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The map label for this gene is rnhB

Identifier: 190571269

GI number: 190571269

Start: 940750

End: 941343

Strand: Direct

Name: rnhB

Synonym: WPa_0870

Alternate gene names: 190571269

Gene position: 940750-941343 (Clockwise)

Preceding gene: 190571268

Following gene: 190571273

Centisome position: 63.46

GC content: 35.69

Gene sequence:

>594_bases
ATGAAATATCCAGACTTTTCATTAGAAAATACATTATCAGGAATTATAGCAGGAGTAGATGAAGTTGGAAGAGGTCCGCT
TGCCGGTCCAGTGATATCTGCAGCTGTAATATTTACTGATAGAGATACGGTTATTGATGGAATAAACGACTCAAAAAAGC
TAACCCCTAAGTGTAGGCAAGTTCTATACGAAAAAATAACGTCTGTTGCAAAATTTGGTATAGGAATGGCAAGCGTAGAA
GAAATAAACTCATACAATATTTTGCAAGCAACAAAGCTTTCAATGAAACGTGCGTTGGCAAACTTGAATCTAGAACTAGA
TTATGTGCTAGTTGATGGTAATCAACCACCTGAAGTAAAATGGCAAGTAAAATCTATAGTGAATGGTGATAGCTTAAGTA
TATCAATCGCAGCTGCTTCAATCGTTGCAAAAGTTACAAGAGATCGGCTTATGGAAGAATTGCATAATAAACATCCTCAA
TATAATTGGTATAAAAATAAGGGATATGGAACAAAAGAACACCTCAGCGCTATTGGCCTTCATGGAATTACGAAGCACCA
TAGAAAGAATTTTGCACCTATAAGAATACTATAG

Upstream 100 bases:

>100_bases
TTGAAAAGAAAGGGTGGTCAGAAAATACAATAGTTATAAAATTTTGGATAATTTCTATTGCTTGTTCGATTTTTGCTTTA
ACTTTCTTATTATAGGAATT

Downstream 100 bases:

>100_bases
CCTTTAATCACTTACCTTTTTTGAAAGGTATGTTTTCATTAAACAGGCAGACTGTGCTTGATCCGTTTCTTAAGGTAAAT
ACCGAGCTTACTCTTTCAAT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKYPDFSLENTLSGIIAGVDEVGRGPLAGPVISAAVIFTDRDTVIDGINDSKKLTPKCRQVLYEKITSVAKFGIGMASVE
EINSYNILQATKLSMKRALANLNLELDYVLVDGNQPPEVKWQVKSIVNGDSLSISIAAASIVAKVTRDRLMEELHNKHPQ
YNWYKNKGYGTKEHLSAIGLHGITKHHRKNFAPIRIL

Sequences:

>Translated_197_residues
MKYPDFSLENTLSGIIAGVDEVGRGPLAGPVISAAVIFTDRDTVIDGINDSKKLTPKCRQVLYEKITSVAKFGIGMASVE
EINSYNILQATKLSMKRALANLNLELDYVLVDGNQPPEVKWQVKSIVNGDSLSISIAAASIVAKVTRDRLMEELHNKHPQ
YNWYKNKGYGTKEHLSAIGLHGITKHHRKNFAPIRIL
>Mature_197_residues
MKYPDFSLENTLSGIIAGVDEVGRGPLAGPVISAAVIFTDRDTVIDGINDSKKLTPKCRQVLYEKITSVAKFGIGMASVE
EINSYNILQATKLSMKRALANLNLELDYVLVDGNQPPEVKWQVKSIVNGDSLSISIAAASIVAKVTRDRLMEELHNKHPQ
YNWYKNKGYGTKEHLSAIGLHGITKHHRKNFAPIRIL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=182, Percent_Identity=48.9010989010989, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_WOLPP (B3CM59)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975627.1
- EnsemblBacteria:   EBWOLT00000000907
- GeneID:   6384549
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0870
- GeneTree:   EBGT00050000031365
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21784; Mature: 21784

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYPDFSLENTLSGIIAGVDEVGRGPLAGPVISAAVIFTDRDTVIDGINDSKKLTPKCRQ
CCCCCCCHHHHHHHHHHCHHHHCCCCCCCHHHEEEEEEECCCHHCCCCCCCCCCCHHHHH
VLYEKITSVAKFGIGMASVEEINSYNILQATKLSMKRALANLNLELDYVLVDGNQPPEVK
HHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCE
WQVKSIVNGDSLSISIAAASIVAKVTRDRLMEELHNKHPQYNWYKNKGYGTKEHLSAIGL
EEEEHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
HGITKHHRKNFAPIRIL
HHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MKYPDFSLENTLSGIIAGVDEVGRGPLAGPVISAAVIFTDRDTVIDGINDSKKLTPKCRQ
CCCCCCCHHHHHHHHHHCHHHHCCCCCCCHHHEEEEEEECCCHHCCCCCCCCCCCHHHHH
VLYEKITSVAKFGIGMASVEEINSYNILQATKLSMKRALANLNLELDYVLVDGNQPPEVK
HHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCE
WQVKSIVNGDSLSISIAAASIVAKVTRDRLMEELHNKHPQYNWYKNKGYGTKEHLSAIGL
EEEEHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
HGITKHHRKNFAPIRIL
HHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA