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The map label for this gene is appB [H]

Identifier: 190894757

GI number: 190894757

Start: 449942

End: 450928

Strand: Reverse

Name: appB [H]

Synonym: RHECIAT_PC0000422

Alternate gene names: 190894757

Gene position: 450928-449942 (Counterclockwise)

Preceding gene: 190894758

Following gene: 190894756

Centisome position: 41.31

GC content: 59.68

Gene sequence:

>987_bases
ATGCTGGTCTTTATCGCCAAACGCTTGCTATGGATGATTCCATCGCTTTTTGCCGTCAGTTTTCTCGCTTTCGTGCTGAT
CCAGCTGCCGCCGGGCGACTATGTCACCACCTATATCGCGACGCTGGCAGCCTCCAACGAGATCGTCGACCAGAACACCG
CGGCGCAGCTGCGCGAGCGCTTCGGCCTCGACGATCCGATGCTCATCCAATATTTCAAATGGATCTGGGGCATCCTCTCG
CGCGGCGATTTCGGCATCTCCTTCGAATGGCAGCAGCCGGTCTCCGACCTGATCTGGGAGCGCATGGCGCTGACCCTCGT
CCTGGCGCTTTCGACCTTGATCGCCACCTGGGCGATCGCTCTGCCGATCGGTGTCTTTTCCGCCGTGCGCAAATATTCGA
TCGGCGACTATTTCTTCACCGCCTTCACCTTTTTCGGTCTGGCCGTTCCCTCCTTCCTGCTGGCGCTGGTGCTGATGTAC
ATCGCGGCGGTCGAATTCGGCCAGGATGTCGGCGGGCTGTTTTCGCCGCAATACGAGAATGCGCCCTGGAGCTTCGCCAA
GATGGTCGATCTCTTCTCGCATCTCTGGCTTCCCGTCATCATTCTTGCCGTCTCCTCGACGGCGAGCCTCATCCGCGTCA
TGCGCGCCAACATGCTCGACGAACTGCCGAAGCCTTACGTGACGACGGCGCGGGCAAAGGGGCTCTCGGAGTTCCGTCTG
CTGATGAAATATCCTCTGATGATCGCGCTCAATCCGTTCATCTCGACGATCGCCTGGCTGCTGCCCAATCTCATCTCCGG
CTCGGTGGTCGTCGCCATCGTCCTCAATCTGCCGACGGCAGCACCCTTGCTGTTGCAGGCACTGATGGCCCAGGACATGT
ATCTGGCGGGCGCCTTCGTTCTGCTGATCTGCGCTCTGACGCTGATAGGCTCCCTGATCAGCGACATCCTGCTTGCGCTG
GTCGATCCCCGCATCCGGTTGGAATAG

Upstream 100 bases:

>100_bases
CCGACGGTACGGCGCTTTCTTCGGTTCGCCTTCAAAGATGCCTTGCCGGCGCTGATCGCCGACAGGGCCGGAGGGCGGCG
TTCAATGCAAGAGGCGCCTC

Downstream 100 bases:

>100_bases
GAGCCTGATATGGCCGACATCACCGCTACAAATCTGCAGCCGGACCGCGCCGCCGTTGCATCGCAGTGGCAGCTGATCTG
GTGGGCTTTCCGCCGGCATC

Product: putative oligopeptide ABC transporter permease

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLVFIAKRLLWMIPSLFAVSFLAFVLIQLPPGDYVTTYIATLAASNEIVDQNTAAQLRERFGLDDPMLIQYFKWIWGILS
RGDFGISFEWQQPVSDLIWERMALTLVLALSTLIATWAIALPIGVFSAVRKYSIGDYFFTAFTFFGLAVPSFLLALVLMY
IAAVEFGQDVGGLFSPQYENAPWSFAKMVDLFSHLWLPVIILAVSSTASLIRVMRANMLDELPKPYVTTARAKGLSEFRL
LMKYPLMIALNPFISTIAWLLPNLISGSVVVAIVLNLPTAAPLLLQALMAQDMYLAGAFVLLICALTLIGSLISDILLAL
VDPRIRLE

Sequences:

>Translated_328_residues
MLVFIAKRLLWMIPSLFAVSFLAFVLIQLPPGDYVTTYIATLAASNEIVDQNTAAQLRERFGLDDPMLIQYFKWIWGILS
RGDFGISFEWQQPVSDLIWERMALTLVLALSTLIATWAIALPIGVFSAVRKYSIGDYFFTAFTFFGLAVPSFLLALVLMY
IAAVEFGQDVGGLFSPQYENAPWSFAKMVDLFSHLWLPVIILAVSSTASLIRVMRANMLDELPKPYVTTARAKGLSEFRL
LMKYPLMIALNPFISTIAWLLPNLISGSVVVAIVLNLPTAAPLLLQALMAQDMYLAGAFVLLICALTLIGSLISDILLAL
VDPRIRLE
>Mature_328_residues
MLVFIAKRLLWMIPSLFAVSFLAFVLIQLPPGDYVTTYIATLAASNEIVDQNTAAQLRERFGLDDPMLIQYFKWIWGILS
RGDFGISFEWQQPVSDLIWERMALTLVLALSTLIATWAIALPIGVFSAVRKYSIGDYFFTAFTFFGLAVPSFLLALVLMY
IAAVEFGQDVGGLFSPQYENAPWSFAKMVDLFSHLWLPVIILAVSSTASLIRVMRANMLDELPKPYVTTARAKGLSEFRL
LMKYPLMIALNPFISTIAWLLPNLISGSVVVAIVLNLPTAAPLLLQALMAQDMYLAGAFVLLICALTLIGSLISDILLAL
VDPRIRLE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=342, Percent_Identity=29.8245614035088, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787053, Length=330, Percent_Identity=30.9090909090909, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787497, Length=327, Percent_Identity=28.1345565749235, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788504, Length=364, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789888, Length=326, Percent_Identity=29.4478527607362, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787550, Length=257, Percent_Identity=27.2373540856031, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787761, Length=322, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36439; Mature: 36439

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]