Click here to switch to the map view.

The map label for this gene is ribD [H]

Identifier: 190571227

GI number: 190571227

Start: 903834

End: 905051

Strand: Direct

Name: ribD [H]

Synonym: WPa_0827

Alternate gene names: 190571227

Gene position: 903834-905051 (Clockwise)

Preceding gene: 190571226

Following gene: 190571229

Centisome position: 60.97

GC content: 37.68

Gene sequence:

>1218_bases
ATGACCGATGATTATTTCATGTCGATCGCATTAAAACTTGCAGAAAAAAGTCTTGGAAGTGTTGCACCAAATCCCGCTGT
TGGATGTATCATTGTAAAAAACGGTATGGTGATCGGTGAAGGCTACACAGGAATCGGTGGACGTCCACATGCAGAAGTAG
TTGCTTTGCAAAATGCTAAAGATTTAACTCATAGTGCAACTATGTACGTCACTCTTGAACCATGCTGCCACTTTGGAGTT
ACAGAACCTTGCACTGCAGAAATCATAAAATCAGGAATAAAAAGAGTAGTAATTGCAGCAATTGATCCAGATAGTAGAGT
TTCAGGTGGAGGAATTAAAGCTCTGAAAGAAGCAGGAATTGAAGTTGAGCAAGGTATTATGCAAAAAGAGGCAGAAGTAT
TGAATGTCGGTTTTTTCACCACTAAAGAATTTCATAGACCATTCATAGCTTGCAAAATCGCAACAACTCTTGACGGAAAA
ATCGCAACATTTACAGGTGATAGCAAATGGATAACAAGTGAAGATACGAGAAATTGGGTACATGAGCTCAGAGCAAAATA
TGATGCAATTATGATTGGCAGTAGTACCCTTATCAATGACGATCCTCTCTTAACTTGCAGATTACCAGGACTCGAAGACA
GGTCGCCAATAAGGCTAATTATCGATAGCCAAGGGAAATTGAAGGAAGAGCATAATATTGCAAAGACTGCAGATACCTTA
TATAACCATGATTTAAGCAAAGAGCCAACAGCATACCTTTCGATGTCATTCCAGCGCGTGACGCTGGAATCTAGAAAAAA
AGAAGAAGAATGGATCCCAGTGTCACGCACTGGGATGACAGGAGGTTCAACTTGGGTAATTACAAACAATAAAGTAAAGA
AAAAAATAAAAAACATTAACTACTTAGTAGTTAATTCAAACAATGCTGGCAAAGTCTGCCTAAAAGACATGGCATCAAAA
CTTGTTTCAGAAATTGGCATAACAAGGTTATTAGTTGAAGGTGGAGGAATATTAATTACAGAGCTATTAAAGCATAATTT
GATTGATAGATTAATAATCTGTCGCAGTGGTAAAATTCTAGGCAATGATGCCACTCCTTTTATAGGAGATCTAGGGATTC
AATCCATAAACAACTGCCATAAATTCAAAAAAGTAAAGATAATAGAGTTCAGTGAGGATGTAGTTGAGGTGTGGGATAGA
TCTTACGTACCTAAATGA

Upstream 100 bases:

>100_bases
CAGTAATAATATGTGATTATATCTCAGGTATGACAGATAGATTTGCCATACACGAGCACAGAAGAATTTTTGATACCTCA
TACGAAATGACTTCTTTCTA

Downstream 100 bases:

>100_bases
TAGAGAAAAGAAGTAGAGATAACTCTACCTCTTTTTTTCTCGATTTAAAACTGCTTACTTCTGAGCAATTTTTGATAGAG
CTGCATTTACTGCAACCTCT

Product: riboflavin biosynthesis protein RibD

Products: NA

Alternate protein names: Diaminohydroxyphosphoribosylaminopyrimidine deaminase; DRAP deaminase; Riboflavin-specific deaminase; 5-amino-6-(5-phosphoribosylamino)uracil reductase; HTP reductase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTDDYFMSIALKLAEKSLGSVAPNPAVGCIIVKNGMVIGEGYTGIGGRPHAEVVALQNAKDLTHSATMYVTLEPCCHFGV
TEPCTAEIIKSGIKRVVIAAIDPDSRVSGGGIKALKEAGIEVEQGIMQKEAEVLNVGFFTTKEFHRPFIACKIATTLDGK
IATFTGDSKWITSEDTRNWVHELRAKYDAIMIGSSTLINDDPLLTCRLPGLEDRSPIRLIIDSQGKLKEEHNIAKTADTL
YNHDLSKEPTAYLSMSFQRVTLESRKKEEEWIPVSRTGMTGGSTWVITNNKVKKKIKNINYLVVNSNNAGKVCLKDMASK
LVSEIGITRLLVEGGGILITELLKHNLIDRLIICRSGKILGNDATPFIGDLGIQSINNCHKFKKVKIIEFSEDVVEVWDR
SYVPK

Sequences:

>Translated_405_residues
MTDDYFMSIALKLAEKSLGSVAPNPAVGCIIVKNGMVIGEGYTGIGGRPHAEVVALQNAKDLTHSATMYVTLEPCCHFGV
TEPCTAEIIKSGIKRVVIAAIDPDSRVSGGGIKALKEAGIEVEQGIMQKEAEVLNVGFFTTKEFHRPFIACKIATTLDGK
IATFTGDSKWITSEDTRNWVHELRAKYDAIMIGSSTLINDDPLLTCRLPGLEDRSPIRLIIDSQGKLKEEHNIAKTADTL
YNHDLSKEPTAYLSMSFQRVTLESRKKEEEWIPVSRTGMTGGSTWVITNNKVKKKIKNINYLVVNSNNAGKVCLKDMASK
LVSEIGITRLLVEGGGILITELLKHNLIDRLIICRSGKILGNDATPFIGDLGIQSINNCHKFKKVKIIEFSEDVVEVWDR
SYVPK
>Mature_404_residues
TDDYFMSIALKLAEKSLGSVAPNPAVGCIIVKNGMVIGEGYTGIGGRPHAEVVALQNAKDLTHSATMYVTLEPCCHFGVT
EPCTAEIIKSGIKRVVIAAIDPDSRVSGGGIKALKEAGIEVEQGIMQKEAEVLNVGFFTTKEFHRPFIACKIATTLDGKI
ATFTGDSKWITSEDTRNWVHELRAKYDAIMIGSSTLINDDPLLTCRLPGLEDRSPIRLIIDSQGKLKEEHNIAKTADTLY
NHDLSKEPTAYLSMSFQRVTLESRKKEEEWIPVSRTGMTGGSTWVITNNKVKKKIKNINYLVVNSNNAGKVCLKDMASKL
VSEIGITRLLVEGGGILITELLKHNLIDRLIICRSGKILGNDATPFIGDLGIQSINNCHKFKKVKIIEFSEDVVEVWDRS
YVPK

Specific function: Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate [H]

COG id: COG0117

COG function: function code H; Pyrimidine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the HTP reductase family [H]

Homologues:

Organism=Escherichia coli, GI1786616, Length=405, Percent_Identity=36.0493827160494, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI145693172, Length=155, Percent_Identity=32.258064516129, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193
- InterPro:   IPR004794
- InterPro:   IPR011549
- InterPro:   IPR002734 [H]

Pfam domain/function: PF00383 dCMP_cyt_deam_1; PF01872 RibD_C [H]

EC number: =3.5.4.26; =1.1.1.193 [H]

Molecular weight: Translated: 44534; Mature: 44403

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDYFMSIALKLAEKSLGSVAPNPAVGCIIVKNGMVIGEGYTGIGGRPHAEVVALQNAK
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCEEEEECCCC
DLTHSATMYVTLEPCCHFGVTEPCTAEIIKSGIKRVVIAAIDPDSRVSGGGIKALKEAGI
CCCCCEEEEEEECCHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHCCC
EVEQGIMQKEAEVLNVGFFTTKEFHRPFIACKIATTLDGKIATFTGDSKWITSEDTRNWV
HHHHHHHHHHHHEEEECEEECHHHCCCEEEEEEEEECCCEEEEEECCCCEECCCHHHHHH
HELRAKYDAIMIGSSTLINDDPLLTCRLPGLEDRSPIRLIIDSQGKLKEEHNIAKTADTL
HHHHHHHCEEEECCCEEECCCCEEEEECCCCCCCCCEEEEECCCCCCHHHCCHHHHHHHH
YNHDLSKEPTAYLSMSFQRVTLESRKKEEEWIPVSRTGMTGGSTWVITNNKVKKKIKNIN
HCCCCCCCCCEEEEEEEEEEEHHHHCCHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCE
YLVVNSNNAGKVCLKDMASKLVSEIGITRLLVEGGGILITELLKHNLIDRLIICRSGKIL
EEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHEEECCCCEE
GNDATPFIGDLGIQSINNCHKFKKVKIIEFSEDVVEVWDRSYVPK
CCCCCCCHHHHCHHHHHHHHHHCEEEEEECCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDDYFMSIALKLAEKSLGSVAPNPAVGCIIVKNGMVIGEGYTGIGGRPHAEVVALQNAK
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCEEEEECCCC
DLTHSATMYVTLEPCCHFGVTEPCTAEIIKSGIKRVVIAAIDPDSRVSGGGIKALKEAGI
CCCCCEEEEEEECCHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHCCC
EVEQGIMQKEAEVLNVGFFTTKEFHRPFIACKIATTLDGKIATFTGDSKWITSEDTRNWV
HHHHHHHHHHHHEEEECEEECHHHCCCEEEEEEEEECCCEEEEEECCCCEECCCHHHHHH
HELRAKYDAIMIGSSTLINDDPLLTCRLPGLEDRSPIRLIIDSQGKLKEEHNIAKTADTL
HHHHHHHCEEEECCCEEECCCCEEEEECCCCCCCCCEEEEECCCCCCHHHCCHHHHHHHH
YNHDLSKEPTAYLSMSFQRVTLESRKKEEEWIPVSRTGMTGGSTWVITNNKVKKKIKNIN
HCCCCCCCCCEEEEEEEEEEEHHHHCCHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCE
YLVVNSNNAGKVCLKDMASKLVSEIGITRLLVEGGGILITELLKHNLIDRLIICRSGKIL
EEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHEEECCCCEE
GNDATPFIGDLGIQSINNCHKFKKVKIIEFSEDVVEVWDRSYVPK
CCCCCCCHHHHCHHHHHHHHHHCEEEEEECCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]