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The map label for this gene is murF [H]

Identifier: 190571247

GI number: 190571247

Start: 924480

End: 925856

Strand: Reverse

Name: murF [H]

Synonym: WPa_0848

Alternate gene names: 190571247

Gene position: 925856-924480 (Counterclockwise)

Preceding gene: 190571255

Following gene: 190571246

Centisome position: 62.45

GC content: 32.1

Gene sequence:

>1377_bases
ATGTTAAAATGGAACGCAAATAATATTATAGATGCTACTGGCGGAAGAGGGGAGGGTGTTTGTAATTCGGTGTATAGCTC
AAATATTTCAACGGATACAAGAAGCATAAAAAAAGGTGATTTATTTATTGCTCTCAAGGGAAAGAAATTTGATGGGCATG
ATTTTCTACATGAAGCATTTTTAAAAGGAGCTGTTGCTGCGGTAGTAAGCGAAGATAAATATAGAAATTTTCCTCTAATT
ATTGTGCAAGATACTCTAAAAGCTTTGCATAATATGGCATCATATTACATTAGAAATATTCTTGTTAATGCTAAAGTTAT
CACAATTACAGGTAGTGTTGGGAAAACCACTACAAAAGATATGCTTAACACTGTTTTGTTGCATTATGGAGTATCTCATG
TAAATGAAGGTAACTTGAATAATAATATAGGATTGCCTTTTACGATTCTAAAAGCGCCAGAAGACTGCCAGTATTTAATC
CTTGAAATAGGAATGAGTAGAGCTGGTGAAATAAAAGAGTTATCGAAGATTAGCAATCCGGATATTGCAGTTATTACCAA
CATTGAACCTGCGCATGTTGAAAACTTTTCATCTCTACTTGATATTGCGCAAGCGAAATTAGAAATTCTACATGGTATGA
AAAATAATGGTACTTTGGTTTTGAATAAGGATAATGAACATTATGATTATCTCTTATCAAATGCTAACAGAAATGTAGTA
AGCTTTGGTAAGAATGAAAGTGCTACTGTTTGTTTGTTAGAACTAATAAGAGACGATAACGGTCTAAATTTAAAAATCAA
ACTGAACAGTGGTCAAATTATGAACTGTAATTTACCTGTAAAAGGCGAACATTTTGCATACTCTGCATTAGTTGTTACAG
CAGTTTTGCAAAGTCTCGAACTTGATTTATCTAAATTACCACTAGCACTTGAGAATTTTACTGTAACAGAAGGTAGAGGT
AATGTTCATCAAGCTAAATACAATGGAAAATTAGTACATTTGATTAACGACTCCTATAATGCTAACCCTACTTCAATGAA
GGCTGCAATAAGAACTTTAGGTACATATTCTAATTTAAGAAAAGTGGCACTACTTGGCGATATGTTAGGACTTGGTAATG
AAAGCATAGTATTTCATACAGAATTGCTTGACTCTATAGTAGAACAAAATGTGAATAAAGTTCATACAGTTGGTAAATTT
ATGTTAGAATTGAATAAACTTTTACCGGAGAATATAAAAGGCATGCATTTCGATGATTCTAACCAATTGAAAAGTAATTT
AGCTAATATTGTTCAAGGTAATGATGTAATTTTAGTTAAAGGTTCTCATGGAATGAGAATGGACCTTGTAGTGCAGGAAT
TCACAATAGAATATTAA

Upstream 100 bases:

>100_bases
TAAGATGAAATTATAGAAATAGCAATCATTATTTGTACATTACCAAAGTATGTAGTATTTTTGCTGCTTTTCTTGTTCAA
ATTACCTTTAAAGATAAATT

Downstream 100 bases:

>100_bases
ATAAAACTATTGTATTAATTTAAAGTTAAGTTATTGGAAGTTTTAACTGTGATAAAACGTATAATTATTTTTGGTGGAAC
GGGATTTATAGGAAAACACA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MLKWNANNIIDATGGRGEGVCNSVYSSNISTDTRSIKKGDLFIALKGKKFDGHDFLHEAFLKGAVAAVVSEDKYRNFPLI
IVQDTLKALHNMASYYIRNILVNAKVITITGSVGKTTTKDMLNTVLLHYGVSHVNEGNLNNNIGLPFTILKAPEDCQYLI
LEIGMSRAGEIKELSKISNPDIAVITNIEPAHVENFSSLLDIAQAKLEILHGMKNNGTLVLNKDNEHYDYLLSNANRNVV
SFGKNESATVCLLELIRDDNGLNLKIKLNSGQIMNCNLPVKGEHFAYSALVVTAVLQSLELDLSKLPLALENFTVTEGRG
NVHQAKYNGKLVHLINDSYNANPTSMKAAIRTLGTYSNLRKVALLGDMLGLGNESIVFHTELLDSIVEQNVNKVHTVGKF
MLELNKLLPENIKGMHFDDSNQLKSNLANIVQGNDVILVKGSHGMRMDLVVQEFTIEY

Sequences:

>Translated_458_residues
MLKWNANNIIDATGGRGEGVCNSVYSSNISTDTRSIKKGDLFIALKGKKFDGHDFLHEAFLKGAVAAVVSEDKYRNFPLI
IVQDTLKALHNMASYYIRNILVNAKVITITGSVGKTTTKDMLNTVLLHYGVSHVNEGNLNNNIGLPFTILKAPEDCQYLI
LEIGMSRAGEIKELSKISNPDIAVITNIEPAHVENFSSLLDIAQAKLEILHGMKNNGTLVLNKDNEHYDYLLSNANRNVV
SFGKNESATVCLLELIRDDNGLNLKIKLNSGQIMNCNLPVKGEHFAYSALVVTAVLQSLELDLSKLPLALENFTVTEGRG
NVHQAKYNGKLVHLINDSYNANPTSMKAAIRTLGTYSNLRKVALLGDMLGLGNESIVFHTELLDSIVEQNVNKVHTVGKF
MLELNKLLPENIKGMHFDDSNQLKSNLANIVQGNDVILVKGSHGMRMDLVVQEFTIEY
>Mature_458_residues
MLKWNANNIIDATGGRGEGVCNSVYSSNISTDTRSIKKGDLFIALKGKKFDGHDFLHEAFLKGAVAAVVSEDKYRNFPLI
IVQDTLKALHNMASYYIRNILVNAKVITITGSVGKTTTKDMLNTVLLHYGVSHVNEGNLNNNIGLPFTILKAPEDCQYLI
LEIGMSRAGEIKELSKISNPDIAVITNIEPAHVENFSSLLDIAQAKLEILHGMKNNGTLVLNKDNEHYDYLLSNANRNVV
SFGKNESATVCLLELIRDDNGLNLKIKLNSGQIMNCNLPVKGEHFAYSALVVTAVLQSLELDLSKLPLALENFTVTEGRG
NVHQAKYNGKLVHLINDSYNANPTSMKAAIRTLGTYSNLRKVALLGDMLGLGNESIVFHTELLDSIVEQNVNKVHTVGKF
MLELNKLLPENIKGMHFDDSNQLKSNLANIVQGNDVILVKGSHGMRMDLVVQEFTIEY

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=430, Percent_Identity=33.0232558139535, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50475; Mature: 50475

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKWNANNIIDATGGRGEGVCNSVYSSNISTDTRSIKKGDLFIALKGKKFDGHDFLHEAF
CEEECCCCEEECCCCCCCCHHHHHHHCCCCCCHHCCCCCCEEEEEECCCCCCHHHHHHHH
LKGAVAAVVSEDKYRNFPLIIVQDTLKALHNMASYYIRNILVNAKVITITGSVGKTTTKD
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
MLNTVLLHYGVSHVNEGNLNNNIGLPFTILKAPEDCQYLILEIGMSRAGEIKELSKISNP
HHHHHHHHHCHHHCCCCCCCCCCCCCEEEEECCCCHHEEEEECCCCCCCCHHHHHHCCCC
DIAVITNIEPAHVENFSSLLDIAQAKLEILHGMKNNGTLVLNKDNEHYDYLLSNANRNVV
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHEECCCCCEE
SFGKNESATVCLLELIRDDNGLNLKIKLNSGQIMNCNLPVKGEHFAYSALVVTAVLQSLE
EECCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LDLSKLPLALENFTVTEGRGNVHQAKYNGKLVHLINDSYNANPTSMKAAIRTLGTYSNLR
CCHHHCCEEECCEEEECCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KVALLGDMLGLGNESIVFHTELLDSIVEQNVNKVHTVGKFMLELNKLLPENIKGMHFDDS
HHHHHHHHHCCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCCH
NQLKSNLANIVQGNDVILVKGSHGMRMDLVVQEFTIEY
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEECC
>Mature Secondary Structure
MLKWNANNIIDATGGRGEGVCNSVYSSNISTDTRSIKKGDLFIALKGKKFDGHDFLHEAF
CEEECCCCEEECCCCCCCCHHHHHHHCCCCCCHHCCCCCCEEEEEECCCCCCHHHHHHHH
LKGAVAAVVSEDKYRNFPLIIVQDTLKALHNMASYYIRNILVNAKVITITGSVGKTTTKD
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
MLNTVLLHYGVSHVNEGNLNNNIGLPFTILKAPEDCQYLILEIGMSRAGEIKELSKISNP
HHHHHHHHHCHHHCCCCCCCCCCCCCEEEEECCCCHHEEEEECCCCCCCCHHHHHHCCCC
DIAVITNIEPAHVENFSSLLDIAQAKLEILHGMKNNGTLVLNKDNEHYDYLLSNANRNVV
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHEECCCCCEE
SFGKNESATVCLLELIRDDNGLNLKIKLNSGQIMNCNLPVKGEHFAYSALVVTAVLQSLE
EECCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LDLSKLPLALENFTVTEGRGNVHQAKYNGKLVHLINDSYNANPTSMKAAIRTLGTYSNLR
CCHHHCCEEECCEEEECCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KVALLGDMLGLGNESIVFHTELLDSIVEQNVNKVHTVGKFMLELNKLLPENIKGMHFDDS
HHHHHHHHHCCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCCH
NQLKSNLANIVQGNDVILVKGSHGMRMDLVVQEFTIEY
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]