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The map label for this gene is mraW

Identifier: 190571322

GI number: 190571322

Start: 996591

End: 997475

Strand: Direct

Name: mraW

Synonym: WPa_0927

Alternate gene names: 190571322

Gene position: 996591-997475 (Clockwise)

Preceding gene: 190571320

Following gene: 190571325

Centisome position: 67.23

GC content: 35.37

Gene sequence:

>885_bases
ATGACACATATTCCAGTTTTGTTGAAAGAGATGCTACTGCAATTGTCACCACACAGTGGTGGTATATATGTGGATGCCAC
GTTTGGAGCTGGAGGATATAGCAAAGCAATATTGGAATCCGCTGATTGCAAAGTGTATGCAGTTGATAGAGATGAAACAG
TTACTAAATTTTATGATGATCTAAATGTTAAATACCCTAATAGAATAAAGTTATTTATTGAAAAGTTTAGTAATATCAAA
AATTTATTAGATAGTAATAACATTAAAGGTATCAATGGAATAGTTTTCGACGTTGGAGTTTCATCTATGCAGCTTGATAA
TGGAGATAGAGGATTCTCATTTCTACGTGATGGCCCACTTAACATGAGCATGGATAACTATTCTCATATGAATGCTTCGA
CGTTTGTTAACGCTTTACGTGAAGAAGAGATTGCGAACACTATATATAATTATGGGGGTGAGCGTCATTCCCGCAGAATC
GCAAGAGCGATAGTAAATGCGCGGAAGAAGAAAATTATCAAAACTACATTTGAACTTGCGGATATTGTACGTTCTGTAGT
GTTTCGTGGAAAAAGCAAGATTGACCCTGCAACTAGGACATTTCAGGCAATCAGAATATGGGTAAATGATGAGCTAGGAG
AGCTTGAAAAGGGTATTAAAGCTGCATCTGAGATTTTAAGTGAGAACGGCAAATTAATTGTAGTTACTTTTCATTCTTTA
GAAGATCGTATAGTCAAAACTGTTTTTAAAGATTTATGTGAACCAGGATCTACTAAAACATTCTCCCTTTTAAATAAAAA
AGTGATCAAAGCAAGTTCAGAAGAAATAAATGTAAATCCACGCGCGCGTTCAGCAAAACTGAGAGCTATACAAAGGTTAT
CATGA

Upstream 100 bases:

>100_bases
TCGTTGATTATATTTAAATATTCTTATACTATACCTATATATTTCCATATATTTTTTGGCGAGAAAGTTATTTTAATTTA
AGTGCACAAAGTCTCTTGAT

Downstream 100 bases:

>100_bases
GGACCTTTCATGCCATTTCAATAATTATATTTTTATTGTAGGGTTATTTAAGGTAAAATTACACGTTCAATCACTAAATA
GAGAACTAGCATGAATAAAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTHIPVLLKEMLLQLSPHSGGIYVDATFGAGGYSKAILESADCKVYAVDRDETVTKFYDDLNVKYPNRIKLFIEKFSNIK
NLLDSNNIKGINGIVFDVGVSSMQLDNGDRGFSFLRDGPLNMSMDNYSHMNASTFVNALREEEIANTIYNYGGERHSRRI
ARAIVNARKKKIIKTTFELADIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
EDRIVKTVFKDLCEPGSTKTFSLLNKKVIKASSEEINVNPRARSAKLRAIQRLS

Sequences:

>Translated_294_residues
MTHIPVLLKEMLLQLSPHSGGIYVDATFGAGGYSKAILESADCKVYAVDRDETVTKFYDDLNVKYPNRIKLFIEKFSNIK
NLLDSNNIKGINGIVFDVGVSSMQLDNGDRGFSFLRDGPLNMSMDNYSHMNASTFVNALREEEIANTIYNYGGERHSRRI
ARAIVNARKKKIIKTTFELADIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
EDRIVKTVFKDLCEPGSTKTFSLLNKKVIKASSEEINVNPRARSAKLRAIQRLS
>Mature_293_residues
THIPVLLKEMLLQLSPHSGGIYVDATFGAGGYSKAILESADCKVYAVDRDETVTKFYDDLNVKYPNRIKLFIEKFSNIKN
LLDSNNIKGINGIVFDVGVSSMQLDNGDRGFSFLRDGPLNMSMDNYSHMNASTFVNALREEEIANTIYNYGGERHSRRIA
RAIVNARKKKIIKTTFELADIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSLE
DRIVKTVFKDLCEPGSTKTFSLLNKKVIKASSEEINVNPRARSAKLRAIQRLS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=38.3928571428571, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=42.7807486631016, Blast_Score=152, Evalue=2e-37,
Organism=Escherichia coli, GI1786270, Length=310, Percent_Identity=38.3870967741935, Blast_Score=202, Evalue=2e-53,
Organism=Drosophila melanogaster, GI62472493, Length=326, Percent_Identity=37.1165644171779, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28571637, Length=326, Percent_Identity=37.1165644171779, Blast_Score=194, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_WOLPP (B3CMB5)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975680.1
- ProteinModelPortal:   B3CMB5
- EnsemblBacteria:   EBWOLT00000000513
- GeneID:   6385610
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0927
- GeneTree:   EBGT00050000031416
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 32983; Mature: 32852

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: BINDING 49-49 BINDING 76-76 BINDING 97-97 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHIPVLLKEMLLQLSPHSGGIYVDATFGAGGYSKAILESADCKVYAVDRDETVTKFYDD
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHC
LNVKYPNRIKLFIEKFSNIKNLLDSNNIKGINGIVFDVGVSSMQLDNGDRGFSFLRDGPL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCHHHCCCCC
NMSMDNYSHMNASTFVNALREEEIANTIYNYGGERHSRRIARAIVNARKKKIIKTTFELA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
HHHHHHHHCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCH
EDRIVKTVFKDLCEPGSTKTFSLLNKKVIKASSEEINVNPRARSAKLRAIQRLS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
THIPVLLKEMLLQLSPHSGGIYVDATFGAGGYSKAILESADCKVYAVDRDETVTKFYDD
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHC
LNVKYPNRIKLFIEKFSNIKNLLDSNNIKGINGIVFDVGVSSMQLDNGDRGFSFLRDGPL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCHHHCCCCC
NMSMDNYSHMNASTFVNALREEEIANTIYNYGGERHSRRIARAIVNARKKKIIKTTFELA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
HHHHHHHHCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCH
EDRIVKTVFKDLCEPGSTKTFSLLNKKVIKASSEEINVNPRARSAKLRAIQRLS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA