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The map label for this gene is rph [H]

Identifier: 192288763

GI number: 192288763

Start: 350471

End: 351184

Strand: Reverse

Name: rph [H]

Synonym: Rpal_0332

Alternate gene names: 192288763

Gene position: 351184-350471 (Counterclockwise)

Preceding gene: 192288766

Following gene: 192288762

Centisome position: 6.11

GC content: 68.07

Gene sequence:

>714_bases
ATGCGGCCGAGCCGCCGTGCGCCCGACGAATTGCGCGCCGTGTCGCTGGAACGCGGCGTGGTCAAATATGCCGAGGGCTC
CTGCCTGGTGAAGTTCGGCGACACCCATGTGCTGGTGACCGCCACCCTGGAAGAACGGCTGCCGCCGTGGCTGAAGGGCC
AGGGCCGCGGCTGGGTGACGGCCGAATACGGCATGCTGCCGCGCGCCACCCTTGAGCGCACCCGCCGCGAAGCCTCCGCC
GGCAAGCAGAACGGCCGCACCGTCGAGATTCAGCGCCTCATCGGCCGCTCGCTGCGCACCATCGTCGATCTCGAAGCGCT
CGGCGAGCGCCAGATCACGGTGGACTGCGACGTGCTGCAGGCCGATGGCGGCACCCGGACGGCCTCGATCACCGGCGCCT
GGGTCGCGCTTGCGGACTGCCTGAACTGGATGAAGGCCCGCAACATGATCAAGGGCCAGGTGCTTCGCGACAACGTCGCG
GCGATCTCCTGCGGGATCTACAACGGTACGCCGGTGCTCGATCTCGACTACGCCGAGGATTCCGAGGCTGAGACCGACGC
CAACTTCGTGATGACCGGCGACGGCCGTCTGGTCGAAGTGCAGGGCACCGCGGAACGCACTCCGTTCTCGCAGGACGAGT
TCCTCCAGCTGATGGCGCTGGCGCAGAAGGGCGTCGCCCGGCTGGTGGACCTGCAGAAAATGGCGGTTGGGTGA

Upstream 100 bases:

>100_bases
GAGCGCAGGGTTGACGGCCGCAACCCTTGCCGCTGCGGCTGCGGCCCCCTAAAAGCACCGCAACGTTGATTTTCCCCCCC
GCAATAGATGGAGGTCCCCC

Downstream 100 bases:

>100_bases
GTCATTCCTGCCACTTCTTTGCAACGCGAGCTGAGCTAGGCTGACATCGCCATGCACCGTCGAATCACCGGCAAGCTCGT
GATCGCCACCCACAATCCCG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVTAEYGMLPRATLERTRREASA
GKQNGRTVEIQRLIGRSLRTIVDLEALGERQITVDCDVLQADGGTRTASITGAWVALADCLNWMKARNMIKGQVLRDNVA
AISCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRLVEVQGTAERTPFSQDEFLQLMALAQKGVARLVDLQKMAVG

Sequences:

>Translated_237_residues
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVTAEYGMLPRATLERTRREASA
GKQNGRTVEIQRLIGRSLRTIVDLEALGERQITVDCDVLQADGGTRTASITGAWVALADCLNWMKARNMIKGQVLRDNVA
AISCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRLVEVQGTAERTPFSQDEFLQLMALAQKGVARLVDLQKMAVG
>Mature_237_residues
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVTAEYGMLPRATLERTRREASA
GKQNGRTVEIQRLIGRSLRTIVDLEALGERQITVDCDVLQADGGTRTASITGAWVALADCLNWMKARNMIKGQVLRDNVA
AISCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRLVEVQGTAERTPFSQDEFLQLMALAQKGVARLVDLQKMAVG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=62.085308056872, Blast_Score=263, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71981632, Length=177, Percent_Identity=24.8587570621469, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25980; Mature: 25980

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVT
CCCCCCCCHHHHHHHHHHCEEEECCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCEEEE
AEYGMLPRATLERTRREASAGKQNGRTVEIQRLIGRSLRTIVDLEALGERQITVDCDVLQ
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAWVALADCLNWMKARNMIKGQVLRDNVAAISCGIYNGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCEEEEECCCC
SEAETDANFVMTGDGRLVEVQGTAERTPFSQDEFLQLMALAQKGVARLVDLQKMAVG
CCCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVT
CCCCCCCCHHHHHHHHHHCEEEECCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCEEEE
AEYGMLPRATLERTRREASAGKQNGRTVEIQRLIGRSLRTIVDLEALGERQITVDCDVLQ
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAWVALADCLNWMKARNMIKGQVLRDNVAAISCGIYNGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEEECCCCCCEEEEECCCC
SEAETDANFVMTGDGRLVEVQGTAERTPFSQDEFLQLMALAQKGVARLVDLQKMAVG
CCCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]