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The map label for this gene is palH [H]

Identifier: 192288813

GI number: 192288813

Start: 405710

End: 406990

Strand: Reverse

Name: palH [H]

Synonym: Rpal_0382

Alternate gene names: 192288813

Gene position: 406990-405710 (Counterclockwise)

Preceding gene: 192288820

Following gene: 192288810

Centisome position: 7.09

GC content: 64.01

Gene sequence:

>1281_bases
ATGACCAACACCACCAAGATCGTGTTTCTCGGCGCCTCCAGTGCGTCGTTCGGTCTCAGCATGTTTCGTGACCTGTTCGC
TTCGCCGGTGCTGGCCGGTTCGACGCTGACGCTGGTCGGACGCAATGCGGAAACGCTCGGCAGAATGACCGAGCTGGCGA
AGCTGATGAACGCAAGGTCCGGTGCCGGACTGATCATCGAGCAGACCACCGACCGCCACGCCGCGCTGGACGGCGCCGGC
TTCGTCATCAACGCCACCGCGATCGATCGCAACCGGCTGTGGAAGCAGGACTTCGAGGTGCCGAAGAAGCACGGCATCCG
TCACACCCTCGGCGAGAACGGCGGCCCGGGCGGATTGTTCTTCACGCTGCGCACGCTCCCCTTGGTGTTCGACTTCATCC
GCGACATCGAGGAGCTGTGCCCGAACGCGCTGTTTCTCAACTACTCCAATCCCGAAAGCCGGATCATTCTGGCGCTCGGC
CGCTACACCAAGGTGCGCCATATCGGACTGTGTCACGGCATCTTCATGGGCCGCGACGCGGTCGCCTACATCATGCAGAT
GCCGCGCGAAGAGATCGAAGTGTGGGGCGCCGGGCTCAATCACTTCCAGTGCTTGACCGAGATCCGCCACCGTGACACCG
GCGAGGATCTGTATCCGCGGTTTCGCGCCGCCGAGCAGAGCTTTGATCCGGATGCGTGGCGGTTCACGCGACGGCTGTAT
CGCGCGTTCGGCTATTGGCTGACCTGCAGCGATGATCATCTCGGCGAGTATCTGCCGTATGGCTGGGAAGCCGGCGAGAA
GGGCTACGATTTCGACCAGGACGAACGCTGGCGCGGCGAATTCCTCACCCAGCTGAATGGCGTGCTCGGCGGAACCATGC
CGATCCCGCGGTGGTGGACCGAACCGTCGGGCGAGCGCGGCGCCGCCGTGATCGCCGCGATGCTGCACAACCAGAAGCGT
TTCATCGAATCCGGCATCGTGCTCAATCGCGGCGTGATCCCCAACCTGCCGGCGGAGCTCGCGGTCGAAGTCCCGGTGAC
TGTAGATGCGGCCGGCGTGCATCCGGTGTCGCTCGGCCCGTTGCCCGACCCGATCGCCAAGCTGATGCTGATGCAGGCCA
GCGTGCAGCAGCTCGCGGTCGAGGCGGCCGTCCACGCCTCGAAGGAACTGGCCCTGCAGGCGCTGCTGATCGATCCGGTG
GTCAACTCGGCGGTCGCGGCCGAAAAGATCCTGGACGAATTGTGGGAGATCAACCGGCCGTATATTCGGAAGTGTGTGTA
G

Upstream 100 bases:

>100_bases
TTCACTTCATATCCGTTCGCAGCGGATAGCAGGCGAAAGCCCGTGCGCGGATCGCCCCGTCTCACGCGGCTAAACGGCCT
GCAGGCGGAGGCGATCTGAG

Downstream 100 bases:

>100_bases
CGTTACTTCGCTGCCCGTCATTGTGAGCGAAGCGAAGAAATCCAGTGGTCATTCCGGGGCGCTTGCGTAGCAAGCGAACC
CGGAATCCCGACGTAGTGGA

Product: glycoside hydrolase family 4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTNTTKIVFLGASSASFGLSMFRDLFASPVLAGSTLTLVGRNAETLGRMTELAKLMNARSGAGLIIEQTTDRHAALDGAG
FVINATAIDRNRLWKQDFEVPKKHGIRHTLGENGGPGGLFFTLRTLPLVFDFIRDIEELCPNALFLNYSNPESRIILALG
RYTKVRHIGLCHGIFMGRDAVAYIMQMPREEIEVWGAGLNHFQCLTEIRHRDTGEDLYPRFRAAEQSFDPDAWRFTRRLY
RAFGYWLTCSDDHLGEYLPYGWEAGEKGYDFDQDERWRGEFLTQLNGVLGGTMPIPRWWTEPSGERGAAVIAAMLHNQKR
FIESGIVLNRGVIPNLPAELAVEVPVTVDAAGVHPVSLGPLPDPIAKLMLMQASVQQLAVEAAVHASKELALQALLIDPV
VNSAVAAEKILDELWEINRPYIRKCV

Sequences:

>Translated_426_residues
MTNTTKIVFLGASSASFGLSMFRDLFASPVLAGSTLTLVGRNAETLGRMTELAKLMNARSGAGLIIEQTTDRHAALDGAG
FVINATAIDRNRLWKQDFEVPKKHGIRHTLGENGGPGGLFFTLRTLPLVFDFIRDIEELCPNALFLNYSNPESRIILALG
RYTKVRHIGLCHGIFMGRDAVAYIMQMPREEIEVWGAGLNHFQCLTEIRHRDTGEDLYPRFRAAEQSFDPDAWRFTRRLY
RAFGYWLTCSDDHLGEYLPYGWEAGEKGYDFDQDERWRGEFLTQLNGVLGGTMPIPRWWTEPSGERGAAVIAAMLHNQKR
FIESGIVLNRGVIPNLPAELAVEVPVTVDAAGVHPVSLGPLPDPIAKLMLMQASVQQLAVEAAVHASKELALQALLIDPV
VNSAVAAEKILDELWEINRPYIRKCV
>Mature_425_residues
TNTTKIVFLGASSASFGLSMFRDLFASPVLAGSTLTLVGRNAETLGRMTELAKLMNARSGAGLIIEQTTDRHAALDGAGF
VINATAIDRNRLWKQDFEVPKKHGIRHTLGENGGPGGLFFTLRTLPLVFDFIRDIEELCPNALFLNYSNPESRIILALGR
YTKVRHIGLCHGIFMGRDAVAYIMQMPREEIEVWGAGLNHFQCLTEIRHRDTGEDLYPRFRAAEQSFDPDAWRFTRRLYR
AFGYWLTCSDDHLGEYLPYGWEAGEKGYDFDQDERWRGEFLTQLNGVLGGTMPIPRWWTEPSGERGAAVIAAMLHNQKRF
IESGIVLNRGVIPNLPAELAVEVPVTVDAAGVHPVSLGPLPDPIAKLMLMQASVQQLAVEAAVHASKELALQALLIDPVV
NSAVAAEKILDELWEINRPYIRKCV

Specific function: Alpha-glucosidase with broad specificity. Hydrolyzes maltose, palatinose, maltulose, trehalose, trehalulose, turanose, leucrose, sucrose and maltitol. Is not active against alpha- galactosides, e.g. melibiose, and alpha-mannosides. Shows an obligate requi

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=432, Percent_Identity=29.1666666666667, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788029, Length=392, Percent_Identity=27.2959183673469, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 47417; Mature: 47286

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTTKIVFLGASSASFGLSMFRDLFASPVLAGSTLTLVGRNAETLGRMTELAKLMNARS
CCCCEEEEEEECCCCHHHHHHHHHHHHCCHHCCCEEEEECCCHHHHHHHHHHHHHHCCCC
GAGLIIEQTTDRHAALDGAGFVINATAIDRNRLWKQDFEVPKKHGIRHTLGENGGPGGLF
CCCEEEEECCCCHHHCCCCCEEEEEEEECHHHHHHHHCCCCHHCCCHHHCCCCCCCCHHH
FTLRTLPLVFDFIRDIEELCPNALFLNYSNPESRIILALGRYTKVRHIGLCHGIFMGRDA
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHH
VAYIMQMPREEIEVWGAGLNHFQCLTEIRHRDTGEDLYPRFRAAEQSFDPDAWRFTRRLY
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
RAFGYWLTCSDDHLGEYLPYGWEAGEKGYDFDQDERWRGEFLTQLNGVLGGTMPIPRWWT
HHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
EPSGERGAAVIAAMLHNQKRFIESGIVLNRGVIPNLPAELAVEVPVTVDAAGVHPVSLGP
CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHEEECCEEEECCCCCCCCCCC
LPDPIAKLMLMQASVQQLAVEAAVHASKELALQALLIDPVVNSAVAAEKILDELWEINRP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YIRKCV
HHHHCC
>Mature Secondary Structure 
TNTTKIVFLGASSASFGLSMFRDLFASPVLAGSTLTLVGRNAETLGRMTELAKLMNARS
CCCEEEEEEECCCCHHHHHHHHHHHHCCHHCCCEEEEECCCHHHHHHHHHHHHHHCCCC
GAGLIIEQTTDRHAALDGAGFVINATAIDRNRLWKQDFEVPKKHGIRHTLGENGGPGGLF
CCCEEEEECCCCHHHCCCCCEEEEEEEECHHHHHHHHCCCCHHCCCHHHCCCCCCCCHHH
FTLRTLPLVFDFIRDIEELCPNALFLNYSNPESRIILALGRYTKVRHIGLCHGIFMGRDA
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHH
VAYIMQMPREEIEVWGAGLNHFQCLTEIRHRDTGEDLYPRFRAAEQSFDPDAWRFTRRLY
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
RAFGYWLTCSDDHLGEYLPYGWEAGEKGYDFDQDERWRGEFLTQLNGVLGGTMPIPRWWT
HHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
EPSGERGAAVIAAMLHNQKRFIESGIVLNRGVIPNLPAELAVEVPVTVDAAGVHPVSLGP
CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHEEECCEEEECCCCCCCCCCC
LPDPIAKLMLMQASVQQLAVEAAVHASKELALQALLIDPVVNSAVAAEKILDELWEINRP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YIRKCV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11274100 [H]