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The map label for this gene is hyuA [H]

Identifier: 190894953

GI number: 190894953

Start: 670341

End: 672404

Strand: Reverse

Name: hyuA [H]

Synonym: RHECIAT_PC0000618

Alternate gene names: 190894953

Gene position: 672404-670341 (Counterclockwise)

Preceding gene: 190894954

Following gene: 190894952

Centisome position: 61.6

GC content: 63.95

Gene sequence:

>2064_bases
ATGGCAAAACTGGCTTTCGACACTGGCGGCACCTTCACCGACTTCGCATTGCTTGATGACAATGGCGATCTCCATCTGCA
CAAGGTGCTGAGCACGCCGAAGAACCCGGCGGAAGCGGTCGTGCAGGGCGTGTCGGAACTCCTGGAACAGTTCTCCTCCG
CCATCTCGATCGAACGTCTGCAGGTCTTGGGTGCGACGACGGTGGTGACGAATGCTGTGCTGGAGCGCAAGGGCGTCGAG
ACCAGCTTCATCACCACCGATGGCTTTCAGGATATGCTTCGCATCCGCAACGAAGGCCGCTACGACCTCTACGATCTCAA
CATCAAATATCCGGAGCCGCTGGTCACTCGGTCGAACAGTTATGGCGCCGTCGAGCGCATCGCGGCCGATGGCGAGGTGA
TGACCGAACTCAAGGAAGAGACCGTCCGTGAAATTGCGTCGCATCTGAAGAAGAAGGGCATCCGTTCCGTCGCCGTGTGC
CTGCTGCACGCCTATAAATATCCGCAGCACGAACAGCGTGTGGCAGCCCTCCTGCGCGAGGAAGACCCCGATATTTTCGT
CTCGTTGTCGTCGGAGGTCTGCCCTGAGATGCGGGAATTCGACCGCGCCTCGACGACGGTCGTCAATGCCTATACCCGCC
CGCAGATGGCCGGTCACGTCGCTCATCTTCAGCGCGAATTCGCCACCAAAGGCATCGACCGCCAGGTTCTCTGGATGACC
TCGTCGGGCGGCCTGGTGCCGAGCCGGCGCGCCGCCGAACTTCCCGTCCGCCTGATCGAATCCGGTCCCGCAGCTGGCGC
CGTCGCCGCCGCCGAATTCGGCCGCATTGCCGGCGAGGGAAGCGTGCTTTCCTTCGACATGGGCGGCACCACCGCGAAGC
TTTGCCTGATCCCGAACGGCGAGCCGACGGTCGGAACGGACCTTGAGGTCGCTCACTATCAGCGTTTCCGCAAGGGCTCC
GGCTTCCCGCTGAAGATCCAGTCCATCCAGATGATCGAAATCGGCGCCGGCGGCGGGTCGATCGCGGCAAAGAACCCGCT
GGGTCTTCTCGATGTCGGCCCGCATTCGGCGGGCGCGATGCCGGGTCCGGCGGCCTATCAGCGCGGCGGCACGCAGCCGA
CGGTCACCGATGCGGATATCCTGCTTGGCTATATGGGGACGGAGTCCTTCGTCGGTGGTTCCTTCAAAGTGTCGAAGGAC
GCGGCGCGCGAAGCGATGGCCGCACTTGCCAAGTCGCTCGATGTCAGCGTCGACCGCTGCGCCTGGGGCATTCACGATCT
CGTCAATGAATCCATGAGCAAGGCGGCGGCCATGCATGCCACCGATCTCGGTGTCGATCCGCGGTCGCTGCCGATGGTGG
CATTCGGCGGCGCGGGCCCGGTGCATGCCTACGGCATCGCCTGCAAGCTCGGCATCAAGCGCATCATCTGCCCGAGGGGG
GCAGGCGTCACCTCGGCCATCGGCCTGCTGATCGCGCCGGTCGCCGTCGATCTCTCCGCAAGTCATCCGATGCCGGTCGA
CAATTGGGATGTCGCCGCGGTCGACCGTCTGCTGGACGATCTGGCTGCGCAGGGGGCGGAGGTGGTCTCCGCCGCCGGCG
TCGCCAAGGAGACGATCACCAACCGCTACACGGTCGACATGCGCCATGTTGGCCAGGGGCACGAGATCACCGTGGCTCTG
CCCGATCGCGGCCTGCCGAAGGAGGAGTTCCTCAAGCAGCTGCTCGCCAATTTCTACAAGCTCTATCGCGAGCTGTTCGG
CCGGACGGTCAACGCGTCGGTCGAGGTCATCACCTGGCGGCTTCGCGCCAGCGGCGAAAAGGATCGGGTGACCCGGCCTC
ACGAAGTCAATGCCGCCGATGCGTCGAAGGGGCAGCGCCAGGTCTATTTCCAGGAGCTCGGCGCCTATGCCGAGACGCCT
GTCTACGACCACTACCGGCTTCCGGTGGGGCGCGAATTCGCTGGGCCGGCGATTGTCGAGCAGCGCGAATCCACCGCCGT
CGTCGGGCCAAGTGGCGTCTTCCACGTCGATGCCAACGGCAACCTCGTGATCAACATTCAGTAA

Upstream 100 bases:

>100_bases
GCAACTGTGTTAGCCAAGCCGGTCGCCGATCACCTAACCTCCTCAGCAGACATAAGGGTCTCCGAAACCGCATGAGACCA
ACGGTTGCTTCGAGGGAAAC

Downstream 100 bases:

>100_bases
GGCGAATTCCGATGTCGAAGACCGCTACCGATTTCAACGATCCGATCAATCTGCAGGTCATGTGGAACCGCTTGATCTTC
ATCGCCGATCAGGCCGACAA

Product: hydantoin utilization N-methylhydantoinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MAKLAFDTGGTFTDFALLDDNGDLHLHKVLSTPKNPAEAVVQGVSELLEQFSSAISIERLQVLGATTVVTNAVLERKGVE
TSFITTDGFQDMLRIRNEGRYDLYDLNIKYPEPLVTRSNSYGAVERIAADGEVMTELKEETVREIASHLKKKGIRSVAVC
LLHAYKYPQHEQRVAALLREEDPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFATKGIDRQVLWMT
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRIAGEGSVLSFDMGGTTAKLCLIPNGEPTVGTDLEVAHYQRFRKGS
GFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAMPGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKD
AAREAMAALAKSLDVSVDRCAWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIACKLGIKRIICPRG
AGVTSAIGLLIAPVAVDLSASHPMPVDNWDVAAVDRLLDDLAAQGAEVVSAAGVAKETITNRYTVDMRHVGQGHEITVAL
PDRGLPKEEFLKQLLANFYKLYRELFGRTVNASVEVITWRLRASGEKDRVTRPHEVNAADASKGQRQVYFQELGAYAETP
VYDHYRLPVGREFAGPAIVEQRESTAVVGPSGVFHVDANGNLVINIQ

Sequences:

>Translated_687_residues
MAKLAFDTGGTFTDFALLDDNGDLHLHKVLSTPKNPAEAVVQGVSELLEQFSSAISIERLQVLGATTVVTNAVLERKGVE
TSFITTDGFQDMLRIRNEGRYDLYDLNIKYPEPLVTRSNSYGAVERIAADGEVMTELKEETVREIASHLKKKGIRSVAVC
LLHAYKYPQHEQRVAALLREEDPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFATKGIDRQVLWMT
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRIAGEGSVLSFDMGGTTAKLCLIPNGEPTVGTDLEVAHYQRFRKGS
GFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAMPGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKD
AAREAMAALAKSLDVSVDRCAWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIACKLGIKRIICPRG
AGVTSAIGLLIAPVAVDLSASHPMPVDNWDVAAVDRLLDDLAAQGAEVVSAAGVAKETITNRYTVDMRHVGQGHEITVAL
PDRGLPKEEFLKQLLANFYKLYRELFGRTVNASVEVITWRLRASGEKDRVTRPHEVNAADASKGQRQVYFQELGAYAETP
VYDHYRLPVGREFAGPAIVEQRESTAVVGPSGVFHVDANGNLVINIQ
>Mature_686_residues
AKLAFDTGGTFTDFALLDDNGDLHLHKVLSTPKNPAEAVVQGVSELLEQFSSAISIERLQVLGATTVVTNAVLERKGVET
SFITTDGFQDMLRIRNEGRYDLYDLNIKYPEPLVTRSNSYGAVERIAADGEVMTELKEETVREIASHLKKKGIRSVAVCL
LHAYKYPQHEQRVAALLREEDPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFATKGIDRQVLWMTS
SGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRIAGEGSVLSFDMGGTTAKLCLIPNGEPTVGTDLEVAHYQRFRKGSG
FPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAMPGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKDA
AREAMAALAKSLDVSVDRCAWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIACKLGIKRIICPRGA
GVTSAIGLLIAPVAVDLSASHPMPVDNWDVAAVDRLLDDLAAQGAEVVSAAGVAKETITNRYTVDMRHVGQGHEITVALP
DRGLPKEEFLKQLLANFYKLYRELFGRTVNASVEVITWRLRASGEKDRVTRPHEVNAADASKGQRQVYFQELGAYAETPV
YDHYRLPVGREFAGPAIVEQRESTAVVGPSGVFHVDANGNLVINIQ

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=743, Percent_Identity=30.4172274562584, Blast_Score=221, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI133901900, Length=727, Percent_Identity=26.4099037138927, Blast_Score=192, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322634, Length=737, Percent_Identity=27.9511533242877, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI45550492, Length=746, Percent_Identity=29.088471849866, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 73767; Mature: 73636

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLAFDTGGTFTDFALLDDNGDLHLHKVLSTPKNPAEAVVQGVSELLEQFSSAISIERL
CCEEEECCCCCEEEEEEEECCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVLGATTVVTNAVLERKGVETSFITTDGFQDMLRIRNEGRYDLYDLNIKYPEPLVTRSNS
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEECCCCCEECCCC
YGAVERIAADGEVMTELKEETVREIASHLKKKGIRSVAVCLLHAYKYPQHEQRVAALLRE
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
EDPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFATKGIDRQVLWMT
CCCCEEEEECHHHCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRIAGEGSVLSFDMGGTTAKLCLIPNG
CCCCCCCCCCHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCC
EPTVGTDLEVAHYQRFRKGSGFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAM
CCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCC
PGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKDAAREAMAALAKSLDVSVDRC
CCCHHHHCCCCCCCCCCHHHHEEECCCHHHCCCCEEECHHHHHHHHHHHHHHHCCCHHHH
AWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIACKLGIKRIICPRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCHHEECCCC
AGVTSAIGLLIAPVAVDLSASHPMPVDNWDVAAVDRLLDDLAAQGAEVVSAAGVAKETIT
CCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
NRYTVDMRHVGQGHEITVALPDRGLPKEEFLKQLLANFYKLYRELFGRTVNASVEVITWR
CCEEEEHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
LRASGEKDRVTRPHEVNAADASKGQRQVYFQELGAYAETPVYDHYRLPVGREFAGPAIVE
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCCEECCCCCCCCCCHHHH
QRESTAVVGPSGVFHVDANGNLVINIQ
CCCCCEEECCCCEEEECCCCCEEEEEC
>Mature Secondary Structure 
AKLAFDTGGTFTDFALLDDNGDLHLHKVLSTPKNPAEAVVQGVSELLEQFSSAISIERL
CEEEECCCCCEEEEEEEECCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVLGATTVVTNAVLERKGVETSFITTDGFQDMLRIRNEGRYDLYDLNIKYPEPLVTRSNS
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEECCCCCEECCCC
YGAVERIAADGEVMTELKEETVREIASHLKKKGIRSVAVCLLHAYKYPQHEQRVAALLRE
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
EDPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFATKGIDRQVLWMT
CCCCEEEEECHHHCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRIAGEGSVLSFDMGGTTAKLCLIPNG
CCCCCCCCCCHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCC
EPTVGTDLEVAHYQRFRKGSGFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAM
CCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCC
PGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKDAAREAMAALAKSLDVSVDRC
CCCHHHHCCCCCCCCCCHHHHEEECCCHHHCCCCEEECHHHHHHHHHHHHHHHCCCHHHH
AWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIACKLGIKRIICPRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCHHEECCCC
AGVTSAIGLLIAPVAVDLSASHPMPVDNWDVAAVDRLLDDLAAQGAEVVSAAGVAKETIT
CCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
NRYTVDMRHVGQGHEITVALPDRGLPKEEFLKQLLANFYKLYRELFGRTVNASVEVITWR
CCEEEEHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
LRASGEKDRVTRPHEVNAADASKGQRQVYFQELGAYAETPVYDHYRLPVGREFAGPAIVE
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCCEECCCCCCCCCCHHHH
QRESTAVVGPSGVFHVDANGNLVINIQ
CCCCCEEECCCCEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]