Click here to switch to the map view.

The map label for this gene is hyuB [H]

Identifier: 190894952

GI number: 190894952

Start: 668653

End: 670329

Strand: Reverse

Name: hyuB [H]

Synonym: RHECIAT_PC0000617

Alternate gene names: 190894952

Gene position: 670329-668653 (Counterclockwise)

Preceding gene: 190894953

Following gene: 190894951

Centisome position: 61.41

GC content: 61.54

Gene sequence:

>1677_bases
ATGTCGAAGACCGCTACCGATTTCAACGATCCGATCAATCTGCAGGTCATGTGGAACCGCTTGATCTTCATCGCCGATCA
GGCCGACAACGTGCTCGGCAAGACGGCCTTCTCGCCGATCGTCCGTGAGAACCACGATTATGTCACCGTGCTGCTCGACA
GCAAGGGGCGGGCGCTTGCCCAATGCACCTGGTCCATCCCGGTCTTCATCACCTCGCTGCCGGCCGCGGCTCAGAAATAT
TTCCTGCCGAAGTTTCCGGCGGAAACGCTTGTCGAAGGCGATGTTCTCGCGACCAACGATCCGGAGATCGGCACCGGCCA
TCTGCCCGATGTGACGATGATCACGCCGATCTTCAAGAACGGCAAGGTCGTCGCCTATGCCGGCTCGATCGCTCACCTGC
CTGACATCGGCGGAGCGCCGCTTCATTCGGAAGCGAGCGACATTTTCGAGGAAGGCATCCGTTTCCCGATCGTCAAACTG
CACAAAGCCGGCGTTCCCAATCAGGACGTTCTCGACATCATCGCCGCATCGGTGCGGCTGCCGACCGAAGTCCAGGGCGA
TCTGGAATCGATGATCGCTGCCAACAATGTCATGGGTCGCGAACTGGTGAAATTCCTCGATGAATATGGCCTCGACGATA
TCGACGGCCTCGCCGAAGCGATCCATTCCCGCTCTGAGGCGCAGACCCGCAAGGCGATCAAGGCGTGGCCGAATGGCCGC
TATTCGGCCCAGATGACGCTGGACGGCTACGAGACCGACATCATCTTGAAGGCGACGGTCATCGTCGGCGACGATTCCAT
CCATGTGGATTACGCCGGCACATCCGATCAGGTTCTCCACTCGATCAACTGTCGCACCAACTATCGCTACGCCCATTCGG
TCTATGCGCTGAAATGCCTGCTCGATCCCGATACGCCGAACAACGAGGGCTGCATCACGCCGATCACCGACGACGCTCCG
CTTGGCTCCGTTCTCAATCCGGAGCAGTGGACGGCGGGCAATTCCCGCAACTTGATCGGCCATGCGATCCCCTCGCTGAT
CTTCCGGGCGCTCGAAGGCGTGGTGCCCGACAAGGTCATGGGCGACAGCGGCGGCGCGCCCATATGGGCGGCCAACTGCG
TCGGCCGCCGGGATGACGGTTCGCAGTACGGATCCGTGCAGAATTTCCATGGCGGCCAGGGGGCTCGTGCGGAATTCGAC
GGGCTGGATACGCTGAGCTTTCCATCGAACTGTAAGGTGACGGCCGTCGAAATGTTCGAAATCGCCGTGCCCGTGTTGAT
GGAGCGCAAGGAACTCATCGCCGATTCCGGCGGGGCCGGCAAATATCGCGGCGGCCTGGGACAGCGGGCAATCCTGCGCA
ATCTCGGCCGCTCGGCAATGAACATCTATCTCGCATCCGAGCGTGTCCGGCATCCCTGCTTCGGTGTCGTCGACGGACAG
GACGGCACGCCGGGCAAGGTCAGCAAGGAAGGCCAGCCGCAATTTCCAAAGGGCAAGGTCGTGCTGAAGACCGGCGAGCG
GCTGGAAGTCGAAACGCCGGGCGGCGGCGGATGGGGCAGGGTGAGCGAGCGGCCGGCCGTGCTGATCGAGCAGGATCTTG
CCGAAGGCCTGATCACCCCAAAAGCCGCAAAGCTTATATATGGCTACAAGGGCGCGGCCGCGGTGGCCGCGGAATAG

Upstream 100 bases:

>100_bases
TCGAGCAGCGCGAATCCACCGCCGTCGTCGGGCCAAGTGGCGTCTTCCACGTCGATGCCAACGGCAACCTCGTGATCAAC
ATTCAGTAAGGCGAATTCCG

Downstream 100 bases:

>100_bases
GAGGAGCCATTATGGGCCTGTTGAACGGAAAGATCGCGCTTGTCACCGGAGCGGGAACGGGCATCGGTCGCGAGGCCGCG
ATCCTTCTTGCCGAGGAAGG

Product: hydantoin utilization 5-oxoprolinase

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSKTATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALAQCTWSIPVFITSLPAAAQKY
FLPKFPAETLVEGDVLATNDPEIGTGHLPDVTMITPIFKNGKVVAYAGSIAHLPDIGGAPLHSEASDIFEEGIRFPIVKL
HKAGVPNQDVLDIIAASVRLPTEVQGDLESMIAANNVMGRELVKFLDEYGLDDIDGLAEAIHSRSEAQTRKAIKAWPNGR
YSAQMTLDGYETDIILKATVIVGDDSIHVDYAGTSDQVLHSINCRTNYRYAHSVYALKCLLDPDTPNNEGCITPITDDAP
LGSVLNPEQWTAGNSRNLIGHAIPSLIFRALEGVVPDKVMGDSGGAPIWAANCVGRRDDGSQYGSVQNFHGGQGARAEFD
GLDTLSFPSNCKVTAVEMFEIAVPVLMERKELIADSGGAGKYRGGLGQRAILRNLGRSAMNIYLASERVRHPCFGVVDGQ
DGTPGKVSKEGQPQFPKGKVVLKTGERLEVETPGGGGWGRVSERPAVLIEQDLAEGLITPKAAKLIYGYKGAAAVAAE

Sequences:

>Translated_558_residues
MSKTATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALAQCTWSIPVFITSLPAAAQKY
FLPKFPAETLVEGDVLATNDPEIGTGHLPDVTMITPIFKNGKVVAYAGSIAHLPDIGGAPLHSEASDIFEEGIRFPIVKL
HKAGVPNQDVLDIIAASVRLPTEVQGDLESMIAANNVMGRELVKFLDEYGLDDIDGLAEAIHSRSEAQTRKAIKAWPNGR
YSAQMTLDGYETDIILKATVIVGDDSIHVDYAGTSDQVLHSINCRTNYRYAHSVYALKCLLDPDTPNNEGCITPITDDAP
LGSVLNPEQWTAGNSRNLIGHAIPSLIFRALEGVVPDKVMGDSGGAPIWAANCVGRRDDGSQYGSVQNFHGGQGARAEFD
GLDTLSFPSNCKVTAVEMFEIAVPVLMERKELIADSGGAGKYRGGLGQRAILRNLGRSAMNIYLASERVRHPCFGVVDGQ
DGTPGKVSKEGQPQFPKGKVVLKTGERLEVETPGGGGWGRVSERPAVLIEQDLAEGLITPKAAKLIYGYKGAAAVAAE
>Mature_557_residues
SKTATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALAQCTWSIPVFITSLPAAAQKYF
LPKFPAETLVEGDVLATNDPEIGTGHLPDVTMITPIFKNGKVVAYAGSIAHLPDIGGAPLHSEASDIFEEGIRFPIVKLH
KAGVPNQDVLDIIAASVRLPTEVQGDLESMIAANNVMGRELVKFLDEYGLDDIDGLAEAIHSRSEAQTRKAIKAWPNGRY
SAQMTLDGYETDIILKATVIVGDDSIHVDYAGTSDQVLHSINCRTNYRYAHSVYALKCLLDPDTPNNEGCITPITDDAPL
GSVLNPEQWTAGNSRNLIGHAIPSLIFRALEGVVPDKVMGDSGGAPIWAANCVGRRDDGSQYGSVQNFHGGQGARAEFDG
LDTLSFPSNCKVTAVEMFEIAVPVLMERKELIADSGGAGKYRGGLGQRAILRNLGRSAMNIYLASERVRHPCFGVVDGQD
GTPGKVSKEGQPQFPKGKVVLKTGERLEVETPGGGGWGRVSERPAVLIEQDLAEGLITPKAAKLIYGYKGAAAVAAE

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=545, Percent_Identity=28.4403669724771, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI133901900, Length=556, Percent_Identity=28.2374100719424, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI133901902, Length=515, Percent_Identity=27.5728155339806, Blast_Score=142, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322634, Length=568, Percent_Identity=28.6971830985916, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45550492, Length=552, Percent_Identity=26.4492753623188, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: NA

Molecular weight: Translated: 59960; Mature: 59828

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALA
CCCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
QCTWSIPVFITSLPAAAQKYFLPKFPAETLVEGDVLATNDPEIGTGHLPDVTMITPIFKN
EEEEECEEEEECCCHHHHHHCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEECC
GKVVAYAGSIAHLPDIGGAPLHSEASDIFEEGIRFPIVKLHKAGVPNQDVLDIIAASVRL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PTEVQGDLESMIAANNVMGRELVKFLDEYGLDDIDGLAEAIHSRSEAQTRKAIKAWPNGR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
YSAQMTLDGYETDIILKATVIVGDDSIHVDYAGTSDQVLHSINCRTNYRYAHSVYALKCL
EEEEEEECCCCCEEEEEEEEEEECCEEEEEECCCHHHHHEEECCCCCCEEEHEEEEEEEE
LDPDTPNNEGCITPITDDAPLGSVLNPEQWTAGNSRNLIGHAIPSLIFRALEGVVPDKVM
ECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
GDSGGAPIWAANCVGRRDDGSQYGSVQNFHGGQGARAEFDGLDTLSFPSNCKVTAVEMFE
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHH
IAVPVLMERKELIADSGGAGKYRGGLGQRAILRNLGRSAMNIYLASERVRHPCFGVVDGQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHEEEEEEHHHHCCCEEEEEECC
DGTPGKVSKEGQPQFPKGKVVLKTGERLEVETPGGGGWGRVSERPAVLIEQDLAEGLITP
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCEEEHHHHHCCCCCC
KAAKLIYGYKGAAAVAAE
CHHHEEECCCCCEEECCC
>Mature Secondary Structure 
SKTATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALA
CCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
QCTWSIPVFITSLPAAAQKYFLPKFPAETLVEGDVLATNDPEIGTGHLPDVTMITPIFKN
EEEEECEEEEECCCHHHHHHCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEECC
GKVVAYAGSIAHLPDIGGAPLHSEASDIFEEGIRFPIVKLHKAGVPNQDVLDIIAASVRL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PTEVQGDLESMIAANNVMGRELVKFLDEYGLDDIDGLAEAIHSRSEAQTRKAIKAWPNGR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
YSAQMTLDGYETDIILKATVIVGDDSIHVDYAGTSDQVLHSINCRTNYRYAHSVYALKCL
EEEEEEECCCCCEEEEEEEEEEECCEEEEEECCCHHHHHEEECCCCCCEEEHEEEEEEEE
LDPDTPNNEGCITPITDDAPLGSVLNPEQWTAGNSRNLIGHAIPSLIFRALEGVVPDKVM
ECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
GDSGGAPIWAANCVGRRDDGSQYGSVQNFHGGQGARAEFDGLDTLSFPSNCKVTAVEMFE
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHH
IAVPVLMERKELIADSGGAGKYRGGLGQRAILRNLGRSAMNIYLASERVRHPCFGVVDGQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHEEEEEEHHHHCCCEEEEEECC
DGTPGKVSKEGQPQFPKGKVVLKTGERLEVETPGGGGWGRVSERPAVLIEQDLAEGLITP
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCEEEHHHHHCCCCCC
KAAKLIYGYKGAAAVAAE
CHHHEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]