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The map label for this gene is shiA [H]

Identifier: 192289347

GI number: 192289347

Start: 973736

End: 975400

Strand: Reverse

Name: shiA [H]

Synonym: Rpal_0919

Alternate gene names: 192289347

Gene position: 975400-973736 (Counterclockwise)

Preceding gene: 192289349

Following gene: 192289343

Centisome position: 16.98

GC content: 63.24

Gene sequence:

>1665_bases
ATGGCTACACTTGCCGCGACGAGCAGGCGATCGGGTGGAATGACCCGCGACGAGCGCTTCGTCATTCTTGCATCTTCGCT
CGGTACCGTTTTCGAATGGTACGACTTCTATCTGTACGGTTCGCTCGCCGCGATTATCGGCGCCCAGTTCTTCAGCGCTT
ATCCGCCGGCCACCCGGGACATTTTCGCGCTGCTCGCCTTCGCCGCCGGCTTCCTGGTGCGCCCGTTCGGCGCCATCGTG
TTCGGCCGGGTCGGCGACATCGTCGGCCGTAAATACACCTTCCTGGTCACCATCCTGATCATGGGCCTGTCGACCTTTAT
CGTCGGCCTGCTGCCCAATGCGGCCACCATCGGCATTGCCGCCCCGATCATCCTGATCACGCTGCGCCTGCTGCAGGGCC
TCGCGCTCGGCGGCGAATACGGTGGCGCCGCGACCTACGTGGCCGAGCATGCTCCGCCCGGCAAGCGCGGCTACTACACC
GCATTCATTCAGACCACCGCGACCCTCGGCCTGTTCCTGTCGCTGCTGGTGATCCTTGCCACCCGCACCATCGTGGGCGA
AGTGGCGTTCGCCGATTGGGGCTGGCGCGTGCCGTTCCTGGTGTCGGTCGCCTTGCTCGGCGTCTCGGTCTGGATCCGGC
TGCGGCTCAACGAGTCGCCGGTGTTCAAGAAGATGAAGGAGGAAGGCAAGAGCTCGAAGGCGCCGCTGACCGAAGCTTTC
GCCAACTGGGGCAACGCGAAGATCGTGCTGATCGCGCTGCTCGGTGCGGTGATGGGCCAGGGCGTTGTCTGGTACACTGG
CCAGTTCTACGCGCTGTTCTTCCTGCAATCGATCCTGAAGGTCGACGGCTACACCTCGAACCTGCTGATCGCGTGGTCGC
TGCTGCTCGGCACCGGCTTCTTCATCATCTTCGGCTGGCTGTCGGACAAGATCGGCCGCAAGCCGATCATCCTCACCGGC
TGTCTGATCGCGGCACTGTCGTTCTTCCCGATCTTCCGGATGATCACGACATACGCCAACCCGGCGCTGGAAAAGGCGAT
CGAGACCGTGAAGGTGCAAGTCGTCGCCGATCCGGCGGGCTGCGGCGACCTGTTCAACCCGGTCGGCACACGCGTCTTCA
CCAAGCCGTGCGACACCGCGCGCGACTTCCTGTCGAAGTCCTCGGTGAAGTACTCCACCGTCAACGGCCCCGCCGGCTCC
GGCGTCAAGGTGATGGTGAACGAGAAGGAAGTGCCGTACACCGACGCCAAGACCTCCAACCCGCAGGTGCTGGCGGCGGT
GCAGGAAGCTGGTTACCCGAAGGCCGGCAACCCGCAGATCATCAAGATGGCGCACCCGTTCGACGTGTTCAATTCGAGCA
CCGCAGCGGTGATCGGACTGTTGTTCGTCCTGGTGCTGTTCGTCACGATGGTCTACGGCCCGATCGCGGCGCTGCTGGTC
GAACTGTTCCCGACTCGGATTCGCTATACCTCGATGTCGCTGCCGTATCACATCGGCAACGGCTGGTTCGGCGGCCTGCT
GCCGGCGACTGCGTTCGCGATCGTCGCCTCGACCGGCGATATCTATGCCGGCCTGTGGTACCCGATCGTCTTCGCGTCGA
TCACTGTGGTGATTGGCCTGATCTTCCTGCCGGAAACCAAGAACGTCGACATCAGCAAGACCTGA

Upstream 100 bases:

>100_bases
GTACACTGACGGACCGTTGCCGCGCGCCCGGCGCTGTGGCTCGGACCTCAAATAAAACAACAACATAGCCCTCGACACAA
CCCAAGGGAGGCAGACGCTG

Downstream 100 bases:

>100_bases
TGATCATCGGCCCGGCGTCTCAGCCGGACCGACCAAATAGCAACGGCCGCGGAGCAATCCGCGGCCGCTTTGTTTTAGGA
GACTGATATCCCTCGCAGCT

Product: General substrate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MATLAATSRRSGGMTRDERFVILASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIV
FGRVGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIAAPIILITLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYT
AFIQTTATLGLFLSLLVILATRTIVGEVAFADWGWRVPFLVSVALLGVSVWIRLRLNESPVFKKMKEEGKSSKAPLTEAF
ANWGNAKIVLIALLGAVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGFFIIFGWLSDKIGRKPIILTG
CLIAALSFFPIFRMITTYANPALEKAIETVKVQVVADPAGCGDLFNPVGTRVFTKPCDTARDFLSKSSVKYSTVNGPAGS
GVKVMVNEKEVPYTDAKTSNPQVLAAVQEAGYPKAGNPQIIKMAHPFDVFNSSTAAVIGLLFVLVLFVTMVYGPIAALLV
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIVFASITVVIGLIFLPETKNVDISKT

Sequences:

>Translated_554_residues
MATLAATSRRSGGMTRDERFVILASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIV
FGRVGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIAAPIILITLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYT
AFIQTTATLGLFLSLLVILATRTIVGEVAFADWGWRVPFLVSVALLGVSVWIRLRLNESPVFKKMKEEGKSSKAPLTEAF
ANWGNAKIVLIALLGAVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGFFIIFGWLSDKIGRKPIILTG
CLIAALSFFPIFRMITTYANPALEKAIETVKVQVVADPAGCGDLFNPVGTRVFTKPCDTARDFLSKSSVKYSTVNGPAGS
GVKVMVNEKEVPYTDAKTSNPQVLAAVQEAGYPKAGNPQIIKMAHPFDVFNSSTAAVIGLLFVLVLFVTMVYGPIAALLV
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIVFASITVVIGLIFLPETKNVDISKT
>Mature_553_residues
ATLAATSRRSGGMTRDERFVILASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIVF
GRVGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIAAPIILITLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYTA
FIQTTATLGLFLSLLVILATRTIVGEVAFADWGWRVPFLVSVALLGVSVWIRLRLNESPVFKKMKEEGKSSKAPLTEAFA
NWGNAKIVLIALLGAVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGFFIIFGWLSDKIGRKPIILTGC
LIAALSFFPIFRMITTYANPALEKAIETVKVQVVADPAGCGDLFNPVGTRVFTKPCDTARDFLSKSSVKYSTVNGPAGSG
VKVMVNEKEVPYTDAKTSNPQVLAAVQEAGYPKAGNPQIIKMAHPFDVFNSSTAAVIGLLFVLVLFVTMVYGPIAALLVE
LFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIVFASITVVIGLIFLPETKNVDISKT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=344, Percent_Identity=34.8837209302326, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1790550, Length=331, Percent_Identity=34.7432024169184, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1788942, Length=342, Percent_Identity=31.5789473684211, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789941, Length=332, Percent_Identity=37.0481927710843, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87082404, Length=319, Percent_Identity=25.3918495297806, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87082231, Length=218, Percent_Identity=26.1467889908257, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59682; Mature: 59551

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLAATSRRSGGMTRDERFVILASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRD
CCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IFALLAFAAGFLVRPFGAIVFGRVGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
APIILITLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYTAFIQTTATLGLFLSLLVILA
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
TRTIVGEVAFADWGWRVPFLVSVALLGVSVWIRLRLNESPVFKKMKEEGKSSKAPLTEAF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHHHHH
ANWGNAKIVLIALLGAVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGF
HCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHH
FIIFGWLSDKIGRKPIILTGCLIAALSFFPIFRMITTYANPALEKAIETVKVQVVADPAG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEECCCC
CGDLFNPVGTRVFTKPCDTARDFLSKSSVKYSTVNGPAGSGVKVMVNEKEVPYTDAKTSN
CCHHHHHHCCCEECCCCHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
PQVLAAVQEAGYPKAGNPQIIKMAHPFDVFNSSTAAVIGLLFVLVLFVTMVYGPIAALLV
CHHHHHHHHCCCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIVFASITVVIGL
HHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
IFLPETKNVDISKT
HCCCCCCCCCCCCC
>Mature Secondary Structure 
ATLAATSRRSGGMTRDERFVILASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRD
CCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IFALLAFAAGFLVRPFGAIVFGRVGDIVGRKYTFLVTILIMGLSTFIVGLLPNAATIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
APIILITLRLLQGLALGGEYGGAATYVAEHAPPGKRGYYTAFIQTTATLGLFLSLLVILA
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
TRTIVGEVAFADWGWRVPFLVSVALLGVSVWIRLRLNESPVFKKMKEEGKSSKAPLTEAF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHHHHH
ANWGNAKIVLIALLGAVMGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGF
HCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHH
FIIFGWLSDKIGRKPIILTGCLIAALSFFPIFRMITTYANPALEKAIETVKVQVVADPAG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEECCCC
CGDLFNPVGTRVFTKPCDTARDFLSKSSVKYSTVNGPAGSGVKVMVNEKEVPYTDAKTSN
CCHHHHHHCCCEECCCCHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
PQVLAAVQEAGYPKAGNPQIIKMAHPFDVFNSSTAAVIGLLFVLVLFVTMVYGPIAALLV
CHHHHHHHHCCCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFPTRIRYTSMSLPYHIGNGWFGGLLPATAFAIVASTGDIYAGLWYPIVFASITVVIGL
HHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
IFLPETKNVDISKT
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]