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The map label for this gene is trmE [H]

Identifier: 189499228

GI number: 189499228

Start: 239908

End: 241329

Strand: Direct

Name: trmE [H]

Synonym: Cphamn1_0248

Alternate gene names: 189499228

Gene position: 239908-241329 (Clockwise)

Preceding gene: 189499227

Following gene: 189499229

Centisome position: 8.77

GC content: 56.75

Gene sequence:

>1422_bases
ATGAATGAACCAGTTTCCCTTCCTCTCCAGGGTGAGCCGATTGCCGCTGTTGCTACTCCGGTAGGGGTCGGCGCGCTTGC
TGTGGTCAGGATGAGCGGTGAGGGTGTTTTTTCTATCGCGGACCGGGTCTTTACCAAGGTTCGTTCGCCGGAAACATCTT
TGGCTGACTCTCCTGGGTATACCGCCCATTTCGGGAAGTTGTATGACGGCGACAGGATGATTGACGAGGTGATCGTGCTG
GTGTTCCGGTCACCTGATTCTTTCACGGTCGAGGATATGGTCGAGATCACCTGTCACGGCGGTCCTGTGGTGACAAAGCA
TGTGCTGAAGCTTCTGCTCGACAACGGATGCAGGCTCGCAGAGCCTGGCGAGTTTACCCGACGCGCATTCCTGAACGGGA
GGATCGACCTCTTGCAGGCTGAAGCGATCGGTGAAATGATTCATGCCCGCTCGGAATCGGCATATCGGACCGCGGTAAAC
CAGATGAGCGGAGGGCTTTCCGGGAGGCTCGGCACATTGCGTGAACAGCTTCTGCATTCCTGCGCGCTGCTTGAACTGGA
ACTTGATTTCAGTGAAGAGGATGTCGAATTCCAGAGCAGGGAGGAGTTGAGCGGGCAGGTAGAAAATCTGCAACAGGAAG
TCGACAAGCTCGTGCAGTCCTATCAGCACGGCAGGTTGCTGCGCGAGGGCGTGGCGACCGCCATTATCGGGAGGCCCAAT
GCGGGTAAATCAACCCTGCTCAACGCCTTGCTCGGCGAGGAACGGGCCATTGTCAGCCACATGCCGGGCACCACGAGGGA
CTATATCGAGGAGTGTTTCATCTATGAAAAAACCATGTTCCGGCTTACCGATACCGCCGGACTCCGCGAGGCGGCTGAAG
AGATCGAGCACGAGGGAATCCGGCGCAGCTACGAGAAAATCGCCGAAGCCGACCTGATCCTCTATCTGCTGGATATCGGA
GCCTCCGATTATGAGGAGGAGACCAGTACTGTTACCGAGCTGCGCCAAAAGCATCCCGACATTCAACTGCTGGTGGTTGC
GAACAAGACCGATACCACAGAAGACAGCGCTGAGCGCTCAGAAAAAGTTGCCGAAAAAACCGGTTGTCAGGTGCTCGGTA
TTTCAGCCCTGAAAGAGGATGGTCTCGATCGGTTGAAGGCCGGGATGAGCAGCATGGTCGAGGGACTGGACAAGCTGCAC
GACGCGAGCGTGCTTGTCACCAGCATGCGCCACTACGAGGCCCTGCGCAACGCCTCGGATGCGCTACGCAACGCCCGGGA
ACTGATTGACGCGCAATCGGAAACCGAACTCATAGCCTTCGAACTCCGCTCCGCCCTCGACTACGTCGGCGAGATTACCG
GTAAGGTTGTTAACGAGGAGGTTCTTAATGTCATCTTCGGGCAGTTCTGTATCGGGAAGTGA

Upstream 100 bases:

>100_bases
GGGGATGTTGCCTCTCTGATCCTTACTATGATTGAACTTCCTGATGACATGCTGCCGGATCAGGTGGTTGTCCGCCCACT
GTAAACAGAACGCACATACC

Downstream 100 bases:

>100_bases
GTGCAAAAACGGAAAAGACGAAAAAGACTTGCAATGCAAATGACGTAAGGGGCTCAAAAGGCTTCGTTATTTTTCTTCAA
AGTAACTTAGAGTCAGGTTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNEPVSLPLQGEPIAAVATPVGVGALAVVRMSGEGVFSIADRVFTKVRSPETSLADSPGYTAHFGKLYDGDRMIDEVIVL
VFRSPDSFTVEDMVEITCHGGPVVTKHVLKLLLDNGCRLAEPGEFTRRAFLNGRIDLLQAEAIGEMIHARSESAYRTAVN
QMSGGLSGRLGTLREQLLHSCALLELELDFSEEDVEFQSREELSGQVENLQQEVDKLVQSYQHGRLLREGVATAIIGRPN
AGKSTLLNALLGEERAIVSHMPGTTRDYIEECFIYEKTMFRLTDTAGLREAAEEIEHEGIRRSYEKIAEADLILYLLDIG
ASDYEEETSTVTELRQKHPDIQLLVVANKTDTTEDSAERSEKVAEKTGCQVLGISALKEDGLDRLKAGMSSMVEGLDKLH
DASVLVTSMRHYEALRNASDALRNARELIDAQSETELIAFELRSALDYVGEITGKVVNEEVLNVIFGQFCIGK

Sequences:

>Translated_473_residues
MNEPVSLPLQGEPIAAVATPVGVGALAVVRMSGEGVFSIADRVFTKVRSPETSLADSPGYTAHFGKLYDGDRMIDEVIVL
VFRSPDSFTVEDMVEITCHGGPVVTKHVLKLLLDNGCRLAEPGEFTRRAFLNGRIDLLQAEAIGEMIHARSESAYRTAVN
QMSGGLSGRLGTLREQLLHSCALLELELDFSEEDVEFQSREELSGQVENLQQEVDKLVQSYQHGRLLREGVATAIIGRPN
AGKSTLLNALLGEERAIVSHMPGTTRDYIEECFIYEKTMFRLTDTAGLREAAEEIEHEGIRRSYEKIAEADLILYLLDIG
ASDYEEETSTVTELRQKHPDIQLLVVANKTDTTEDSAERSEKVAEKTGCQVLGISALKEDGLDRLKAGMSSMVEGLDKLH
DASVLVTSMRHYEALRNASDALRNARELIDAQSETELIAFELRSALDYVGEITGKVVNEEVLNVIFGQFCIGK
>Mature_473_residues
MNEPVSLPLQGEPIAAVATPVGVGALAVVRMSGEGVFSIADRVFTKVRSPETSLADSPGYTAHFGKLYDGDRMIDEVIVL
VFRSPDSFTVEDMVEITCHGGPVVTKHVLKLLLDNGCRLAEPGEFTRRAFLNGRIDLLQAEAIGEMIHARSESAYRTAVN
QMSGGLSGRLGTLREQLLHSCALLELELDFSEEDVEFQSREELSGQVENLQQEVDKLVQSYQHGRLLREGVATAIIGRPN
AGKSTLLNALLGEERAIVSHMPGTTRDYIEECFIYEKTMFRLTDTAGLREAAEEIEHEGIRRSYEKIAEADLILYLLDIG
ASDYEEETSTVTELRQKHPDIQLLVVANKTDTTEDSAERSEKVAEKTGCQVLGISALKEDGLDRLKAGMSSMVEGLDKLH
DASVLVTSMRHYEALRNASDALRNARELIDAQSETELIAFELRSALDYVGEITGKVVNEEVLNVIFGQFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=482, Percent_Identity=34.6473029045643, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI193082988, Length=514, Percent_Identity=32.8793774319066, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI193082993, Length=473, Percent_Identity=33.615221987315, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI2367268, Length=463, Percent_Identity=36.9330453563715, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082120, Length=177, Percent_Identity=29.9435028248588, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=30.1075268817204, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=30.1810865191147, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45550813, Length=421, Percent_Identity=33.0166270783848, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 52064; Mature: 52064

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPVSLPLQGEPIAAVATPVGVGALAVVRMSGEGVFSIADRVFTKVRSPETSLADSPGY
CCCCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
TAHFGKLYDGDRMIDEVIVLVFRSPDSFTVEDMVEITCHGGPVVTKHVLKLLLDNGCRLA
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHCCCCCC
EPGEFTRRAFLNGRIDLLQAEAIGEMIHARSESAYRTAVNQMSGGLSGRLGTLREQLLHS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
CALLELELDFSEEDVEFQSREELSGQVENLQQEVDKLVQSYQHGRLLREGVATAIIGRPN
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
AGKSTLLNALLGEERAIVSHMPGTTRDYIEECFIYEKTMFRLTDTAGLREAAEEIEHEGI
CCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRSYEKIAEADLILYLLDIGASDYEEETSTVTELRQKHPDIQLLVVANKTDTTEDSAERS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHH
EKVAEKTGCQVLGISALKEDGLDRLKAGMSSMVEGLDKLHDASVLVTSMRHYEALRNASD
HHHHHHCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRNARELIDAQSETELIAFELRSALDYVGEITGKVVNEEVLNVIFGQFCIGK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEPVSLPLQGEPIAAVATPVGVGALAVVRMSGEGVFSIADRVFTKVRSPETSLADSPGY
CCCCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
TAHFGKLYDGDRMIDEVIVLVFRSPDSFTVEDMVEITCHGGPVVTKHVLKLLLDNGCRLA
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHCCCCCC
EPGEFTRRAFLNGRIDLLQAEAIGEMIHARSESAYRTAVNQMSGGLSGRLGTLREQLLHS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
CALLELELDFSEEDVEFQSREELSGQVENLQQEVDKLVQSYQHGRLLREGVATAIIGRPN
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
AGKSTLLNALLGEERAIVSHMPGTTRDYIEECFIYEKTMFRLTDTAGLREAAEEIEHEGI
CCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRSYEKIAEADLILYLLDIGASDYEEETSTVTELRQKHPDIQLLVVANKTDTTEDSAERS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHH
EKVAEKTGCQVLGISALKEDGLDRLKAGMSSMVEGLDKLHDASVLVTSMRHYEALRNASD
HHHHHHCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRNARELIDAQSETELIAFELRSALDYVGEITGKVVNEEVLNVIFGQFCIGK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]