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The map label for this gene is dppA [H]

Identifier: 192289260

GI number: 192289260

Start: 877599

End: 879260

Strand: Reverse

Name: dppA [H]

Synonym: Rpal_0832

Alternate gene names: 192289260

Gene position: 879260-877599 (Counterclockwise)

Preceding gene: 192289263

Following gene: 192289259

Centisome position: 15.31

GC content: 63.66

Gene sequence:

>1662_bases
ATGCGTAATTTTCGTCGTAACAACCGTCGCGCTGCGCTGCTCGCTCTGTCGATGGCAAGCGTCTTCATGGTGCCGTCGCT
GGCGTCCGCCGAATCGGTGCTGCGCATCGGCATGACGGCCGCAGACATTCCGCGCACGCTCGGTCAGCCCGATCAGGGCT
TCGAAGGTAACCGCTTCACCGGGCTGACGATGTATGACGCCCTGACGATGTGGGATCTGTCGTCGGCAACCAAAGCGAGC
GTGGTTATCCCCGGCCTCGCCACCGAGTGGAAGGTCGAAGACGCTGATCATACAAAGTGGATCTTCAAACTCCGCCCCGG
CGTGAAGTTTCACGACGGCAGCGACTTCAACGCCGACGCCGTGGTGTGGAACGTCGATAAGGTGCTCAACAAAGAAGCAC
CGCAATACGATCCCAGCCAGATCGGCGTGACCGCATCGCGGATGCCGACGCTGGTCTCGGCCAAGAAGATCGATGACCTG
ACCGTCGAGCTGACGACCAAGGAGCCCGACAGCTTCCTCCCCATCAACCTCACCAACCTGTTCATGGCGAGCCCGACCAA
GTGGCAGGCGCTGTACGACAAGGCCGAGGGCGCCGACGCCAAGGCGAAGTCGACCGCCGCTTGGGCCGCGTTCGCCAAGG
ATGCGTCGGGCACCGGCCCGTGGAAGATGGCGAAGTTCACGCCGCGCGAGCGGCTGGAGCTCACCAAGAACGACGGTTAC
TGGGACAAGAACCGCGTGCCGCATGTCGACCGCATGGTTCTGCTGCCGATGCCGGAAGCCAACGCCCGGACCGCGGCGCT
GCTCTCCGGCCAGGTCGATTGGATCGAAGCACCGGCGCCGGATGCGGTGAAGGAGATCGAAGGCCGCGGCTTCAAGATCG
AGAAGAACGAACAGCCGCACGTCTGGCCGTGGCAATTCTCGCGGATCGAAGGCTCGCCGTGGAACGACATCCGCGTCCGC
CGCGCCGCCAATCTGTGTATCGATCGTGAAGGCATGCGCGACGGCCTGCTGTCGGGCCTGATGGTGCCGGCCACCGGCAC
GTTCGAGCCGGGTCACCCCTGGCGCGGCAAGCCGCAGTTCCAGATCAAGTACGATCTGCCCGCGGCGCAGAAGCTGATGA
AGGAAGCCGGCTTCGGCCCGAACAAGAAGCTCACCGTCAAGGTGCAGACCTCGGCGTCCGGCTCGGGCCAGATGCTGCCG
CTGCCGATGAACGAGTATCTGCAGCAGGCACTGGCGGAGTGTTACTTCGACGTCAAGCTCGACGTGATCGAATGGAACAC
GCTGTTCACCAATTGGCGCCGCGGCGTCAAGGATCCGTCGGCGAACGGCTCGGATGCGATCAACGTCACTTACGCGGCGA
TGGACCCGTTCTTCGCGCTGGTGCGCTTCCTGCAGTCGTCGATGGCGCCGCCGGTGTCGAACAACTGGGGTTACATCAAC
AACCCGAAGTTCGACGAGCTGGTGAAGAAGGCGCGCACCACCTTCACCGACAAGGAGCGTGACGACGTCCTCGCCGAGCT
CAACGCCGCCTCGATCGACGACGCCGCGTTCCTCTACGTCGCCCACGACGTCGGACCGCGTGCACTGAGCCCGAAGATCA
AGGGCTTCGTGCAGCCGAAGAGCTGGTTCGTCGACTTCTCCCCGGTGTCGATCGCGCCGTAA

Upstream 100 bases:

>100_bases
GTCTTCGTCGAGGCGGGCGGCGTCGGACTGCCGCTTTGGCTGGCACATGCATTGCATTGACCAGCCTGCCAATTAGTCAC
TGCAAAGGGACTCTCGTCGA

Downstream 100 bases:

>100_bases
CAGACGGCTCCCCTCTCCCCTTGTGGGAGAGGGTGGATGCGCGCCGCCAGGCGCGCAGACGGGTGAGGGGAAGACGCGGT
GACGCGTCTCTCCCGCCTCG

Product: 4-phytase

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MRNFRRNNRRAALLALSMASVFMVPSLASAESVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDALTMWDLSSATKAS
VVIPGLATEWKVEDADHTKWIFKLRPGVKFHDGSDFNADAVVWNVDKVLNKEAPQYDPSQIGVTASRMPTLVSAKKIDDL
TVELTTKEPDSFLPINLTNLFMASPTKWQALYDKAEGADAKAKSTAAWAAFAKDASGTGPWKMAKFTPRERLELTKNDGY
WDKNRVPHVDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEIEGRGFKIEKNEQPHVWPWQFSRIEGSPWNDIRVR
RAANLCIDREGMRDGLLSGLMVPATGTFEPGHPWRGKPQFQIKYDLPAAQKLMKEAGFGPNKKLTVKVQTSASGSGQMLP
LPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGVKDPSANGSDAINVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
NPKFDELVKKARTTFTDKERDDVLAELNAASIDDAAFLYVAHDVGPRALSPKIKGFVQPKSWFVDFSPVSIAP

Sequences:

>Translated_553_residues
MRNFRRNNRRAALLALSMASVFMVPSLASAESVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDALTMWDLSSATKAS
VVIPGLATEWKVEDADHTKWIFKLRPGVKFHDGSDFNADAVVWNVDKVLNKEAPQYDPSQIGVTASRMPTLVSAKKIDDL
TVELTTKEPDSFLPINLTNLFMASPTKWQALYDKAEGADAKAKSTAAWAAFAKDASGTGPWKMAKFTPRERLELTKNDGY
WDKNRVPHVDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEIEGRGFKIEKNEQPHVWPWQFSRIEGSPWNDIRVR
RAANLCIDREGMRDGLLSGLMVPATGTFEPGHPWRGKPQFQIKYDLPAAQKLMKEAGFGPNKKLTVKVQTSASGSGQMLP
LPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGVKDPSANGSDAINVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
NPKFDELVKKARTTFTDKERDDVLAELNAASIDDAAFLYVAHDVGPRALSPKIKGFVQPKSWFVDFSPVSIAP
>Mature_553_residues
MRNFRRNNRRAALLALSMASVFMVPSLASAESVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDALTMWDLSSATKAS
VVIPGLATEWKVEDADHTKWIFKLRPGVKFHDGSDFNADAVVWNVDKVLNKEAPQYDPSQIGVTASRMPTLVSAKKIDDL
TVELTTKEPDSFLPINLTNLFMASPTKWQALYDKAEGADAKAKSTAAWAAFAKDASGTGPWKMAKFTPRERLELTKNDGY
WDKNRVPHVDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEIEGRGFKIEKNEQPHVWPWQFSRIEGSPWNDIRVR
RAANLCIDREGMRDGLLSGLMVPATGTFEPGHPWRGKPQFQIKYDLPAAQKLMKEAGFGPNKKLTVKVQTSASGSGQMLP
LPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGVKDPSANGSDAINVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
NPKFDELVKKARTTFTDKERDDVLAELNAASIDDAAFLYVAHDVGPRALSPKIKGFVQPKSWFVDFSPVSIAP

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=567, Percent_Identity=24.6913580246914, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787052, Length=521, Percent_Identity=23.4165067178503, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081878, Length=483, Percent_Identity=23.8095238095238, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787762, Length=544, Percent_Identity=23.1617647058824, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787495, Length=551, Percent_Identity=22.5045372050817, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789887, Length=529, Percent_Identity=21.9281663516068, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61521; Mature: 61521

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNFRRNNRRAALLALSMASVFMVPSLASAESVLRIGMTAADIPRTLGQPDQGFEGNRFT
CCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEE
GLTMYDALTMWDLSSATKASVVIPGLATEWKVEDADHTKWIFKLRPGVKFHDGSDFNADA
EEEHHHHEEHEECCCCCCCEEEECCCCCCEEECCCCCCEEEEEECCCCEECCCCCCCCCE
VVWNVDKVLNKEAPQYDPSQIGVTASRMPTLVSAKKIDDLTVELTTKEPDSFLPINLTNL
EEEEHHHHHCCCCCCCCCHHCCEEHHHCCHHHHHHCCCCEEEEEEECCCCCEECEEHHHE
FMASPTKWQALYDKAEGADAKAKSTAAWAAFAKDASGTGPWKMAKFTPRERLELTKNDGY
EECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHEEEECCCCC
WDKNRVPHVDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEIEGRGFKIEKNEQPH
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEECCCCHHHHHHCCCCEEECCCCCCC
VWPWQFSRIEGSPWNDIRVRRAANLCIDREGMRDGLLSGLMVPATGTFEPGHPWRGKPQF
CCCCEEECCCCCCCCHHHHHHHHHHEECCCCHHHHHHCCEEECCCCCCCCCCCCCCCCCE
QIKYDLPAAQKLMKEAGFGPNKKLTVKVQTSASGSGQMLPLPMNEYLQQALAECYFDVKL
EEEECCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHEEEEEE
DVIEWNTLFTNWRRGVKDPSANGSDAINVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
EEEECHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NPKFDELVKKARTTFTDKERDDVLAELNAASIDDAAFLYVAHDVGPRALSPKIKGFVQPK
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHEEECCC
SWFVDFSPVSIAP
CEEEECCCCCCCC
>Mature Secondary Structure
MRNFRRNNRRAALLALSMASVFMVPSLASAESVLRIGMTAADIPRTLGQPDQGFEGNRFT
CCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEE
GLTMYDALTMWDLSSATKASVVIPGLATEWKVEDADHTKWIFKLRPGVKFHDGSDFNADA
EEEHHHHEEHEECCCCCCCEEEECCCCCCEEECCCCCCEEEEEECCCCEECCCCCCCCCE
VVWNVDKVLNKEAPQYDPSQIGVTASRMPTLVSAKKIDDLTVELTTKEPDSFLPINLTNL
EEEEHHHHHCCCCCCCCCHHCCEEHHHCCHHHHHHCCCCEEEEEEECCCCCEECEEHHHE
FMASPTKWQALYDKAEGADAKAKSTAAWAAFAKDASGTGPWKMAKFTPRERLELTKNDGY
EECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHEEEECCCCC
WDKNRVPHVDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEIEGRGFKIEKNEQPH
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEECCCCHHHHHHCCCCEEECCCCCCC
VWPWQFSRIEGSPWNDIRVRRAANLCIDREGMRDGLLSGLMVPATGTFEPGHPWRGKPQF
CCCCEEECCCCCCCCHHHHHHHHHHEECCCCHHHHHHCCEEECCCCCCCCCCCCCCCCCE
QIKYDLPAAQKLMKEAGFGPNKKLTVKVQTSASGSGQMLPLPMNEYLQQALAECYFDVKL
EEEECCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHEEEEEE
DVIEWNTLFTNWRRGVKDPSANGSDAINVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
EEEECHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NPKFDELVKKARTTFTDKERDDVLAELNAASIDDAAFLYVAHDVGPRALSPKIKGFVQPK
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHEEECCC
SWFVDFSPVSIAP
CEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]