQIIAAPWREDIALRVAAALERADVARAPRPKDF
>Mature_457_residues
TASETAAAVANRTITALAATEAALARIAKADVTLNAFTDVVAERARARAREIDAAIARGESVGPLAGVPFAVKNLFDVAG
LATRAGSKINRDRSPAAGDAPLIKRLEAAGAVLIGALNMGEYAYDFTGENIHDGPSRNPHDPTRMTGGSSGGSGAAVAGG
EVPLALGSDTNGSIRVPSSLCGIFGLKPTYGRLPRTGSFPFVASFDHLGPLARSAADLALAYDAMQGPDDGDAACTTRAS
EPVSGLLAQGIDGLRIAKAGGYFETNLFPEAREAVDRVVKALGITRSVELPEAARARAAAYVISTTEGASLHLDRLRRQP
NDFDPAVRDRLLAGAMVPASLVDRAQKFRRWYRARVLELFEQVDVIIAPATPCVAPKLGQVTFTLDGVELPVRANVGIHT
QPISFIGLPVVAAPIPLEPLPIGVQIIAAPWREDIALRVAAALERADVARAPRPKDF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=25.6147540983607, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=29.9009900990099, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI166795287, Length=147, Percent_Identity=36.734693877551, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17537465, Length=497, Percent_Identity=28.5714285714286, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17543272, Length=414, Percent_Identity=28.2608695652174, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=194, Percent_Identity=31.4432989690722, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=185, Percent_Identity=29.7297297297297, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=447, Percent_Identity=24.3847874720358, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=193, Percent_Identity=31.0880829015544, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=193, Percent_Identity=31.0880829015544, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=386, Percent_Identity=28.7564766839378, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=172, Percent_Identity=33.1395348837209, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=29.8387096774194, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=29.4117647058824, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=29.4117647058824, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=29.4117647058824, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=29.4117647058824, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24644968, Length=486, Percent_Identity=26.5432098765432, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648435, Length=494, Percent_Identity=26.5182186234818, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648437, Length=494, Percent_Identity=26.5182186234818, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45550774, Length=494, Percent_Identity=26.5182186234818, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=26.6019417475728, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648441, Length=434, Percent_Identity=25.8064516129032, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648439, Length=434, Percent_Identity=25.8064516129032, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356731, Length=228, Percent_Identity=33.7719298245614, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47894; Mature: 47763

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASETAAAVANRTITALAATEAALARIAKADVTLNAFTDVVAERARARAREIDAAIARG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ESVGPLAGVPFAVKNLFDVAGLATRAGSKINRDRSPAAGDAPLIKRLEAAGAVLIGALNM
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECC
GEYAYDFTGENIHDGPSRNPHDPTRMTGGSSGGSGAAVAGGEVPLALGSDTNGSIRVPSS
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCEECCHH
LCGIFGLKPTYGRLPRTGSFPFVASFDHLGPLARSAADLALAYDAMQGPDDGDAACTTRA
HHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SEPVSGLLAQGIDGLRIAKAGGYFETNLFPEAREAVDRVVKALGITRSVELPEAARARAA
CCHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEE
AYVISTTEGASLHLDRLRRQPNDFDPAVRDRLLAGAMVPASLVDRAQKFRRWYRARVLEL
EEEEECCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
FEQVDVIIAPATPCVAPKLGQVTFTLDGVELPVRANVGIHTQPISFIGLPVVAAPIPLEP
HHHCCEEEECCCCCCCCCCCCEEEEECCEEECEECCCCCEECCHHHHCCCHHCCCCCCCC
LPIGVQIIAAPWREDIALRVAAALERADVARAPRPKDF
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TASETAAAVANRTITALAATEAALARIAKADVTLNAFTDVVAERARARAREIDAAIARG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ESVGPLAGVPFAVKNLFDVAGLATRAGSKINRDRSPAAGDAPLIKRLEAAGAVLIGALNM
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECC
GEYAYDFTGENIHDGPSRNPHDPTRMTGGSSGGSGAAVAGGEVPLALGSDTNGSIRVPSS
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCEECCHH
LCGIFGLKPTYGRLPRTGSFPFVASFDHLGPLARSAADLALAYDAMQGPDDGDAACTTRA
HHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SEPVSGLLAQGIDGLRIAKAGGYFETNLFPEAREAVDRVVKALGITRSVELPEAARARAA
CCHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEE
AYVISTTEGASLHLDRLRRQPNDFDPAVRDRLLAGAMVPASLVDRAQKFRRWYRARVLEL
EEEEECCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
FEQVDVIIAPATPCVAPKLGQVTFTLDGVELPVRANVGIHTQPISFIGLPVVAAPIPLEP
HHHCCEEEECCCCCCCCCCCCEEEEECCEEECEECCCCCEECCHHHHCCCHHCCCCCCCC
LPIGVQIIAAPWREDIALRVAAALERADVARAPRPKDF
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA