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The map label for this gene is prfA

Identifier: 190570518

GI number: 190570518

Start: 38128

End: 39219

Strand: Direct

Name: prfA

Synonym: WPa_0043

Alternate gene names: 190570518

Gene position: 38128-39219 (Clockwise)

Preceding gene: 190570515

Following gene: 190570519

Centisome position: 2.57

GC content: 35.71

Gene sequence:

>1092_bases
ATGGATATAGAGAATAATTTACAAGACTTAAAAAAAAAGTTTTCTGATGTAGAGAGAAATCTGGAAAACCCTACCAATTT
GAGTCAAAAAGAATTCGTTAGTTTTTCAAAGGAATACTCTGAACTCAGACCAATTATCGAGATAATCGATGAATATAATA
TATTAAAAGAGGAAATTTCAGATTTAGAAGAAATCATGAAAGATGAGAACAGCGATGGTGATATAAAAGAGTTAGCAAAA
GAAGAACTTCTTGAGAAGCAAAAGATAGTATTACCGAAAGTAAAAGCAAAACTAAAGTTGGCATTATTACCCAAAGATGA
AGATGACTCAAGAAATGCAATATTAGAAATTAGAGCAGGCACAGGCGGAGAAGAAGCAGCATTATTTGCAGCGATGTTAT
TTCGTATGTATCAAAAATATGCAGAAAGAAGAAATTGGAAGTTTGAGCCAATAAGTATTTCTAATACAGGTATAGGTGGC
TATAAGGAAGCTTCTGCACTCATTAATGGAACAGAAGTTTTTGCAAGACTGAAATTTGAGTCAGGGGTGCACAGAGTACA
AAGAGTGCCAGAAACTGAATCCTCAGGAAGATTGCACACTTCCGCAGCTACTGTTGCAATATTACCTGAAGTAGAAGAGG
TTGACTTTGAAATAGAAGAAAAAGATTTACGCATAGATGTTTATAGATCCAGTGGTCCTGGAGGGCAATCAGTGAATACA
ACTGACAGCGCAGTAAGGGTCACCCACTTGCCAACAGGGATAGTTGTAATACAGCAAGATGAAAAATCGCAGCACAAAAA
TAAGGCTAAAGCGCTCAAAGTGTTGAGAGCAAGGCTATACGAAATTGAAAGACAAAAAAAAGAAATGGAAAGGTCAACAA
TGAGGAAAAGCCAAATTGGTTCTGGAGATCGCTCTGAACGTATAAGAACTTATAACTTTCCACAATCAAGAATAACAGAT
CATAGAATTAATCTCACTTCACATCGACTAGAACAGATTATAAAAGAAGGTGAACTAGATGAATTTATTGAGGCATTGAT
TTCACGAAATGAAGCAGAAAGGTTGACTGGAGGGGGTAATGTTACTTTTTGA

Upstream 100 bases:

>100_bases
AAATATTCGAGACAATTATAGTGTTATTGCTTGATATAAGAAATTTTTTATTTCAGTATATATTACTTACTTAAACAGCT
GAAAACTAGACCTGTACTAT

Downstream 100 bases:

>100_bases
TGGAAATTTGAAACAAAGCATAGGTATCAGGTTAAAAAGAAGTAAAGTTAGTTGTTCATAAGGTTGACAATTATTTAAAT
TTATGCTATAATTAGCAGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MDIENNLQDLKKKFSDVERNLENPTNLSQKEFVSFSKEYSELRPIIEIIDEYNILKEEISDLEEIMKDENSDGDIKELAK
EELLEKQKIVLPKVKAKLKLALLPKDEDDSRNAILEIRAGTGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGG
YKEASALINGTEVFARLKFESGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFEIEEKDLRIDVYRSSGPGGQSVNT
TDSAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSGDRSERIRTYNFPQSRITD
HRINLTSHRLEQIIKEGELDEFIEALISRNEAERLTGGGNVTF

Sequences:

>Translated_363_residues
MDIENNLQDLKKKFSDVERNLENPTNLSQKEFVSFSKEYSELRPIIEIIDEYNILKEEISDLEEIMKDENSDGDIKELAK
EELLEKQKIVLPKVKAKLKLALLPKDEDDSRNAILEIRAGTGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGG
YKEASALINGTEVFARLKFESGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFEIEEKDLRIDVYRSSGPGGQSVNT
TDSAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSGDRSERIRTYNFPQSRITD
HRINLTSHRLEQIIKEGELDEFIEALISRNEAERLTGGGNVTF
>Mature_363_residues
MDIENNLQDLKKKFSDVERNLENPTNLSQKEFVSFSKEYSELRPIIEIIDEYNILKEEISDLEEIMKDENSDGDIKELAK
EELLEKQKIVLPKVKAKLKLALLPKDEDDSRNAILEIRAGTGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGG
YKEASALINGTEVFARLKFESGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFEIEEKDLRIDVYRSSGPGGQSVNT
TDSAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSGDRSERIRTYNFPQSRITD
HRINLTSHRLEQIIKEGELDEFIEALISRNEAERLTGGGNVTF

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=308, Percent_Identity=48.7012987012987, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI34577120, Length=360, Percent_Identity=38.8888888888889, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=47.8672985781991, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=50.7082152974504, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=39.8230088495575, Blast_Score=220, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=308, Percent_Identity=37.987012987013, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=42.7272727272727, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=41.6918429003021, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_WOLPP (B3CL87)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001974876.1
- EnsemblBacteria:   EBWOLT00000000222
- GeneID:   6385016
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0043
- GeneTree:   EBGT00050000030962
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41454; Mature: 41454

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIENNLQDLKKKFSDVERNLENPTNLSQKEFVSFSKEYSELRPIIEIIDEYNILKEEIS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLEEIMKDENSDGDIKELAKEELLEKQKIVLPKVKAKLKLALLPKDEDDSRNAILEIRAG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEEEEECC
TGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGGYKEASALINGTEVFARLKFE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
SGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFEIEEKDLRIDVYRSSGPGGQSVNT
HHHHHHHHCCCCCCCCCEEECCEEEEECCCHHHCCCEEECCCEEEEEEECCCCCCCCCCC
TDSAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIG
CCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRSERIRTYNFPQSRITDHRINLTSHRLEQIIKEGELDEFIEALISRNEAERLTGGGN
CCCCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCC
VTF
CCC
>Mature Secondary Structure
MDIENNLQDLKKKFSDVERNLENPTNLSQKEFVSFSKEYSELRPIIEIIDEYNILKEEIS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLEEIMKDENSDGDIKELAKEELLEKQKIVLPKVKAKLKLALLPKDEDDSRNAILEIRAG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEEEEECC
TGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGGYKEASALINGTEVFARLKFE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
SGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFEIEEKDLRIDVYRSSGPGGQSVNT
HHHHHHHHCCCCCCCCCEEECCEEEEECCCHHHCCCEEECCCEEEEEEECCCCCCCCCCC
TDSAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIG
CCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRSERIRTYNFPQSRITDHRINLTSHRLEQIIKEGELDEFIEALISRNEAERLTGGGN
CCCCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCC
VTF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA