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The map label for this gene is afr [H]

Identifier: 190895307

GI number: 190895307

Start: 1079570

End: 1080616

Strand: Reverse

Name: afr [H]

Synonym: RHECIAT_PC0000980

Alternate gene names: 190895307

Gene position: 1080616-1079570 (Counterclockwise)

Preceding gene: NA

Following gene: 190895305

Centisome position: 99.0

GC content: 63.99

Gene sequence:

>1047_bases
ATGCGTCTACTCATTCTCGGCACCGGCGGCATGGCCAATCAGCATGCCGCACGCTTCAAGGCGATCGAAGGCGTCAGCGT
CGTCGGCGGCGTCGATGTCGTTCCGGAGCGGCTCGCGGCCTTCTGCACCGAACACGGCATCGCCAATCATTTCCTCTCGC
TCGAGGATGCGCTTGCCTGGGGCGAGTTCGATGCGATCGCCAATGTCACGCCCGACAGCATCCACCACCCGACGACGCTG
CAGGCGATCGCCGCCGGCAAGCATGTCTTCTGCGAAAAGCCGCTCGCCACCGATGCGGCAAAAGCCATGGAGATGACCGA
GGCGATCGAGCGGGCGGGCAAGGTCGGCATGGTCAATTTCACCTACCGCAACTCGCCGGCGCTGCAGAAGGGCCGGCAGA
TGGTGCTTGCCGGCGAGATTGGCGCGGTGCGCCATGTCGAGGCGTCGCATCTGCAGAGCTGGCTGGTCGGCCGGCACTGG
GGCGATTGGAAGAGCGAGAGCAAATGGCTGTGGCGGCTGAGCAAGAAGCACGGCTCGAACGGCGCGCTCGGCGATATCGG
CATCCATATCGTCGATTTCGCCTCCTACGGCTCCGGCCTCGACGTCGCCCATGTCTTCGCCCGGCTGAAGACCTTCGACA
AGGCGCCCGGCCACAAGATCGGCGAGTATGATCTCGATGCCAATGACAGTTTCACCATGAATGTCGATTTCACCAATGGC
GCGATCGGCACGATCCAGGCGACGCGCACCGCCGCCGGCCAGATGGACCAGCTGCGGCTCAGGGTTTACGGCGAAACCGG
CTCGGTCGAGATGATCTACGATACCGGCAAATCGACGCTGCGCGCCTGCCTCGGCGAAGACGTCCACACGGCGACCTGGC
GCGACGTGCCCTTCGATCCCGTCGTGACCAATTACCAGCGTTTCGTCCAGGCGGTGCGGGCGGGCAAAACCCAGGAGCCG
TCTTTCCGCCGCGCGGCGAACATCCAGAAGGTGCTCGACAAGGCACTCGCCTCCGACCTCAGTCACAAAGACGAGGCGCT
CACTTAA

Upstream 100 bases:

>100_bases
TTCCTGAAGAAGGCCATGAGGCAATCGCGATGGTCGCGATGGCCCGGTCTGGTTTTTGCCCGGCTGCGGCGCAGCCGGAA
GTCAGCCCACGGAGGATAAG

Downstream 100 bases:

>100_bases
GCCGATGCCGGCGGCAGCAGCAGCGTGTTGAGGAAGGGCAGCAGCGCCGCGCCGAGCGCTACGCCGCCGCCGCTGAAGCT
GGCGGGCCGCATCGGCGAAA

Product: putative oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MRLLILGTGGMANQHAARFKAIEGVSVVGGVDVVPERLAAFCTEHGIANHFLSLEDALAWGEFDAIANVTPDSIHHPTTL
QAIAAGKHVFCEKPLATDAAKAMEMTEAIERAGKVGMVNFTYRNSPALQKGRQMVLAGEIGAVRHVEASHLQSWLVGRHW
GDWKSESKWLWRLSKKHGSNGALGDIGIHIVDFASYGSGLDVAHVFARLKTFDKAPGHKIGEYDLDANDSFTMNVDFTNG
AIGTIQATRTAAGQMDQLRLRVYGETGSVEMIYDTGKSTLRACLGEDVHTATWRDVPFDPVVTNYQRFVQAVRAGKTQEP
SFRRAANIQKVLDKALASDLSHKDEALT

Sequences:

>Translated_348_residues
MRLLILGTGGMANQHAARFKAIEGVSVVGGVDVVPERLAAFCTEHGIANHFLSLEDALAWGEFDAIANVTPDSIHHPTTL
QAIAAGKHVFCEKPLATDAAKAMEMTEAIERAGKVGMVNFTYRNSPALQKGRQMVLAGEIGAVRHVEASHLQSWLVGRHW
GDWKSESKWLWRLSKKHGSNGALGDIGIHIVDFASYGSGLDVAHVFARLKTFDKAPGHKIGEYDLDANDSFTMNVDFTNG
AIGTIQATRTAAGQMDQLRLRVYGETGSVEMIYDTGKSTLRACLGEDVHTATWRDVPFDPVVTNYQRFVQAVRAGKTQEP
SFRRAANIQKVLDKALASDLSHKDEALT
>Mature_348_residues
MRLLILGTGGMANQHAARFKAIEGVSVVGGVDVVPERLAAFCTEHGIANHFLSLEDALAWGEFDAIANVTPDSIHHPTTL
QAIAAGKHVFCEKPLATDAAKAMEMTEAIERAGKVGMVNFTYRNSPALQKGRQMVLAGEIGAVRHVEASHLQSWLVGRHW
GDWKSESKWLWRLSKKHGSNGALGDIGIHIVDFASYGSGLDVAHVFARLKTFDKAPGHKIGEYDLDANDSFTMNVDFTNG
AIGTIQATRTAAGQMDQLRLRVYGETGSVEMIYDTGKSTLRACLGEDVHTATWRDVPFDPVVTNYQRFVQAVRAGKTQEP
SFRRAANIQKVLDKALASDLSHKDEALT

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=251, Percent_Identity=29.0836653386454, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 37922; Mature: 37922

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLILGTGGMANQHAARFKAIEGVSVVGGVDVVPERLAAFCTEHGIANHFLSLEDALAW
CEEEEEECCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GEFDAIANVTPDSIHHPTTLQAIAAGKHVFCEKPLATDAAKAMEMTEAIERAGKVGMVNF
CCHHHHCCCCCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEE
TYRNSPALQKGRQMVLAGEIGAVRHVEASHLQSWLVGRHWGDWKSESKWLWRLSKKHGSN
EECCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
GALGDIGIHIVDFASYGSGLDVAHVFARLKTFDKAPGHKIGEYDLDANDSFTMNVDFTNG
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCC
AIGTIQATRTAAGQMDQLRLRVYGETGSVEMIYDTGKSTLRACLGEDVHTATWRDVPFDP
CEEEEEEHHHHHCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCH
VVTNYQRFVQAVRAGKTQEPSFRRAANIQKVLDKALASDLSHKDEALT
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLLILGTGGMANQHAARFKAIEGVSVVGGVDVVPERLAAFCTEHGIANHFLSLEDALAW
CEEEEEECCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GEFDAIANVTPDSIHHPTTLQAIAAGKHVFCEKPLATDAAKAMEMTEAIERAGKVGMVNF
CCHHHHCCCCCCCCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEE
TYRNSPALQKGRQMVLAGEIGAVRHVEASHLQSWLVGRHWGDWKSESKWLWRLSKKHGSN
EECCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
GALGDIGIHIVDFASYGSGLDVAHVFARLKTFDKAPGHKIGEYDLDANDSFTMNVDFTNG
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCC
AIGTIQATRTAAGQMDQLRLRVYGETGSVEMIYDTGKSTLRACLGEDVHTATWRDVPFDP
CEEEEEEHHHHHCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCH
VVTNYQRFVQAVRAGKTQEPSFRRAANIQKVLDKALASDLSHKDEALT
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA