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The map label for this gene is pheT [H]

Identifier: 192288469

GI number: 192288469

Start: 40444

End: 42855

Strand: Reverse

Name: pheT [H]

Synonym: Rpal_0036

Alternate gene names: 192288469

Gene position: 42855-40444 (Counterclockwise)

Preceding gene: 192288470

Following gene: 192288467

Centisome position: 0.75

GC content: 67.12

Gene sequence:

>2412_bases
ATGAAACTCACCCTCTCCTGGCTGAAAGATCATCTCGACACCGACGAGCCGCTGGAAAAGCTCGCCGACAAGCTCACCAT
GATCGGGCTCGAAGTCGAGGGCATCGAGGACAAGGCGAAGGTGCTGGCGCCGTTCACCATCGCCAAGGTGCTAACGGCCG
CCAAGCATCCCAACGCCGACAAGCTGCAGGTCTGCACGGTCGACGTCGGTGACGGGGCTGCGCCGGTGCAGGTGGTGTGC
GGCGCGCCGAATGCGCGCGCCGGCCTCGTCACGGTGTTCGCGCCGCCCGGCACATATATTCCGGCCAAGGACTTCACGCT
CGGCATCGGCAATATTCGCGGCGTCGAGAGCCGCGGCATGCTGTGCTCGGCCGCTGAATTGCAGATCTCCGAGGATCACG
ACGGCATCATCGAGCTGCCGGCCGATGCGCCGGTCGGTGCCGCCTATGCGGCCTGGGCCGGGCTCGGCGATCCGGTGCTC
GACATCAACCTGACGCCGAACCGGCAGGACTGCGCCGGCGTGCACGGCATCGCGCGCGATCTCGCCGCCGCCGACATGGG
CAAGTTCAAGGACCCGGGTATCAAGCCGATCAAGGGCGAATTCCCGTGCCCGGTCAGTGTCACTGTCGAAGACAGCACGC
TGTGCCCCGGCTTCGCGCTGCGGCTGGTGAAGGGCGTCAAGAACGGCCCGTCGCCGGAATGGCTGCAGAAGCGCCTGACT
GCGATCGGACTGCGCCCGATCAACGCGTTGGTCGACATCACCAACTACCTGACATTCGATCGCTCGCGCCCGCTGCACGT
GTTCGACGCCGCCAAGGTGAAGGGCAATCTGGTGGTGCGCCGCGCCAAGGACGGCGAGACGCTGCTCGCGCTCGACGGCC
GCACCTACACGCTGGATTCGTCAGTCTGCGTGATCGCCGACGATCACGGCGTCGAATCGCTCGCCGGCATCATGGGGGGT
GAGCTGTCCGGCTGCTCGGCCGAGACCACCGACGTGCTGATCGAATCGGCGCTGTGGAACGAGATCAACATCGCCCAGTC
GGGCCGCAAGCTCGGCATCAACACCGATGCGCGCTACCGCTTCGAGCGCGGCGTCGATCCAGCCTTCATGCTGCCTGGGC
TCGAGCTTGCCACCAAGCTGGTGATGGAGTTCTGCGGCGGCACGCCGTCGGAGGCGGTGGTGGTCGGCAATCCGTTCGCC
GACGACAAGATCATCGACTTCCCGCTCGCCGAGGTGAAGCGTCTGGCCGGCATCGAAGTATCGCTCACCGAGATCCGCCG
TATCCTCAATCACCTCGGCTTCACGGTCGTCGGGCAGGCGCCGGTGGTGAAGGTCGCGGTGCCGTCGTGGCGCAGCGACG
TTCATGGCAAGGCCGATATCGTCGAGGAAATCGTCCGGATCGTCGGCGTCGACAAGGTGCCGCTGACGCCGTTCGAGCGC
GGCGATGCCCCGCGCAAGCCGGTACTCACCCAGATCCAGAATCGCACCCGCCGCGCCAAGCGCGCGCTGGCGGCGCGCGG
CCTGACCGAGGCGGTGACCTGGTCGTTCATCTCCAAGCCGTTCGCCGAAGCGTTCGGCGGCGGCCAGCCGGAGCTGGCGC
TCGCCAATCCGATCGCGTCCGATCTGTCCGACATGCGGCCGAGCCTGCTGCCCGGCCTGATCGCCGCCGCACAGGCCAAC
GCCGATCGCGGTTCGCCCGATCTGTCGCTGTTCGAAGTCGGGCAGGTGTTCAAGGGCGACCGGCCGCAGGATCAATTCAT
GGCTGCGAGCGGCGTCCGCCGCGGTGTCGCGTCCTCCGCGGGCCTCGGCCGGCATTGGTCGGGCTCGGCGCAGGCGACCG
CGCTCGATGCCAAGGCCGATGCGTTCGCGGTGCTGGCTGCGGCCGGTGCGCCGATGGCGGGGCTGCAGATTGCCACCAAC
AAGCTGCCGGCCTGGCTGCATCCGGGCCGCTCCGGCGCGATCCAGATCGGGCCGCAGAACGTGCTCGGCTATTTCGGCGA
GCTGCATCCGCGGGTGCTCGAACAGCTCGGGGCGGATGGCCCGCTGGTGGCGTTCGAAGTGATCTTGGAAAAGATCCCCG
ATCCGAAGCAGCGGCCGACCCGCGCCAAGCCGGCGCTCGAACTGTCTGCGTTCCACCCGGTGTCGCGCGACTTCGCCTTC
ATCGTCGATCGCAAGGTCGCGGTTGCCGATATCGTCCGCGCCGCGCAGGGCGTCGACAAGAAGCTGATCACCTCGGTCAG
CGTGTTCGACGTCTATGAAGGCAAGGGCATCGATCCTGACAAAAAGTCGGTGGCGATCGCCGTGACGCTGCAGCCGCGCG
ACAAGACCATGACCGATCAGGAGATCGAAGCGGTCGCGGCCAAGATCGTGGCGGAAGTCACCAAGAAGACCGGCGGCAGC
CTGCGCGGCTGA

Upstream 100 bases:

>100_bases
CCCGCTTGCGGGAGAGGGGTGGGGTGAGGGCGACGCGGGCACCGTTTCGACGATTCAGCACGGCGCGTCTCTCCGCGCTG
CCAGACGGAAGTAACAACCG

Downstream 100 bases:

>100_bases
GGCCGCTCAGCGCCACCACGACGGCGTGCCGCTCGCGGTGGCGGGCGGGCGCTGCTCGCGCTGACGCTGCGGTTCGCGCC
GCGGCTTCTGTTTGCGTAGG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MKLTLSWLKDHLDTDEPLEKLADKLTMIGLEVEGIEDKAKVLAPFTIAKVLTAAKHPNADKLQVCTVDVGDGAAPVQVVC
GAPNARAGLVTVFAPPGTYIPAKDFTLGIGNIRGVESRGMLCSAAELQISEDHDGIIELPADAPVGAAYAAWAGLGDPVL
DINLTPNRQDCAGVHGIARDLAAADMGKFKDPGIKPIKGEFPCPVSVTVEDSTLCPGFALRLVKGVKNGPSPEWLQKRLT
AIGLRPINALVDITNYLTFDRSRPLHVFDAAKVKGNLVVRRAKDGETLLALDGRTYTLDSSVCVIADDHGVESLAGIMGG
ELSGCSAETTDVLIESALWNEINIAQSGRKLGINTDARYRFERGVDPAFMLPGLELATKLVMEFCGGTPSEAVVVGNPFA
DDKIIDFPLAEVKRLAGIEVSLTEIRRILNHLGFTVVGQAPVVKVAVPSWRSDVHGKADIVEEIVRIVGVDKVPLTPFER
GDAPRKPVLTQIQNRTRRAKRALAARGLTEAVTWSFISKPFAEAFGGGQPELALANPIASDLSDMRPSLLPGLIAAAQAN
ADRGSPDLSLFEVGQVFKGDRPQDQFMAASGVRRGVASSAGLGRHWSGSAQATALDAKADAFAVLAAAGAPMAGLQIATN
KLPAWLHPGRSGAIQIGPQNVLGYFGELHPRVLEQLGADGPLVAFEVILEKIPDPKQRPTRAKPALELSAFHPVSRDFAF
IVDRKVAVADIVRAAQGVDKKLITSVSVFDVYEGKGIDPDKKSVAIAVTLQPRDKTMTDQEIEAVAAKIVAEVTKKTGGS
LRG

Sequences:

>Translated_803_residues
MKLTLSWLKDHLDTDEPLEKLADKLTMIGLEVEGIEDKAKVLAPFTIAKVLTAAKHPNADKLQVCTVDVGDGAAPVQVVC
GAPNARAGLVTVFAPPGTYIPAKDFTLGIGNIRGVESRGMLCSAAELQISEDHDGIIELPADAPVGAAYAAWAGLGDPVL
DINLTPNRQDCAGVHGIARDLAAADMGKFKDPGIKPIKGEFPCPVSVTVEDSTLCPGFALRLVKGVKNGPSPEWLQKRLT
AIGLRPINALVDITNYLTFDRSRPLHVFDAAKVKGNLVVRRAKDGETLLALDGRTYTLDSSVCVIADDHGVESLAGIMGG
ELSGCSAETTDVLIESALWNEINIAQSGRKLGINTDARYRFERGVDPAFMLPGLELATKLVMEFCGGTPSEAVVVGNPFA
DDKIIDFPLAEVKRLAGIEVSLTEIRRILNHLGFTVVGQAPVVKVAVPSWRSDVHGKADIVEEIVRIVGVDKVPLTPFER
GDAPRKPVLTQIQNRTRRAKRALAARGLTEAVTWSFISKPFAEAFGGGQPELALANPIASDLSDMRPSLLPGLIAAAQAN
ADRGSPDLSLFEVGQVFKGDRPQDQFMAASGVRRGVASSAGLGRHWSGSAQATALDAKADAFAVLAAAGAPMAGLQIATN
KLPAWLHPGRSGAIQIGPQNVLGYFGELHPRVLEQLGADGPLVAFEVILEKIPDPKQRPTRAKPALELSAFHPVSRDFAF
IVDRKVAVADIVRAAQGVDKKLITSVSVFDVYEGKGIDPDKKSVAIAVTLQPRDKTMTDQEIEAVAAKIVAEVTKKTGGS
LRG
>Mature_803_residues
MKLTLSWLKDHLDTDEPLEKLADKLTMIGLEVEGIEDKAKVLAPFTIAKVLTAAKHPNADKLQVCTVDVGDGAAPVQVVC
GAPNARAGLVTVFAPPGTYIPAKDFTLGIGNIRGVESRGMLCSAAELQISEDHDGIIELPADAPVGAAYAAWAGLGDPVL
DINLTPNRQDCAGVHGIARDLAAADMGKFKDPGIKPIKGEFPCPVSVTVEDSTLCPGFALRLVKGVKNGPSPEWLQKRLT
AIGLRPINALVDITNYLTFDRSRPLHVFDAAKVKGNLVVRRAKDGETLLALDGRTYTLDSSVCVIADDHGVESLAGIMGG
ELSGCSAETTDVLIESALWNEINIAQSGRKLGINTDARYRFERGVDPAFMLPGLELATKLVMEFCGGTPSEAVVVGNPFA
DDKIIDFPLAEVKRLAGIEVSLTEIRRILNHLGFTVVGQAPVVKVAVPSWRSDVHGKADIVEEIVRIVGVDKVPLTPFER
GDAPRKPVLTQIQNRTRRAKRALAARGLTEAVTWSFISKPFAEAFGGGQPELALANPIASDLSDMRPSLLPGLIAAAQAN
ADRGSPDLSLFEVGQVFKGDRPQDQFMAASGVRRGVASSAGLGRHWSGSAQATALDAKADAFAVLAAAGAPMAGLQIATN
KLPAWLHPGRSGAIQIGPQNVLGYFGELHPRVLEQLGADGPLVAFEVILEKIPDPKQRPTRAKPALELSAFHPVSRDFAF
IVDRKVAVADIVRAAQGVDKKLITSVSVFDVYEGKGIDPDKKSVAIAVTLQPRDKTMTDQEIEAVAAKIVAEVTKKTGGS
LRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=817, Percent_Identity=35.4957160342717, Blast_Score=464, Evalue=1e-131,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85212; Mature: 85212

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTLSWLKDHLDTDEPLEKLADKLTMIGLEVEGIEDKAKVLAPFTIAKVLTAAKHPNAD
CEEEHHHHHHHCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
KLQVCTVDVGDGAAPVQVVCGAPNARAGLVTVFAPPGTYIPAKDFTLGIGNIRGVESRGM
CEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCC
LCSAAELQISEDHDGIIELPADAPVGAAYAAWAGLGDPVLDINLTPNRQDCAGVHGIARD
EEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
LAAADMGKFKDPGIKPIKGEFPCPVSVTVEDSTLCPGFALRLVKGVKNGPSPEWLQKRLT
HHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
AIGLRPINALVDITNYLTFDRSRPLHVFDAAKVKGNLVVRRAKDGETLLALDGRTYTLDS
HHCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCEEEEECC
SVCVIADDHGVESLAGIMGGELSGCSAETTDVLIESALWNEINIAQSGRKLGINTDARYR
CEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEECCCCCCCCH
FERGVDPAFMLPGLELATKLVMEFCGGTPSEAVVVGNPFADDKIIDFPLAEVKRLAGIEV
HHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCHHHHHHHCCCEE
SLTEIRRILNHLGFTVVGQAPVVKVAVPSWRSDVHGKADIVEEIVRIVGVDKVPLTPFER
EHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GDAPRKPVLTQIQNRTRRAKRALAARGLTEAVTWSFISKPFAEAFGGGQPELALANPIAS
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHH
DLSDMRPSLLPGLIAAAQANADRGSPDLSLFEVGQVFKGDRPQDQFMAASGVRRGVASSA
HHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
GLGRHWSGSAQATALDAKADAFAVLAAAGAPMAGLQIATNKLPAWLHPGRSGAIQIGPQN
CCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCEEEECCCCCCEECCCCCCEEEECHHH
VLGYFGELHPRVLEQLGADGPLVAFEVILEKIPDPKQRPTRAKPALELSAFHPVSRDFAF
HHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCEEE
IVDRKVAVADIVRAAQGVDKKLITSVSVFDVYEGKGIDPDKKSVAIAVTLQPRDKTMTDQ
EECCHHHHHHHHHHHHCCCHHHHHHEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHH
EIEAVAAKIVAEVTKKTGGSLRG
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLTLSWLKDHLDTDEPLEKLADKLTMIGLEVEGIEDKAKVLAPFTIAKVLTAAKHPNAD
CEEEHHHHHHHCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
KLQVCTVDVGDGAAPVQVVCGAPNARAGLVTVFAPPGTYIPAKDFTLGIGNIRGVESRGM
CEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCC
LCSAAELQISEDHDGIIELPADAPVGAAYAAWAGLGDPVLDINLTPNRQDCAGVHGIARD
EEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
LAAADMGKFKDPGIKPIKGEFPCPVSVTVEDSTLCPGFALRLVKGVKNGPSPEWLQKRLT
HHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
AIGLRPINALVDITNYLTFDRSRPLHVFDAAKVKGNLVVRRAKDGETLLALDGRTYTLDS
HHCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCEEEEECC
SVCVIADDHGVESLAGIMGGELSGCSAETTDVLIESALWNEINIAQSGRKLGINTDARYR
CEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEECCCCCCCCH
FERGVDPAFMLPGLELATKLVMEFCGGTPSEAVVVGNPFADDKIIDFPLAEVKRLAGIEV
HHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCHHHHHHHCCCEE
SLTEIRRILNHLGFTVVGQAPVVKVAVPSWRSDVHGKADIVEEIVRIVGVDKVPLTPFER
EHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GDAPRKPVLTQIQNRTRRAKRALAARGLTEAVTWSFISKPFAEAFGGGQPELALANPIAS
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHH
DLSDMRPSLLPGLIAAAQANADRGSPDLSLFEVGQVFKGDRPQDQFMAASGVRRGVASSA
HHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
GLGRHWSGSAQATALDAKADAFAVLAAAGAPMAGLQIATNKLPAWLHPGRSGAIQIGPQN
CCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCEEEECCCCCCEECCCCCCEEEECHHH
VLGYFGELHPRVLEQLGADGPLVAFEVILEKIPDPKQRPTRAKPALELSAFHPVSRDFAF
HHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCEEE
IVDRKVAVADIVRAAQGVDKKLITSVSVFDVYEGKGIDPDKKSVAIAVTLQPRDKTMTDQ
EECCHHHHHHHHHHHHCCCHHHHHHEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHH
EIEAVAAKIVAEVTKKTGGSLRG
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA