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The map label for this gene is mdh1 [H]

Identifier: 192288620

GI number: 192288620

Start: 205175

End: 206143

Strand: Reverse

Name: mdh1 [H]

Synonym: Rpal_0187

Alternate gene names: 192288620

Gene position: 206143-205175 (Counterclockwise)

Preceding gene: 192288621

Following gene: 192288619

Centisome position: 3.59

GC content: 65.12

Gene sequence:

>969_bases
ATGGCGCGCGATAAAATTGCTTTGATTGGCTCCGGCCAGATCGGCGGTACGCTGGCACATTTGGTCGGGTTGAAAGAGCT
CGGCGACGTGGTGCTGTTCGACATTGCCGAGGGCGTGCCGCAGGGCAAGGCGCTCGACATCGCGGAGTCCTCGCCGGTCG
ACGGTTTCGACTCCAAGCTGACCGGTGCCAACTCCTACGAGGCGATCGAAGGCGCCCGCGTGGTGATCGTCACCGCCGGC
GTGCCGCGCAAGCCGGGCATGAGCCGTGACGATCTGCTCAGCATCAACCTCAAGGTGATGGAGCAGGTCGGCGCCGGCAT
CAAGAAGTACGCGCCCGACGCGTTCGTGATCTGCATCACCAACCCGCTCGACGCGATGGTCTGGGCGCTGCAGAAGGCCT
CGGGCCTGCCGGCCAAGAAGGTCGTCGGCATGGCCGGCGTGCTAGACTCGGCGCGGTTCCGTTACTTCCTGGCCGACGAG
TTCAACGTGTCGGTCGAAGACGTCACCGCCTTCGTGCTCGGCGGCCACGGTGATACCATGGTGCCGCTGGTGAAGTACTC
GACCGTCGCCGGAATTCCGCTGCCCGACCTGGTCAAGATGGGCTGGACCTCGCAGGCGCGCCTCGACGAGATCGTCGACC
GCACCCGCAACGGCGGCGCCGAGATCGTCAACCTGCTGAAGACCGGCTCGGCGTTCTACGCCCCGGCGTCGTCGGCGATC
GCGATGGCCGAGAGCTATCTGAAGGACAAGAAGCGGGTGGTGCCGGTCGCGGCCCACCTCAACGGCGAATACGGCGTCAA
GGACATGTATGTCGGCGTTCCGGTGGTGATCGGCGACAAGGGCGTCGAGCGCATCGTCGAGATCGAGCTGGCCGGCAAGG
ACAAGGAAGCGTTCGACAAGTCGGTCGCTGCCGTTCAGGGTCTGGTCGAGGCCTGCAAGAAGATTGCGCCCGATCTGCTC
GGTCGCTGA

Upstream 100 bases:

>100_bases
AATGGGCGCTCGCCGACTTGAACGCATGGGGCGAAAGCGATAACCACCCAAGCGGATTTTCCACCCCTCCCCACCCACGG
GCTCAAAGGACTGCTTTCCC

Downstream 100 bases:

>100_bases
GGAACGTCTAACCAAACTGGCTGCGCCGGCTCCTTAAGGACCGGCGCAGCATCGCGGTTCAACCGGCTGGATGCGATCTG
GCGAGGGGCAATTCCATGAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MARDKIALIGSGQIGGTLAHLVGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDSKLTGANSYEAIEGARVVIVTAG
VPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADE
FNVSVEDVTAFVLGGHGDTMVPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPASSAI
AMAESYLKDKKRVVPVAAHLNGEYGVKDMYVGVPVVIGDKGVERIVEIELAGKDKEAFDKSVAAVQGLVEACKKIAPDLL
GR

Sequences:

>Translated_322_residues
MARDKIALIGSGQIGGTLAHLVGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDSKLTGANSYEAIEGARVVIVTAG
VPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADE
FNVSVEDVTAFVLGGHGDTMVPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPASSAI
AMAESYLKDKKRVVPVAAHLNGEYGVKDMYVGVPVVIGDKGVERIVEIELAGKDKEAFDKSVAAVQGLVEACKKIAPDLL
GR
>Mature_321_residues
ARDKIALIGSGQIGGTLAHLVGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDSKLTGANSYEAIEGARVVIVTAGV
PRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADEF
NVSVEDVTAFVLGGHGDTMVPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPASSAIA
MAESYLKDKKRVVPVAAHLNGEYGVKDMYVGVPVVIGDKGVERIVEIELAGKDKEAFDKSVAAVQGLVEACKKIAPDLLG
R

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=30.1639344262295, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=30.1639344262295, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI9257228, Length=311, Percent_Identity=31.8327974276527, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4504973, Length=311, Percent_Identity=31.8327974276527, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=29.1666666666667, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=29.1666666666667, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI15082234, Length=306, Percent_Identity=27.1241830065359, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=29.3729372937294, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=29.3729372937294, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=31.5, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=31.5, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=30.2564102564103, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI21735621, Length=298, Percent_Identity=27.8523489932886, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI103472011, Length=318, Percent_Identity=21.6981132075472, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789632, Length=326, Percent_Identity=30.6748466257669, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535107, Length=246, Percent_Identity=27.6422764227642, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=315, Percent_Identity=30.4761904761905, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=302, Percent_Identity=29.1390728476821, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=243, Percent_Identity=29.2181069958848, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=28.0528052805281, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45550422, Length=320, Percent_Identity=25, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24647881, Length=303, Percent_Identity=29.7029702970297, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24663595, Length=294, Percent_Identity=28.9115646258503, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663599, Length=292, Percent_Identity=28.0821917808219, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33969; Mature: 33838

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDKIALIGSGQIGGTLAHLVGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDSKL
CCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEECCCCCCCCCCCCCC
TGANSYEAIEGARVVIVTAGVPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICIT
CCCCCCHHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADEFNVSVEDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEECCCCCCE
VPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPASSAI
EEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCHHHH
AMAESYLKDKKRVVPVAAHLNGEYGVKDMYVGVPVVIGDKGVERIVEIELAGKDKEAFDK
HHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCHHHHHH
SVAAVQGLVEACKKIAPDLLGR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARDKIALIGSGQIGGTLAHLVGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDSKL
CCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEECCCCCCCCCCCCCC
TGANSYEAIEGARVVIVTAGVPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICIT
CCCCCCHHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADEFNVSVEDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEECCCCCCE
VPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPASSAI
EEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCHHHH
AMAESYLKDKKRVVPVAAHLNGEYGVKDMYVGVPVVIGDKGVERIVEIELAGKDKEAFDK
HHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCHHHHHH
SVAAVQGLVEACKKIAPDLLGR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA