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The map label for this gene is anfK [H]

Identifier: 192290019

GI number: 192290019

Start: 1733902

End: 1735287

Strand: Reverse

Name: anfK [H]

Synonym: Rpal_1620

Alternate gene names: 192290019

Gene position: 1735287-1733902 (Counterclockwise)

Preceding gene: 192290020

Following gene: 192290018

Centisome position: 30.21

GC content: 62.27

Gene sequence:

>1386_bases
ATGAACTGTCAATTCAAGCCGAAGGATCGGATCGGCACCATCAATCCGATCTTCACCTGCCAGCCGGCCGGCGCCCAATA
CGCCTCGATCGGTATCAAGGACTGCATCGGCATCGTCCACGGCGGCCAGGGCTGCGTGATGTTCGTCCGCCTGCTGATCT
CGCAGCACCTCAAAGAGAGCTTCGAGATCGCCTCCTCGTCGGTGCACGAGGACGGCGCAGTGTTCGGCGCACTCGACCGC
GTCGAGGAAGCGGTCGACGTGCTGCTGATGCGCTACCCGCACGTCAAGGTGGTGCCGATCATCACCACCTGCTCGACCGA
AGTAATCGGCGACGACGTCGACGGCGTCGTCACCAAGCTGCAGGAAGAGCTGCTCGACGTGAAATATCCGGACCGCGAGG
TCCATCTGATCCCGATCCACTGCCCGAGCTTCGTCGGCAGTATGGTGACGGGCTACGACGTCGCCGTGCGCGACTTCGTC
AAGCATTTCTCCAAGAAGGACAAACCGAGCCTGAAGATCAATCTGATCACCGGCTGGGTCAATCCGGGCGACGTCAAGGA
GCTCAAGCATCTGCTCGCCGAGATGCAGGTCGAAGCCAACGTGCTGTTCGAGATCGAGAGCTTCGATAGCCCGCTGATGC
CGGACAAGAGCGCGGTGTCGCACGGCTCGACCACGATTGCGGACCTGACCGCAACCGCCGATGCGCAAGGCACCATCGCA
CTCAACCGCTACGAAGGTGGCCTCGCCGCCACCTGGCTGCAGACCCGCTGGGACGTGCCGGCAGTGATCGGCTCAACCCC
GATCGGGATCCGCAACACCGACACCTTCCTGCGCAACGTCAAGGACATGACAGGCAAGCCGATCCCGGAATCGCTGGTCA
AGGAACGCGGCATCGCGCTCGACGCCATCACCGACCTCGCCCACATGTTCCTGGCCGACAAGAAGGTGGCGATCTACGGC
AATCCGGATCTGGTGCTCGGCCTCGCCGAGTACTGCCTCGACCTGGAAATGAAACCGGTGCTGCTGCTGCTCGGCGACGA
GAACGCCGGCTACGCCAACGACCCTCGGATCGCGGCGCTGAAGGCCAATGTCGACTACCCGATGGAGATCATCACCAACG
CCGACCTGTTCGAGCTCGAAGAGCGCATCAAGAACGGCCTCGAGCTCGATCTGATCCTCGGCCACTCCAAGGGACGGTTC
GTCGCGATCGACTACAACATCCCGATGCTACGGGTCGGCTTTCCGACCTACGACCGCGCCGGCCTGTATCGCTACCCCGT
GGTCGGCTACGCCGGCGCGACCTGGCTCGCCGAACAGATGGCCAACACGCTGTTCAACGACATGGAGGTCAAGAAGAACC
GCGAATGGATCCTGAACGTGTGGTGA

Upstream 100 bases:

>100_bases
GCGCTGATGCAAGAGCTGCACGCCCGCGTCGACTATCTCACCGTCACCGGCTCGCTCAACGAAGAGCTCACCGACCTGCA
CTACTGATTGGACCCCCGCC

Downstream 100 bases:

>100_bases
CCACGCTCAAGATCTGGTCCGGCGACGGTTTGCCATGTCCGTCGCCGGACTCTTCTTTCTCCTGACGGCCGCGAGACAAC
CGATGAAGATCGCAGCTTAT

Product: Fe-only nitrogenase subunit beta

Products: NA

Alternate protein names: Dinitrogenase 3 subunit beta; Nitrogenase component I [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNCQFKPKDRIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKESFEIASSSVHEDGAVFGALDR
VEEAVDVLLMRYPHVKVVPIITTCSTEVIGDDVDGVVTKLQEELLDVKYPDREVHLIPIHCPSFVGSMVTGYDVAVRDFV
KHFSKKDKPSLKINLITGWVNPGDVKELKHLLAEMQVEANVLFEIESFDSPLMPDKSAVSHGSTTIADLTATADAQGTIA
LNRYEGGLAATWLQTRWDVPAVIGSTPIGIRNTDTFLRNVKDMTGKPIPESLVKERGIALDAITDLAHMFLADKKVAIYG
NPDLVLGLAEYCLDLEMKPVLLLLGDENAGYANDPRIAALKANVDYPMEIITNADLFELEERIKNGLELDLILGHSKGRF
VAIDYNIPMLRVGFPTYDRAGLYRYPVVGYAGATWLAEQMANTLFNDMEVKKNREWILNVW

Sequences:

>Translated_461_residues
MNCQFKPKDRIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKESFEIASSSVHEDGAVFGALDR
VEEAVDVLLMRYPHVKVVPIITTCSTEVIGDDVDGVVTKLQEELLDVKYPDREVHLIPIHCPSFVGSMVTGYDVAVRDFV
KHFSKKDKPSLKINLITGWVNPGDVKELKHLLAEMQVEANVLFEIESFDSPLMPDKSAVSHGSTTIADLTATADAQGTIA
LNRYEGGLAATWLQTRWDVPAVIGSTPIGIRNTDTFLRNVKDMTGKPIPESLVKERGIALDAITDLAHMFLADKKVAIYG
NPDLVLGLAEYCLDLEMKPVLLLLGDENAGYANDPRIAALKANVDYPMEIITNADLFELEERIKNGLELDLILGHSKGRF
VAIDYNIPMLRVGFPTYDRAGLYRYPVVGYAGATWLAEQMANTLFNDMEVKKNREWILNVW
>Mature_461_residues
MNCQFKPKDRIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKESFEIASSSVHEDGAVFGALDR
VEEAVDVLLMRYPHVKVVPIITTCSTEVIGDDVDGVVTKLQEELLDVKYPDREVHLIPIHCPSFVGSMVTGYDVAVRDFV
KHFSKKDKPSLKINLITGWVNPGDVKELKHLLAEMQVEANVLFEIESFDSPLMPDKSAVSHGSTTIADLTATADAQGTIA
LNRYEGGLAATWLQTRWDVPAVIGSTPIGIRNTDTFLRNVKDMTGKPIPESLVKERGIALDAITDLAHMFLADKKVAIYG
NPDLVLGLAEYCLDLEMKPVLLLLGDENAGYANDPRIAALKANVDYPMEIITNADLFELEERIKNGLELDLILGHSKGRF
VAIDYNIPMLRVGFPTYDRAGLYRYPVVGYAGATWLAEQMANTLFNDMEVKKNREWILNVW

Specific function: This iron-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation. Other nitrogenase complexes utilize a molybdenum-iron protein or a vanadium-iron protein [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR014280 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50947; Mature: 50947

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCQFKPKDRIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES
CCCCCCCCCCCCCCCCEEEECCCCCCHHHCCHHHHHHHEECCCHHHHHHHHHHHHHHHHH
FEIASSSVHEDGAVFGALDRVEEAVDVLLMRYPHVKVVPIITTCSTEVIGDDVDGVVTKL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHCCCCHHHHHHHH
QEELLDVKYPDREVHLIPIHCPSFVGSMVTGYDVAVRDFVKHFSKKDKPSLKINLITGWV
HHHHHCCCCCCCEEEEEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEECC
NPGDVKELKHLLAEMQVEANVLFEIESFDSPLMPDKSAVSHGSTTIADLTATADAQGTIA
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEE
LNRYEGGLAATWLQTRWDVPAVIGSTPIGIRNTDTFLRNVKDMTGKPIPESLVKERGIAL
EEECCCCEEEEEHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCH
DAITDLAHMFLADKKVAIYGNPDLVLGLAEYCLDLEMKPVLLLLGDENAGYANDPRIAAL
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEE
KANVDYPMEIITNADLFELEERIKNGLELDLILGHSKGRFVAIDYNIPMLRVGFPTYDRA
EECCCCCHHHHCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCEEEECCCCCCCC
GLYRYPVVGYAGATWLAEQMANTLFNDMEVKKNREWILNVW
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MNCQFKPKDRIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES
CCCCCCCCCCCCCCCCEEEECCCCCCHHHCCHHHHHHHEECCCHHHHHHHHHHHHHHHHH
FEIASSSVHEDGAVFGALDRVEEAVDVLLMRYPHVKVVPIITTCSTEVIGDDVDGVVTKL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHCCCCHHHHHHHH
QEELLDVKYPDREVHLIPIHCPSFVGSMVTGYDVAVRDFVKHFSKKDKPSLKINLITGWV
HHHHHCCCCCCCEEEEEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEECC
NPGDVKELKHLLAEMQVEANVLFEIESFDSPLMPDKSAVSHGSTTIADLTATADAQGTIA
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEE
LNRYEGGLAATWLQTRWDVPAVIGSTPIGIRNTDTFLRNVKDMTGKPIPESLVKERGIAL
EEECCCCEEEEEHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCH
DAITDLAHMFLADKKVAIYGNPDLVLGLAEYCLDLEMKPVLLLLGDENAGYANDPRIAAL
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEE
KANVDYPMEIITNADLFELEERIKNGLELDLILGHSKGRFVAIDYNIPMLRVGFPTYDRA
EECCCCCHHHHCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCEEEECCCCCCCC
GLYRYPVVGYAGATWLAEQMANTLFNDMEVKKNREWILNVW
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644222; 8392330 [H]