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The map label for this gene is truB

Identifier: 190571229

GI number: 190571229

Start: 905918

End: 906979

Strand: Direct

Name: truB

Synonym: WPa_0829

Alternate gene names: 190571229

Gene position: 905918-906979 (Clockwise)

Preceding gene: 190571227

Following gene: 190571230

Centisome position: 61.11

GC content: 34.84

Gene sequence:

>1062_bases
ATGGTAAATGGCTGGCTGAATCTTGATAAACCAACAGGAATGAGTTCTGCACAAGCTGTAACTCAAATTAAAAGGATTTT
TGGAATAAAAAAAGCCGGACATTTGGGAACACTTGATCCTTTGGCTTCAGGTATATTACCGATCGCTCTTGGTGAAGCAA
CAAAAACTATACCGTATTTATCTTGTGACTTAAAGGCATACAATTTCACAATAAAATGGGGAAAACAAACAACTACGGAT
GATTTGGGTGGTGATATCATTAGAACTAGCGATATAAAACCCGAATATAACCAAATAAACTGTGCGATTAAGGATTTTAT
TGGTGAGATAACACAAACTCCTCCTCAATTTTCAGCAGTGAAAATCAAGGGAGCAAGAGCATATAAATTAGCAAGAAGTG
GGCAAAAGGTAAATATAAAACCTCGACAAGTCAAAATACATGAACTAAAGATGATATTTTTGGACACCATAAATAATATT
GCCGATTTTTCTATGATCTGCGGTAGCGGCGTATATGTAAGATCAATTGCAAGAGATCTTGGAATTGAATTAAATTGTTT
TGGACATATTACACAATTAAGAAGAACGATGGTAGGTGATTTTAAGGAAGATGAATCAGTGACAATTGAGCAACTTACTA
AAAAAAACACAACAACTTACTCTCCCCAGTGCTTGACACTGGATACAAATACTTACAAATCATGTAATGGACAAAAGATT
CTAGGAGCATATGTCAAAAACAATGTTCGTGATGAAATAGGCTTGATCCCAGTGCTTGACACTGGGATGACATCGGAGGG
TGGGATGACAGAAGATGATGGAGGTAATACAAAAGGCTTCATCATTCCCATTGAATCGGCTTTAAAATCGATGTTCAAAG
TTGAAATTTCCTTAGAGGAAGCGGAGAAAATCAGAAAAGGTCAAGAAATTATATTAAATAACTTGCGTAATTTAAAGAAT
TATGATATTTTTTGTACGATAGTGGGTAATGTACCTATTGCAATTTGCAGTTTTACTCATGGTTATGTGAAGCCTATTCG
TGTTTTTAATATTTTGAAATGA

Upstream 100 bases:

>100_bases
TTAATTGTATATTAACAATGCACACTAGTCAAGCATTTTTATTAACAACTTAATATTTTATCTACTTTCTAAATTGTTAC
TAATTATGTAAGATTGATAC

Downstream 100 bases:

>100_bases
GGTTTAGATGTCAATAACATCCCAAAAGAAAAAAAGTTTGATTAGTACATATGCAATTAAAGAAGATGATACAGGTTCAT
CTTTTGTACAGTGTGCAATT

Product: trna pseudouridine synthase b

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MVNGWLNLDKPTGMSSAQAVTQIKRIFGIKKAGHLGTLDPLASGILPIALGEATKTIPYLSCDLKAYNFTIKWGKQTTTD
DLGGDIIRTSDIKPEYNQINCAIKDFIGEITQTPPQFSAVKIKGARAYKLARSGQKVNIKPRQVKIHELKMIFLDTINNI
ADFSMICGSGVYVRSIARDLGIELNCFGHITQLRRTMVGDFKEDESVTIEQLTKKNTTTYSPQCLTLDTNTYKSCNGQKI
LGAYVKNNVRDEIGLIPVLDTGMTSEGGMTEDDGGNTKGFIIPIESALKSMFKVEISLEEAEKIRKGQEIILNNLRNLKN
YDIFCTIVGNVPIAICSFTHGYVKPIRVFNILK

Sequences:

>Translated_353_residues
MVNGWLNLDKPTGMSSAQAVTQIKRIFGIKKAGHLGTLDPLASGILPIALGEATKTIPYLSCDLKAYNFTIKWGKQTTTD
DLGGDIIRTSDIKPEYNQINCAIKDFIGEITQTPPQFSAVKIKGARAYKLARSGQKVNIKPRQVKIHELKMIFLDTINNI
ADFSMICGSGVYVRSIARDLGIELNCFGHITQLRRTMVGDFKEDESVTIEQLTKKNTTTYSPQCLTLDTNTYKSCNGQKI
LGAYVKNNVRDEIGLIPVLDTGMTSEGGMTEDDGGNTKGFIIPIESALKSMFKVEISLEEAEKIRKGQEIILNNLRNLKN
YDIFCTIVGNVPIAICSFTHGYVKPIRVFNILK
>Mature_353_residues
MVNGWLNLDKPTGMSSAQAVTQIKRIFGIKKAGHLGTLDPLASGILPIALGEATKTIPYLSCDLKAYNFTIKWGKQTTTD
DLGGDIIRTSDIKPEYNQINCAIKDFIGEITQTPPQFSAVKIKGARAYKLARSGQKVNIKPRQVKIHELKMIFLDTINNI
ADFSMICGSGVYVRSIARDLGIELNCFGHITQLRRTMVGDFKEDESVTIEQLTKKNTTTYSPQCLTLDTNTYKSCNGQKI
LGAYVKNNVRDEIGLIPVLDTGMTSEGGMTEDDGGNTKGFIIPIESALKSMFKVEISLEEAEKIRKGQEIILNNLRNLKN
YDIFCTIVGNVPIAICSFTHGYVKPIRVFNILK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=231, Percent_Identity=30.3030303030303, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI4503337, Length=214, Percent_Identity=28.9719626168224, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI215599015, Length=214, Percent_Identity=28.9719626168224, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI2367200, Length=213, Percent_Identity=39.4366197183099, Blast_Score=160, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17553978, Length=208, Percent_Identity=31.25, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=162, Percent_Identity=33.9506172839506, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=208, Percent_Identity=28.8461538461538, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_WOLPP (B3CM19)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975587.1
- EnsemblBacteria:   EBWOLT00000001115
- GeneID:   6385563
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0829
- GeneTree:   EBGT00050000031620
- HOGENOM:   HBG397258
- ProtClustDB:   CLSK2516851
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 39053; Mature: 39053

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: ACT_SITE 39-39

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNGWLNLDKPTGMSSAQAVTQIKRIFGIKKAGHLGTLDPLASGILPIALGEATKTIPYL
CCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCEE
SCDLKAYNFTIKWGKQTTTDDLGGDIIRTSDIKPEYNQINCAIKDFIGEITQTPPQFSAV
EEEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
KIKGARAYKLARSGQKVNIKPRQVKIHELKMIFLDTINNIADFSMICGSGVYVRSIARDL
EEECCHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIELNCFGHITQLRRTMVGDFKEDESVTIEQLTKKNTTTYSPQCLTLDTNTYKSCNGQKI
CCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEE
LGAYVKNNVRDEIGLIPVLDTGMTSEGGMTEDDGGNTKGFIIPIESALKSMFKVEISLEE
HHHHHHCCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHEEEEEHHH
AEKIRKGQEIILNNLRNLKNYDIFCTIVGNVPIAICSFTHGYVKPIRVFNILK
HHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHEECC
>Mature Secondary Structure
MVNGWLNLDKPTGMSSAQAVTQIKRIFGIKKAGHLGTLDPLASGILPIALGEATKTIPYL
CCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCEE
SCDLKAYNFTIKWGKQTTTDDLGGDIIRTSDIKPEYNQINCAIKDFIGEITQTPPQFSAV
EEEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
KIKGARAYKLARSGQKVNIKPRQVKIHELKMIFLDTINNIADFSMICGSGVYVRSIARDL
EEECCHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIELNCFGHITQLRRTMVGDFKEDESVTIEQLTKKNTTTYSPQCLTLDTNTYKSCNGQKI
CCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEE
LGAYVKNNVRDEIGLIPVLDTGMTSEGGMTEDDGGNTKGFIIPIESALKSMFKVEISLEE
HHHHHHCCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHEEEEEHHH
AEKIRKGQEIILNNLRNLKNYDIFCTIVGNVPIAICSFTHGYVKPIRVFNILK
HHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA