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The map label for this gene is fixI [H]

Identifier: 192288447

GI number: 192288447

Start: 14232

End: 16430

Strand: Reverse

Name: fixI [H]

Synonym: Rpal_0014

Alternate gene names: 192288447

Gene position: 16430-14232 (Counterclockwise)

Preceding gene: 192288448

Following gene: 192288446

Centisome position: 0.29

GC content: 67.44

Gene sequence:

>2199_bases
ATGCTGCAGACGCGGGATTTCTCTCACTACGTCAAAGACGTCGACGGCCGGATCGCGCATCTCGACCTCGCGGTGGAAGG
CGTTCACTGCGCCAGCTGCATGGCCAAGATCGAGCGCGGCCTGCAATCGATTCCGGATGTGACGCTCGCCCGCGTCAATC
TCACCGATCGTCGCCTTGCGGTGGAGTGGAACAAGGGCAGCACGCTGGAGCCCGGCCAGTTCATCGATCGCCTCGCCGAG
CTCGGCTACAAGGCCTATCCGTATGAGCCGGTGCGGGCCGAGGTCGAAGAGGCCGAACGCACCAAGTTCCTGCTCCGCTG
CCTCGGCGTCGCCGTGTTCGCCACGATGAACGTGATGATGCTGTCGGTGCCGGTGTGGTACGGCGGCGATCTCAGTCCCG
AGGCTCGCGACTTCTTCCATTGGTTGTCGGCGCTGGTCGCTCTGCCCTGTGCCGCCTATGCGGGGCAGCCGTTCTTCCAG
TCGGCGTTCCGCGCGCTGAAGGCCAAGAGCGTGAACATGGATGTGCCGATCACGATCGGCATCACGCTGGCGCTCGGCAT
GTCGCTGGTCGAGACCATCAATCACGCCGAGCACGCTTACTTCGACGCCGCTGTGATGCTGATCGCGTTCCTGCTGGCGG
GCCGCTTCCTCGATCAGAAGATGCGGCAGAAGACCCGCGCGGTTGCGGGTAACCTCGCAGCACTGAAGGCCGAGACCGCC
ACCAAGTTCGTCGGGCCCGACGAAATCAGCGAAGTGCCGATCGCCGCAGTGCGTGCCGGCGACATCGTGCTGCTGCGCCC
CGGCGAACGCTGCGCGGTCGACGGCACGGTGATCGAGGGCCGCTCCGAGATCGACCAGAGCCTGATCACCGGCGAGACGC
TGTATGTGCCGGCCGAACGCGGCACCCAGGTTTATGCCGGCACGCTCAACATCTCCGGTACCCTGCGCGTCCGCGTCGCT
GCGGCCGCAGAGGGCACGCTGCTGTCCGAGATCACCCGGCTGCTCGACCACGCGCTGCAGGCGCGTTCGCGCTACGTCCG
GCTCGCGGACCGCGCCTCCCAGCTCTATGCGCCGGTGGTGCACGCGACCGCGCTGCTGACGATCATCGGCTGGGTCCTGG
CCGGTGCGAGCTGGCACGATGCGATCGTGATCGGCATTGCGGTTCTGATCATCACCTGTCCCTGCGCGCTCGGCCTCGCG
ATTCCTGCGGTGCAGACCGTCGCCTCCGGCGCGATGTTCCGCGCCGGCGTGCTGCTCAATGCGGGCGACGCGATCGAGCG
CGCCGCTTCGGTCGACACCATCGTGTTCGACAAGACCGGTACGCTGACGCTGCCCGAGCTCGACGTCGTCAACGCCGCCG
GCATCCCGCTCGAGGTGTTCGACCTCGCCGGCCGGCTCGCCCTCGCCAGCCATCATCCGGTCGCCGCTGCGGTGGCGCGT
GCCGCGCACGCCAAGTCGCCGCTGCCGAATGTGGTCGAAGAACCGGGCCGCGGCGTTCGGACGGTGTTCGGCGGCCAAGA
ATTACGCCTCGGCAGCCCGGCGTTCTGCGGCGCCGATGCCGAAGCCAATCAGATCCTCAGCGAAGACCCCGAAGTGTCCG
TGGTGGCGTTCAGCCGCGGCGAGCAGAGGGTGGTATTCGCGGTCCGCCAGTTGCTGCGGCCCGACGCCGCCAAGACGATC
GACGCCCTGACCAATCGCGGCATTGCGGTCGAGCTCTTGTCCGGCGATCGCGAGCCGGCGGTTCGCCACGCGGCGGCTGC
GCTTGGTGTATCCGCCTGGCGCGCCAGCGTGACGCCGGCCGAGAAGATTGCTCACATCGAGGCGCTGAAGAAGCAGGGCC
GCATCGTGATGATGGTCGGCGACGGTATGAACGACGCACCGTCGCTCGCCGCGGCGCATGTCTCGATGTCGCCGATCAGC
GCCACGCATCTCAGCCAGGCCACCGCCGATCTGGTGTTCCTCGGCAAGGAGCTTGCGCCGGTGCTGGCCGCGGTCGATTA
TTCGCGGAAAGCGATGGCGGTGATGCGGGAGAATCTCGGTCTTGCCATCGGCTATAACGTACTGGCGGTGCCGATCGCGA
TCGCCGGCTATGCCACACCGCTGATCGCGGCGCTGGCGATGTCCGGATCGTCGCTGCTGGTGATGCTGAACGCGATGCGG
GCGCGCCGCGTCCGCCGGGGAGCCGCGTCGTGGAAGTAA

Upstream 100 bases:

>100_bases
GTTCTGGAAGGGGATCAGGGCGGCGTTCGCATGTTCCTCTCCAAGAACAAGCTGCTGTTGAACTAAGCGCCGATCCGGCA
GGCGTGACGAGGATCGCCGA

Downstream 100 bases:

>100_bases
TGGTGATCCTCGTCCCGCTGGCGCTCGGTCTCGGCCTGCTGGGATTGGTCGCGTTTCTGTGGTCGCTGAAGAGCGGCCAG
TATGAAGATCTCGACGGAGC

Product: ATPase P

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MLQTRDFSHYVKDVDGRIAHLDLAVEGVHCASCMAKIERGLQSIPDVTLARVNLTDRRLAVEWNKGSTLEPGQFIDRLAE
LGYKAYPYEPVRAEVEEAERTKFLLRCLGVAVFATMNVMMLSVPVWYGGDLSPEARDFFHWLSALVALPCAAYAGQPFFQ
SAFRALKAKSVNMDVPITIGITLALGMSLVETINHAEHAYFDAAVMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETA
TKFVGPDEISEVPIAAVRAGDIVLLRPGERCAVDGTVIEGRSEIDQSLITGETLYVPAERGTQVYAGTLNISGTLRVRVA
AAAEGTLLSEITRLLDHALQARSRYVRLADRASQLYAPVVHATALLTIIGWVLAGASWHDAIVIGIAVLIITCPCALGLA
IPAVQTVASGAMFRAGVLLNAGDAIERAASVDTIVFDKTGTLTLPELDVVNAAGIPLEVFDLAGRLALASHHPVAAAVAR
AAHAKSPLPNVVEEPGRGVRTVFGGQELRLGSPAFCGADAEANQILSEDPEVSVVAFSRGEQRVVFAVRQLLRPDAAKTI
DALTNRGIAVELLSGDREPAVRHAAAALGVSAWRASVTPAEKIAHIEALKKQGRIVMMVGDGMNDAPSLAAAHVSMSPIS
ATHLSQATADLVFLGKELAPVLAAVDYSRKAMAVMRENLGLAIGYNVLAVPIAIAGYATPLIAALAMSGSSLLVMLNAMR
ARRVRRGAASWK

Sequences:

>Translated_732_residues
MLQTRDFSHYVKDVDGRIAHLDLAVEGVHCASCMAKIERGLQSIPDVTLARVNLTDRRLAVEWNKGSTLEPGQFIDRLAE
LGYKAYPYEPVRAEVEEAERTKFLLRCLGVAVFATMNVMMLSVPVWYGGDLSPEARDFFHWLSALVALPCAAYAGQPFFQ
SAFRALKAKSVNMDVPITIGITLALGMSLVETINHAEHAYFDAAVMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETA
TKFVGPDEISEVPIAAVRAGDIVLLRPGERCAVDGTVIEGRSEIDQSLITGETLYVPAERGTQVYAGTLNISGTLRVRVA
AAAEGTLLSEITRLLDHALQARSRYVRLADRASQLYAPVVHATALLTIIGWVLAGASWHDAIVIGIAVLIITCPCALGLA
IPAVQTVASGAMFRAGVLLNAGDAIERAASVDTIVFDKTGTLTLPELDVVNAAGIPLEVFDLAGRLALASHHPVAAAVAR
AAHAKSPLPNVVEEPGRGVRTVFGGQELRLGSPAFCGADAEANQILSEDPEVSVVAFSRGEQRVVFAVRQLLRPDAAKTI
DALTNRGIAVELLSGDREPAVRHAAAALGVSAWRASVTPAEKIAHIEALKKQGRIVMMVGDGMNDAPSLAAAHVSMSPIS
ATHLSQATADLVFLGKELAPVLAAVDYSRKAMAVMRENLGLAIGYNVLAVPIAIAGYATPLIAALAMSGSSLLVMLNAMR
ARRVRRGAASWK
>Mature_732_residues
MLQTRDFSHYVKDVDGRIAHLDLAVEGVHCASCMAKIERGLQSIPDVTLARVNLTDRRLAVEWNKGSTLEPGQFIDRLAE
LGYKAYPYEPVRAEVEEAERTKFLLRCLGVAVFATMNVMMLSVPVWYGGDLSPEARDFFHWLSALVALPCAAYAGQPFFQ
SAFRALKAKSVNMDVPITIGITLALGMSLVETINHAEHAYFDAAVMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETA
TKFVGPDEISEVPIAAVRAGDIVLLRPGERCAVDGTVIEGRSEIDQSLITGETLYVPAERGTQVYAGTLNISGTLRVRVA
AAAEGTLLSEITRLLDHALQARSRYVRLADRASQLYAPVVHATALLTIIGWVLAGASWHDAIVIGIAVLIITCPCALGLA
IPAVQTVASGAMFRAGVLLNAGDAIERAASVDTIVFDKTGTLTLPELDVVNAAGIPLEVFDLAGRLALASHHPVAAAVAR
AAHAKSPLPNVVEEPGRGVRTVFGGQELRLGSPAFCGADAEANQILSEDPEVSVVAFSRGEQRVVFAVRQLLRPDAAKTI
DALTNRGIAVELLSGDREPAVRHAAAALGVSAWRASVTPAEKIAHIEALKKQGRIVMMVGDGMNDAPSLAAAHVSMSPIS
ATHLSQATADLVFLGKELAPVLAAVDYSRKAMAVMRENLGLAIGYNVLAVPIAIAGYATPLIAALAMSGSSLLVMLNAMR
ARRVRRGAASWK

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=799, Percent_Identity=25.531914893617, Blast_Score=225, Evalue=1e-58,
Organism=Homo sapiens, GI115529486, Length=781, Percent_Identity=26.5044814340589, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI55743073, Length=289, Percent_Identity=31.1418685121107, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI1786691, Length=748, Percent_Identity=31.0160427807487, Blast_Score=260, Evalue=3e-70,
Organism=Escherichia coli, GI1789879, Length=713, Percent_Identity=28.4712482468443, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1786914, Length=517, Percent_Identity=26.4990328820116, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17556548, Length=721, Percent_Identity=26.4909847434119, Blast_Score=162, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319772, Length=625, Percent_Identity=30.08, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6320475, Length=621, Percent_Identity=27.3752012882448, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321430, Length=511, Percent_Identity=24.26614481409, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325221, Length=550, Percent_Identity=23.8181818181818, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI221329854, Length=555, Percent_Identity=29.7297297297297, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 77832; Mature: 77832

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTRDFSHYVKDVDGRIAHLDLAVEGVHCASCMAKIERGLQSIPDVTLARVNLTDRRLA
CCCCCCHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEE
VEWNKGSTLEPGQFIDRLAELGYKAYPYEPVRAEVEEAERTKFLLRCLGVAVFATMNVMM
EEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVPVWYGGDLSPEARDFFHWLSALVALPCAAYAGQPFFQSAFRALKAKSVNMDVPITIG
HEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEH
ITLALGMSLVETINHAEHAYFDAAVMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TKFVGPDEISEVPIAAVRAGDIVLLRPGERCAVDGTVIEGRSEIDQSLITGETLYVPAER
HHCCCCCHHCCCCEEEEECCCEEEECCCCCEEECCEEECCHHHHHHHHHCCCEEEEECCC
GTQVYAGTLNISGTLRVRVAAAAEGTLLSEITRLLDHALQARSRYVRLADRASQLYAPVV
CCEEEEEEEEECEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HATALLTIIGWVLAGASWHDAIVIGIAVLIITCPCALGLAIPAVQTVASGAMFRAGVLLN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEE
AGDAIERAASVDTIVFDKTGTLTLPELDVVNAAGIPLEVFDLAGRLALASHHPVAAAVAR
CCHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCHHHHHHHCHHEECCCCCHHHHHHH
AAHAKSPLPNVVEEPGRGVRTVFGGQELRLGSPAFCGADAEANQILSEDPEVSVVAFSRG
HHHCCCCCCHHHHCCCCCCEEEECCCEEECCCCCCCCCCCHHHHHHCCCCCEEEEEECCC
EQRVVFAVRQLLRPDAAKTIDALTNRGIAVELLSGDREPAVRHAAAALGVSAWRASVTPA
CHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHH
EKIAHIEALKKQGRIVMMVGDGMNDAPSLAAAHVSMSPISATHLSQATADLVFLGKELAP
HHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
VLAAVDYSRKAMAVMRENLGLAIGYNVLAVPIAIAGYATPLIAALAMSGSSLLVMLNAMR
HHHHHCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ARRVRRGAASWK
HHHHHHCCCCCC
>Mature Secondary Structure
MLQTRDFSHYVKDVDGRIAHLDLAVEGVHCASCMAKIERGLQSIPDVTLARVNLTDRRLA
CCCCCCHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEE
VEWNKGSTLEPGQFIDRLAELGYKAYPYEPVRAEVEEAERTKFLLRCLGVAVFATMNVMM
EEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVPVWYGGDLSPEARDFFHWLSALVALPCAAYAGQPFFQSAFRALKAKSVNMDVPITIG
HEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEH
ITLALGMSLVETINHAEHAYFDAAVMLIAFLLAGRFLDQKMRQKTRAVAGNLAALKAETA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TKFVGPDEISEVPIAAVRAGDIVLLRPGERCAVDGTVIEGRSEIDQSLITGETLYVPAER
HHCCCCCHHCCCCEEEEECCCEEEECCCCCEEECCEEECCHHHHHHHHHCCCEEEEECCC
GTQVYAGTLNISGTLRVRVAAAAEGTLLSEITRLLDHALQARSRYVRLADRASQLYAPVV
CCEEEEEEEEECEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HATALLTIIGWVLAGASWHDAIVIGIAVLIITCPCALGLAIPAVQTVASGAMFRAGVLLN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEE
AGDAIERAASVDTIVFDKTGTLTLPELDVVNAAGIPLEVFDLAGRLALASHHPVAAAVAR
CCHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCHHHHHHHCHHEECCCCCHHHHHHH
AAHAKSPLPNVVEEPGRGVRTVFGGQELRLGSPAFCGADAEANQILSEDPEVSVVAFSRG
HHHCCCCCCHHHHCCCCCCEEEECCCEEECCCCCCCCCCCHHHHHHCCCCCEEEEEECCC
EQRVVFAVRQLLRPDAAKTIDALTNRGIAVELLSGDREPAVRHAAAALGVSAWRASVTPA
CHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHH
EKIAHIEALKKQGRIVMMVGDGMNDAPSLAAAHVSMSPISATHLSQATADLVFLGKELAP
HHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
VLAAVDYSRKAMAVMRENLGLAIGYNVLAVPIAIAGYATPLIAALAMSGSSLLVMLNAMR
HHHHHCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ARRVRRGAASWK
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661920; 12597275 [H]