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The map label for this gene is gatA [H]

Identifier: 192289255

GI number: 192289255

Start: 872858

End: 874270

Strand: Reverse

Name: gatA [H]

Synonym: Rpal_0827

Alternate gene names: 192289255

Gene position: 874270-872858 (Counterclockwise)

Preceding gene: 192289256

Following gene: 192289254

Centisome position: 15.22

GC content: 69.14

Gene sequence:

>1413_bases
ATGAGCCTCGGCAATCCCGCGCTGCTGACGCTGACCGAAGTTGCCGAGGCGATCGCGTCCAAGAAACTGTCGTCACGTGA
AGTTACCCAAGCCTGCCTCGATCGTATCGCGCGTGCGCAACCGAAGCTCAACGCCTTCATGGCGATCGAGCCTGAGAGCG
CACTCGCCGCAGCGGATGCGGCCGACACTGCACTGGCCAAGGGCGAGCGCAAAGGCCCGCTGCACGGCGTGCCGCTGGCC
CACAAGGACATGTATTACGAGACCGGCTTCGCGGTGACCTGCGGCTCCAAGATCCGCCGCGACTTCGTCGCTCCCACCAC
CTCGACCGCGCTGCAACGACTGAAGGACGCCGGCCAGGTTCGGCTCGGCACTCTGCAGATGTCCGAATTCGCCTACGGCC
CCACCGGCCACAACGCGCATTACGGCGCGGTGCGCAATCCGTGGGGCCTCGACCACATCACCGGCGGCTCGTCGTCCGGT
TCCGGCTCGGCAGTCGCCGCGCGGCTGACGTTTGCGGCGCTCGGCTCCGACACCGGCGGGTCGATCCGGATGCCGGCGCA
TTTCTGCGGCGTCACCGGGCTGAAGACCACCGTCGGGCTGGTCAGCCGCGCCGGCGCGATGCCGCTGTCGCATTCGCTCG
ACACCGTCGGGCCGCTGGCCCGCACCGCGGAGGACTGCGCGCTGCTCACCGGTCTGATGGCCGGCGCCGATCCCGCCGAT
CCGACCGCGTCGCATCGCCCGGTGCCGGACTATCTGGCCGCCACCAGACAACCGCTGCAGGGCATCAGCATCGGCATTCC
GAAATCATTCTATGTCGACGATCTCGATCCAGAGGTGGCGCGCGTGCTCGGCGACACCAAGTCGGCGCTGGCGCGCGAAG
GCGTCCGCGTCGTCGAAGTCGAACTGCCGGATCAGCGCCAGCTCAGCGCGGCCGCACAGCTCGTGCTCGCGGTCGAAGCC
GCCGGCTTCCACGGCCAGTGGATGCGCGAGCGGCCGCAGGATTACGGCCCGCAGGTGCTGATGCGGCTGCAGAACGCGCT
GGCAATTCCGGCCGTCACCTATCTCGAAGCGATGCGCTGGCGCGGCGTGGCGCTCGCCGAGTTCGTCGCGGCCGTGGCCG
GCGTCGATGCGGTGCTGGCGCCGGTGTCGCCGGTGCCAGCGCCGACGATTGCAGAAAGCGACGTCGGCAACAGCCTGAAC
GCCGAAGCTGTGATCCAGCGGCTGACCCGCTTCACCCGGCCGATCAACTATCTCGGCCTGCCGTCGCTGGCGCTGCCCTG
CGGCTTCTCGAACGCCGGCCTGCCGATCGGCCTGCAACTGATCGGCCGCCCGTTCGACGAAGCGACGCTGCTGCGGATCG
GCGCAGGCTGGCAGCGCGCCACCGACTTCCATCGGCAATGCCCGGAGCTGTAG

Upstream 100 bases:

>100_bases
CGATCGGCCCGGCGTTCGATGGCTTCACGCCGATCGCCGGTACGCTTCCATTCGATCTCGAACCATCTACGTACGTTGTA
GCGCAGAACGAGGGCGCGCG

Downstream 100 bases:

>100_bases
ATGAGCAACCTGGTCGACATCCGCGATCTCAACGTTCGCTTCACCGGCGAGCGGACCGTGTACGCCATCAACGACCTCAA
CCTCACGCTGGGCCAGGGCG

Product: Amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSLGNPALLTLTEVAEAIASKKLSSREVTQACLDRIARAQPKLNAFMAIEPESALAAADAADTALAKGERKGPLHGVPLA
HKDMYYETGFAVTCGSKIRRDFVAPTTSTALQRLKDAGQVRLGTLQMSEFAYGPTGHNAHYGAVRNPWGLDHITGGSSSG
SGSAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTVGLVSRAGAMPLSHSLDTVGPLARTAEDCALLTGLMAGADPAD
PTASHRPVPDYLAATRQPLQGISIGIPKSFYVDDLDPEVARVLGDTKSALAREGVRVVEVELPDQRQLSAAAQLVLAVEA
AGFHGQWMRERPQDYGPQVLMRLQNALAIPAVTYLEAMRWRGVALAEFVAAVAGVDAVLAPVSPVPAPTIAESDVGNSLN
AEAVIQRLTRFTRPINYLGLPSLALPCGFSNAGLPIGLQLIGRPFDEATLLRIGAGWQRATDFHRQCPEL

Sequences:

>Translated_470_residues
MSLGNPALLTLTEVAEAIASKKLSSREVTQACLDRIARAQPKLNAFMAIEPESALAAADAADTALAKGERKGPLHGVPLA
HKDMYYETGFAVTCGSKIRRDFVAPTTSTALQRLKDAGQVRLGTLQMSEFAYGPTGHNAHYGAVRNPWGLDHITGGSSSG
SGSAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTVGLVSRAGAMPLSHSLDTVGPLARTAEDCALLTGLMAGADPAD
PTASHRPVPDYLAATRQPLQGISIGIPKSFYVDDLDPEVARVLGDTKSALAREGVRVVEVELPDQRQLSAAAQLVLAVEA
AGFHGQWMRERPQDYGPQVLMRLQNALAIPAVTYLEAMRWRGVALAEFVAAVAGVDAVLAPVSPVPAPTIAESDVGNSLN
AEAVIQRLTRFTRPINYLGLPSLALPCGFSNAGLPIGLQLIGRPFDEATLLRIGAGWQRATDFHRQCPEL
>Mature_469_residues
SLGNPALLTLTEVAEAIASKKLSSREVTQACLDRIARAQPKLNAFMAIEPESALAAADAADTALAKGERKGPLHGVPLAH
KDMYYETGFAVTCGSKIRRDFVAPTTSTALQRLKDAGQVRLGTLQMSEFAYGPTGHNAHYGAVRNPWGLDHITGGSSSGS
GSAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTVGLVSRAGAMPLSHSLDTVGPLARTAEDCALLTGLMAGADPADP
TASHRPVPDYLAATRQPLQGISIGIPKSFYVDDLDPEVARVLGDTKSALAREGVRVVEVELPDQRQLSAAAQLVLAVEAA
GFHGQWMRERPQDYGPQVLMRLQNALAIPAVTYLEAMRWRGVALAEFVAAVAGVDAVLAPVSPVPAPTIAESDVGNSLNA
EAVIQRLTRFTRPINYLGLPSLALPCGFSNAGLPIGLQLIGRPFDEATLLRIGAGWQRATDFHRQCPEL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=31.610337972167, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=27.7665995975855, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI166795287, Length=244, Percent_Identity=29.0983606557377, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=29.2383292383292, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537465, Length=494, Percent_Identity=26.5182186234818, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=364, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=27.6872964169381, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=307, Percent_Identity=27.6872964169381, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=359, Percent_Identity=22.5626740947075, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=310, Percent_Identity=23.8709677419355, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=463, Percent_Identity=26.7818574514039, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319685, Length=399, Percent_Identity=28.0701754385965, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=227, Percent_Identity=29.0748898678414, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=33.265306122449, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24652985, Length=520, Percent_Identity=26.1538461538462, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922090, Length=520, Percent_Identity=26.1538461538462, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24652981, Length=520, Percent_Identity=26.1538461538462, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24652983, Length=520, Percent_Identity=26.1538461538462, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=27.4549098196393, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=26.6, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356731, Length=482, Percent_Identity=25.7261410788382, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45550774, Length=484, Percent_Identity=24.3801652892562, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648435, Length=479, Percent_Identity=25.0521920668058, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648437, Length=479, Percent_Identity=25.0521920668058, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648441, Length=416, Percent_Identity=24.5192307692308, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648439, Length=416, Percent_Identity=24.5192307692308, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49448; Mature: 49317

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGNPALLTLTEVAEAIASKKLSSREVTQACLDRIARAQPKLNAFMAIEPESALAAADA
CCCCCCHHEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
ADTALAKGERKGPLHGVPLAHKDMYYETGFAVTCGSKIRRDFVAPTTSTALQRLKDAGQV
HHHHHHCCCCCCCCCCCCCCCCCCEEECCEEEECCHHHHHHHCCCCHHHHHHHHHHCCCE
RLGTLQMSEFAYGPTGHNAHYGAVRNPWGLDHITGGSSSGSGSAVAARLTFAALGSDTGG
EEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
SIRMPAHFCGVTGLKTTVGLVSRAGAMPLSHSLDTVGPLARTAEDCALLTGLMAGADPAD
CEECCHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTASHRPVPDYLAATRQPLQGISIGIPKSFYVDDLDPEVARVLGDTKSALAREGVRVVEV
CCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCEEEEE
ELPDQRQLSAAAQLVLAVEAAGFHGQWMRERPQDYGPQVLMRLQNALAIPAVTYLEAMRW
ECCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGVALAEFVAAVAGVDAVLAPVSPVPAPTIAESDVGNSLNAEAVIQRLTRFTRPINYLGL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
PSLALPCGFSNAGLPIGLQLIGRPFDEATLLRIGAGWQRATDFHRQCPEL
CCHHCCCCCCCCCCCEEHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLGNPALLTLTEVAEAIASKKLSSREVTQACLDRIARAQPKLNAFMAIEPESALAAADA
CCCCCHHEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
ADTALAKGERKGPLHGVPLAHKDMYYETGFAVTCGSKIRRDFVAPTTSTALQRLKDAGQV
HHHHHHCCCCCCCCCCCCCCCCCCEEECCEEEECCHHHHHHHCCCCHHHHHHHHHHCCCE
RLGTLQMSEFAYGPTGHNAHYGAVRNPWGLDHITGGSSSGSGSAVAARLTFAALGSDTGG
EEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
SIRMPAHFCGVTGLKTTVGLVSRAGAMPLSHSLDTVGPLARTAEDCALLTGLMAGADPAD
CEECCHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTASHRPVPDYLAATRQPLQGISIGIPKSFYVDDLDPEVARVLGDTKSALAREGVRVVEV
CCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCEEEEE
ELPDQRQLSAAAQLVLAVEAAGFHGQWMRERPQDYGPQVLMRLQNALAIPAVTYLEAMRW
ECCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGVALAEFVAAVAGVDAVLAPVSPVPAPTIAESDVGNSLNAEAVIQRLTRFTRPINYLGL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
PSLALPCGFSNAGLPIGLQLIGRPFDEATLLRIGAGWQRATDFHRQCPEL
CCHHCCCCCCCCCCCEEHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA