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The map label for this gene is alaS [H]

Identifier: 190571072

GI number: 190571072

Start: 732239

End: 734821

Strand: Direct

Name: alaS [H]

Synonym: WPa_0667

Alternate gene names: 190571072

Gene position: 732239-734821 (Clockwise)

Preceding gene: 190571065

Following gene: 190571074

Centisome position: 49.39

GC content: 35.19

Gene sequence:

>2583_bases
ATGAAGCTAAACGAAATTAGAGAAAGATTTATAAAATTTTTTGTAAATAATAATCACGAGCAAGTTCCTTCTTCTCCTTT
GATTCCAGAGCATGATCCAACACTCATGTTTACAAATGCTGGTATGGTACAGTTCAAAAACATCTTTACCGGCACGCAAA
AAACTGAAATGAAACGTGCCGTCTCAAGTCAAAAGTGTCTCAGAGCAGGTGGTAAACACAATGATCTTGAAAATGTTGGC
TATACATCTCGGCATCACACATTTTTTGAAATGCTCGGAAATTTTAGCTTTGGTGATTACTTTAAAGAAACTGCGATAGA
ATTTGCGTGGAAATTTATTACTGAAGAATTGTCTCTTGATAAAAACAGACTATCCATAACTGTTTACCACACTGATGATG
AAGCATATGAAATTTGGCGTAAGATAAGTGGCTTTTCAGATGATAAAATCATAAGAATTACAACAGATGATAACTTTTGG
AGCATGGGCAATACTGGTCCATGTGGCCCATGTTCTGAAATCTTTTACGATCATGGAAATTCCGCTTTACATGAAGACGA
CAGAATTGTTGAAATTTGGAACCTGGTGTTCATGGAATTTAATAAAGATGAAGAAGGTAATTTACAAAAATTACCAAAAA
AATGCATCGATACCGGAATGGGCCTTGAAAGAATAGCAGCTGTTATGCAGAACGTTCATGATAACTATGATATTGACCTA
TTTTCTGCTCTGGTAAGTAAATCGCAAGAGTACTGTGGAAATACGGAAAATAAAGTAGCTCATAAGATTATCGCAGATCA
CCTTCGTGCAGCTGCATTTCTCATCGCAGAAGGAGTGCTTCCTGGAAATGAAGGAAGAAATTATGTACTACGCAGATTGA
TCAGGAGAGCAGCACGTTATATCCACCTACTTGGATATAATGATTCTCTATTGAACCTTGTTTTTCCTGCACTCATAGAT
GGCACAAGTTTGGCTTATATGGGAGATGCTTATCCAGAGCTAATCAGAGCTAAAAGCTTAATAGAAACAACGTTAAAATC
AGAGGAAGAAAACTTTAAAGACACTTTAATGAAAGGCATTAATCTCTTGGACAAATTCACTGCAGATTTAAAATCAGGTG
ACACTCTATCTGGAGAATCAGCATTCAAACTATATGACACTTATGGATTTCCTTTGGATATCACACTTGATATTTTGAAA
GAAAGGAAAATAAATTTTGACCAAAAAGGTTTTGATAATGCAATGAAAGAGCAAAAAGAAAGAGCACGTGCTAATTGGAC
TGGATCTGGTGAAAAATCTGTTGAACAAGTATGGTTTAATTTAATCGATCAGTTTGGCAAAACGGAATTTGTTGGTTATG
AACGTGATGAAGTAAAGGATGCAACAGTACTCGCAATAATTTCCTCTAAAAATGAAGTAATTGATTCTGCAAAAGAAGGA
GAAAAGATAACTATTATACTTGATAAAACACCTTTTTATGGAGAGTCAGGTGGACAGGTGGGAGATACTGGAAATTTTAT
AAAGTCTGATGGAAGTACGTTAACAGTGGAAAATACCAACAAGATTAACGACCTATATTTACACAGATGTATAGTTGAAT
CTGGTTCAGTTCGTAAAGGTGACACAGTTACAGCTAGTATTAATAAAAAAAGAAGACAAGACTTAAGAAGAAATCATTCA
GCTACACATCTTCTACACTTTGCACTCAGAAAAATCTTAGGTGATCACGTTACTCAAAAAGGTTCTCTAGTCGCACCAAA
TAGACTAAGATTTGACTTTAGTCACAATAAGCCAGTCACTCAGGATCAGCTGTCTTCAGTAGAAGATATAGTAAACTCTC
TAATCAGAGAAAATCTTTCTACGTCCACAAAAGTTCAAGGTATGGATGAGGCAATAGACGAAGGAGCGATGGCCTTATTT
GGTGAAAAATATGGTGATAATGTTAGGGTTGTAAAAATTGGAGATTCAAAAGAATTATGTGGTGGTACACACGTGGAGCG
TACCGGAGAAATTGGTTTGTTTAAGATAGCAGCAGAATGTTCCGTTGCATCTCGAGTAAGAAGAATTGAAGCTTTAACCG
GTCAAGAAGCAATTAATTATGTACGCAATAACGAAATTAGCTTAAAAAAAGTTGCAGAATGCGTAAAAGCACCAGTAAAT
GAAATAATAAGTCGACTCAGTATTTTAAGCCAGGAGCGCAAAGAATTTGAAGTCAAAATAAAAAATCTTTATAAAAAGCT
TATCAGTGTAGAAACTGTAAAAAGTACCGAAATAAATGGAATAAATTTCGTAAGCCACACTTTCAATAACGTTCCAGGAA
GTATAATAAGGGAATTTGCTCTACAGCAACAAAAACCGAAAACTGTAATAGCCTTCATGGTAATAGAAAAGGATAAAACA
GTTTTGATTGTTAAAGTAAGTAAAGATTTAACTAATAAGATCAATGCAAAAGAGCTAGTATCTACTTTAACCAGAAGGGA
TTGCGGTGGAAATGCCGAACTTGCCCAAACAGGCTGTGATAACAATAAAATAGATGATGCCATTACAGCAATCCATAGCA
AAATAACAGCTTGCAAAAACTAA

