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The map label for this gene is rhtB [C]

Identifier: 190894471

GI number: 190894471

Start: 132108

End: 132716

Strand: Reverse

Name: rhtB [C]

Synonym: RHECIAT_PC0000133

Alternate gene names: 190894471

Gene position: 132716-132108 (Counterclockwise)

Preceding gene: 190894473

Following gene: 190894463

Centisome position: 12.16

GC content: 60.26

Gene sequence:

>609_bases
ATGACGCTTTCCGGTTTTTTGGCCTATTCCGGCGCGTTGGCCATTGCCGCGATCATACCGGGACCGCAGATCGTTGCCAT
CGTCGCGCAGGCGCTGCACTCAGGCTATCGCAAGGCTGCATGGATGACTGCCGGCATGGTGGTTGGCGACGTGATCTATC
TTTCGGCCGCACTGGCAGGGCTTGCCTATATCGCAGCAACCTTCACTACGTTGCTAGTCGTCATCAAATGGGCTGGCGTC
GTCTATCTCTGCTGGCTCGCCTATCAATTCTGGATAGCTCCCAACTCGCTGCAGCCGCAGCATGGACCCTCCACCGCAGG
CGGCGTGTTCGCCTCCGGCATTCTGGTGACACTGGGCAATCCAAAATCGGTGTTGTTCTACGTGTCCATTCTGCCGACAG
TCGTGGACGTGAACGCGCTATCAGGCCTGGATATCACCCTGCTGCTTGCCATGACGGCCGTGATCCTCGCCGTTGCCCAG
TACCCCTTCGCTCTCGCGGGCACCCGGGTGCGAATGGCCCTGAGATCGCCGAGGGCGATGACTATGATGAACCGGAGTGC
TGCTTTTTGCATGGGAGGCGCAGCGGTGGCTATCGCGACCCGCAGCTAG

Upstream 100 bases:

>100_bases
ACGGACAGCGCTTTCCGGGCGCCGGCGGCAAGTTCCACTAGCCATTGGCTTTCGTCCGATAATCTGAATACTAATTTGCA
AAGTTTTGTTGGAGCTTGTG

Downstream 100 bases:

>100_bases
TCCTTGAACGGGGGACTCGAAGCTATGTCGCAACGACATCCATAGGCTAGCCATCCGATCGTCCCGCGTCTAACAGTTCG
CCCTCAGGAAACGCGGACGT

Product: putative amino acid efflux protein, LysE family

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTLSGFLAYSGALAIAAIIPGPQIVAIVAQALHSGYRKAAWMTAGMVVGDVIYLSAALAGLAYIAATFTTLLVVIKWAGV
VYLCWLAYQFWIAPNSLQPQHGPSTAGGVFASGILVTLGNPKSVLFYVSILPTVVDVNALSGLDITLLLAMTAVILAVAQ
YPFALAGTRVRMALRSPRAMTMMNRSAAFCMGGAAVAIATRS

Sequences:

>Translated_202_residues
MTLSGFLAYSGALAIAAIIPGPQIVAIVAQALHSGYRKAAWMTAGMVVGDVIYLSAALAGLAYIAATFTTLLVVIKWAGV
VYLCWLAYQFWIAPNSLQPQHGPSTAGGVFASGILVTLGNPKSVLFYVSILPTVVDVNALSGLDITLLLAMTAVILAVAQ
YPFALAGTRVRMALRSPRAMTMMNRSAAFCMGGAAVAIATRS
>Mature_201_residues
TLSGFLAYSGALAIAAIIPGPQIVAIVAQALHSGYRKAAWMTAGMVVGDVIYLSAALAGLAYIAATFTTLLVVIKWAGVV
YLCWLAYQFWIAPNSLQPQHGPSTAGGVFASGILVTLGNPKSVLFYVSILPTVVDVNALSGLDITLLLAMTAVILAVAQY
PFALAGTRVRMALRSPRAMTMMNRSAAFCMGGAAVAIATRS

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=26.6990291262136, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788099, Length=175, Percent_Identity=26.2857142857143, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21011; Mature: 20880

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSGFLAYSGALAIAAIIPGPQIVAIVAQALHSGYRKAAWMTAGMVVGDVIYLSAALAG
CCCCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYIAATFTTLLVVIKWAGVVYLCWLAYQFWIAPNSLQPQHGPSTAGGVFASGILVTLGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEEECC
PKSVLFYVSILPTVVDVNALSGLDITLLLAMTAVILAVAQYPFALAGTRVRMALRSPRAM
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
TMMNRSAAFCMGGAAVAIATRS
HHHCCCHHHEECCEEEEEEECC
>Mature Secondary Structure 
TLSGFLAYSGALAIAAIIPGPQIVAIVAQALHSGYRKAAWMTAGMVVGDVIYLSAALAG
CCCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYIAATFTTLLVVIKWAGVVYLCWLAYQFWIAPNSLQPQHGPSTAGGVFASGILVTLGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEEECC
PKSVLFYVSILPTVVDVNALSGLDITLLLAMTAVILAVAQYPFALAGTRVRMALRSPRAM
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
TMMNRSAAFCMGGAAVAIATRS
HHHCCCHHHEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]