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The map label for this gene is anfD [H]

Identifier: 192290021

GI number: 192290021

Start: 1735753

End: 1737321

Strand: Reverse

Name: anfD [H]

Synonym: Rpal_1622

Alternate gene names: 192290021

Gene position: 1737321-1735753 (Counterclockwise)

Preceding gene: 192290022

Following gene: 192290020

Centisome position: 30.25

GC content: 61.89

Gene sequence:

>1569_bases
ATGCCATATCACGAGTTCGACTGTTCGAAATGTCTGCCCGAACGCCAGAAGCACGCCGTCACCAAGGGCGCCGGCGATAA
TCTCGCCACCGCGCTGCCGCTCGGCTATCTCAACACGATCCCGGGATCGATCTCGGAGCGCGGCTGCGCCTATTGCGGCG
CAAAGCATGTGATCGGCACGCCGATGAAGGACGTGATTCACATGAGTCACGGCCCCGTCGGCTGCACCTACGACACCTGG
CAGACCAAGCGCTATATCTCCGACAACAACAACTTCCAGCTCAAATACACCTTCGCCACCGACGTCCGCGAGAAGCACAT
CGTATTCGGCGCCGAAGGCCTTCTGAAGCAGAACATCATCGAGGCTTTCAAAGCCTGCCCCGACATCAAGCGGATGACGA
TCTACCAGACCTGCGCCACCGCGCTGATCGGCGACGACATCAATGCTGTCGCCGCCGAGGTGATGGAGGAGATGCCGGAC
GTCGACATCTTCACCTGCAACTCGCCAGGCTTCGGCGGCCCCAGCCAGTCCGGCGGCCACCACAAGATCAACATCGCCTG
GATCAACGACAAGGTCGGCACCGTCGAGCCCGAGATCACCTCGGATTACGTCATCAACTATGTGGGCGAATACAACATCC
AGGGCGACCAGGAGGTGATGCTCGACTATTTCACCCGGATGGGTATCCAGGTGCTGTCGACCTTCACCGGCAATGGCACC
TATGACGGCCTGCGGGCGATGCACCGCGCGCATCTCAACGTGCTCGAATGCGCCCGTTCGGCCGAATACATCTGCAACGA
GCTGCGCGTCCGCTACGGGATTCCGCGGCTCGACATCGACGGCTTCGGCTTCGAGCCGTTGTCGCAGTCGCTGCGCAAGA
TCGGGATGTTCTTCGGCATCGAAGACCGCGCCGAAGCGATCATCGCCGAAGAGACCGCGCGCTGGAAGCCGGAGCTCGAC
TGGTACAAGGAACGGCTGAAGGGCAAAAAGGTCTGCCTGTGGCCAGGCGGCTCCAAGCTGTGGCACTGGGCGCACGCCAT
CGAAGAGGAGATGGGCGTCAAGGTCGTCTCGGTCTACACTAAGTTCGGCCACCAGGGCGACATGGAAAAGGGCATCGCGC
GCTGCGGCGAGGATGCGCTGGCGATCGACGATCCCAACGAACTCGAGGGCCTCGAGGCGCTGGAGAAGCTGCAGCCGGAC
ATCATCTTCACCGGCAAGCGTCCCGGCGAAGTCGCCAAGAAGGTCCGCGTTCCGTACCTCAACGCCCACGCCTATCACAA
CGGCCCATACAAGGGCTTCGAAGGCTGGGTGCGGTTCGCCCGCGACATCTACAACGGCATCTACTCGCCGATGCACCAGC
TCTCCGGGCTGGACATCAGCAAGGACGAGATTCCGGCCGATCGCGGTTTCGTCACGCAGCGCATGCTGTCCGACGCGAAG
CTGCCGGAAGAGATCGCCAAGTCGGAGACGCTGCGGCGCTACACCGGCAAGGACGACATCATCTCCGACCTGCGCAAGAA
GAACGCGCCCTACTTCACCCCGATCGTCAAAGCCGAAGCGGCCGAGTGA

Upstream 100 bases:

>100_bases
ACGATGGATCAGCTCGAAGCGATGGTCGTGAAGTACGGCCTGATGGACTGACCTGCGCTCCCCGCGCCTGACGGCGTACT
TCCCGCACGAGGACAATACA

Downstream 100 bases:

>100_bases
GACGAGAGCCCATGACCAAGATCACCTACAAAGTCGCCTCGGAACATCCGTTCCCCGCCGACGCCGCCGCGGCGGCCGCC
TCGCTCGCGGCCTATGAGGC

Product: nitrogenase alpha chain

Products: NA

Alternate protein names: Dinitrogenase 3 subunit alpha; Nitrogenase component I [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MPYHEFDCSKCLPERQKHAVTKGAGDNLATALPLGYLNTIPGSISERGCAYCGAKHVIGTPMKDVIHMSHGPVGCTYDTW
QTKRYISDNNNFQLKYTFATDVREKHIVFGAEGLLKQNIIEAFKACPDIKRMTIYQTCATALIGDDINAVAAEVMEEMPD
VDIFTCNSPGFGGPSQSGGHHKINIAWINDKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFTRMGIQVLSTFTGNGT
YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSQSLRKIGMFFGIEDRAEAIIAEETARWKPELD
WYKERLKGKKVCLWPGGSKLWHWAHAIEEEMGVKVVSVYTKFGHQGDMEKGIARCGEDALAIDDPNELEGLEALEKLQPD
IIFTGKRPGEVAKKVRVPYLNAHAYHNGPYKGFEGWVRFARDIYNGIYSPMHQLSGLDISKDEIPADRGFVTQRMLSDAK
LPEEIAKSETLRRYTGKDDIISDLRKKNAPYFTPIVKAEAAE

Sequences:

>Translated_522_residues
MPYHEFDCSKCLPERQKHAVTKGAGDNLATALPLGYLNTIPGSISERGCAYCGAKHVIGTPMKDVIHMSHGPVGCTYDTW
QTKRYISDNNNFQLKYTFATDVREKHIVFGAEGLLKQNIIEAFKACPDIKRMTIYQTCATALIGDDINAVAAEVMEEMPD
VDIFTCNSPGFGGPSQSGGHHKINIAWINDKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFTRMGIQVLSTFTGNGT
YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSQSLRKIGMFFGIEDRAEAIIAEETARWKPELD
WYKERLKGKKVCLWPGGSKLWHWAHAIEEEMGVKVVSVYTKFGHQGDMEKGIARCGEDALAIDDPNELEGLEALEKLQPD
IIFTGKRPGEVAKKVRVPYLNAHAYHNGPYKGFEGWVRFARDIYNGIYSPMHQLSGLDISKDEIPADRGFVTQRMLSDAK
LPEEIAKSETLRRYTGKDDIISDLRKKNAPYFTPIVKAEAAE
>Mature_521_residues
PYHEFDCSKCLPERQKHAVTKGAGDNLATALPLGYLNTIPGSISERGCAYCGAKHVIGTPMKDVIHMSHGPVGCTYDTWQ
TKRYISDNNNFQLKYTFATDVREKHIVFGAEGLLKQNIIEAFKACPDIKRMTIYQTCATALIGDDINAVAAEVMEEMPDV
DIFTCNSPGFGGPSQSGGHHKINIAWINDKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFTRMGIQVLSTFTGNGTY
DGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSQSLRKIGMFFGIEDRAEAIIAEETARWKPELDW
YKERLKGKKVCLWPGGSKLWHWAHAIEEEMGVKVVSVYTKFGHQGDMEKGIARCGEDALAIDDPNELEGLEALEKLQPDI
IFTGKRPGEVAKKVRVPYLNAHAYHNGPYKGFEGWVRFARDIYNGIYSPMHQLSGLDISKDEIPADRGFVTQRMLSDAKL
PEEIAKSETLRRYTGKDDIISDLRKKNAPYFTPIVKAEAAE

Specific function: This iron-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation. Other nitrogenase complexes utilize a molybdenum-iron protein or a vanadium-iron protein [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR005974
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR011290 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 58542; Mature: 58411

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYHEFDCSKCLPERQKHAVTKGAGDNLATALPLGYLNTIPGSISERGCAYCGAKHVIGT
CCCCCCCHHHHCHHHHHHHHHCCCCCCHHHHHCHHHHHHCCCCCCCCCHHHHCCCHHCCC
PMKDVIHMSHGPVGCTYDTWQTKRYISDNNNFQLKYTFATDVREKHIVFGAEGLLKQNII
CHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHH
EAFKACPDIKRMTIYQTCATALIGDDINAVAAEVMEEMPDVDIFTCNSPGFGGPSQSGGH
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
HKINIAWINDKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFTRMGIQVLSTFTGNGT
EEEEEEEECCCCCCCCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSQSLRKIGMFFGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCC
EDRAEAIIAEETARWKPELDWYKERLKGKKVCLWPGGSKLWHWAHAIEEEMGVKVVSVYT
CHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEHHH
KFGHQGDMEKGIARCGEDALAIDDPNELEGLEALEKLQPDIIFTGKRPGEVAKKVRVPYL
HHCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCEE
NAHAYHNGPYKGFEGWVRFARDIYNGIYSPMHQLSGLDISKDEIPADRGFVTQRMLSDAK
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHCC
LPEEIAKSETLRRYTGKDDIISDLRKKNAPYFTPIVKAEAAE
CHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PYHEFDCSKCLPERQKHAVTKGAGDNLATALPLGYLNTIPGSISERGCAYCGAKHVIGT
CCCCCCHHHHCHHHHHHHHHCCCCCCHHHHHCHHHHHHCCCCCCCCCHHHHCCCHHCCC
PMKDVIHMSHGPVGCTYDTWQTKRYISDNNNFQLKYTFATDVREKHIVFGAEGLLKQNII
CHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHH
EAFKACPDIKRMTIYQTCATALIGDDINAVAAEVMEEMPDVDIFTCNSPGFGGPSQSGGH
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
HKINIAWINDKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFTRMGIQVLSTFTGNGT
EEEEEEEECCCCCCCCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSQSLRKIGMFFGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCC
EDRAEAIIAEETARWKPELDWYKERLKGKKVCLWPGGSKLWHWAHAIEEEMGVKVVSVYT
CHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEHHH
KFGHQGDMEKGIARCGEDALAIDDPNELEGLEALEKLQPDIIFTGKRPGEVAKKVRVPYL
HHCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCEE
NAHAYHNGPYKGFEGWVRFARDIYNGIYSPMHQLSGLDISKDEIPADRGFVTQRMLSDAK
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHCC
LPEEIAKSETLRRYTGKDDIISDLRKKNAPYFTPIVKAEAAE
CHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644222; 8392330 [H]