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The map label for this gene is purA [H]

Identifier: 190570514

GI number: 190570514

Start: 33147

End: 34424

Strand: Reverse

Name: purA [H]

Synonym: WPa_0039

Alternate gene names: 190570514

Gene position: 34424-33147 (Counterclockwise)

Preceding gene: 190570516

Following gene: 190570513

Centisome position: 2.32

GC content: 35.52

Gene sequence:

>1278_bases
ATGAATAATATTGTAATTGTTGGTCTGCAATGGGGTGACGAAGGCAAGGGTAAAATAGTAGATTATCTTTCTGAGAATGC
AGATGTAGTTGTGAGATTTCAGGGAGGAAATAATGCAGGGCACACTATAGTAATAGATGATGAGGTTTATAAATTAAATT
TACTGCCCTCTGCTGTTTTGAGAGCGGACAAAATATCTATTATAGGAAACGGTGTTGCTCTTGATTCACATGCTCTAGTC
TCAGAAATAGAGTCATTGAAAGTTAAAGGAGTAGACGTAAACTATAACAACTTGATGGTATCCGAGAGCTGTCCATTAAT
ACTTAGCGTACATAAGGACAAGGAAAAGCTATTTGAAGATTTAAACGGAAATCACAAAATTGGTACAACAAACAAAGGGA
TAGGGCCATGTTATGAAGATAAAGTTGGCAGGAGAGCTATACGCCTTTGTGACTTAGAAAACACAGATGAGCTCAATCAA
AGAGTGGATGCTCTCCTGAGTTACCATAATGCTATCAGAAAAGGACTTAACTACCAGGTAGTTAAAAAAGAAGAAATATT
AAAGGAAATTCAAGAGATTTCAGAAAAAATTCTTTTATATAAAAAACCTGTGTGGAAGATACTAAATGATTTTATGAAAG
AAGGTAAGAAAATAATATTTGAAGGCGCTCAAGGCGCATTTTTAGATATCGACCACGGAACTTACCCTTTTGTTACTTCA
AGTAATACCGTAGCATCGCAAGCGATAACAGGCTCAGGATTATCCTACAATGCTCAAATTATTGGTGTAGCAAAAGCGTA
TACAACAAGAGTGGGTAATGGTCCATTTCCTACTGAACAAAATAACGAGATTGGAGACAGCTTATTTTCTATAGGTAAGG
AACTTGGAACAGTAAGCAATAGAAGAAGGCGCTGCGGATGGTTTGACGCAGTTTTAGTGCGCCAGGCCGTACAACTCTCT
GGAGTTTCAAGCATTGTATTAACCAAATTAGATATTCTTGATTCTTTTGATACTATTAAAATAGGCACTGGTTATAAGTA
TGGCGGAAAAATGTATGATTATTTACCCGCATCACATTCAATACAAAAAGAATTAGAGCCAATATATGAAGAATTTCCTG
GTTGGAAAGAGAAAACTCAAGGCAAAAGGTCAGTTAAGGAATTACCTGCAAATTTGATGAAATATGTGAGAAAAATAGAA
GAATTAATAGGTGTACCAATTCATCTAATTTCAACTAGTCCCAATAGAGAGGATGTTATTAAGCTTAAAAATCTATGA

Upstream 100 bases:

>100_bases
AGTCTATTTCTTTATTTTAGGGATATAGGAAAGGCGTAGATTTCTCTTTTCATTTGTAGCATGCTGCATTAATATTTACG
TATTAATCTAGAAAATAGTG

Downstream 100 bases:

>100_bases
AAACGTCTTGTTAACTTTTATCTAATAAAAAACGATACTTGTGTGACTTCAAGCAATCTATTATGCAAAGTAGCAGCGTA
AACGTACAGTGAGCAGTGTG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVIDDEVYKLNLLPSAVLRADKISIIGNGVALDSHALV
SEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFEDLNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENTDELNQ
RVDALLSYHNAIRKGLNYQVVKKEEILKEIQEISEKILLYKKPVWKILNDFMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
SNTVASQAITGSGLSYNAQIIGVAKAYTTRVGNGPFPTEQNNEIGDSLFSIGKELGTVSNRRRRCGWFDAVLVRQAVQLS
GVSSIVLTKLDILDSFDTIKIGTGYKYGGKMYDYLPASHSIQKELEPIYEEFPGWKEKTQGKRSVKELPANLMKYVRKIE
ELIGVPIHLISTSPNREDVIKLKNL

Sequences:

>Translated_425_residues
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVIDDEVYKLNLLPSAVLRADKISIIGNGVALDSHALV
SEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFEDLNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENTDELNQ
RVDALLSYHNAIRKGLNYQVVKKEEILKEIQEISEKILLYKKPVWKILNDFMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
SNTVASQAITGSGLSYNAQIIGVAKAYTTRVGNGPFPTEQNNEIGDSLFSIGKELGTVSNRRRRCGWFDAVLVRQAVQLS
GVSSIVLTKLDILDSFDTIKIGTGYKYGGKMYDYLPASHSIQKELEPIYEEFPGWKEKTQGKRSVKELPANLMKYVRKIE
ELIGVPIHLISTSPNREDVIKLKNL
>Mature_425_residues
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVIDDEVYKLNLLPSAVLRADKISIIGNGVALDSHALV
SEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFEDLNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENTDELNQ
RVDALLSYHNAIRKGLNYQVVKKEEILKEIQEISEKILLYKKPVWKILNDFMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
SNTVASQAITGSGLSYNAQIIGVAKAYTTRVGNGPFPTEQNNEIGDSLFSIGKELGTVSNRRRRCGWFDAVLVRQAVQLS
GVSSIVLTKLDILDSFDTIKIGTGYKYGGKMYDYLPASHSIQKELEPIYEEFPGWKEKTQGKRSVKELPANLMKYVRKIE
ELIGVPIHLISTSPNREDVIKLKNL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=40.9411764705882, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=40.5152224824356, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=39.3939393939394, Blast_Score=282, Evalue=5e-76,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=45.4545454545455, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25146555, Length=422, Percent_Identity=41.2322274881517, Blast_Score=314, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI25146553, Length=422, Percent_Identity=41.2322274881517, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=39.4859813084112, Blast_Score=303, Evalue=4e-83,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=39.8104265402844, Blast_Score=314, Evalue=9e-86,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47272; Mature: 47272

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVIDDEVYKLNLLPSAVL
CCCEEEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCHHHHH
RADKISIIGNGVALDSHALVSEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFED
HHCCEEEEECCEEECHHHHHHHHHHCEEEEEEECCCCEEEECCCCEEEEECCCHHHHHHH
LNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENTDELNQRVDALLSYHNAIRKGLNYQV
CCCCCEECCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
VKKEEILKEIQEISEKILLYKKPVWKILNDFMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
ECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVASQAITGSGLSYNAQIIGVAKAYTTRVGNGPFPTEQNNEIGDSLFSIGKELGTVSN
CCCHHHHHCCCCCCEECEEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRRRCGWFDAVLVRQAVQLSGVSSIVLTKLDILDSFDTIKIGTGYKYGGKMYDYLPASHS
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCH
IQKELEPIYEEFPGWKEKTQGKRSVKELPANLMKYVRKIEELIGVPIHLISTSPNREDVI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCE
KLKNL
EECCC
>Mature Secondary Structure
MNNIVIVGLQWGDEGKGKIVDYLSENADVVVRFQGGNNAGHTIVIDDEVYKLNLLPSAVL
CCCEEEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCHHHHH
RADKISIIGNGVALDSHALVSEIESLKVKGVDVNYNNLMVSESCPLILSVHKDKEKLFED
HHCCEEEEECCEEECHHHHHHHHHHCEEEEEEECCCCEEEECCCCEEEEECCCHHHHHHH
LNGNHKIGTTNKGIGPCYEDKVGRRAIRLCDLENTDELNQRVDALLSYHNAIRKGLNYQV
CCCCCEECCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
VKKEEILKEIQEISEKILLYKKPVWKILNDFMKEGKKIIFEGAQGAFLDIDHGTYPFVTS
ECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVASQAITGSGLSYNAQIIGVAKAYTTRVGNGPFPTEQNNEIGDSLFSIGKELGTVSN
CCCHHHHHCCCCCCEECEEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRRRCGWFDAVLVRQAVQLSGVSSIVLTKLDILDSFDTIKIGTGYKYGGKMYDYLPASHS
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCH
IQKELEPIYEEFPGWKEKTQGKRSVKELPANLMKYVRKIEELIGVPIHLISTSPNREDVI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCE
KLKNL
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA