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The map label for this gene is cusB [H]

Identifier: 192289471

GI number: 192289471

Start: 1115328

End: 1116626

Strand: Reverse

Name: cusB [H]

Synonym: Rpal_1046

Alternate gene names: 192289471

Gene position: 1116626-1115328 (Counterclockwise)

Preceding gene: 192289472

Following gene: 192289470

Centisome position: 19.44

GC content: 60.43

Gene sequence:

>1299_bases
GTGTCGACGAAACGATATCGGAAGTCGATCGGCCCTGCCCTGGCCCAACTGATGGTCTTGTGCGCGGGCTACTTCATCAG
CCAAGACGCGAGTTCAGCACAACAGGCCCCCGAGCCCCAGCAGAACATGCCGGCCGATCACTCCGTGCCCGGCAATTCGA
TGCCTCCGGGATATACGGAACTAAGCGTCGATCCGGCAATCCGGCAGCGTATCGGAGTTACGCTGGGAAGGGCGACGCGG
ACCCCCCTCACCATGACCATTCGAACGGTCGGGATCGTACGTCCCGATGAAACCAAGCTTGCTCATATTCACCTCAAGAC
CGAAGGCTGGGTCGAACGACTGTTCGTCTCTTTCACCGGGCAACAGGTGAAAGCCGGCGAAGCCATGCTTGCGATCTACA
GTCCGGCCTTCTTTGCGGCCCAGAGAGAGCTGTTGTCCGCGGCGCGGTCGGCACAATCCAATCTCGGAGGCATGACAGAT
CAACAGCTGGTGTTGGAAGCCGCGCGTCGACGCCTGGAGCTTTGGGATGTGCCTGCCGATGCGATCAAGGCCCTGGAGGC
GACCGGGCGGCCCGGGACGTCGTTGGTACTACGCAGTCCAATCTCAGGTACCGTCCTGGAGAAGAAGGTCTTTCAAGGTC
AATACACGATGCCGCAGAACGAACTGTTCGTCGTCGCCGACCTCTCGACGGTGTGGGTTCAGGGCAAGGTCTTCGAGTAT
GAATTGCCGCACGTCCAGATCGGCATGGCTGTGACTGTCGCGCTGCCCTCGTCGCCGACGCAACAATATACCGGCAAGGT
CGTGTTCATCGATCCGGTGGTCGACGAGATGACCCGATCGGTGCAAGTCCGCGTCGAGCTACCAAATCCCGACGGCCGAC
TGAAGCCAGGCATGTTCAGTCAGATCTTGATCAGCCACGCGATGGGAAGCGGCTTGACCGTTCCGGTATCGGCCGTCATC
CGCACCGGCGAACGCAACATCGCGTTCCGAGCTGTTGCCGATGATCGCTTTCTCCCGGTCCAAGTGAAAATCAGCCCGCT
GCGGTTCGAGGATCGATTCCAGATCCTGGAGGGGCTGGAGGATGGCGACATGGTTGCCACTTCCGCCAACTTCTTGATCG
ATTCCGAAAGCCAGCTACTGGCCGGCGGCGGTGGTATGGCCGGCATGCCCGGGATGGACGACGGCAGCAAGGCCAACGAG
AACAAACCGGCAGCCCGGCCGCAGGACGTCAAGGGGATGTCGATGCCAAATCGACCTGCCGCAGACGATCATTCCACCAT
GAAACACGATCGTCCGTGA

Upstream 100 bases:

>100_bases
GAGGCGCTTGCGGGTGTCGACATCGAGACACTTGGAACGTCGCCACCCGGAGCGGCCTCGCTGGCCGCACAATAGAAAGT
CCAGCACCGTGAGGGATCTC

Downstream 100 bases:

>100_bases
GCGAAAGATAGACCATGATCGGCCGGCTGATCGAGTTCAGCGCGCGGAACGCGCTTCTCATGCTTCTCCTTATCGTGGCC
ATCATCGGCGGAGGCGTTTG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSTKRYRKSIGPALAQLMVLCAGYFISQDASSAQQAPEPQQNMPADHSVPGNSMPPGYTELSVDPAIRQRIGVTLGRATR
TPLTMTIRTVGIVRPDETKLAHIHLKTEGWVERLFVSFTGQQVKAGEAMLAIYSPAFFAAQRELLSAARSAQSNLGGMTD
QQLVLEAARRRLELWDVPADAIKALEATGRPGTSLVLRSPISGTVLEKKVFQGQYTMPQNELFVVADLSTVWVQGKVFEY
ELPHVQIGMAVTVALPSSPTQQYTGKVVFIDPVVDEMTRSVQVRVELPNPDGRLKPGMFSQILISHAMGSGLTVPVSAVI
RTGERNIAFRAVADDRFLPVQVKISPLRFEDRFQILEGLEDGDMVATSANFLIDSESQLLAGGGGMAGMPGMDDGSKANE
NKPAARPQDVKGMSMPNRPAADDHSTMKHDRP

Sequences:

>Translated_432_residues
MSTKRYRKSIGPALAQLMVLCAGYFISQDASSAQQAPEPQQNMPADHSVPGNSMPPGYTELSVDPAIRQRIGVTLGRATR
TPLTMTIRTVGIVRPDETKLAHIHLKTEGWVERLFVSFTGQQVKAGEAMLAIYSPAFFAAQRELLSAARSAQSNLGGMTD
QQLVLEAARRRLELWDVPADAIKALEATGRPGTSLVLRSPISGTVLEKKVFQGQYTMPQNELFVVADLSTVWVQGKVFEY
ELPHVQIGMAVTVALPSSPTQQYTGKVVFIDPVVDEMTRSVQVRVELPNPDGRLKPGMFSQILISHAMGSGLTVPVSAVI
RTGERNIAFRAVADDRFLPVQVKISPLRFEDRFQILEGLEDGDMVATSANFLIDSESQLLAGGGGMAGMPGMDDGSKANE
NKPAARPQDVKGMSMPNRPAADDHSTMKHDRP
>Mature_431_residues
STKRYRKSIGPALAQLMVLCAGYFISQDASSAQQAPEPQQNMPADHSVPGNSMPPGYTELSVDPAIRQRIGVTLGRATRT
PLTMTIRTVGIVRPDETKLAHIHLKTEGWVERLFVSFTGQQVKAGEAMLAIYSPAFFAAQRELLSAARSAQSNLGGMTDQ
QLVLEAARRRLELWDVPADAIKALEATGRPGTSLVLRSPISGTVLEKKVFQGQYTMPQNELFVVADLSTVWVQGKVFEYE
LPHVQIGMAVTVALPSSPTQQYTGKVVFIDPVVDEMTRSVQVRVELPNPDGRLKPGMFSQILISHAMGSGLTVPVSAVIR
TGERNIAFRAVADDRFLPVQVKISPLRFEDRFQILEGLEDGDMVATSANFLIDSESQLLAGGGGMAGMPGMDDGSKANEN
KPAARPQDVKGMSMPNRPAADDHSTMKHDRP

Specific function: Part of a cation efflux system that mediates resistance to copper and silver [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=324, Percent_Identity=28.3950617283951, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46921; Mature: 46790

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKRYRKSIGPALAQLMVLCAGYFISQDASSAQQAPEPQQNMPADHSVPGNSMPPGYTE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCCCEE
LSVDPAIRQRIGVTLGRATRTPLTMTIRTVGIVRPDETKLAHIHLKTEGWVERLFVSFTG
EECCHHHHHHHCCEECCCCCCCEEEEEEEEEEECCCCCEEEEEEEECHHHHHHHHHHHCC
QQVKAGEAMLAIYSPAFFAAQRELLSAARSAQSNLGGMTDQQLVLEAARRRLELWDVPAD
CEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCHH
AIKALEATGRPGTSLVLRSPISGTVLEKKVFQGQYTMPQNELFVVADLSTVWVQGKVFEY
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEECEEEEEECEEEEE
ELPHVQIGMAVTVALPSSPTQQYTGKVVFIDPVVDEMTRSVQVRVELPNPDGRLKPGMFS
ECCEEEEEEEEEEEECCCCCHHHCCCEEEECHHHHHCCCEEEEEEECCCCCCCCCCCHHH
QILISHAMGSGLTVPVSAVIRTGERNIAFRAVADDRFLPVQVKISPLRFEDRFQILEGLE
HHHHHHHCCCCCEEEHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHCCC
DGDMVATSANFLIDSESQLLAGGGGMAGMPGMDDGSKANENKPAARPQDVKGMSMPNRPA
CCCEEEECCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADDHSTMKHDRP
CCCCCCCCCCCC
>Mature Secondary Structure 
STKRYRKSIGPALAQLMVLCAGYFISQDASSAQQAPEPQQNMPADHSVPGNSMPPGYTE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCCCEE
LSVDPAIRQRIGVTLGRATRTPLTMTIRTVGIVRPDETKLAHIHLKTEGWVERLFVSFTG
EECCHHHHHHHCCEECCCCCCCEEEEEEEEEEECCCCCEEEEEEEECHHHHHHHHHHHCC
QQVKAGEAMLAIYSPAFFAAQRELLSAARSAQSNLGGMTDQQLVLEAARRRLELWDVPAD
CEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCHH
AIKALEATGRPGTSLVLRSPISGTVLEKKVFQGQYTMPQNELFVVADLSTVWVQGKVFEY
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEECEEEEEECEEEEE
ELPHVQIGMAVTVALPSSPTQQYTGKVVFIDPVVDEMTRSVQVRVELPNPDGRLKPGMFS
ECCEEEEEEEEEEEECCCCCHHHCCCEEEECHHHHHCCCEEEEEEECCCCCCCCCCCHHH
QILISHAMGSGLTVPVSAVIRTGERNIAFRAVADDRFLPVQVKISPLRFEDRFQILEGLE
HHHHHHHCCCCCEEEHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHCCC
DGDMVATSANFLIDSESQLLAGGGGMAGMPGMDDGSKANENKPAARPQDVKGMSMPNRPA
CCCEEEECCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADDHSTMKHDRP
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]