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The map label for this gene is purC

Identifier: 190571274

GI number: 190571274

Start: 944262

End: 944984

Strand: Reverse

Name: purC

Synonym: WPa_0875

Alternate gene names: 190571274

Gene position: 944984-944262 (Counterclockwise)

Preceding gene: 190571277

Following gene: 190571272

Centisome position: 63.74

GC content: 32.64

Gene sequence:

>723_bases
ATGTCACTGAATAAAACAATATACGAAGGTAAAGCCAAAGTCATTATTGAAACTGAAGACTCATCAACTGTTATACAGTA
CTTTAAAGATGATGTTACAGCGTTCAACAAGGAAAAATATGAAATTATTGAGGGCAAAGGAATAATTAATAATCATGTTA
GTGCTTTTATCATGGAAAAGCTTGAAGAAGCAGGAATTAGCACGCACTTTATAAACACTCTAAACGAAAGAGAACAGCTA
GTCAAAAAGCTCAAGATAATACCTCTTGAGGTAGTGGTTAGAAACATTGCAGCTGGCAGCTTTTGCAAACGTTTTAATAT
CAAAGAAGGTGAGGCACTTACATCTCCTATAATCGAGTTTTTTTACAAAAATGATGACCTAGCCGACCCAATGGTGAATG
AAAATCACATATTATATTTTGGTTGGTTATCCCATAAAGAAATGGAAGAAGTTAAAACTACAACTTTAAAGATCAACAAA
ATCTTAGTCGACCTATTTTCAAATGCAAATATATATTTAGTTGATCTCAAGTTAGAATTTGGCAAATTGATAAACAACAG
CACTAAAATCATTTTAGCCGATGAAATCAGCCCTGATAATTGTAGGCTATGGGATAAAAATACTCATAAAAAGCTAGACA
AAGATGTTTTCCGTTTAAATCTAGGAAACTTAAAGGAAGCATACTCAGAAGTTGCAAAAAGACTGTCGGTAAAGTTAGAC
TAA

Upstream 100 bases:

>100_bases
GGAAATATGGTTAATATTGTAACACTAAACGTCATTAGCAAATTTGTAGTAAACTAAGCAGCAATTTGATATTATCTTAT
AGTATACAGCTAAATTAATC

Downstream 100 bases:

>100_bases
CTAAATACTACTCTATCAAGTGGAGGCTGACATGCTTCATCAAGTTTTCCAATAATCTCAATTTTTTCTCCACGCTTTTG
GAAATGGCTTTCCACATCCT

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSLNKTIYEGKAKVIIETEDSSTVIQYFKDDVTAFNKEKYEIIEGKGIINNHVSAFIMEKLEEAGISTHFINTLNEREQL
VKKLKIIPLEVVVRNIAAGSFCKRFNIKEGEALTSPIIEFFYKNDDLADPMVNENHILYFGWLSHKEMEEVKTTTLKINK
ILVDLFSNANIYLVDLKLEFGKLINNSTKIILADEISPDNCRLWDKNTHKKLDKDVFRLNLGNLKEAYSEVAKRLSVKLD

Sequences:

>Translated_240_residues
MSLNKTIYEGKAKVIIETEDSSTVIQYFKDDVTAFNKEKYEIIEGKGIINNHVSAFIMEKLEEAGISTHFINTLNEREQL
VKKLKIIPLEVVVRNIAAGSFCKRFNIKEGEALTSPIIEFFYKNDDLADPMVNENHILYFGWLSHKEMEEVKTTTLKINK
ILVDLFSNANIYLVDLKLEFGKLINNSTKIILADEISPDNCRLWDKNTHKKLDKDVFRLNLGNLKEAYSEVAKRLSVKLD
>Mature_239_residues
SLNKTIYEGKAKVIIETEDSSTVIQYFKDDVTAFNKEKYEIIEGKGIINNHVSAFIMEKLEEAGISTHFINTLNEREQLV
KKLKIIPLEVVVRNIAAGSFCKRFNIKEGEALTSPIIEFFYKNDDLADPMVNENHILYFGWLSHKEMEEVKTTTLKINKI
LVDLFSNANIYLVDLKLEFGKLINNSTKIILADEISPDNCRLWDKNTHKKLDKDVFRLNLGNLKEAYSEVAKRLSVKLD

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=257, Percent_Identity=34.6303501945525, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI5453539, Length=257, Percent_Identity=34.6303501945525, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI119220559, Length=264, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=5e-25,
Organism=Escherichia coli, GI1788820, Length=240, Percent_Identity=46.25, Blast_Score=218, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17531275, Length=221, Percent_Identity=32.579185520362, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18860083, Length=241, Percent_Identity=31.9502074688797, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583917, Length=251, Percent_Identity=29.0836653386454, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_WOLPP (B3CM64)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975632.1
- ProteinModelPortal:   B3CM64
- EnsemblBacteria:   EBWOLT00000000978
- GeneID:   6384535
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0875
- GeneTree:   EBGT00050000031223
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- ProtClustDB:   PRK09362
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 27606; Mature: 27475

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNKTIYEGKAKVIIETEDSSTVIQYFKDDVTAFNKEKYEIIEGKGIINNHVSAFIMEK
CCCCCCEECCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
LEEAGISTHFINTLNEREQLVKKLKIIPLEVVVRNIAAGSFCKRFNIKEGEALTSPIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FYKNDDLADPMVNENHILYFGWLSHKEMEEVKTTTLKINKILVDLFSNANIYLVDLKLEF
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHH
GKLINNSTKIILADEISPDNCRLWDKNTHKKLDKDVFRLNLGNLKEAYSEVAKRLSVKLD
HHHCCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLNKTIYEGKAKVIIETEDSSTVIQYFKDDVTAFNKEKYEIIEGKGIINNHVSAFIMEK
CCCCCEECCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
LEEAGISTHFINTLNEREQLVKKLKIIPLEVVVRNIAAGSFCKRFNIKEGEALTSPIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FYKNDDLADPMVNENHILYFGWLSHKEMEEVKTTTLKINKILVDLFSNANIYLVDLKLEF
HHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHH
GKLINNSTKIILADEISPDNCRLWDKNTHKKLDKDVFRLNLGNLKEAYSEVAKRLSVKLD
HHHCCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA