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The map label for this gene is rob [C]

Identifier: 192289947

GI number: 192289947

Start: 1628986

End: 1629888

Strand: Reverse

Name: rob [C]

Synonym: Rpal_1543

Alternate gene names: 192289947

Gene position: 1629888-1628986 (Counterclockwise)

Preceding gene: 192289965

Following gene: 192289943

Centisome position: 28.38

GC content: 61.46

Gene sequence:

>903_bases
ATGACCAAACTGGAGGCGTTCGGGGATCGAAAGTTTCCAGCCGCCGAGCTTATTCTAAGCTCCGAAGGACGAGGCTGGGA
CGGCCTCGGCACCCAGGTTCGGCGCCATCCCGCCGGCGAGATCCCGGTGCCGATTCCCGACCAGATGGAGGTCACCCTCG
CAATCGCCGGCTCACAGACTGGGCGCGTGGAGCGTCGCTCCGGTAGCGCGTTTCAGAATACGACGGCGCGACCGGGCACG
CTGTGGTTCTGTCCCATTGGCGTCACCGAGGATTCGATCCGGATCACCGAAACGCTGCCTGTGGTGCAGCACGTCTTCCT
TGCAAAGTCGCAGTTCGACAGTCTTTCCGAGCTGAGTTCGAGATCGCTGTCGCCTGCGAGCATCGCCTATCTAGCGGACG
TCAACGACGAGTTGGTCCGCCAGATCTGCCAGCGGATCTATCGGGAGGCGCAGGAAGAAACCAGCAGCGGCAAGATCCTG
ATCGACTGCCTCGCCCAGTCCCTGGTGGCGCATCTCGTTTCCGACTATTCCCACGACAGCCGTATCCACCTCAGCCCCGA
CAGCGGACCATCGAAGCTCGACGACGCGAGGCTGCGCCGGGTGCTGGACTACATTTACGACAACATCGACCAGGATCTGT
CTCTGTCGAAGCTGTCGGACGTCGCCTGCCTCAGCAAACATCATTTCGCACGGGCGTTCCGGGAAGCGACGGGAACGCCG
CCCTACCGCTTCATCAGTGAGCGAAGACTTGATCACGCGCTGAACCTGCTCAAAGACGGCCAACTGAGCTTGGCGGAAAT
CGCCTACATCTGCCGGTTCTCCTCACAGGCAACTTTCACCCGGGCGTTTCGGCGCCATGTCGGCACCTCGCCAGGTGAAT
ATCGACATCTGTCCCGCACCTGA

Upstream 100 bases:

>100_bases
CGTTTTGGCCAGAATTGATATGCTTGATACGGCTGAGGCCAGGGTCCAGATTGCCAAGAGGCGACGGGATGCAACCGATG
AGCCTGAGGACGAGGAACAG

Downstream 100 bases:

>100_bases
GCGCGGGGCGTCGGCCTAGATGCATCGCCGCCATGCTACTCCGCTGGCGGCGGCACGATTGGCTTCGCGCCGGCTGGAGC
GCTGTTCGGCAGGATCGACA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTKLEAFGDRKFPAAELILSSEGRGWDGLGTQVRRHPAGEIPVPIPDQMEVTLAIAGSQTGRVERRSGSAFQNTTARPGT
LWFCPIGVTEDSIRITETLPVVQHVFLAKSQFDSLSELSSRSLSPASIAYLADVNDELVRQICQRIYREAQEETSSGKIL
IDCLAQSLVAHLVSDYSHDSRIHLSPDSGPSKLDDARLRRVLDYIYDNIDQDLSLSKLSDVACLSKHHFARAFREATGTP
PYRFISERRLDHALNLLKDGQLSLAEIAYICRFSSQATFTRAFRRHVGTSPGEYRHLSRT

Sequences:

>Translated_300_residues
MTKLEAFGDRKFPAAELILSSEGRGWDGLGTQVRRHPAGEIPVPIPDQMEVTLAIAGSQTGRVERRSGSAFQNTTARPGT
LWFCPIGVTEDSIRITETLPVVQHVFLAKSQFDSLSELSSRSLSPASIAYLADVNDELVRQICQRIYREAQEETSSGKIL
IDCLAQSLVAHLVSDYSHDSRIHLSPDSGPSKLDDARLRRVLDYIYDNIDQDLSLSKLSDVACLSKHHFARAFREATGTP
PYRFISERRLDHALNLLKDGQLSLAEIAYICRFSSQATFTRAFRRHVGTSPGEYRHLSRT
>Mature_299_residues
TKLEAFGDRKFPAAELILSSEGRGWDGLGTQVRRHPAGEIPVPIPDQMEVTLAIAGSQTGRVERRSGSAFQNTTARPGTL
WFCPIGVTEDSIRITETLPVVQHVFLAKSQFDSLSELSSRSLSPASIAYLADVNDELVRQICQRIYREAQEETSSGKILI
DCLAQSLVAHLVSDYSHDSRIHLSPDSGPSKLDDARLRRVLDYIYDNIDQDLSLSKLSDVACLSKHHFARAFREATGTPP
YRFISERRLDHALNLLKDGQLSLAEIAYICRFSSQATFTRAFRRHVGTSPGEYRHLSRT

Specific function: Binds To The Right Arm Of The Replication Origin Oric Of The E.Coli Chromosome. Rob Binding May Influence The Formation Of The Nucleoprotein Structure, Required For Oric Function In The Initiation Of Replication. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081928, Length=102, Percent_Identity=32.3529411764706, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33473; Mature: 33342

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLEAFGDRKFPAAELILSSEGRGWDGLGTQVRRHPAGEIPVPIPDQMEVTLAIAGSQT
CCCCCCCCCCCCCHHHHEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
GRVERRSGSAFQNTTARPGTLWFCPIGVTEDSIRITETLPVVQHVFLAKSQFDSLSELSS
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RSLSPASIAYLADVNDELVRQICQRIYREAQEETSSGKILIDCLAQSLVAHLVSDYSHDS
CCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
RIHLSPDSGPSKLDDARLRRVLDYIYDNIDQDLSLSKLSDVACLSKHHFARAFREATGTP
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PYRFISERRLDHALNLLKDGQLSLAEIAYICRFSSQATFTRAFRRHVGTSPGEYRHLSRT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKLEAFGDRKFPAAELILSSEGRGWDGLGTQVRRHPAGEIPVPIPDQMEVTLAIAGSQT
CCCCCCCCCCCCHHHHEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
GRVERRSGSAFQNTTARPGTLWFCPIGVTEDSIRITETLPVVQHVFLAKSQFDSLSELSS
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RSLSPASIAYLADVNDELVRQICQRIYREAQEETSSGKILIDCLAQSLVAHLVSDYSHDS
CCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
RIHLSPDSGPSKLDDARLRRVLDYIYDNIDQDLSLSKLSDVACLSKHHFARAFREATGTP
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PYRFISERRLDHALNLLKDGQLSLAEIAYICRFSSQATFTRAFRRHVGTSPGEYRHLSRT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]