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The map label for this gene is appA [H]

Identifier: 192288539

GI number: 192288539

Start: 115283

End: 116884

Strand: Reverse

Name: appA [H]

Synonym: Rpal_0106

Alternate gene names: 192288539

Gene position: 116884-115283 (Counterclockwise)

Preceding gene: 192288540

Following gene: 192288538

Centisome position: 2.03

GC content: 62.86

Gene sequence:

>1602_bases
ATGATGTTGACGAAGCGATCCTTTGTAGTGGGAGCCCTCGGCGGCATTGCGCTGATGGGTCTGCCGCTCGAAACCAAAGC
GGAAGGTGCGGGGAGCGGCGGAACGCTGGTGATCGGTTCGACGCAGGTGCCGCGGCATTTCAACGGCGCCGTCCAGTCCG
GTATCGCCACCGCGCTCCCGAGCACGCAGATCTTCGCCAGTCCGCTGCGCTACGACGACAACTGGAACCCGCAGCCCTAT
CTCGCCGAGTCCTGGGACGTCTCGAAGGACGGGCTGACGGTGACGCTGAAGCTGGTCAAGAACGCCACCTTCCACGACGG
CAAGCCGGTGACTTCCGAGGACGTCGCGTTCTCGATCATGACCATCAAGGCCAATCATCCGTTCAAGACGATGCTGGCCG
CGGTCGAGAGCGTCGATACGCCGGATCCGCATACGGCGGTGATCAAGCTGGCGCATCCGCACCCGGCGCTGCTGCTGGCG
ATGTCGCCTGCATTGATGCCGATCCTGCCGAAACACGTCTATGGCGACGGCCAGGACGTCAAATCGCATCCGGCCAACCT
GAAGCCGATCGGCTCCGGTCCCTACAAGCTCACCGAGTACAAGCAGGGCGACTACTACACGCTGGAGAAGTTCGACAAAT
TCTTCATCCCGGGGCGGCCGAAGCTCGACAAGATCGTGGTGCGGCTGATTTCGGATCCGAATGCCCTGATGGTGTCGGCC
GAGCGCGGCGATGTTCATATGGTGCCCTACTTCACCGGCGTGCGCGACATTGAGCGGCTGGAAAAGGCGCCGAACGTCGT
CGTCACCGACAAGGGCTTTGCCGGCATCGGTGCGCTGAACTGGCTCGCCTTCAACACCAAGAAGAAGCCGCTCGACGACG
TTCGCGTCCGTCAGGCGATCGGCTATGCGGCGAACCGCGAGTTCATCGTCAAGAAGCTGATGGGCGGCAAAGCCTTGCCC
TCGACCGGACCGATCGCGCCGGGCTCGCCGCTGGAAGAGAAGAACGTCGAGCAGTACAAATTCGACATCGCCAAGGCCAA
CAAGCTGCTCGACGAGGCTGGGCTCAAGCCGGACGGCTCCGGCGTGCGCACCACGCTGACGATCGACTACATCCCCGGCA
ACGACGAGCAGCAGCGCAACGTCGCCGAATATCTGCGTTCGGCGCTGAAGCGGGTCGGGATCAATCTCGAGGTTCGCGCC
GCTCCCGACTTCCCGACCTGGGCCCAGCGTGTCGCCAGTTTCGACTTCGACATGACGATGGACACCGTGTTCAACTGGGG
CGACCCGGTGATCGGCGTCGACCGGACTTATCTGAGCTCGAACATCCGCAAGGGCATCATCTGGTCGAACACCCAGCAGT
ACGCCAATCCGAAGGTCGACGAGATCCTCGGCCAGGCCGCCCAGGAGAGCTCGCCGGACAAGCGCAAGGCGCTGTATTCG
GAGTTCCAGAAGATCGTCGTCGAAGATGCGCCGATCTTCTACATCAACGCCACTCCGTACCACACCTCGTTCGCCAAGGG
GCTCGGCAACCTGCCGACCACGGTGTGGGGCGTCGCCTCGCCGCTCGACGAGCTGTACTGGGTGACTCCGCCGAAGAACT
GA

Upstream 100 bases:

>100_bases
CGCTGTCGTGAGGCCCACCCTGCCAGTTTCTCGCCGCGAAAGCCATGCGGCTTTCCACGTCCTGCCTCGCCCATCCTTTC
TTGCAGCGGAGTTCTGACCG

Downstream 100 bases:

>100_bases
TCCGCCAATCTCGATCGACAGAGCATCAATGGCTCTCCTCACCCATCTGTTCGGAAAACTCGCCAACGCCGTCGCGCTGC
TGCTGGCGGTGCTGGTGATG

Product: family 5 extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MMLTKRSFVVGALGGIALMGLPLETKAEGAGSGGTLVIGSTQVPRHFNGAVQSGIATALPSTQIFASPLRYDDNWNPQPY
LAESWDVSKDGLTVTLKLVKNATFHDGKPVTSEDVAFSIMTIKANHPFKTMLAAVESVDTPDPHTAVIKLAHPHPALLLA
MSPALMPILPKHVYGDGQDVKSHPANLKPIGSGPYKLTEYKQGDYYTLEKFDKFFIPGRPKLDKIVVRLISDPNALMVSA
ERGDVHMVPYFTGVRDIERLEKAPNVVVTDKGFAGIGALNWLAFNTKKKPLDDVRVRQAIGYAANREFIVKKLMGGKALP
STGPIAPGSPLEEKNVEQYKFDIAKANKLLDEAGLKPDGSGVRTTLTIDYIPGNDEQQRNVAEYLRSALKRVGINLEVRA
APDFPTWAQRVASFDFDMTMDTVFNWGDPVIGVDRTYLSSNIRKGIIWSNTQQYANPKVDEILGQAAQESSPDKRKALYS
EFQKIVVEDAPIFYINATPYHTSFAKGLGNLPTTVWGVASPLDELYWVTPPKN

Sequences:

>Translated_533_residues
MMLTKRSFVVGALGGIALMGLPLETKAEGAGSGGTLVIGSTQVPRHFNGAVQSGIATALPSTQIFASPLRYDDNWNPQPY
LAESWDVSKDGLTVTLKLVKNATFHDGKPVTSEDVAFSIMTIKANHPFKTMLAAVESVDTPDPHTAVIKLAHPHPALLLA
MSPALMPILPKHVYGDGQDVKSHPANLKPIGSGPYKLTEYKQGDYYTLEKFDKFFIPGRPKLDKIVVRLISDPNALMVSA
ERGDVHMVPYFTGVRDIERLEKAPNVVVTDKGFAGIGALNWLAFNTKKKPLDDVRVRQAIGYAANREFIVKKLMGGKALP
STGPIAPGSPLEEKNVEQYKFDIAKANKLLDEAGLKPDGSGVRTTLTIDYIPGNDEQQRNVAEYLRSALKRVGINLEVRA
APDFPTWAQRVASFDFDMTMDTVFNWGDPVIGVDRTYLSSNIRKGIIWSNTQQYANPKVDEILGQAAQESSPDKRKALYS
EFQKIVVEDAPIFYINATPYHTSFAKGLGNLPTTVWGVASPLDELYWVTPPKN
>Mature_533_residues
MMLTKRSFVVGALGGIALMGLPLETKAEGAGSGGTLVIGSTQVPRHFNGAVQSGIATALPSTQIFASPLRYDDNWNPQPY
LAESWDVSKDGLTVTLKLVKNATFHDGKPVTSEDVAFSIMTIKANHPFKTMLAAVESVDTPDPHTAVIKLAHPHPALLLA
MSPALMPILPKHVYGDGQDVKSHPANLKPIGSGPYKLTEYKQGDYYTLEKFDKFFIPGRPKLDKIVVRLISDPNALMVSA
ERGDVHMVPYFTGVRDIERLEKAPNVVVTDKGFAGIGALNWLAFNTKKKPLDDVRVRQAIGYAANREFIVKKLMGGKALP
STGPIAPGSPLEEKNVEQYKFDIAKANKLLDEAGLKPDGSGVRTTLTIDYIPGNDEQQRNVAEYLRSALKRVGINLEVRA
APDFPTWAQRVASFDFDMTMDTVFNWGDPVIGVDRTYLSSNIRKGIIWSNTQQYANPKVDEILGQAAQESSPDKRKALYS
EFQKIVVEDAPIFYINATPYHTSFAKGLGNLPTTVWGVASPLDELYWVTPPKN

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=426, Percent_Identity=28.4037558685446, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789966, Length=525, Percent_Identity=24.952380952381, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1787762, Length=440, Percent_Identity=26.1363636363636, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789887, Length=486, Percent_Identity=22.0164609053498, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081878, Length=500, Percent_Identity=24.4, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787551, Length=544, Percent_Identity=21.5073529411765, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87082063, Length=402, Percent_Identity=24.6268656716418, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58406; Mature: 58406

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLTKRSFVVGALGGIALMGLPLETKAEGAGSGGTLVIGSTQVPRHFNGAVQSGIATALP
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHCC
STQIFASPLRYDDNWNPQPYLAESWDVSKDGLTVTLKLVKNATFHDGKPVTSEDVAFSIM
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEE
TIKANHPFKTMLAAVESVDTPDPHTAVIKLAHPHPALLLAMSPALMPILPKHVYGDGQDV
EEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCHHHHHCHHHHCCCCCCH
KSHPANLKPIGSGPYKLTEYKQGDYYTLEKFDKFFIPGRPKLDKIVVRLISDPNALMVSA
HCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEE
ERGDVHMVPYFTGVRDIERLEKAPNVVVTDKGFAGIGALNWLAFNTKKKPLDDVRVRQAI
CCCCEEEEEHHHCHHHHHHHHHCCCEEEECCCCCCCCHHEEEEECCCCCCHHHHHHHHHH
GYAANREFIVKKLMGGKALPSTGPIAPGSPLEEKNVEQYKFDIAKANKLLDEAGLKPDGS
HHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GVRTTLTIDYIPGNDEQQRNVAEYLRSALKRVGINLEVRAAPDFPTWAQRVASFDFDMTM
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCH
DTVFNWGDPVIGVDRTYLSSNIRKGIIWSNTQQYANPKVDEILGQAAQESSPDKRKALYS
HHHHCCCCCEEECCHHHHHHCCCCCEEECCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
EFQKIVVEDAPIFYINATPYHTSFAKGLGNLPTTVWGVASPLDELYWVTPPKN
HHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHCCHHHCEEECCCCC
>Mature Secondary Structure
MMLTKRSFVVGALGGIALMGLPLETKAEGAGSGGTLVIGSTQVPRHFNGAVQSGIATALP
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHCC
STQIFASPLRYDDNWNPQPYLAESWDVSKDGLTVTLKLVKNATFHDGKPVTSEDVAFSIM
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEE
TIKANHPFKTMLAAVESVDTPDPHTAVIKLAHPHPALLLAMSPALMPILPKHVYGDGQDV
EEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCHHHHHCHHHHCCCCCCH
KSHPANLKPIGSGPYKLTEYKQGDYYTLEKFDKFFIPGRPKLDKIVVRLISDPNALMVSA
HCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEE
ERGDVHMVPYFTGVRDIERLEKAPNVVVTDKGFAGIGALNWLAFNTKKKPLDDVRVRQAI
CCCCEEEEEHHHCHHHHHHHHHCCCEEEECCCCCCCCHHEEEEECCCCCCHHHHHHHHHH
GYAANREFIVKKLMGGKALPSTGPIAPGSPLEEKNVEQYKFDIAKANKLLDEAGLKPDGS
HHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GVRTTLTIDYIPGNDEQQRNVAEYLRSALKRVGINLEVRAAPDFPTWAQRVASFDFDMTM
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCH
DTVFNWGDPVIGVDRTYLSSNIRKGIIWSNTQQYANPKVDEILGQAAQESSPDKRKALYS
HHHHCCCCCEEECCHHHHHHCCCCCEEECCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
EFQKIVVEDAPIFYINATPYHTSFAKGLGNLPTTVWGVASPLDELYWVTPPKN
HHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHCCHHHCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]