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The map label for this gene is lutR [H]

Identifier: 190894651

GI number: 190894651

Start: 337364

End: 338128

Strand: Reverse

Name: lutR [H]

Synonym: RHECIAT_PC0000314

Alternate gene names: 190894651

Gene position: 338128-337364 (Counterclockwise)

Preceding gene: 190894652

Following gene: 190894644

Centisome position: 30.98

GC content: 63.27

Gene sequence:

>765_bases
GTGACTGAAGCGAATGGCGAATCTTTGAACGCGGAAGCCGGCCCGCCGGGCAAGCCCGAACGCGGCAAGGCCGTCAAGCT
GGTCGATCGCGTCTTCGACCAGCTGCTCGAGCGGATCCGCGGCGGAAACTATCCGCCGGATTCACGCCTTCCGGGCGAAC
ACGAACTTGCCTCGATGCTCGGTGTATCCCGGCCGATCGTTCGCGACGCGCTGGCGCGCCTGCGCGACCAGGGCATCGTC
TATGCCCGCCAGGGGGCAGGCACCTTCGTCAGCGCGCATGGATCGCCGACGACGCAGCTCGCCTATTCGCCTGTCAAGAC
CATTGCCGATATTCAGCGCTGCTACGAATTCCGCCTGACCATCGAACCGGCCGCCGCCTATTTCGCCGCCAAGCGCCGTA
ATGAGCAGGCGATCCAGAAGATCGCCAGCGCTCTTGCGGATCTGCGCGAGGCGACCAGCCACCAGCTTCACCGCACCGAT
GCCGATTTCATTTTCCATCGCGCCGTGACCGAGGCTGCCAACAACCATTATTACACCGCCTCGATCGATGCGCTGAAAGC
CCATATCGCCGTCGGTATGCATCTCCACGGCCTCTCCCTGCTCGGCCCTCGTCAGGGGCTGGAGCAGGTCTATGAAGAGC
ATAACGCCATCTACAAGGCGATCGCCGATGGCCGCGCCGATGATGCGCAAAGGCTGATGAAGGCGCATCTCGAGGGCTCC
CGAGACCGTCTGTTTGAGGGCAGGGCGCTCGATCTGTCCTTCTGA

Upstream 100 bases:

>100_bases
GTGCGCCGTCAAGTCGGGGGGCTTCGGCAAGCCTGATAGTTTGCTAAGACTGGTTCTTCGGAATCAGGCGGCATAGAATG
ATACCGTCGGGGAGAATGAC

Downstream 100 bases:

>100_bases
GACGGTGAGACGTCGGCTTCGCGAATAGGTCGAGGCCTATCGCCGCGACAAGATTCCGGCAAAGAGCTGACGGAAGGTCC
CGGCGATCACGCCGGCTTCA

Product: putative GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTEANGESLNAEAGPPGKPERGKAVKLVDRVFDQLLERIRGGNYPPDSRLPGEHELASMLGVSRPIVRDALARLRDQGIV
YARQGAGTFVSAHGSPTTQLAYSPVKTIADIQRCYEFRLTIEPAAAYFAAKRRNEQAIQKIASALADLREATSHQLHRTD
ADFIFHRAVTEAANNHYYTASIDALKAHIAVGMHLHGLSLLGPRQGLEQVYEEHNAIYKAIADGRADDAQRLMKAHLEGS
RDRLFEGRALDLSF

Sequences:

>Translated_254_residues
MTEANGESLNAEAGPPGKPERGKAVKLVDRVFDQLLERIRGGNYPPDSRLPGEHELASMLGVSRPIVRDALARLRDQGIV
YARQGAGTFVSAHGSPTTQLAYSPVKTIADIQRCYEFRLTIEPAAAYFAAKRRNEQAIQKIASALADLREATSHQLHRTD
ADFIFHRAVTEAANNHYYTASIDALKAHIAVGMHLHGLSLLGPRQGLEQVYEEHNAIYKAIADGRADDAQRLMKAHLEGS
RDRLFEGRALDLSF
>Mature_253_residues
TEANGESLNAEAGPPGKPERGKAVKLVDRVFDQLLERIRGGNYPPDSRLPGEHELASMLGVSRPIVRDALARLRDQGIVY
ARQGAGTFVSAHGSPTTQLAYSPVKTIADIQRCYEFRLTIEPAAAYFAAKRRNEQAIQKIASALADLREATSHQLHRTDA
DFIFHRAVTEAANNHYYTASIDALKAHIAVGMHLHGLSLLGPRQGLEQVYEEHNAIYKAIADGRADDAQRLMKAHLEGSR
DRLFEGRALDLSF

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=223, Percent_Identity=32.7354260089686, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786303, Length=219, Percent_Identity=29.2237442922374, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790780, Length=219, Percent_Identity=31.5068493150685, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI48994955, Length=232, Percent_Identity=25.4310344827586, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082208, Length=229, Percent_Identity=27.0742358078603, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1789621, Length=221, Percent_Identity=27.1493212669683, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27992; Mature: 27861

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEANGESLNAEAGPPGKPERGKAVKLVDRVFDQLLERIRGGNYPPDSRLPGEHELASML
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
GVSRPIVRDALARLRDQGIVYARQGAGTFVSAHGSPTTQLAYSPVKTIADIQRCYEFRLT
CCCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEE
IEPAAAYFAAKRRNEQAIQKIASALADLREATSHQLHRTDADFIFHRAVTEAANNHYYTA
ECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEE
SIDALKAHIAVGMHLHGLSLLGPRQGLEQVYEEHNAIYKAIADGRADDAQRLMKAHLEGS
HHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RDRLFEGRALDLSF
HHHHHCCCEECCCC
>Mature Secondary Structure 
TEANGESLNAEAGPPGKPERGKAVKLVDRVFDQLLERIRGGNYPPDSRLPGEHELASML
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
GVSRPIVRDALARLRDQGIVYARQGAGTFVSAHGSPTTQLAYSPVKTIADIQRCYEFRLT
CCCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEE
IEPAAAYFAAKRRNEQAIQKIASALADLREATSHQLHRTDADFIFHRAVTEAANNHYYTA
ECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEE
SIDALKAHIAVGMHLHGLSLLGPRQGLEQVYEEHNAIYKAIADGRADDAQRLMKAHLEGS
HHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RDRLFEGRALDLSF
HHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]