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The map label for this gene is leuD [H]

Identifier: 192288665

GI number: 192288665

Start: 254159

End: 254764

Strand: Reverse

Name: leuD [H]

Synonym: Rpal_0234

Alternate gene names: 192288665

Gene position: 254764-254159 (Counterclockwise)

Preceding gene: 192288666

Following gene: 192288664

Centisome position: 4.44

GC content: 60.89

Gene sequence:

>606_bases
ATGGACAAGTTCACCACGCTGGAAGGCGTCGCTGCGCCGCTGAAGATCATCAATGTCGACACCGACATGATCATTCCCAA
GCAGTATCTGAAGACCATCAAGCGCACCGGTCTCGGCAAGGGCCTGTTCTCGGAGCAGCGCTACAAGGACGACGGCAGCG
AGAACCCGGATTTCATCCTCAACAAGCCGGCCTATCGCGGTGCCAAGATCCTGGTCGCCGGCGACAATTTCGGCTGCGGC
TCGAGCCGTGAGCATGCCCCGTGGGCGCTGCTCGATTTCGGCATCCGCTGCGTGATCTCGACCTCGTTCGGCGACATTTT
CTACAACAACTGCTTCAAGAACGGCGTGCTGCCGATCCGGGTGTCGCAGGCCGATCTCGACAAGCTGTTCGACGACGCAG
AGCGCGGCTCCAATGCCACGATGACGATCGACCTGCAGGCGCAGGAAATCCGCGGTCCCGACGGCGGCGCGATCAAGTTC
GAGATCGACCCGTTCCGCAAGCACTGCCTGATCAACGGTCTCGACGACATCGGCCTGACGCTGGAAAAGAAGCCGTCGAT
CGACGCCTACGAGACTAAGCTGAAGACCGAGCGCGCCTGGGCGTAA

Upstream 100 bases:

>100_bases
CTGGCCGAGGATTGAGCCTGCCGCGCGCGGCTCTGCGGTTTGCCGGCTTGACCGCCGGCCAAGGGGCGTTAAGACCATTC
CAAACCCCGGAGACCTGACG

Downstream 100 bases:

>100_bases
CGCCCCGGTTTCCTCGCTTGGCAGGAAGACGCGCGGCGGACCGGTGGCCCCGCTTGCGGTCTGTCGGAAAGTTCGCCGAT
CAAGCTTCGTGATGGCCGGG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFILNKPAYRGAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIRVSQADLDKLFDDAERGSNATMTIDLQAQEIRGPDGGAIKF
EIDPFRKHCLINGLDDIGLTLEKKPSIDAYETKLKTERAWA

Sequences:

>Translated_201_residues
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFILNKPAYRGAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIRVSQADLDKLFDDAERGSNATMTIDLQAQEIRGPDGGAIKF
EIDPFRKHCLINGLDDIGLTLEKKPSIDAYETKLKTERAWA
>Mature_201_residues
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFILNKPAYRGAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIRVSQADLDKLFDDAERGSNATMTIDLQAQEIRGPDGGAIKF
EIDPFRKHCLINGLDDIGLTLEKKPSIDAYETKLKTERAWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=50.5154639175258, Blast_Score=209, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321429, Length=205, Percent_Identity=58.0487804878049, Blast_Score=224, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22324; Mature: 22324

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFIL
CCCCCCCCCCCCCEEEEECCCCEECCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEE
NKPAYRGAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIR
ECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHCCEEEEE
VSQADLDKLFDDAERGSNATMTIDLQAQEIRGPDGGAIKFEIDPFRKHCLINGLDDIGLT
ECHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCEEEEEECHHHHHHHHCCCCCCCEE
LEKKPSIDAYETKLKTERAWA
EECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFIL
CCCCCCCCCCCCCEEEEECCCCEECCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEE
NKPAYRGAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIR
ECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHCCEEEEE
VSQADLDKLFDDAERGSNATMTIDLQAQEIRGPDGGAIKFEIDPFRKHCLINGLDDIGLT
ECHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCEEEEEECHHHHHHHHCCCCCCCEE
LEKKPSIDAYETKLKTERAWA
EECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]