Click here to switch to the map view.

The map label for this gene is ppnK [H]

Identifier: 190571078

GI number: 190571078

Start: 739748

End: 740533

Strand: Direct

Name: ppnK [H]

Synonym: WPa_0673

Alternate gene names: 190571078

Gene position: 739748-740533 (Clockwise)

Preceding gene: 190571077

Following gene: 190571079

Centisome position: 49.9

GC content: 35.11

Gene sequence:

>786_bases
ATGTATAAATATAAAAATGTAGGCTATATTGCTTCTTCGCTACCCAAGTCTCAGGAAGTATCTAAACTACTACAGAAACT
CAATTTTATCAATATAGCAGAAGCAGGTAAGCATGAAGTTGATCTGCTGATAGTTGTTGGTGGTGATGGTTTTATGCTAC
GCACTCTGCACAACTACGTCATAGGAAATAAAGATATACATGTGTATGGAATAAATACCGGTAACGTCGGATTTTTGATG
AATAAATGCTTTGAAGATCTAATTGATCATATAGAACATGCAGTTCCTACTCAGCTAACTTTACTAAAAATGGAAGCCAA
AGACATAAATGGCAAAAAACACCATTACATCGCAGTAAATGAAGTGTATGTCTTTAGAAATGCAAATCAAATAGTGGAGA
TGAATATTACCATTAATGATAAGCTAAAAGTAGAAAAATTCAGAGGAGATGGAATTATATTGTCTACCCCAACAGGTAGC
ACTGCATATAACTTCTCTGCCGGTGGGCCAATCTTACCACTTAATTCAAATTTACTTGCACTGACTTCCATTAATAGCTA
CTACCCAAGGCGTTGGAATGGAGCGCTAATCTCAAATGATACAATAGTGCAAATTGATATCAATGATGTGAAAAATCGTC
CAGCACTTGTAGTATCAGATTACAAGGAATTTCACGACATATCGCAGGTAAAAATACAGAAAGATCACGAAAATACAGTC
ACTCTACTTTTTGACAAAGATTGCCCTTTGAATGAAAGGATCTTTGATCAGCAGTTCTTATACTAA

Upstream 100 bases:

>100_bases
TGGAAGTTTGACAAGCGGTTCAGGGGAGAAGGATAGTAAAATAGCTAAGTTTAATGATAAATACGGGAGTCTTTTTTAGT
TCCTCCCCTCTTTATTTAAC

Downstream 100 bases:

>100_bases
TATTTATTCTTAAATTAGTATTCACTATATCTTAATAACTATAGCGTAATCAACATAACATTGTAATTAATTTTGGAGCG
AATATGCCTGAATCAGGAAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MYKYKNVGYIASSLPKSQEVSKLLQKLNFINIAEAGKHEVDLLIVVGGDGFMLRTLHNYVIGNKDIHVYGINTGNVGFLM
NKCFEDLIDHIEHAVPTQLTLLKMEAKDINGKKHHYIAVNEVYVFRNANQIVEMNITINDKLKVEKFRGDGIILSTPTGS
TAYNFSAGGPILPLNSNLLALTSINSYYPRRWNGALISNDTIVQIDINDVKNRPALVVSDYKEFHDISQVKIQKDHENTV
TLLFDKDCPLNERIFDQQFLY

Sequences:

>Translated_261_residues
MYKYKNVGYIASSLPKSQEVSKLLQKLNFINIAEAGKHEVDLLIVVGGDGFMLRTLHNYVIGNKDIHVYGINTGNVGFLM
NKCFEDLIDHIEHAVPTQLTLLKMEAKDINGKKHHYIAVNEVYVFRNANQIVEMNITINDKLKVEKFRGDGIILSTPTGS
TAYNFSAGGPILPLNSNLLALTSINSYYPRRWNGALISNDTIVQIDINDVKNRPALVVSDYKEFHDISQVKIQKDHENTV
TLLFDKDCPLNERIFDQQFLY
>Mature_261_residues
MYKYKNVGYIASSLPKSQEVSKLLQKLNFINIAEAGKHEVDLLIVVGGDGFMLRTLHNYVIGNKDIHVYGINTGNVGFLM
NKCFEDLIDHIEHAVPTQLTLLKMEAKDINGKKHHYIAVNEVYVFRNANQIVEMNITINDKLKVEKFRGDGIILSTPTGS
TAYNFSAGGPILPLNSNLLALTSINSYYPRRWNGALISNDTIVQIDINDVKNRPALVVSDYKEFHDISQVKIQKDHENTV
TLLFDKDCPLNERIFDQQFLY

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=166, Percent_Identity=39.1566265060241, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320794, Length=204, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322509, Length=172, Percent_Identity=31.9767441860465, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 29574; Mature: 29574

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKYKNVGYIASSLPKSQEVSKLLQKLNFINIAEAGKHEVDLLIVVGGDGFMLRTLHNYV
CCEECCCCHHHHCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCEEEEEHHHHH
IGNKDIHVYGINTGNVGFLMNKCFEDLIDHIEHAVPTQLTLLKMEAKDINGKKHHYIAVN
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEE
EVYVFRNANQIVEMNITINDKLKVEKFRGDGIILSTPTGSTAYNFSAGGPILPLNSNLLA
EEEEEECCCEEEEEEEEECCCEEEEEECCCEEEEECCCCCCEEEECCCCCEEECCCCEEE
LTSINSYYPRRWNGALISNDTIVQIDINDVKNRPALVVSDYKEFHDISQVKIQKDHENTV
EEECCCCCCCCCCCEEECCCEEEEEECCCCCCCCEEEEECCHHHCCCCEEEEEECCCCEE
TLLFDKDCPLNERIFDQQFLY
EEEEECCCCCCCHHCHHHCCC
>Mature Secondary Structure
MYKYKNVGYIASSLPKSQEVSKLLQKLNFINIAEAGKHEVDLLIVVGGDGFMLRTLHNYV
CCEECCCCHHHHCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCEEEEEHHHHH
IGNKDIHVYGINTGNVGFLMNKCFEDLIDHIEHAVPTQLTLLKMEAKDINGKKHHYIAVN
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEE
EVYVFRNANQIVEMNITINDKLKVEKFRGDGIILSTPTGSTAYNFSAGGPILPLNSNLLA
EEEEEECCCEEEEEEEEECCCEEEEEECCCEEEEECCCCCCEEEECCCCCEEECCCCEEE
LTSINSYYPRRWNGALISNDTIVQIDINDVKNRPALVVSDYKEFHDISQVKIQKDHENTV
EEECCCCCCCCCCCEEECCCEEEEEECCCCCCCCEEEEECCHHHCCCCEEEEEECCCCEE
TLLFDKDCPLNERIFDQQFLY
EEEEECCCCCCCHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]