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The map label for this gene is dapF [H]

Identifier: 190571354

GI number: 190571354

Start: 1026800

End: 1027594

Strand: Direct

Name: dapF [H]

Synonym: WPa_0959

Alternate gene names: 190571354

Gene position: 1026800-1027594 (Clockwise)

Preceding gene: 190571352

Following gene: 190571360

Centisome position: 69.26

GC content: 35.35

Gene sequence:

>795_bases
ATGGTAAGTAACCTAACAGACAAGATCCCGTTTGTAAAGATGCATGGTACTGGCAATAGTTTTGTTATCATAGACTCACG
TTCAGCAAATAATTTAGACTGGAATTATAGACAAATTGCTGATCAAGGCAGTTGTGATCAAGTGATAATTATAACAATGT
CTAATGCTGCAAACTGCTTTATGCATATCTACAATGCTGACGGTAGTCGAGCTGAAATGTGTGGAAACGCAGCACGCTGT
GTTGGGTATTTGATAATGTTAGAAAAAGGTACTGAATATATCACTATTGAGCTGATAAATAATCGTATCTTAGAATGTTT
TAAAGTAGGTGATAAATCAATAAAGGTCAATATGGGTAAACCACTGCTTAAATGGAATGAAATTCCTCTTTCTTGTGAAT
GCGATCCCCTTTATCTACCTATAGAGCTTGAAATGCTGAAAGAGCCAGTTGCAGTAAATATTGGTAACCCTCATATAGTT
TTTTTTGTTGATAACATAAGCGAAATACCATTGCAGAATTTAGGACCAAAGCTAGAAAATCACGAATTATTTCCTCAGAA
AACAAATGTTAGTATTGCACAAATAGAAAAATCTGGAGAAATAAACTTAAGAGTTTGGGAAAGAGGAACAGGTATTACTG
CTTCATGTGGTAGTGCGGCTTGTGCAGCATTTGTTGCATCTATACTTCGTAAGTGTTTGGCTACTAAACAAACTTCAGTA
CATTTACCAGGAGGTCATTTACTGATTGAGTGGTCAAAAAATATACTGATGACTGGGGATATAGGGTTTTTATGA

Upstream 100 bases:

>100_bases
TGTTCTCCGGTATTGACTTAGCACTCACATTACAGAAAACTATAGAATACAAAACAGCTGTAATTAGAAGAAAAATTTTC
ACTTATATAATTTTGCTATA

Downstream 100 bases:

>100_bases
CCTAGGCTAGGCACTGACACTCATTACTTCTAACACCTTTTTACCAATCACAGCAGGGGTTTCTGCAATCGCAATCCCAG
CACTGCGCATAACCTCTAAC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MVSNLTDKIPFVKMHGTGNSFVIIDSRSANNLDWNYRQIADQGSCDQVIIITMSNAANCFMHIYNADGSRAEMCGNAARC
VGYLIMLEKGTEYITIELINNRILECFKVGDKSIKVNMGKPLLKWNEIPLSCECDPLYLPIELEMLKEPVAVNIGNPHIV
FFVDNISEIPLQNLGPKLENHELFPQKTNVSIAQIEKSGEINLRVWERGTGITASCGSAACAAFVASILRKCLATKQTSV
HLPGGHLLIEWSKNILMTGDIGFL

Sequences:

>Translated_264_residues
MVSNLTDKIPFVKMHGTGNSFVIIDSRSANNLDWNYRQIADQGSCDQVIIITMSNAANCFMHIYNADGSRAEMCGNAARC
VGYLIMLEKGTEYITIELINNRILECFKVGDKSIKVNMGKPLLKWNEIPLSCECDPLYLPIELEMLKEPVAVNIGNPHIV
FFVDNISEIPLQNLGPKLENHELFPQKTNVSIAQIEKSGEINLRVWERGTGITASCGSAACAAFVASILRKCLATKQTSV
HLPGGHLLIEWSKNILMTGDIGFL
>Mature_264_residues
MVSNLTDKIPFVKMHGTGNSFVIIDSRSANNLDWNYRQIADQGSCDQVIIITMSNAANCFMHIYNADGSRAEMCGNAARC
VGYLIMLEKGTEYITIELINNRILECFKVGDKSIKVNMGKPLLKWNEIPLSCECDPLYLPIELEMLKEPVAVNIGNPHIV
FFVDNISEIPLQNLGPKLENHELFPQKTNVSIAQIEKSGEINLRVWERGTGITASCGSAACAAFVASILRKCLATKQTSV
HLPGGHLLIEWSKNILMTGDIGFL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=262, Percent_Identity=35.1145038167939, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29187; Mature: 29187

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSNLTDKIPFVKMHGTGNSFVIIDSRSANNLDWNYRQIADQGSCDQVIIITMSNAANCF
CCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCEEE
MHIYNADGSRAEMCGNAARCVGYLIMLEKGTEYITIELINNRILECFKVGDKSIKVNMGK
EEEECCCCCHHHHCCCHHHHEEEEEEEECCCCEEEEEECCHHHHHHHHCCCCEEEEECCC
PLLKWNEIPLSCECDPLYLPIELEMLKEPVAVNIGNPHIVFFVDNISEIPLQNLGPKLEN
CCEEECCCCCCCCCCCEEEEEEHHHCCCCEEEECCCCEEEEEECCCCCCCHHHCCCCCCC
HELFPQKTNVSIAQIEKSGEINLRVWERGTGITASCGSAACAAFVASILRKCLATKQTSV
CCCCCCCCCCEEEEEECCCCEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHCCCCCEE
HLPGGHLLIEWSKNILMTGDIGFL
ECCCCEEEEEECCCEEEECCCCCC
>Mature Secondary Structure
MVSNLTDKIPFVKMHGTGNSFVIIDSRSANNLDWNYRQIADQGSCDQVIIITMSNAANCF
CCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCEEE
MHIYNADGSRAEMCGNAARCVGYLIMLEKGTEYITIELINNRILECFKVGDKSIKVNMGK
EEEECCCCCHHHHCCCHHHHEEEEEEEECCCCEEEEEECCHHHHHHHHCCCCEEEEECCC
PLLKWNEIPLSCECDPLYLPIELEMLKEPVAVNIGNPHIVFFVDNISEIPLQNLGPKLEN
CCEEECCCCCCCCCCCEEEEEEHHHCCCCEEEECCCCEEEEEECCCCCCCHHHCCCCCCC
HELFPQKTNVSIAQIEKSGEINLRVWERGTGITASCGSAACAAFVASILRKCLATKQTSV
CCCCCCCCCCEEEEEECCCCEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHCCCCCEE
HLPGGHLLIEWSKNILMTGDIGFL
ECCCCEEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA