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The map label for this gene is pnp

Identifier: 190571231

GI number: 190571231

Start: 907274

End: 909550

Strand: Direct

Name: pnp

Synonym: WPa_0831

Alternate gene names: 190571231

Gene position: 907274-909550 (Clockwise)

Preceding gene: 190571230

Following gene: 190571239

Centisome position: 61.2

GC content: 35.35

Gene sequence:

>2277_bases
ATGTTTGAAATTATAAAAAAATCTATAGATTGGGAGGGGCGTACCTTATCTTTAGAAACTGGGAAAATAGCACGTCAAGC
TGATGGTTCAGTAGTTGTAAATTATGGAGATACTTCTATCTTAGTCACTGTTGTACGAAAAAAGAAGGAAGAAAGTGTTG
ATTTTCTCCCTTTAAATGTGCAGTTTATTGCAAAAAGTTATGCCATGGGTAAGATTCCTGGTGGTTTTTTTAAAAGAGAA
GGAAAACCATCTGATAGAGAAACTTTAATTTCAAGAGTAATAGATAGAAGTATAAGGCCGTTATTTCCTGAAGGTTTTCA
TGATGAAATTAGTGTAGTATGTAATCTGTTAACTTACGATACGGTCAATTCTCCTGAAGTACCAGCATTGATAGGTACCG
TCGCAGCTCTTGCAATTTCTGGTGTTCCTTTCCACTTTACTATAGCTGGAGTCATGGTCGGTTGTGATGAAAATAACAAT
TATATACTCAATCCTTCCGTTCAAGAGATGAAAGCGAGCAACTTGGATCTGTTTTTGTCTGGTGATGAAAATTCGATCTT
AATGGTCGAATCGGAAGTCAAAGAGCTCTCTGAAGAAAATGTTTTAAACGCAATAAAATTTGGTCATGAACACCTTCAAC
CTGTTATTAAGCTCATAAAAGAATTTGCTGATACAGTTGGCAATAAACCTGAAAGCTTTGCTCCTGTTGATATATCAGAT
ATAACTCAAGAACTTGAAAAATATCGAAAAGACTTTGAAGAAGCATATTCAAAAACAGTAAAACAAGAGCGAGTCCAAGC
TCTAGAAGTAGTCAGGAATAATATATTGAATACTCTTAAAGAGGCTGGAAAAGACGAAAAGTTAATTACTTATGCAGTAA
AAAGCTTTGAAAGATCTTTAGTGCGTGAAATGATTAGAAGGAAAAGTGTGAGAATAGACAGCCGTAAGTATGATGAAATA
CGCCAGATAGAAATTGAGGCTGACATTCTACCCAAGACTCACGGTTCTGCGCTATTTACAAGGGGTAGCACTCAGGCACT
AGTTGTTACTGCTCTTGGTACTACACAAGATGAGCAAATTGTGGATGACATTGAAGGGGATAGACGTGAACATTTTATGT
TGCATTATAATTTTCCTCCATTTGCTGTTGGAGAGGCTTCTGCTATACGCGCACCAGGAAGGAGAGAAATTGGTCATGGT
AAACTTGCTTGGAAAGCAATTCATCCTGTTTTACCTGATAAATCTGAATTTCCTTATACAATAAGGGTAGTATCTGAGAT
TATGGAATCTGATGGTTCTTCTTCTATGGCAACAGTTTGTGGAACTTCCATTGCATTAATGGATACAGGTGTGCCAATAA
AGGCTCCCGTTGCAGGAATTGCTATGGGTCTCATCAAAGACAAAAATGAGCATATAATACTTTCTGATATACTGGGCGAT
GAAGACTATCTTGGTGATATGGACTTCAAAGTAGCAGGAACTAGCGAAGGGATTACAGCACTGCAGATGGATATGAAAAT
TCCTGGTATAAGCTTTGAAATTGTTGAAAAATCTTTAGAACAAGCAAAAGTCGGAAGATTACATATCTTAGAAAAAATGA
ATGCAGTAATTTCAGAACATCGTAAGGATATAAAAGATCATGTACCAAGAGTGTTATCGTTTTATATAGATAAAGATAAA
ATTTCTGCAGCTATTGGTACTAAAGGAAAAAATATACGCAGTGTATGTGAAAGAAGTAATGCAAAAATTGAAATAGGGGA
TGACGGTAAAGTTTCTGTTTTTGCCATTAGTAGTACTGAAGCTGAAGCTGCAAAGAATATGATGATTGATTCAATAACAG
AGCTAGAACAAGGTTCTATAATTGATGCTAAAGTTGTAAAGATAGAGAAGTCTATTGTAGAGCTAGAACTTCTTAATGGA
AGAAAAGGAAAAATGCATATAAGCGAGGTAGCTAATCAACATGTAGAGTCCATTGAGGATATACTTAAACAGGGTGATAC
CTTCAAAGCTTTGATAATTGACTTTGAAAAAGGTGGATGTCCAAAATTATCAAGACGTCGTGTTGATCAAGAAACAGGTG
AATTTTTTGAAGGTAAGCTTTACAATGAAGAAAGGAGAGATGGTTTAAATAATAGGGATAATTATTATAACAACTCGTTT
AATAAAAAACCTGAAGATAATTATCATAGTAATAGACCTACTCGTCCTCGTTCTGGTTTTAGTAATAGAAGCAGACCAAA
ATTTGGTAATAATGACTCGTCATCAGGTTTCTATTGA

Upstream 100 bases:

>100_bases
GGCGCAAGCACTTAAATTATATAAAACGTAAATTTGGTAATGAAGCCTATCAAGAGTTAATAGAGAAGTTAGGCATTAGA
AAATAATCGAGGAATTTTGT

Downstream 100 bases:

>100_bases
GGTTGAACTTACTTTCTATTATCTCAGTGTCGAAACACTGGGATAATAGGAATTTTTAAAACTGACAAGATAACAAAAAT
GGTTTGTGTTATCTGTAGGG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MFEIIKKSIDWEGRTLSLETGKIARQADGSVVVNYGDTSILVTVVRKKKEESVDFLPLNVQFIAKSYAMGKIPGGFFKRE
GKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYDTVNSPEVPALIGTVAALAISGVPFHFTIAGVMVGCDENNN
YILNPSVQEMKASNLDLFLSGDENSILMVESEVKELSEENVLNAIKFGHEHLQPVIKLIKEFADTVGNKPESFAPVDISD
ITQELEKYRKDFEEAYSKTVKQERVQALEVVRNNILNTLKEAGKDEKLITYAVKSFERSLVREMIRRKSVRIDSRKYDEI
RQIEIEADILPKTHGSALFTRGSTQALVVTALGTTQDEQIVDDIEGDRREHFMLHYNFPPFAVGEASAIRAPGRREIGHG
KLAWKAIHPVLPDKSEFPYTIRVVSEIMESDGSSSMATVCGTSIALMDTGVPIKAPVAGIAMGLIKDKNEHIILSDILGD
EDYLGDMDFKVAGTSEGITALQMDMKIPGISFEIVEKSLEQAKVGRLHILEKMNAVISEHRKDIKDHVPRVLSFYIDKDK
ISAAIGTKGKNIRSVCERSNAKIEIGDDGKVSVFAISSTEAEAAKNMMIDSITELEQGSIIDAKVVKIEKSIVELELLNG
RKGKMHISEVANQHVESIEDILKQGDTFKALIIDFEKGGCPKLSRRRVDQETGEFFEGKLYNEERRDGLNNRDNYYNNSF
NKKPEDNYHSNRPTRPRSGFSNRSRPKFGNNDSSSGFY

Sequences:

>Translated_758_residues
MFEIIKKSIDWEGRTLSLETGKIARQADGSVVVNYGDTSILVTVVRKKKEESVDFLPLNVQFIAKSYAMGKIPGGFFKRE
GKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYDTVNSPEVPALIGTVAALAISGVPFHFTIAGVMVGCDENNN
YILNPSVQEMKASNLDLFLSGDENSILMVESEVKELSEENVLNAIKFGHEHLQPVIKLIKEFADTVGNKPESFAPVDISD
ITQELEKYRKDFEEAYSKTVKQERVQALEVVRNNILNTLKEAGKDEKLITYAVKSFERSLVREMIRRKSVRIDSRKYDEI
RQIEIEADILPKTHGSALFTRGSTQALVVTALGTTQDEQIVDDIEGDRREHFMLHYNFPPFAVGEASAIRAPGRREIGHG
KLAWKAIHPVLPDKSEFPYTIRVVSEIMESDGSSSMATVCGTSIALMDTGVPIKAPVAGIAMGLIKDKNEHIILSDILGD
EDYLGDMDFKVAGTSEGITALQMDMKIPGISFEIVEKSLEQAKVGRLHILEKMNAVISEHRKDIKDHVPRVLSFYIDKDK
ISAAIGTKGKNIRSVCERSNAKIEIGDDGKVSVFAISSTEAEAAKNMMIDSITELEQGSIIDAKVVKIEKSIVELELLNG
RKGKMHISEVANQHVESIEDILKQGDTFKALIIDFEKGGCPKLSRRRVDQETGEFFEGKLYNEERRDGLNNRDNYYNNSF
NKKPEDNYHSNRPTRPRSGFSNRSRPKFGNNDSSSGFY
>Mature_758_residues
MFEIIKKSIDWEGRTLSLETGKIARQADGSVVVNYGDTSILVTVVRKKKEESVDFLPLNVQFIAKSYAMGKIPGGFFKRE
GKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYDTVNSPEVPALIGTVAALAISGVPFHFTIAGVMVGCDENNN
YILNPSVQEMKASNLDLFLSGDENSILMVESEVKELSEENVLNAIKFGHEHLQPVIKLIKEFADTVGNKPESFAPVDISD
ITQELEKYRKDFEEAYSKTVKQERVQALEVVRNNILNTLKEAGKDEKLITYAVKSFERSLVREMIRRKSVRIDSRKYDEI
RQIEIEADILPKTHGSALFTRGSTQALVVTALGTTQDEQIVDDIEGDRREHFMLHYNFPPFAVGEASAIRAPGRREIGHG
KLAWKAIHPVLPDKSEFPYTIRVVSEIMESDGSSSMATVCGTSIALMDTGVPIKAPVAGIAMGLIKDKNEHIILSDILGD
EDYLGDMDFKVAGTSEGITALQMDMKIPGISFEIVEKSLEQAKVGRLHILEKMNAVISEHRKDIKDHVPRVLSFYIDKDK
ISAAIGTKGKNIRSVCERSNAKIEIGDDGKVSVFAISSTEAEAAKNMMIDSITELEQGSIIDAKVVKIEKSIVELELLNG
RKGKMHISEVANQHVESIEDILKQGDTFKALIIDFEKGGCPKLSRRRVDQETGEFFEGKLYNEERRDGLNNRDNYYNNSF
NKKPEDNYHSNRPTRPRSGFSNRSRPKFGNNDSSSGFY

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=659, Percent_Identity=37.1775417298938, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI145693187, Length=702, Percent_Identity=44.5868945868946, Blast_Score=597, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI115534063, Length=654, Percent_Identity=33.0275229357798, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI281362905, Length=652, Percent_Identity=36.6564417177914, Blast_Score=414, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=652, Percent_Identity=36.6564417177914, Blast_Score=414, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=652, Percent_Identity=36.6564417177914, Blast_Score=414, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=598, Percent_Identity=36.2876254180602, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_WOLPP (B3CM21)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975589.1
- EnsemblBacteria:   EBWOLT00000000423
- GeneID:   6384667
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0831
- GeneTree:   EBGT00050000031300
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 84664; Mature: 84664

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEIIKKSIDWEGRTLSLETGKIARQADGSVVVNYGDTSILVTVVRKKKEESVDFLPLNV
CHHHHHHHCCCCCCEEEEECCCHHHCCCCCEEEEECCCEEEHHHHHHHHHCCCCEEECHH
QFIAKSYAMGKIPGGFFKREGKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYD
HHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
TVNSPEVPALIGTVAALAISGVPFHFTIAGVMVGCDENNNYILNPSVQEMKASNLDLFLS
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEECCCHHHHHCCCCEEEEE
GDENSILMVESEVKELSEENVLNAIKFGHEHLQPVIKLIKEFADTVGNKPESFAPVDISD
CCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ITQELEKYRKDFEEAYSKTVKQERVQALEVVRNNILNTLKEAGKDEKLITYAVKSFERSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VREMIRRKSVRIDSRKYDEIRQIEIEADILPKTHGSALFTRGSTQALVVTALGTTQDEQI
HHHHHHHHCCCCCCHHCCCHHEEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCHHHH
VDDIEGDRREHFMLHYNFPPFAVGEASAIRAPGRREIGHGKLAWKAIHPVLPDKSEFPYT
HHHCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEHHHHCCCCCCCCCCCCH
IRVVSEIMESDGSSSMATVCGTSIALMDTGVPIKAPVAGIAMGLIKDKNEHIILSDILGD
HHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCC
EDYLGDMDFKVAGTSEGITALQMDMKIPGISFEIVEKSLEQAKVGRLHILEKMNAVISEH
CCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKDIKDHVPRVLSFYIDKDKISAAIGTKGKNIRSVCERSNAKIEIGDDGKVSVFAISSTE
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCEEEEEEECCH
AEAAKNMMIDSITELEQGSIIDAKVVKIEKSIVELELLNGRKGKMHISEVANQHVESIED
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHH
ILKQGDTFKALIIDFEKGGCPKLSRRRVDQETGEFFEGKLYNEERRDGLNNRDNYYNNSF
HHHCCCCEEEEEEEECCCCCCCHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCC
NKKPEDNYHSNRPTRPRSGFSNRSRPKFGNNDSSSGFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFEIIKKSIDWEGRTLSLETGKIARQADGSVVVNYGDTSILVTVVRKKKEESVDFLPLNV
CHHHHHHHCCCCCCEEEEECCCHHHCCCCCEEEEECCCEEEHHHHHHHHHCCCCEEECHH
QFIAKSYAMGKIPGGFFKREGKPSDRETLISRVIDRSIRPLFPEGFHDEISVVCNLLTYD
HHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
TVNSPEVPALIGTVAALAISGVPFHFTIAGVMVGCDENNNYILNPSVQEMKASNLDLFLS
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEECCCHHHHHCCCCEEEEE
GDENSILMVESEVKELSEENVLNAIKFGHEHLQPVIKLIKEFADTVGNKPESFAPVDISD
CCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ITQELEKYRKDFEEAYSKTVKQERVQALEVVRNNILNTLKEAGKDEKLITYAVKSFERSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VREMIRRKSVRIDSRKYDEIRQIEIEADILPKTHGSALFTRGSTQALVVTALGTTQDEQI
HHHHHHHHCCCCCCHHCCCHHEEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCHHHH
VDDIEGDRREHFMLHYNFPPFAVGEASAIRAPGRREIGHGKLAWKAIHPVLPDKSEFPYT
HHHCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEHHHHCCCCCCCCCCCCH
IRVVSEIMESDGSSSMATVCGTSIALMDTGVPIKAPVAGIAMGLIKDKNEHIILSDILGD
HHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCC
EDYLGDMDFKVAGTSEGITALQMDMKIPGISFEIVEKSLEQAKVGRLHILEKMNAVISEH
CCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKDIKDHVPRVLSFYIDKDKISAAIGTKGKNIRSVCERSNAKIEIGDDGKVSVFAISSTE
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCEEEEEEECCH
AEAAKNMMIDSITELEQGSIIDAKVVKIEKSIVELELLNGRKGKMHISEVANQHVESIED
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHH
ILKQGDTFKALIIDFEKGGCPKLSRRRVDQETGEFFEGKLYNEERRDGLNNRDNYYNNSF
HHHCCCCEEEEEEEECCCCCCCHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCC
NKKPEDNYHSNRPTRPRSGFSNRSRPKFGNNDSSSGFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA