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The map label for this gene is tesA [H]

Identifier: 192288623

GI number: 192288623

Start: 208301

End: 208915

Strand: Reverse

Name: tesA [H]

Synonym: Rpal_0190

Alternate gene names: 192288623

Gene position: 208915-208301 (Counterclockwise)

Preceding gene: 192288628

Following gene: 192288622

Centisome position: 3.64

GC content: 67.15

Gene sequence:

>615_bases
ATGGCGGCGCTGCCTGGACAAACCGCGCGGGCCGAGACGGCCGCCAAGCCGCTGAAGCTGGTGGTGCTCGGGGACTCGCT
GACCGCCGGCTACGGCCTGCCGGCCGCCGATGCGTTTCCGGCCAAGCTGCAAAAGGCACTCAAGGCCAAAGGCATCGACA
CCGAGATGGTGAACGCTGGCGTATCCGGTGATACCGCCAGCGGCGGCCTGCAGCGGCTCGACTGGTCGGTCCCCCCTGAT
GCGGACGCGGTCATCGTCGAGCTCGGCGCCAACGATGCGCTGCGCGGCACCGATCCGAAGGTCCCGCGCAAGGCGCTGGA
CGAGATCTTGAAGCGGTTGAAGGCGCGCAATCTGCCGGTGCTGCTCGCCGGCATGCTGGCGCCGCCGAATTACGGCAAAG
ACTACGCCGCGCAGTTCGACGCGATCTATCCGGACCTTGCCAAGCAGTACGACGTGCCGCTGTATCCGTTCTTCCTCGAC
GGCGTCGCGGCGGTGTCGAATCTCAATCAGGCCGACGGCATCCATCCGACCGCGGCCGGCGTCGACATCATCGTCGAGCG
GATTTTGCCCAAGGTTGAGGCGCTGCTTCGCCCGCTCCTGGTGCAGAGCCGCTGA

Upstream 100 bases:

>100_bases
GGGTTCAATGAGACTGTCCATGGCTGCCTCATATGGCTATTCAATCGTGGCGGTCGAGGGGTGGGCGCGCACGATCGCGC
TGGTCCTGGGACTTGGAATA

Downstream 100 bases:

>100_bases
GAAAACGGCAATCCGGGCTTAAGGGTAGGATTGCCCTCAGCTTTTTCGGCTCTGCGCGGGCTCCACTGTAGCGCTGTTAC
ACAAACGCGCTACACAATCG

Product: G-D-S-L family lipolytic protein

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MAALPGQTARAETAAKPLKLVVLGDSLTAGYGLPAADAFPAKLQKALKAKGIDTEMVNAGVSGDTASGGLQRLDWSVPPD
ADAVIVELGANDALRGTDPKVPRKALDEILKRLKARNLPVLLAGMLAPPNYGKDYAAQFDAIYPDLAKQYDVPLYPFFLD
GVAAVSNLNQADGIHPTAAGVDIIVERILPKVEALLRPLLVQSR

Sequences:

>Translated_204_residues
MAALPGQTARAETAAKPLKLVVLGDSLTAGYGLPAADAFPAKLQKALKAKGIDTEMVNAGVSGDTASGGLQRLDWSVPPD
ADAVIVELGANDALRGTDPKVPRKALDEILKRLKARNLPVLLAGMLAPPNYGKDYAAQFDAIYPDLAKQYDVPLYPFFLD
GVAAVSNLNQADGIHPTAAGVDIIVERILPKVEALLRPLLVQSR
>Mature_203_residues
AALPGQTARAETAAKPLKLVVLGDSLTAGYGLPAADAFPAKLQKALKAKGIDTEMVNAGVSGDTASGGLQRLDWSVPPDA
DAVIVELGANDALRGTDPKVPRKALDEILKRLKARNLPVLLAGMLAPPNYGKDYAAQFDAIYPDLAKQYDVPLYPFFLDG
VAAVSNLNQADGIHPTAAGVDIIVERILPKVEALLRPLLVQSR

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=178, Percent_Identity=42.1348314606742, Blast_Score=130, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21487; Mature: 21356

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALPGQTARAETAAKPLKLVVLGDSLTAGYGLPAADAFPAKLQKALKAKGIDTEMVNAG
CCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC
VSGDTASGGLQRLDWSVPPDADAVIVELGANDALRGTDPKVPRKALDEILKRLKARNLPV
CCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
LLAGMLAPPNYGKDYAAQFDAIYPDLAKQYDVPLYPFFLDGVAAVSNLNQADGIHPTAAG
EEEHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCCCHHH
VDIIVERILPKVEALLRPLLVQSR
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AALPGQTARAETAAKPLKLVVLGDSLTAGYGLPAADAFPAKLQKALKAKGIDTEMVNAG
CCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC
VSGDTASGGLQRLDWSVPPDADAVIVELGANDALRGTDPKVPRKALDEILKRLKARNLPV
CCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
LLAGMLAPPNYGKDYAAQFDAIYPDLAKQYDVPLYPFFLDGVAAVSNLNQADGIHPTAAG
EEEHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCCCHHH
VDIIVERILPKVEALLRPLLVQSR
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]