Upstream 100 bases:

>100_bases
CTTTTATTATACTAAGTAATGTATATATTAATATTTAGATTGATAAATTGATTTTACTTCTGTTACAACTTATTATGCAT
AGATAACCTCAGTAAAAACG

Downstream 100 bases:

>100_bases
TACACCCTACTACCTCGATATCTTTTAACCGTAAACGAAGGTATTATGCAAATAGGGGTAGAGAAGACTTAACTGTCAAA
TGAGCATATCAGGTAATAAG

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MKLNEIRERFIKFFVNNNHEQVPSSPLIPEHDPTLMFTNAGMVQFKNIFTGTQKTEMKRAVSSQKCLRAGGKHNDLENVG
YTSRHHTFFEMLGNFSFGDYFKETAIEFAWKFITEELSLDKNRLSITVYHTDDEAYEIWRKISGFSDDKIIRITTDDNFW
SMGNTGPCGPCSEIFYDHGNSALHEDDRIVEIWNLVFMEFNKDEEGNLQKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL
FSALVSKSQEYCGNTENKVAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARYIHLLGYNDSLLNLVFPALID
GTSLAYMGDAYPELIRAKSLIETTLKSEEENFKDTLMKGINLLDKFTADLKSGDTLSGESAFKLYDTYGFPLDITLDILK
ERKINFDQKGFDNAMKEQKERARANWTGSGEKSVEQVWFNLIDQFGKTEFVGYERDEVKDATVLAIISSKNEVIDSAKEG
EKITIILDKTPFYGESGGQVGDTGNFIKSDGSTLTVENTNKINDLYLHRCIVESGSVRKGDTVTASINKKRRQDLRRNHS
ATHLLHFALRKILGDHVTQKGSLVAPNRLRFDFSHNKPVTQDQLSSVEDIVNSLIRENLSTSTKVQGMDEAIDEGAMALF
GEKYGDNVRVVKIGDSKELCGGTHVERTGEIGLFKIAAECSVASRVRRIEALTGQEAINYVRNNEISLKKVAECVKAPVN
EIISRLSILSQERKEFEVKIKNLYKKLISVETVKSTEINGINFVSHTFNNVPGSIIREFALQQQKPKTVIAFMVIEKDKT
VLIVKVSKDLTNKINAKELVSTLTRRDCGGNAELAQTGCDNNKIDDAITAIHSKITACKN

Sequences:

>Translated_860_residues
MKLNEIRERFIKFFVNNNHEQVPSSPLIPEHDPTLMFTNAGMVQFKNIFTGTQKTEMKRAVSSQKCLRAGGKHNDLENVG
YTSRHHTFFEMLGNFSFGDYFKETAIEFAWKFITEELSLDKNRLSITVYHTDDEAYEIWRKISGFSDDKIIRITTDDNFW
SMGNTGPCGPCSEIFYDHGNSALHEDDRIVEIWNLVFMEFNKDEEGNLQKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL
FSALVSKSQEYCGNTENKVAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARYIHLLGYNDSLLNLVFPALID
GTSLAYMGDAYPELIRAKSLIETTLKSEEENFKDTLMKGINLLDKFTADLKSGDTLSGESAFKLYDTYGFPLDITLDILK
ERKINFDQKGFDNAMKEQKERARANWTGSGEKSVEQVWFNLIDQFGKTEFVGYERDEVKDATVLAIISSKNEVIDSAKEG
EKITIILDKTPFYGESGGQVGDTGNFIKSDGSTLTVENTNKINDLYLHRCIVESGSVRKGDTVTASINKKRRQDLRRNHS
ATHLLHFALRKILGDHVTQKGSLVAPNRLRFDFSHNKPVTQDQLSSVEDIVNSLIRENLSTSTKVQGMDEAIDEGAMALF
GEKYGDNVRVVKIGDSKELCGGTHVERTGEIGLFKIAAECSVASRVRRIEALTGQEAINYVRNNEISLKKVAECVKAPVN
EIISRLSILSQERKEFEVKIKNLYKKLISVETVKSTEINGINFVSHTFNNVPGSIIREFALQQQKPKTVIAFMVIEKDKT
VLIVKVSKDLTNKINAKELVSTLTRRDCGGNAELAQTGCDNNKIDDAITAIHSKITACKN
>Mature_860_residues
MKLNEIRERFIKFFVNNNHEQVPSSPLIPEHDPTLMFTNAGMVQFKNIFTGTQKTEMKRAVSSQKCLRAGGKHNDLENVG
YTSRHHTFFEMLGNFSFGDYFKETAIEFAWKFITEELSLDKNRLSITVYHTDDEAYEIWRKISGFSDDKIIRITTDDNFW
SMGNTGPCGPCSEIFYDHGNSALHEDDRIVEIWNLVFMEFNKDEEGNLQKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL
FSALVSKSQEYCGNTENKVAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARYIHLLGYNDSLLNLVFPALID
GTSLAYMGDAYPELIRAKSLIETTLKSEEENFKDTLMKGINLLDKFTADLKSGDTLSGESAFKLYDTYGFPLDITLDILK
ERKINFDQKGFDNAMKEQKERARANWTGSGEKSVEQVWFNLIDQFGKTEFVGYERDEVKDATVLAIISSKNEVIDSAKEG
EKITIILDKTPFYGESGGQVGDTGNFIKSDGSTLTVENTNKINDLYLHRCIVESGSVRKGDTVTASINKKRRQDLRRNHS
ATHLLHFALRKILGDHVTQKGSLVAPNRLRFDFSHNKPVTQDQLSSVEDIVNSLIRENLSTSTKVQGMDEAIDEGAMALF
GEKYGDNVRVVKIGDSKELCGGTHVERTGEIGLFKIAAECSVASRVRRIEALTGQEAINYVRNNEISLKKVAECVKAPVN
EIISRLSILSQERKEFEVKIKNLYKKLISVETVKSTEINGINFVSHTFNNVPGSIIREFALQQQKPKTVIAFMVIEKDKT
VLIVKVSKDLTNKINAKELVSTLTRRDCGGNAELAQTGCDNNKIDDAITAIHSKITACKN

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=788, Percent_Identity=38.8324873096447, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI38569417, Length=821, Percent_Identity=32.6431181485993, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1789048, Length=863, Percent_Identity=46.234067207416, Blast_Score=773, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=769, Percent_Identity=37.7113133940182, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17536681, Length=755, Percent_Identity=33.6423841059603, Blast_Score=382, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324911, Length=809, Percent_Identity=40.4202719406675, Blast_Score=562, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24582809, Length=979, Percent_Identity=34.4228804902962, Blast_Score=494, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45552267, Length=979, Percent_Identity=34.4228804902962, Blast_Score=494, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24658214, Length=812, Percent_Identity=30.6650246305419, Blast_Score=352, Evalue=9e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96833; Mature: 96833

