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The map label for this gene is leuC [H]

Identifier: 192288671

GI number: 192288671

Start: 257510

End: 258919

Strand: Reverse

Name: leuC [H]

Synonym: Rpal_0240

Alternate gene names: 192288671

Gene position: 258919-257510 (Counterclockwise)

Preceding gene: 192288672

Following gene: 192288666

Centisome position: 4.51

GC content: 66.38

Gene sequence:

>1410_bases
ATGTCCGCTAAGCCCACCACGCTGTACGACAAGATCTGGAACGACCATCTGGTCCACGAGGCCGAAGACGGCACCTGCCT
GCTGTATATCGACCGCCATCTGGTGCACGAAGTGACTTCGCCGCAGGCGTTTGAAGGTCTGCGCACCGCGGGCCGCAAGG
TGCACGCGCCGGAGAAGACGCTGGCGGTGGTCGATCACAACGTGCCGACCACGGATCGCTCCAAGCCCAATCCCGATCCG
GAAAGCGCCGAGCAGATTGCCGCGCTGGCCGAGAACGCCCGTGACTTCGGCATCACCTATTACAACGAGTTCGACAAGCG
TCAGGGCGTGGTCCACGTCATCGGCCCGGAGCAGGGCTTCACGCTGCCCGGCACCACCATCGTCTGCGGTGACAGCCACA
CCTCGACCCATGGGGCGTTCGGCGCGCTGGCGCACGGCATCGGCACCTCTGAGGTCGAGCACGTGCTGGCGACCCAGACA
CTGATCCAGAAGAAGGCCAAGAATATGCGGGTGACGGTCGACGGCCAATTGCCGGACGGCGTCACCGCCAAGGACGTGAT
CCTGGCGATCATCGGCGAGATCGGCACCGCGGGCGGCACCGGCTATGTGCTGGAATACGCCGGCGACGCGATCCGCTCGC
TGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATCGAAGGCGGCGCCCGCGCCGGCCTGATCGCGCCCGACGCC
AAGGCTTATGAGTTCCTGAAGGGCCGCCCGCTGGCCCCGAAGGGCGAGGCCTGGGACGCAGCGCTGCGCTATTGGGAGAC
GCTGCGCTCCGACGACGGCGCGCATTTCGATCACGAGCTCAAGCTCGACGCCGCTGCGCTGCCGCCGATCGTCACCTGGG
GCACTTCGCCTGAGGACGTGATCTCGGTCACCGGCCGCGTGCCGAACCCGGCCGACATCGCCGATGAGGCCAAGCGGCTC
TCCAAGGAGCGCGCGCTGGCCTATATGGGTCTGACCCCGGGCACCAAGATCACCGACATCAAGATCGACCGGATGTTCAT
CGGCTCCTGCACCAACGGCCGCATCGAAGATCTGCGCGCCGCCGCCAAGGTCGCCGAGGGCAAGACCGTCAACGCCAACG
TCAACGCCATCATCGTGCCGGGCTCCGGTCTGGTGAAGGAGCAGGCCGAGGCCGAGGGTCTGGACAAGATCTTCATCAAG
GCCGGCTTCGAATGGCGCGAGCCGGGCTGCTCGATGTGTCTCGCCATGAACCCCGACAAGCTGGCGCCGGAAGAGCGCTG
CGCCTCGACCTCGAACCGCAACTTCGAGGGCCGTCAGGGCTTCAAGGGCCGCACCCATCTGGTGTCGCCGGCAATGGCCG
CCGCCGCCGCGATCGCCGGCCACTTCGTCGACATCCGCGACTGGCACTAA

Upstream 100 bases:

>100_bases
ACCACGGCCGCGCTGATTAGCTGATCGGCCGACGGCGCGTTCGCGCCGCGCGATCCTGCTCACTTCGACAATTCAAAGAT
CCTTCCGAAGGACCGACACC

Downstream 100 bases:

>100_bases
GCACCGGTCGAACTAACAACGAAACAGGCGCCCCTCGGGGCGCCTGTTTTGCTTTTTCGGGATCGGTCAGTACACCCGCC
AGTGGCGGTGATGATGGTGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKTLAVVDHNVPTTDRSKPNPDP
ESAEQIAALAENARDFGITYYNEFDKRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMRVTVDGQLPDGVTAKDVILAIIGEIGTAGGTGYVLEYAGDAIRSLSMEGRMTVCNMSIEGGARAGLIAPDA
KAYEFLKGRPLAPKGEAWDAALRYWETLRSDDGAHFDHELKLDAAALPPIVTWGTSPEDVISVTGRVPNPADIADEAKRL
SKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRAAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFIK
AGFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWH

Sequences:

>Translated_469_residues
MSAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKTLAVVDHNVPTTDRSKPNPDP
ESAEQIAALAENARDFGITYYNEFDKRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMRVTVDGQLPDGVTAKDVILAIIGEIGTAGGTGYVLEYAGDAIRSLSMEGRMTVCNMSIEGGARAGLIAPDA
KAYEFLKGRPLAPKGEAWDAALRYWETLRSDDGAHFDHELKLDAAALPPIVTWGTSPEDVISVTGRVPNPADIADEAKRL
SKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRAAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFIK
AGFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWH
>Mature_468_residues
SAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKTLAVVDHNVPTTDRSKPNPDPE
SAEQIAALAENARDFGITYYNEFDKRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMRVTVDGQLPDGVTAKDVILAIIGEIGTAGGTGYVLEYAGDAIRSLSMEGRMTVCNMSIEGGARAGLIAPDAK
AYEFLKGRPLAPKGEAWDAALRYWETLRSDDGAHFDHELKLDAAALPPIVTWGTSPEDVISVTGRVPNPADIADEAKRLS
KERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRAAAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFIKA
GFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=474, Percent_Identity=26.1603375527426, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786259, Length=461, Percent_Identity=63.5574837310195, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.5927977839335, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081781, Length=340, Percent_Identity=25.5882352941176, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=476, Percent_Identity=27.3109243697479, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=387, Percent_Identity=28.6821705426357, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=29.3159609120521, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=382, Percent_Identity=28.2722513089005, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=63.5789473684211, Blast_Score=624, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6320440, Length=427, Percent_Identity=25.0585480093677, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=27.9778393351801, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=481, Percent_Identity=26.6112266112266, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281365315, Length=476, Percent_Identity=25.2100840336134, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17864292, Length=476, Percent_Identity=25.2100840336134, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=25.9162303664921, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571643, Length=475, Percent_Identity=25.0526315789474, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645686, Length=378, Percent_Identity=26.4550264550265, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50465; Mature: 50333

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKT
CCCCCCHHHHHHCCCCCEEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCE
LAVVDHNVPTTDRSKPNPDPESAEQIAALAENARDFGITYYNEFDKRQGVVHVIGPEQGF
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHCCEEEECHHHHHCCEEEEECCCCCC
TLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVTVDGQLPDG
CCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
VTAKDVILAIIGEIGTAGGTGYVLEYAGDAIRSLSMEGRMTVCNMSIEGGARAGLIAPDA
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCH
KAYEFLKGRPLAPKGEAWDAALRYWETLRSDDGAHFDHELKLDAAALPPIVTWGTSPEDV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECHHCCCCEEECCCCHHHH
ISVTGRVPNPADIADEAKRLSKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRA
HEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHH
AAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFIKAGFEWREPGCSMCLAMNPDK
HHHHHCCCEEECCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWH
CCCHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
SAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKT
CCCCCHHHHHHCCCCCEEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCE
LAVVDHNVPTTDRSKPNPDPESAEQIAALAENARDFGITYYNEFDKRQGVVHVIGPEQGF
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHCCEEEECHHHHHCCEEEEECCCCCC
TLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVTVDGQLPDG
CCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
VTAKDVILAIIGEIGTAGGTGYVLEYAGDAIRSLSMEGRMTVCNMSIEGGARAGLIAPDA
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCH
KAYEFLKGRPLAPKGEAWDAALRYWETLRSDDGAHFDHELKLDAAALPPIVTWGTSPEDV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECHHCCCCEEECCCCHHHH
ISVTGRVPNPADIADEAKRLSKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRA
HEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHH
AAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFIKAGFEWREPGCSMCLAMNPDK
HHHHHCCCEEECCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWH
CCCHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA