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The map label for this gene is yneJ [H]

Identifier: 190894869

GI number: 190894869

Start: 581072

End: 581977

Strand: Reverse

Name: yneJ [H]

Synonym: RHECIAT_PC0000534

Alternate gene names: 190894869

Gene position: 581977-581072 (Counterclockwise)

Preceding gene: 190894871

Following gene: 190894868

Centisome position: 53.32

GC content: 63.13

Gene sequence:

>906_bases
TTGGACCTTTCGTCAATCGAAATATTCCTCGCCGTTGCCAGCGATTGCAGCGTGACGAAGGCGGCCAAGGCCGTCGGGCG
AGTGCCGTCGAACGTGACGACGCGCATCCAGCAATTGGAGGAGGATCTCGGCGCCTCCCTCTTCAGCCGTGATGGCAAGA
AGATGACGTTGACGCGGGAAGGCGAGACGTTTCTCACCTATGCCAACCGGCTTATGGCACTTGCGCTCGAAGCGCGCCAA
GCCGTGCGGCCGCTTGCCCCATCCGGAACATTGCGGGTCGGCACGATGGAGAGCACGGCGGCAAGCCGCCTGCCGGCGGC
GCTCACGCAGTTCAACCAGATGTGGCCTGAAGTGTCACTGCATCTGACGATGGGCGCGTCCCGTGATCTCGCCCGCGATG
TTCTGGCCGACGCGCTGGATTGCGCCTTGATCGCCCGGCCGCCGAAGACGATGCGCGAGGATGACCTGGAGTTCGATGCC
GAACTCAAGGCGCTGGAAAAAGAGACGGTTTTTGTCGAAGATCTGTTGATCGTGCTGCCATCAGGACATCCCGCCATCAA
ATCGGCCGCCGACCTGCGTGTCGGAGCGCTCGCCGCTTTGGAGCCTGGCTGCACCTATCGCAGGATCGCCGAAAACTGGG
CGCGCAAATCGACCGCCCTGCCGACGAGCGAGCTCGGCTCCTACCACGCGATCCTGGCGAGCGTTGCGACCGGCAACACC
GCGGGTGTGATGCCGAAATCCGTGCTCGAGCTCATGCACTGGCCGACCGCTGTCCAGACCCATCAGCTCGGCCCCGTCGA
GACGCTGCTGGTCTACCGTCGGGATAATCGCCCCAGCGCCTTCAGTGCCTTTCACGAGGTCCTCAGCGCTAGAAAGGGCA
GAGAGATCCGACTGGCGACAAACTAG

Upstream 100 bases:

>100_bases
CTCGATCGGCAAATTATGTCTTATTGCGACTTGAGGCATGGTGATGATTTCGATATGGAGAACTATCCCCAAGGTTAATT
CCGGAAACGAGATCTTTTCA

Downstream 100 bases:

>100_bases
TTTCTCCGTCTTCTCCTTCGCCCGCCAGACAATCCTCGCTTATAATTGGTCTTAGCATGCAATTTCCCGTTTCCCCGAAG
GTAGCGCCGAACCGTTTTCG

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDLSSIEIFLAVASDCSVTKAAKAVGRVPSNVTTRIQQLEEDLGASLFSRDGKKMTLTREGETFLTYANRLMALALEARQ
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPEVSLHLTMGASRDLARDVLADALDCALIARPPKTMREDDLEFDA
ELKALEKETVFVEDLLIVLPSGHPAIKSAADLRVGALAALEPGCTYRRIAENWARKSTALPTSELGSYHAILASVATGNT
AGVMPKSVLELMHWPTAVQTHQLGPVETLLVYRRDNRPSAFSAFHEVLSARKGREIRLATN

Sequences:

>Translated_301_residues
MDLSSIEIFLAVASDCSVTKAAKAVGRVPSNVTTRIQQLEEDLGASLFSRDGKKMTLTREGETFLTYANRLMALALEARQ
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPEVSLHLTMGASRDLARDVLADALDCALIARPPKTMREDDLEFDA
ELKALEKETVFVEDLLIVLPSGHPAIKSAADLRVGALAALEPGCTYRRIAENWARKSTALPTSELGSYHAILASVATGNT
AGVMPKSVLELMHWPTAVQTHQLGPVETLLVYRRDNRPSAFSAFHEVLSARKGREIRLATN
>Mature_301_residues
MDLSSIEIFLAVASDCSVTKAAKAVGRVPSNVTTRIQQLEEDLGASLFSRDGKKMTLTREGETFLTYANRLMALALEARQ
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPEVSLHLTMGASRDLARDVLADALDCALIARPPKTMREDDLEFDA
ELKALEKETVFVEDLLIVLPSGHPAIKSAADLRVGALAALEPGCTYRRIAENWARKSTALPTSELGSYHAILASVATGNT
AGVMPKSVLELMHWPTAVQTHQLGPVETLLVYRRDNRPSAFSAFHEVLSARKGREIRLATN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=308, Percent_Identity=36.3636363636364, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1790208, Length=255, Percent_Identity=28.2352941176471, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082132, Length=278, Percent_Identity=27.6978417266187, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787601, Length=301, Percent_Identity=24.9169435215947, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=181, Percent_Identity=27.6243093922652, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=144, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=147, Percent_Identity=27.2108843537415, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788887, Length=185, Percent_Identity=27.5675675675676, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=142, Percent_Identity=28.169014084507, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32694; Mature: 32694

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSSIEIFLAVASDCSVTKAAKAVGRVPSNVTTRIQQLEEDLGASLFSRDGKKMTLTRE
CCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEEEC
GETFLTYANRLMALALEARQAVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPEVSL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEE
HLTMGASRDLARDVLADALDCALIARPPKTMREDDLEFDAELKALEKETVFVEDLLIVLP
EEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEC
SGHPAIKSAADLRVGALAALEPGCTYRRIAENWARKSTALPTSELGSYHAILASVATGNT
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
AGVMPKSVLELMHWPTAVQTHQLGPVETLLVYRRDNRPSAFSAFHEVLSARKGREIRLAT
CCCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEC
N
C
>Mature Secondary Structure
MDLSSIEIFLAVASDCSVTKAAKAVGRVPSNVTTRIQQLEEDLGASLFSRDGKKMTLTRE
CCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEEEC
GETFLTYANRLMALALEARQAVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPEVSL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEE
HLTMGASRDLARDVLADALDCALIARPPKTMREDDLEFDAELKALEKETVFVEDLLIVLP
EEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEC
SGHPAIKSAADLRVGALAALEPGCTYRRIAENWARKSTALPTSELGSYHAILASVATGNT
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
AGVMPKSVLELMHWPTAVQTHQLGPVETLLVYRRDNRPSAFSAFHEVLSARKGREIRLAT
CCCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]