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNEIRERFIKFFVNNNHEQVPSSPLIPEHDPTLMFTNAGMVQFKNIFTGTQKTEMKRA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHH
VSSQKCLRAGGKHNDLENVGYTSRHHTFFEMLGNFSFGDYFKETAIEFAWKFITEELSLD
HHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KNRLSITVYHTDDEAYEIWRKISGFSDDKIIRITTDDNFWSMGNTGPCGPCSEIFYDHGN
CCEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHCCC
SALHEDDRIVEIWNLVFMEFNKDEEGNLQKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL
CCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH
FSALVSKSQEYCGNTENKVAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHE
IHLLGYNDSLLNLVFPALIDGTSLAYMGDAYPELIRAKSLIETTLKSEEENFKDTLMKGI
EEEECCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NLLDKFTADLKSGDTLSGESAFKLYDTYGFPLDITLDILKERKINFDQKGFDNAMKEQKE
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RARANWTGSGEKSVEQVWFNLIDQFGKTEFVGYERDEVKDATVLAIISSKNEVIDSAKEG
HHHCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCHHHCCCCEEEEEECCCHHHHHHHCCC
EKITIILDKTPFYGESGGQVGDTGNFIKSDGSTLTVENTNKINDLYLHRCIVESGSVRKG
CEEEEEEECCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
DTVTASINKKRRQDLRRNHSATHLLHFALRKILGDHVTQKGSLVAPNRLRFDFSHNKPVT
CEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCC
QDQLSSVEDIVNSLIRENLSTSTKVQGMDEAIDEGAMALFGEKYGDNVRVVKIGDSKELC
HHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHC
GGTHVERTGEIGLFKIAAECSVASRVRRIEALTGQEAINYVRNNEISLKKVAECVKAPVN
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EIISRLSILSQERKEFEVKIKNLYKKLISVETVKSTEINGINFVSHTFNNVPGSIIREFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCHHHHHHHH
LQQQKPKTVIAFMVIEKDKTVLIVKVSKDLTNKINAKELVSTLTRRDCGGNAELAQTGCD
HHCCCCCEEEEEEEEECCCEEEEEEECCHHHHCCCHHHHHHHHHHHCCCCCCHHHHCCCC
NNKIDDAITAIHSKITACKN
CCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLNEIRERFIKFFVNNNHEQVPSSPLIPEHDPTLMFTNAGMVQFKNIFTGTQKTEMKRA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHH
VSSQKCLRAGGKHNDLENVGYTSRHHTFFEMLGNFSFGDYFKETAIEFAWKFITEELSLD
HHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KNRLSITVYHTDDEAYEIWRKISGFSDDKIIRITTDDNFWSMGNTGPCGPCSEIFYDHGN
CCEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHHCCC
SALHEDDRIVEIWNLVFMEFNKDEEGNLQKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL
CCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH
FSALVSKSQEYCGNTENKVAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHE
IHLLGYNDSLLNLVFPALIDGTSLAYMGDAYPELIRAKSLIETTLKSEEENFKDTLMKGI
EEEECCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NLLDKFTADLKSGDTLSGESAFKLYDTYGFPLDITLDILKERKINFDQKGFDNAMKEQKE
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RARANWTGSGEKSVEQVWFNLIDQFGKTEFVGYERDEVKDATVLAIISSKNEVIDSAKEG
HHHCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCHHHCCCCEEEEEECCCHHHHHHHCCC
EKITIILDKTPFYGESGGQVGDTGNFIKSDGSTLTVENTNKINDLYLHRCIVESGSVRKG
CEEEEEEECCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
DTVTASINKKRRQDLRRNHSATHLLHFALRKILGDHVTQKGSLVAPNRLRFDFSHNKPVT
CEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCC
QDQLSSVEDIVNSLIRENLSTSTKVQGMDEAIDEGAMALFGEKYGDNVRVVKIGDSKELC
HHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHC
GGTHVERTGEIGLFKIAAECSVASRVRRIEALTGQEAINYVRNNEISLKKVAECVKAPVN
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EIISRLSILSQERKEFEVKIKNLYKKLISVETVKSTEINGINFVSHTFNNVPGSIIREFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCHHHHHHHH
LQQQKPKTVIAFMVIEKDKTVLIVKVSKDLTNKINAKELVSTLTRRDCGGNAELAQTGCD
HHCCCCCEEEEEEEEECCCEEEEEEECCHHHHCCCHHHHHHHHHHHCCCCCCHHHHCCCC
NNKIDDAITAIHSKITACKN
CCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA