Click here to switch to the map view.

The map label for this gene is cynT [H]

Identifier: 192288662

GI number: 192288662

Start: 249839

End: 250483

Strand: Reverse

Name: cynT [H]

Synonym: Rpal_0231

Alternate gene names: 192288662

Gene position: 250483-249839 (Counterclockwise)

Preceding gene: 192288664

Following gene: 192288659

Centisome position: 4.36

GC content: 64.03

Gene sequence:

>645_bases
ATGACATCCTTTCCGAAGTCACTGATCGACGGTTACGAGGCGTTCCGGACGCAGCGGCTGCCGACCGAGCAGAGCCGCTT
CCGCGAACTCTCCGAGCGCGGTCAGTCGCCGGAAGTGATGGTGATCGGCTGCTGCGACAGCCGGGTCTCGCCCGAGGTGA
TCTTCGACGCCGGACCGGGCGAAATGTTCGTGGTGCGCAATGTCGCCAATCTGGTGCCGGTGTACGAGCCGGACGGCGGC
GCCCATGGCGTCTCGGCGGCGCTGGAGTTCGCCGTGCAAGTGCTGAGGGTGAAGCACATCGTGGTGCTCGGCCACGCGCT
CTGCGGCGGCATCAAGGCGTTCACCGACAAGACCCCGCCGCTGACCGAAAGCGACTTCATCGGCCGCTGGATGCAGATGT
TCACCAAGCCGGGCGAAGTGGTCGAAAAACGCGACCACGAAACCGTGCAGGACTTCCGCACCCGGATCGAAAAGGCCGCG
GTGTTCCGCAGCATCGAGAACCTGATGACGTTCCCCTGCGTCAAGATCCTGGTGGAGCGCGGCAAGCTGCAACTCCACGG
CGCCTATTTCGGCGTCGCCGACGGCGATCTGTTCGTGCTCGATCAAGAGAACAAGCAGTTCGTGCCGGCCGCGAAAGTCG
TGTGA

Upstream 100 bases:

>100_bases
CCTGTGATTATGCGAGCGGCATCAGGCCGCGCGCGTCGCGATGGCGGTATCGTTCGCCGACGCGCCTGACCCCCGCGCCC
CGAAGGAGGGAACTGCGCCG

Downstream 100 bases:

>100_bases
GAGCAGACGCGACGTCATGGCCGGGGCTCGACCCGGCAATCCATCGTTTGGAAGCTAATCAGCTAATTGTTCACTGCGCT
TCGCGCGGGATGGATGCGCG

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTSFPKSLIDGYEAFRTQRLPTEQSRFRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPGEMFVVRNVANLVPVYEPDGG
AHGVSAALEFAVQVLRVKHIVVLGHALCGGIKAFTDKTPPLTESDFIGRWMQMFTKPGEVVEKRDHETVQDFRTRIEKAA
VFRSIENLMTFPCVKILVERGKLQLHGAYFGVADGDLFVLDQENKQFVPAAKVV

Sequences:

>Translated_214_residues
MTSFPKSLIDGYEAFRTQRLPTEQSRFRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPGEMFVVRNVANLVPVYEPDGG
AHGVSAALEFAVQVLRVKHIVVLGHALCGGIKAFTDKTPPLTESDFIGRWMQMFTKPGEVVEKRDHETVQDFRTRIEKAA
VFRSIENLMTFPCVKILVERGKLQLHGAYFGVADGDLFVLDQENKQFVPAAKVV
>Mature_213_residues
TSFPKSLIDGYEAFRTQRLPTEQSRFRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPGEMFVVRNVANLVPVYEPDGGA
HGVSAALEFAVQVLRVKHIVVLGHALCGGIKAFTDKTPPLTESDFIGRWMQMFTKPGEVVEKRDHETVQDFRTRIEKAAV
FRSIENLMTFPCVKILVERGKLQLHGAYFGVADGDLFVLDQENKQFVPAAKVV

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=206, Percent_Identity=33.9805825242718, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1786318, Length=194, Percent_Identity=30.4123711340206, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25147564, Length=236, Percent_Identity=24.5762711864407, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324292, Length=181, Percent_Identity=30.939226519337, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23831; Mature: 23700

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFPKSLIDGYEAFRTQRLPTEQSRFRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCC
EMFVVRNVANLVPVYEPDGGAHGVSAALEFAVQVLRVKHIVVLGHALCGGIKAFTDKTPP
CEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
LTESDFIGRWMQMFTKPGEVVEKRDHETVQDFRTRIEKAAVFRSIENLMTFPCVKILVER
CCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKLQLHGAYFGVADGDLFVLDQENKQFVPAAKVV
CCEEEEEEEEEECCCCEEEEECCCCCCCCHHCCC
>Mature Secondary Structure 
TSFPKSLIDGYEAFRTQRLPTEQSRFRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPG
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCC
EMFVVRNVANLVPVYEPDGGAHGVSAALEFAVQVLRVKHIVVLGHALCGGIKAFTDKTPP
CEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
LTESDFIGRWMQMFTKPGEVVEKRDHETVQDFRTRIEKAAVFRSIENLMTFPCVKILVER
CCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKLQLHGAYFGVADGDLFVLDQENKQFVPAAKVV
CCEEEEEEEEEECCCCEEEEECCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]