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The map label for this gene is cysH [H]

Identifier: 192289250

GI number: 192289250

Start: 866534

End: 867274

Strand: Reverse

Name: cysH [H]

Synonym: Rpal_0822

Alternate gene names: 192289250

Gene position: 867274-866534 (Counterclockwise)

Preceding gene: 192289253

Following gene: 192289249

Centisome position: 15.1

GC content: 63.7

Gene sequence:

>741_bases
ATGACCGAGCTGGCGCAATCTCTCCCCCCCTCCACTTCCGTTTCCACGGGTGTGGAGACGCGTGCGCAGGCACTCGACGC
AGCGCTTCGAACCGCATCGCCGGCCGAAGTCATTGCCGCGGCGCTGCGCGAGGTCGGTCGCGACAAGCTGGCGCTGGTGT
CCTCATTCGGCACCGAGTCGGCGGCGCTGTTGAAAGTGATGGCGGATGTCGATCCGGCAATTCCGGTAGTGTTTCTCGAT
ACCGGTTGGCTGTTCGAAGAAACCCTCGCCTATCGCGATACGCTTACCAACGTGCTCGGCCTTCGTGACGTACGTTCGAT
CAAGCCGCTCGACGCCGATCTCGATCGCGACGATGCGGCGCGCGAGTTGTGGTTTTCCGATCCGGACAAGTGCTGCCTGA
TCCGCAAGGTCGAGCCGCTGAAGCGGGCACTGGCGCCGTTCGACGGCTGGATCAACGGTCGCAAGCGCTTCCAGGGCGGT
GCGCGCGCCGACATTCCGGTGGTCGAAGCCGACGGCAAGCGGCTGAAGTTCAATCCGTTTGCGAATATCACGCCGACCGA
CATCGCGGCGATCTACGCCACCGCAAAGCTGCCGCAGCATCCGTTACTCGCGTCGGGTTTTCTCTCCGTCGGCTGCATGC
CGTGTACCAGCCGCGCCGAAACCGGTGAGGATGCACGCGCCGGCCGCTGGCGTGGACGCGGCAAGACGGAATGCGGCATT
CACACGATCAAGACTTCATAG

Upstream 100 bases:

>100_bases
CTAGTCAATCCTCCTTCGTGGTCGGGCGAGATGCTGAGCACAATGGCGGAGTGATCGACCGCGATGATCAGGACGTTGTG
ACCTCAGGACCTAGTGACCG

Downstream 100 bases:

>100_bases
GACATTGCTGTCGCAACTAAGCGAACATTGAAATGCCGTTTCGTTGACTTGGCCTGCTTTGTTCGTGACCTTCGCCCCTC
CGCGCCGACGACACCGACGT

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTELAQSLPPSTSVSTGVETRAQALDAALRTASPAEVIAAALREVGRDKLALVSSFGTESAALLKVMADVDPAIPVVFLD
TGWLFEETLAYRDTLTNVLGLRDVRSIKPLDADLDRDDAARELWFSDPDKCCLIRKVEPLKRALAPFDGWINGRKRFQGG
ARADIPVVEADGKRLKFNPFANITPTDIAAIYATAKLPQHPLLASGFLSVGCMPCTSRAETGEDARAGRWRGRGKTECGI
HTIKTS

Sequences:

>Translated_246_residues
MTELAQSLPPSTSVSTGVETRAQALDAALRTASPAEVIAAALREVGRDKLALVSSFGTESAALLKVMADVDPAIPVVFLD
TGWLFEETLAYRDTLTNVLGLRDVRSIKPLDADLDRDDAARELWFSDPDKCCLIRKVEPLKRALAPFDGWINGRKRFQGG
ARADIPVVEADGKRLKFNPFANITPTDIAAIYATAKLPQHPLLASGFLSVGCMPCTSRAETGEDARAGRWRGRGKTECGI
HTIKTS
>Mature_245_residues
TELAQSLPPSTSVSTGVETRAQALDAALRTASPAEVIAAALREVGRDKLALVSSFGTESAALLKVMADVDPAIPVVFLDT
GWLFEETLAYRDTLTNVLGLRDVRSIKPLDADLDRDDAARELWFSDPDKCCLIRKVEPLKRALAPFDGWINGRKRFQGGA
RADIPVVEADGKRLKFNPFANITPTDIAAIYATAKLPQHPLLASGFLSVGCMPCTSRAETGEDARAGRWRGRGKTECGIH
TIKTS

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=198, Percent_Identity=32.8282828282828, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325425, Length=229, Percent_Identity=28.82096069869, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 26579; Mature: 26447

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELAQSLPPSTSVSTGVETRAQALDAALRTASPAEVIAAALREVGRDKLALVSSFGTES
CCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCH
AALLKVMADVDPAIPVVFLDTGWLFEETLAYRDTLTNVLGLRDVRSIKPLDADLDRDDAA
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RELWFSDPDKCCLIRKVEPLKRALAPFDGWINGRKRFQGGARADIPVVEADGKRLKFNPF
HHHCCCCCCCEEEEHHHHHHHHHHCCHHHHHCCHHHHCCCCCCCCEEEECCCCEEEECCC
ANITPTDIAAIYATAKLPQHPLLASGFLSVGCMPCTSRAETGEDARAGRWRGRGKTECGI
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
HTIKTS
EEEECC
>Mature Secondary Structure 
TELAQSLPPSTSVSTGVETRAQALDAALRTASPAEVIAAALREVGRDKLALVSSFGTES
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCH
AALLKVMADVDPAIPVVFLDTGWLFEETLAYRDTLTNVLGLRDVRSIKPLDADLDRDDAA
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RELWFSDPDKCCLIRKVEPLKRALAPFDGWINGRKRFQGGARADIPVVEADGKRLKFNPF
HHHCCCCCCCEEEEHHHHHHHHHHCCHHHHHCCHHHHCCCCCCCCEEEECCCCEEEECCC
ANITPTDIAAIYATAKLPQHPLLASGFLSVGCMPCTSRAETGEDARAGRWRGRGKTECGI
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
HTIKTS
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]