Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 190570583

GI number: 190570583

Start: 121673

End: 123202

Strand: Reverse

Name: metG [H]

Synonym: WPa_0118

Alternate gene names: 190570583

Gene position: 123202-121673 (Counterclockwise)

Preceding gene: 190570584

Following gene: 190570581

Centisome position: 8.31

GC content: 37.58

Gene sequence:

>1530_bases
ATGGAGCAATTTGAGAGCTTTTACATCACTACGCCAATATATTACGTAAACGACAAGCCACATATCGGCCACGCATATAC
TTCCCTTATCTGTGATGTTGCAGCTAGATTTATGAAATTAGCTGGAAAGAACGTCAAATTTACTACTGGTACAGATGAAC
ATGGACAAAAAATTGAAAAAGCAGCTAAAACAAAGGGAATAGAGCCAAAAGAATTTACAGATGAAATAAGTGTTACGTTC
AGAAAATTAGCTGAGCTAATGAACTTTGATTATGATGATTTTATTCGCACCACAGAAGAACGTCATGAAAAAGCAGTTAT
AGCTCTGTGGAATAGGCTTGAAGAGAGAGGGCAAATATATCTGGATTCTTACTCAGGTTGGTATTCAGTTCGTGATGAAG
CGTTTTATCAGGAATCAGAGTTGATATATGGCAGAGCGCCAACAGGTGCTGAAGTTCAGTGGATAAAAGAAGAGAGCTAC
TTTTTTCGTTTATCAAGTTGGCAAAATAAATTACTAGAGTTATACGAAAATCAGCCGAATTTCATCTTTCCTGAGAGCAG
AAAAAACGAGGTGGTATCGTTTGTAAAATCAGGACTTACTGACCTTTCGATTTCTCGTACTAGTTTTAATTGGGGAATAA
AAGTACCAGAGAATGACAAGCACGTAATTTATGTTTGGATAGATGCGCTCACTAACTATCTCACATCAATAGGCTTTCCT
AATATAGAAGATGAGGAATATAAGAAGTTTTGGACAAACTCCTTCAACGTTCATGTGATCGGTAAAGACATATTGCGTTT
TCACGCTGTATACTGGCCAGCTATTCTTCTTGCAGCAGATTTACCACTGCCAAAGCAAATTGCAGTTCATGGTTGGTGGC
TAAACGAGGGGGAAAAAATATCCAAATCCCTTGGTAACGTTATAGATCCAATTAATCTTGCAGAAGAGTTTGGTGTTGAT
CAACTACGCTATTTTCTCCTTCGGGAAGCAAGTTTCGGTCAAGATGGTAATTTCAGTAAGAAGAACATGATCAGCCGCAT
AAACTCAGAGCTGGCAAACAATATAGGCAATTTAGTGCAAAGAACAATTTCATTTCTGCACAAGCAATGCTTTGGAGTTG
TACCAGTGGTCGACCGCAATTTGCTTAAAGATGATGAGAGTTTACCAAATTGCAAAGCCATAATTGATCAAGTAATGGAT
CATCTATCAAAGTATGAATTTAACCAGATTATACTCCTGATTATCAATATCTCTTCCAAGGCCAATGCTTACATAGATAA
AAGTGCACCCTGGACACTCAGTAAAACCGATAGAGAGCGCATGGATTTAGTAATCTACAAGTTACTTGAATACATTAGAA
TGATTGGCATTTTGCTGCAGCCAATTGTTCCAAAGTCAGCAAAAATGATACTCGATCAGTTGCAAATTCCAAAAGAACAA
CGTGATTTAAAATCTCTGTGCAACGCATGTGTAAGCTTAGGCATTACACTGCCTAAACCTACGCCGATTTTTTTGAGGTT
TGATATTTAA

Upstream 100 bases:

>100_bases
CGCGCAGCGGTATAATGGATTTTCAGATTGCTTGTCTGGTAGGATATGTATTGATTTTTATTACTAGTGCAGATACGATA
AAGCTTTAAACTAGTACAAA

Downstream 100 bases:

>100_bases
AGAACCCTTAGTAAACCAGGCTGAATTGGTCGTTGTGTAGTTAATTTGCTTAGTTCAACAGAATCTAACAAAGGTACCTT
GCTGTTTTGATGTGTTTATA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MEQFESFYITTPIYYVNDKPHIGHAYTSLICDVAARFMKLAGKNVKFTTGTDEHGQKIEKAAKTKGIEPKEFTDEISVTF
RKLAELMNFDYDDFIRTTEERHEKAVIALWNRLEERGQIYLDSYSGWYSVRDEAFYQESELIYGRAPTGAEVQWIKEESY
FFRLSSWQNKLLELYENQPNFIFPESRKNEVVSFVKSGLTDLSISRTSFNWGIKVPENDKHVIYVWIDALTNYLTSIGFP
NIEDEEYKKFWTNSFNVHVIGKDILRFHAVYWPAILLAADLPLPKQIAVHGWWLNEGEKISKSLGNVIDPINLAEEFGVD
QLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISFLHKQCFGVVPVVDRNLLKDDESLPNCKAIIDQVMD
HLSKYEFNQIILLIINISSKANAYIDKSAPWTLSKTDRERMDLVIYKLLEYIRMIGILLQPIVPKSAKMILDQLQIPKEQ
RDLKSLCNACVSLGITLPKPTPIFLRFDI

Sequences:

>Translated_509_residues
MEQFESFYITTPIYYVNDKPHIGHAYTSLICDVAARFMKLAGKNVKFTTGTDEHGQKIEKAAKTKGIEPKEFTDEISVTF
RKLAELMNFDYDDFIRTTEERHEKAVIALWNRLEERGQIYLDSYSGWYSVRDEAFYQESELIYGRAPTGAEVQWIKEESY
FFRLSSWQNKLLELYENQPNFIFPESRKNEVVSFVKSGLTDLSISRTSFNWGIKVPENDKHVIYVWIDALTNYLTSIGFP
NIEDEEYKKFWTNSFNVHVIGKDILRFHAVYWPAILLAADLPLPKQIAVHGWWLNEGEKISKSLGNVIDPINLAEEFGVD
QLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISFLHKQCFGVVPVVDRNLLKDDESLPNCKAIIDQVMD
HLSKYEFNQIILLIINISSKANAYIDKSAPWTLSKTDRERMDLVIYKLLEYIRMIGILLQPIVPKSAKMILDQLQIPKEQ
RDLKSLCNACVSLGITLPKPTPIFLRFDI
>Mature_509_residues
MEQFESFYITTPIYYVNDKPHIGHAYTSLICDVAARFMKLAGKNVKFTTGTDEHGQKIEKAAKTKGIEPKEFTDEISVTF
RKLAELMNFDYDDFIRTTEERHEKAVIALWNRLEERGQIYLDSYSGWYSVRDEAFYQESELIYGRAPTGAEVQWIKEESY
FFRLSSWQNKLLELYENQPNFIFPESRKNEVVSFVKSGLTDLSISRTSFNWGIKVPENDKHVIYVWIDALTNYLTSIGFP
NIEDEEYKKFWTNSFNVHVIGKDILRFHAVYWPAILLAADLPLPKQIAVHGWWLNEGEKISKSLGNVIDPINLAEEFGVD
QLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISFLHKQCFGVVPVVDRNLLKDDESLPNCKAIIDQVMD
HLSKYEFNQIILLIINISSKANAYIDKSAPWTLSKTDRERMDLVIYKLLEYIRMIGILLQPIVPKSAKMILDQLQIPKEQ
RDLKSLCNACVSLGITLPKPTPIFLRFDI

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=511, Percent_Identity=38.3561643835616, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI14043022, Length=523, Percent_Identity=26.5774378585086, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788432, Length=500, Percent_Identity=26, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI212645096, Length=501, Percent_Identity=37.5249500998004, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71992781, Length=393, Percent_Identity=35.6234096692112, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17540794, Length=550, Percent_Identity=26.3636363636364, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321610, Length=527, Percent_Identity=36.0531309297913, Blast_Score=331, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6321704, Length=516, Percent_Identity=24.8062015503876, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21358483, Length=499, Percent_Identity=38.6773547094188, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24655311, Length=552, Percent_Identity=25.9057971014493, Blast_Score=145, Evalue=9e-35,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58925; Mature: 58925

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQFESFYITTPIYYVNDKPHIGHAYTSLICDVAARFMKLAGKNVKFTTGTDEHGQKIEK
CCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
AAKTKGIEPKEFTDEISVTFRKLAELMNFDYDDFIRTTEERHEKAVIALWNRLEERGQIY
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LDSYSGWYSVRDEAFYQESELIYGRAPTGAEVQWIKEESYFFRLSSWQNKLLELYENQPN
EECCCCCEEHHHHHHHCCCCEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCC
FIFPESRKNEVVSFVKSGLTDLSISRTSFNWGIKVPENDKHVIYVWIDALTNYLTSIGFP
EECCCCCHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCEEEEEEHHHHHHHHHHCCCC
NIEDEEYKKFWTNSFNVHVIGKDILRFHAVYWPAILLAADLPLPKQIAVHGWWLNEGEKI
CCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHH
SKSLGNVIDPINLAEEFGVDQLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQ
HHHHHHHCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTISFLHKQCFGVVPVVDRNLLKDDESLPNCKAIIDQVMDHLSKYEFNQIILLIINISSK
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
ANAYIDKSAPWTLSKTDRERMDLVIYKLLEYIRMIGILLQPIVPKSAKMILDQLQIPKEQ
CCEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHH
RDLKSLCNACVSLGITLPKPTPIFLRFDI
HHHHHHHHHHHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MEQFESFYITTPIYYVNDKPHIGHAYTSLICDVAARFMKLAGKNVKFTTGTDEHGQKIEK
CCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
AAKTKGIEPKEFTDEISVTFRKLAELMNFDYDDFIRTTEERHEKAVIALWNRLEERGQIY
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LDSYSGWYSVRDEAFYQESELIYGRAPTGAEVQWIKEESYFFRLSSWQNKLLELYENQPN
EECCCCCEEHHHHHHHCCCCEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCC
FIFPESRKNEVVSFVKSGLTDLSISRTSFNWGIKVPENDKHVIYVWIDALTNYLTSIGFP
EECCCCCHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCEEEEEEHHHHHHHHHHCCCC
NIEDEEYKKFWTNSFNVHVIGKDILRFHAVYWPAILLAADLPLPKQIAVHGWWLNEGEKI
CCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHH
SKSLGNVIDPINLAEEFGVDQLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQ
HHHHHHHCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTISFLHKQCFGVVPVVDRNLLKDDESLPNCKAIIDQVMDHLSKYEFNQIILLIINISSK
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
ANAYIDKSAPWTLSKTDRERMDLVIYKLLEYIRMIGILLQPIVPKSAKMILDQLQIPKEQ
CCEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHH
RDLKSLCNACVSLGITLPKPTPIFLRFDI
HHHHHHHHHHHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA