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The map label for this gene is truA

Identifier: 190571328

GI number: 190571328

Start: 1002261

End: 1002998

Strand: Direct

Name: truA

Synonym: WPa_0933

Alternate gene names: 190571328

Gene position: 1002261-1002998 (Clockwise)

Preceding gene: 190571326

Following gene: 190571335

Centisome position: 67.61

GC content: 37.4

Gene sequence:

>738_bases
GTGCGATACAAAATAACGATAGAATACAATGGTAGTAGTTTCTCTGGCTGGCAAAAGCAACAACACTCTGCTAACTCAAT
TCAGGAAACCATAGAAAACGCTATATTTAATTTCAGTGGCGAGAGAGTCTCTTTGCATTGTGGTGGCAGAACTGACACAG
GGGTTCATGCTTTAGGGCAAGTTGCTCATTTCAATATGGAAAGGCAATTTGAGCTTTACAGAATAAGAAATGGAATAAAC
TATCACTTAAAAGCGATTCCTATAGTTGTGCTTAGTGCAGAAGCCGTAGATGATGCATTTCATGCGCGATTTTCAGCAAA
AAAAAGATATTATGAATATAGAATAATTAATCGCTACGCTCCTGCAGCTCTGGAAATCGGTTATGTGTGGCAAGTATTTA
ACCCACTTGACGTAAACATCATGCGTGAAGCTGCTAGACACCTGCTTGGAAAACATAATCTTTCAAGCTTTCGCTCAAAA
GATTGTCAAGCTACAAATCCGGTCAGAACAATCGATGATATTGATATAGTACAAAACGGAAGTCACATATACATCAAAAT
ATCTGCGATTTCCTTTTTACATAACCAGGTGAGGATTATTGTTGGTACTTTAGTTGAATTTGGAAAAAATAGAACTAATC
CACAAGAAATGCTAAACATATTAAGTCAATGTAAAAGAAACGCTGCTGGAATCACTGCACCGCCTCACGGCTTATACTTA
GTAAAGATAGATTACTAG

Upstream 100 bases:

>100_bases
CTTGTATAGTCTCCATGGTAATTGAACGCAACCAATCTGCCAAGGGTTTTTTAATAACGAAGAACGTTAGTTACCTAAAA
TTAGATAGATAGACTTTGTA

Downstream 100 bases:

>100_bases
CTTAAGTCCTCATTGGCATTACTATATATAGTGCACTTGGATCATCTTCATCAGTGATAATTGTAGCGCTATTACCATCT
GATAAGCTAAATTTACACTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRYKITIEYNGSSFSGWQKQQHSANSIQETIENAIFNFSGERVSLHCGGRTDTGVHALGQVAHFNMERQFELYRIRNGIN
YHLKAIPIVVLSAEAVDDAFHARFSAKKRYYEYRIINRYAPAALEIGYVWQVFNPLDVNIMREAARHLLGKHNLSSFRSK
DCQATNPVRTIDDIDIVQNGSHIYIKISAISFLHNQVRIIVGTLVEFGKNRTNPQEMLNILSQCKRNAAGITAPPHGLYL
VKIDY

Sequences:

>Translated_245_residues
MRYKITIEYNGSSFSGWQKQQHSANSIQETIENAIFNFSGERVSLHCGGRTDTGVHALGQVAHFNMERQFELYRIRNGIN
YHLKAIPIVVLSAEAVDDAFHARFSAKKRYYEYRIINRYAPAALEIGYVWQVFNPLDVNIMREAARHLLGKHNLSSFRSK
DCQATNPVRTIDDIDIVQNGSHIYIKISAISFLHNQVRIIVGTLVEFGKNRTNPQEMLNILSQCKRNAAGITAPPHGLYL
VKIDY
>Mature_245_residues
MRYKITIEYNGSSFSGWQKQQHSANSIQETIENAIFNFSGERVSLHCGGRTDTGVHALGQVAHFNMERQFELYRIRNGIN
YHLKAIPIVVLSAEAVDDAFHARFSAKKRYYEYRIINRYAPAALEIGYVWQVFNPLDVNIMREAARHLLGKHNLSSFRSK
DCQATNPVRTIDDIDIVQNGSHIYIKISAISFLHNQVRIIVGTLVEFGKNRTNPQEMLNILSQCKRNAAGITAPPHGLYL
VKIDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=30.5147058823529, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=39.8373983739837, Blast_Score=169, Evalue=1e-43,
Organism=Drosophila melanogaster, GI116007752, Length=300, Percent_Identity=28.6666666666667, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_WOLPP (B3CMC1)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975686.1
- EnsemblBacteria:   EBWOLT00000000231
- GeneID:   6384545
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0933
- GeneTree:   EBGT00050000030940
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27939; Mature: 27939

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKITIEYNGSSFSGWQKQQHSANSIQETIENAIFNFSGERVSLHCGGRTDTGVHALGQ
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
VAHFNMERQFELYRIRNGINYHLKAIPIVVLSAEAVDDAFHARFSAKKRYYEYRIINRYA
HHHCCCHHHHHHEEHHCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
PAALEIGYVWQVFNPLDVNIMREAARHLLGKHNLSSFRSKDCQATNPVRTIDDIDIVQNG
CCCEEEEEHEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEECC
SHIYIKISAISFLHNQVRIIVGTLVEFGKNRTNPQEMLNILSQCKRNAAGITAPPHGLYL
CEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
VKIDY
EEECC
>Mature Secondary Structure
MRYKITIEYNGSSFSGWQKQQHSANSIQETIENAIFNFSGERVSLHCGGRTDTGVHALGQ
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
VAHFNMERQFELYRIRNGINYHLKAIPIVVLSAEAVDDAFHARFSAKKRYYEYRIINRYA
HHHCCCHHHHHHEEHHCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
PAALEIGYVWQVFNPLDVNIMREAARHLLGKHNLSSFRSKDCQATNPVRTIDDIDIVQNG
CCCEEEEEHEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEECC
SHIYIKISAISFLHNQVRIIVGTLVEFGKNRTNPQEMLNILSQCKRNAAGITAPPHGLYL
CEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
VKIDY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA